Martin DruckerFrench National Institute for Agriculture Nutrition Environment
Martin Drucker
Doctor of Philosophy
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100
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Introduction
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Publications
Publications (100)
Multi-infection of plants by viruses is very common and can change drastically infection parameters such as virus accumulation, distribution, and vector transmission. Sugar beet is an important crop that is frequently co-infected by the polerovirus beet chlorosis virus (BChV) and the closterovirus beet yellows virus (BYV), both vectored by the gree...
Bemisia tabaci New World (NW) (Gennadius) (Hemiptera: Aleyrodidae), a whitefly in the B. tabaci species complex, is polyphagous on many plant species. Yet, it has been displaced, albeit not entirely, by other whitefly species. Potential causes could include issues with adaptation, feeding, and the colonization of new-hosts; however, insights that w...
Multi-infection of plant by viruses is very common and can change drastically infection parameters such as virus accumulation, distribution and vector transmission. Sugar beet is an important crop that is frequently co-infected by the polerovirus beet chlorosis virus (BChV) and the closterovirus beet yellows virus (BYV), both vectored by the green...
There is growing evidence that plant viruses manipulate their hosts and vectors in ways that increase transmission. However, to date only few viral components underlying these phenomena have been identified. Here we show that cauliflower mosaic virus (CaMV) protein P2 modifies the feeding behavior of its aphid vector. P2 is necessary for CaMV trans...
Background: Numerous studies have documented modifications in vector orientation behavior, settling and feeding behavior, and/or fecundity and survival due to virus infection in host plants. These alterations are often expected to enhance virus transmission, which has led to the hypothesis that such effects are vector manipulations by the virus. Ho...
We used the NanoLuc luciferase bioluminescent reporter system to detect turnip yellows virus (TuYV) in infected plants. For this, TuYV was genetically tagged by replacing the C-terminal part of the RT protein with full-length NanoLuc (TuYV-NL) or with the N-terminal domain of split NanoLuc (TuYV-N65-NL). Wild-type and recombinant viruses were agro-...
Plant viruses change the phenotype of their plant hosts. Some of the changes impact interactions of the plant with insects that feed on the plants and transmit these viruses. These modifications may result in better virus transmission. We examine here the transcriptomes of two plant species infected with two viruses with different transmission mode...
Background
Numerous studies have documented modifications in vector orientation behavior, settling and feeding behavior, and/or fecundity and survival due to virus infection in host plants. These alterations are often expected to enhance virus transmission, which has led to the hypothesis that such effects are vector manipulations by the virus. How...
Background
Evidence accumulates that plant viruses alter host-plant traits in ways that modify their insect vectors’ behavior. These alterations often enhance virus transmission, which has led to the hypothesis that these effects are manipulations caused by viral adaptation. However, the genetic basis of these indirect, plant-mediated effects on ve...
Alighting aphids probe a new host plant by intracellular test punctures for suitability. These induce immediate calcium signals that emanate from the punctured sites and might be the first step in plant recognition of aphid feeding and the subsequent elicitation of plant defence responses. Calcium is also involved in the transmission of non-persist...
Studying in vivo feeding and other behaviors of small insects, such as aphids, is important for understanding their lifecycle and interaction with the environment. In this regard, the EPG (electrical penetration graph) technique is widely used to study the feeding activity in aphids. However, it is restricted to recording feeding of single insects...
Emerging evidence suggests that viral infection modifies host plant traits that in turn alter behaviour and performance of vectors colonizing the plants in a way conducive for transmission of both nonpersistent and persistent viruses. Similar evidence for semipersistent viruses like cauliflower mosaic virus (CaMV) is scarce. Here we compared the ef...
Cauliflower mosaic virus (CaMV) is transmitted by aphids using the non-circulative transmission mode: when the insects feed on infected leaves, virus particles from infected cells attach rapidly to their stylets and are transmitted to a new host when the aphids change plants. Mandatory for CaMV transmission, the viral helper protein P2 mediates as...
During the process of virus acquisition by aphids, plants respond to both the virus and the aphids by mobilizing different metabolic pathways. It is conceivable that the plant metabolic responses to both aggressors may be conducive to virus acquisition. To address this question, we analyze the accumulation of the phloem-limited polerovirus Turnip y...
Turnip mosaic virus (TuMV, family Potyviridae) and cauliflower mosaic virus (CaMV, family Caulimoviridae) are transmitted by aphid vectors. They are the only viruses shown so far to undergo transmission activation (TA) immediately preceding plant-to-plant propagation. TA is a recently described phenomenon where viruses respond to the presence of ve...
Cauliflower mosaic virus (CaMV, family Caulimoviridae ) responds to the presence of aphid vectors on infected plants by forming specific transmission morphs. This phenomenon, coined transmission activation (TA), controls plant-to-plant propagation of CaMV. A fundamental question is whether other viruses rely on TA. Here, we demonstrate that transmi...
The split GFP technique is based on the auto-assembly of GFP when two polypeptides–GFP1-10 (residues 1–214; the detector) and GFP11 (residues 215–230; the tag)–both non-fluorescing on their own, associate spontaneously to form a fluorescent molecule. We evaluated this technique for its efficacy in contributing to the characterization of Cauliflower...
Detection of encapsidated DNA.
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Plant viruses that are transmitted in a non-circulative, semi-persistent (NCSP) manner have determinants on, and/or accessories to, their capsids that facilitate virion binding to specific retention sites in their insect vectors. Bilateral interactions and interactions occurring at the nexus of all three partners (virus, vector and plant) also cont...
Transmission of plant viruses by phytophagous hemipteran insects encompasses complex interactions underlying a continuum of processes involved in virus acquisition, retention and inoculation combined with vector feeding behavior. Here, we investigated the effects of dietary pH on whitefly (Bemisia tabaci) feeding behavior and release of Lettuce inf...
By serving as vectors of transmission, insects play a key role in the infection cycle of many plant viruses. Viruses use sophisticated transmission strategies to overcome the spatial barrier separating plants and the impediment imposed by the plant cell wall. Interactions among insect vectors, viruses and host plants mediate transmission by integra...
Significance
Autophagy contributes to innate immune responses in metazoans by targeted elimination of intracellular pathogens, including viruses, in a process termed “xenophagy.” Whether autophagy has a similar role in plant immunity is unknown. Here we demonstrate that the selective autophagy receptor NEIGHBOR OF BRCA1 (NBR1) binds the viral capsi...
Many viruses form inclusion bodies in infected plant and mamma- lian cells. Their formation often requires membrane rearrangement of various organelles, but some inclusions form in the cytoplasm independently of the endo- membrane system. In the latter case, they may resemble aggresomes or stress bodies but many inclusions do not seem to be related...
Many viruses form inclusion bodies in infected plant and mammalian cells. Their formation often requires membrane rearrangement of various organelles, but some inclusions form in the cytoplasm independently of the endomembrane system. In the latter case, they may resemble aggresomes or stress bodies but many inclusions do not seem to be related to...
The mechanisms and impacts of the transmission of plant viruses by insect vectors have been studied for more than a century. The virus route within the insect vector is amply documented in many cases, but the identity, the biochemical properties, and the structure of the actual molecules (or molecule domains) ensuring compatibility between them rem...
Cauliflower mosaic virus (CaMV) forms two types of inclusion bodies within infected plant cells: numerous virus factories, which are the sites for viral replication and virion assembly, and a single transmission body (TB), which is specialized for virus transmission by aphid vectors. The TB reacts within seconds to aphid feeding on the host plant b...
Aphids infest many plants and cause damage by depriving them of nutrients and by transmitting many viral diseases. Aphid infestation and arbovirus transmission are controlled by establishment (or not) of a compatible reaction between the insects and the plants. This reaction is the result of defense reactions of the plant and counter-defense reacti...
Confocal single sections and acquisition parameters for Figure 2E.DOI:
http://dx.doi.org/10.7554/eLife.00183.009
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http://dx.doi.org/10.7554/eLife.00183.015
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Confocal single sections and acquisition parameters for Figure 5ADOI:
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Confocal single sections and acquisition parameters used for Figure 2A.DOI:
http://dx.doi.org/10.7554/eLife.00183.005
Confocal single sections and acquisition parameters for Figure 2C.DOI:
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Confocal single sections and acquisition parameters for Figure 2F.DOI:
http://dx.doi.org/10.7554/eLife.00183.010
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http://dx.doi.org/10.7554/eLife.00183.013
Confocal single sections and acquisition parameters for Figure 5BDOI:
http://dx.doi.org/10.7554/eLife.00183.022
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Confocal projection and acquisition parameters for Figure 6A.DOI:
http://dx.doi.org/10.7554/eLife.00183.028
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Confocal single sections and acquisition parameters used for Figure 2B.DOI:
http://dx.doi.org/10.7554/eLife.00183.006
Confocal single sections and acquisition parameters for Figure 2D.DOI:
http://dx.doi.org/10.7554/eLife.00183.008
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Confocal single sections and acquisition parameters for Figure 9F.DOI:
http://dx.doi.org/10.7554/eLife.00183.044
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http://dx.doi.org/10.7554/eLife.00183.055
Confocal single section and acquisition parameters for Figure 6C.DOI:
http://dx.doi.org/10.7554/eLife.00183.030
Confocal single sections and acquisition parameters for Figure 8A.DOI:
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Confocal single sections and acquisition parameters for Figure 8D.DOI:
http://dx.doi.org/10.7554/eLife.00183.036
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Confocal single sections and acquisition parameters for Figure 10B.DOI:
http://dx.doi.org/10.7554/eLife.00183.050
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Confocal single sections and acquisition parameters for Figure 10F.DOI:
http://dx.doi.org/10.7554/eLife.00183.054
Confocal single section and acquisition parameters for Figure 6B.DOI:
http://dx.doi.org/10.7554/eLife.00183.029
Confocal single sections and acquisition parameters for Figure 8B.DOI:
http://dx.doi.org/10.7554/eLife.00183.035
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Abstract
Many plant and animal viruses are spread by insect vectors. Cauliflower mosaic virus (CaMV) is aphid-transmitted, with the virus being taken up from specialized transmission bodies (TB) formed within infected plant cells. However, the precise events during TB-mediated virus acquisition
by aphids are unknown. Here, we show that TBs react in...
Mechanical vector-less transmission of viruses, as well as vector-mediated non-circulative virus transmission, where the virus attaches only to the exterior of the vector during the passage to a new host, are apparently simple processes: the viruses are carried along with the wind, the food or by the vector to a new host. We discuss here, using the...
Host-to-host transmission--a key step in plant virus infection cycles--is ensured predominantly by vectors, especially aphids and related insects. A deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strategies, because any newly discovered molecular or ce...
Understanding the mechanisms controlling vector-transmission of plant viruses requires integrating information from at least three different viewpoints: virus-vector interactions, plant-vector interactions and virus-plant interactions. While some of these aspects have been covered by past and present investigations, others have been bypassed comple...
Transmission by a vector is a common feature among viruses, especially plant viruses. While animal arboviruses infect literally their vector ("biological transmission"), plant viruses are mostly transmitted "mechanically". This mode of transmission is seemingly quite simple - the virus contaminates the vector mouthparts and subsequently is mechanic...
Transmission by a vector is a common feature among viruses, especially plant viruses. While animal arboviruses infect literally their vector ("biological transmission"), plant viruses are mostly transmitted "mechanically". This mode of transmission is seemingly quite simple - the virus contaminates the vector mouthparts and subsequently is mechanic...
UMR BGPI Equipe 2 Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699
Cauliflower mosaic virus (CaMV) is transmitted from plant to plant through a seemingly simple interaction with insect vectors. This process involves
an aphid receptor and two viral proteins, P2 and P3. P2 binds to both the aphid receptor and P3, itself tightly associated
with the virus particle, with the ensemble forming a transmissible viral compl...
Though the duration of a single round of replication is an important biological parameter, it has been determined for only few viruses. Here, this parameter was determined for Cauliflower mosaic virus (CaMV) in transfected protoplasts from different hosts: the highly susceptible Arabidopsis and turnip, and Nicotiana benthamiana, where CaMV accumula...
Transmission of plant viruses is the result of interactions between a given virus, the host plant, and the vector. Most research has focused on molecular and cellular virus-vector interactions, and the host has only been regarded as a reservoir from which the virus is acquired by the vector more or less accidentally. However, a growing body of evid...
Transmission of plant viruses is the result of interactions between a given virus, the host plant, and the vector. Most research has focused on molecular and cellular virus-vector interactions, and the host has only been regarded as a reservoir from which the virus is acquired by the vector more or less accidentally. However, a growing body of evid...
Interactions between microtubules and viruses play important roles in viral infection. The best-characterized examples involve transport of animal viruses by microtubules to the nucleus or other intracellular destinations. In plant viruses, most work to date has focused on interaction between viral movement proteins and the cytoskeleton, which is t...
Du fait de l'immobilité de leurs hôtes, l'immense majorité des virus de plante utilisent des vecteurs spécifiques pour passer d'un hôte à un autre. Ces "véhicules de transport" sont principalement des arthropodes et en grande majorité des pucerons, qui sont des insectes de type piqueur-suceur. Pour les interactions virus-vecteur, la stratégie la pl...
Auteur(s) : M Blanc1, M Uzest1, T Candresse2, M Drucker1, A Fereres3, D Gargani1, E Garzo3, E Hebrard4 1UMR BGPI, Inra-Cirad-AgroM, TA A54/K, Campus international de Baillarguet, 34398 Montpellier Cedex 05 2Inra, UMR Genomique, Villenave d’Ornon 3CSIC, CCMA, Madrid, Espagne 4IRD, Montpellier Du fait de l’immobilite de leurs hotes, l’immense majorit...
Hundreds of species of plant viruses, many of them economically important, are transmitted by noncirculative vector transmission (acquisition by attachment of virions to vector mouthparts and inoculation by subsequent release), but virus receptors within the vector remain elusive. Here we report evidence for the existence, precise location, and che...
Cauliflower mosaic virus (CaMV) is transmitted by aphids. For acquisition by the vector, a transmissible complex must form, composed of the virus particle, the viral coat-associated protein P3 and the helper protein P2. However, the components of the transmissible complex are largely separated in infected plant cells: most P3 virions are confined i...