Martijn Schuijs

Martijn Schuijs
Vlaams Instituut voor Biotechnologie | VIB · Inflammation Research Center (IRC)

PhD

About

48
Publications
8,537
Reads
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1,745
Citations
Additional affiliations
October 2017 - present
Cancer Research UK Cambridge Institute
Position
  • PostDoc Position
October 2011 - September 2017
Vlaams Instituut voor Biotechnologie
Position
  • PhD Student
October 2011 - September 2017
Ghent University
Position
  • PhD Student

Publications

Publications (48)
Article
Full-text available
Senescence is a stress-responsive tumor suppressor mechanism associated with expression of the senescence-associated secretory phenotype (SASP). Through the SASP, senescent cells trigger their own immune-mediated elimination, which if evaded leads to tumorigenesis. Senescent parenchymal cells are separated from circulating immunocytes by the endoth...
Article
Full-text available
The prevalence of allergic disease has increased significantly over the past decades. Although allergies are inherently multifactorial and heterogenous; environmental, maternal, and early-life microbial exposures could strongly modify disease risk. The effects of environmental microbiota are illustrated by the "farm effect", showing protection agai...
Article
Full-text available
An elevated neutrophil–lymphocyte ratio negatively predicts the outcome of patients with cancer and is associated with cachexia, the terminal wasting syndrome. Here, using murine model systems of colorectal and pancreatic cancer we show that neutrophilia in the circulation and multiple organs, accompanied by extramedullary hematopoiesis, is an earl...
Article
Full-text available
Eosinophils are typically a minority population of circulating granulocytes being released from the bone-marrow as terminally differentiated cells. Besides their function in the defense against parasites and in promoting allergic airway inflammation, regulatory functions have now been attributed to eosinophils in various organs. Although eosinophil...
Preprint
Full-text available
An elevated neutrophil-to-lymphocyte ratio negatively predicts the outcome of patients with cancer and is associated with cachexia, the terminal wasting syndrome. Here, we show using murine model systems of colorectal and pancreatic cancer that neutrophilia in the circulation and multiple organs, accompanied by extramedullary hematopoiesis, is an e...
Article
Full-text available
Regulation of epithelial cell death has emerged as a key mechanism controlling immune homeostasis in barrier surfaces. Necroptosis is a type of regulated necrotic cell death induced by receptor interacting protein kinase 3 (RIPK3) that has been shown to cause inflammatory pathologies in different tissues. The role of regulated cell death and partic...
Article
Full-text available
Metastasis constitutes the primary cause of cancer-related deaths, with the lung being a commonly affected organ. We found that activation of lung-resident group 2 innate lymphoid cells (ILC2s) orchestrated suppression of natural killer (NK) cell-mediated innate antitumor immunity, leading to increased lung metastases and mortality. Using multiple...
Article
Rationale: Emerging evidence supports a crucial role for tertiary lymphoid organs (TLOs) in chronic obstructive pulmonary disease (COPD) progression. However, mechanisms of immune cell activation leading to TLO in COPD remain to be defined. Objectives: To examine the role of lung dendritic cells (DC) in T follicular helper (Tfh)-cell induction, a T...
Article
Full-text available
MeDALL (Mechanisms of the Development of ALLergy; EU FP7-CP-IP; Project No: 261357; 2010-2015) has proposed an innovative approach to develop early indicators for the prediction, diagnosis, prevention and targets for therapy. MeDALL has linked epidemiological, clinical and basic research using a stepwise, large-scale and integrative approach: MeDAL...
Article
Full-text available
Background The emergence of IL-33 as a key molecular player in the development and propagation of widespread inflammatory diseases, including asthma and atopic dermatitis, has established the need for effective IL-33–neutralizing biologics. Objective Here we describe the development and validation of a new antagonist of IL-33, termed IL-33trap, wh...
Article
Dendritic cells (DCs) are critical for the activation of naïve CD4⁺ T cells and are considered professional antigen-presenting cells (APCs), as are macrophages and B cells. Recently, several innate type 2 immune cells, such as basophils, mast cells (MCs), eosinophils, and innate type 2 lymphocytes (ILC2), have also emerged as harboring APC behavior...
Article
Full-text available
Chronic helminth infection with Schistosoma (S.) mansoni protects against allergic airway inflammation (AAI) in mice and is associated with reduced Th2 responses to inhaled allergens in humans, despite the presence of schistosome‐specific Th2 immunity. Schistosome eggs strongly induce type 2 immunity and allow to study the dynamics of Th2 versus re...
Chapter
The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-cu...
Article
Group 2 innate lymphoid cells (ILC2) are innate immune cells that respond rapidly to their environment through soluble inflammatory mediators and cell-to-cell interactions. As tissue-resident sentinels, ILC2 help orchestrate localized type-2 immune responses. These ILC2-driven type-2 responses are now recognized both in diverse immune-processes, di...
Article
Full-text available
Group 2 innate lymphoid cells (ILC2) are innate immune cells that respond rapidly to their environment through soluble inflammatory mediators and cell-to-cell interactions. As tissue-resident sentinels, ILC2 help orchestrate localized type 2 immune responses. These ILC2-driven type 2 responses are now recognized in diverse immune processes, differe...
Article
Full-text available
Background: It is currently unknown, why allergen exposure or environmental triggers in mild to moderate asthma patients results in Th2-mediated eosinophilic inflammation, whereas severe asthma patients often present with Th17-mediated neutrophilic inflammation. The activation state of dendritic cells (DCs) is crucial for both Th2 and Th17-cell di...
Article
Asthma, rhinitis, and eczema are complex diseases with multiple genetic and environmental factors interlinked through IgE-associated and non–IgE-associated mechanisms. Mechanisms of the Development of ALLergy (MeDALL; EU FP7-CP-IP; project no: 261357; 2010-2015) studied the complex links of allergic diseases at the clinical and mechanistic levels b...
Article
Allergic disease originates in early life and polymorphisms in interleukin-33 gene (IL33) and IL1RL1, coding for IL-33R and decoy receptor sST2, confer allergy risk. Early life T helper 2 (Th2) cell skewing and allergy susceptibility are often seen as remnants of feto-maternal symbiosis. Here we report that shortly after birth, innate lymphoid type...
Article
Background: Allergic asthma is a CD4 Th2-lymphocyte driven disease characterized by airway hyper-responsiveness and eosinophilia. B cells can present antigens to CD4 T cells and produce IgE immunoglobulins which arms effector cells, however mouse models are inconclusive whether B cells are necessary for asthma development. Objectives: To address...
Article
Full-text available
Respiratory Syncytial Virus (RSV) is a major pathogen causing low respiratory tract disease (bronchiolitis), primarily in infants. Helminthic infections may alter host immune responses to both helminths and to unrelated immune triggers. For example, we have previously shown that filarial cystatin (AvCystatin/Av17) ameliorates allergic airway inflam...
Data
Reduced RSV-induced immunopathology by AvCystatin treatment in a model of FI-RSV of viral lung eosinophilia. A) Schematic of the FI RSV model: i.m. intramuscular; i.p. intraperitoneal; i.n. intranasal application. B) Weight loss in the FI-RSV model. Total cell number of eosinophil (C) and macrophages in the BAL (D). Viral load in the lungs measured...
Data
Frequency of IL-10 producing T-cells in the lungs and airways after AvCystatin treatment. Flowcytometric analysis of IL-10 intracellular cytokine content in RSV challenged or AvCystatin/RSV challenged mice is shown (A). Graphical visualization of IFNγ and IL-10 production by CD4+ T cells in the BAL (D and E) and lungs (B and C). Representative data...
Data
AvCystatin induced FoxP3+ T cell induction in the mediastinal lymph node. Total number of FoxP3+ CD4+ T cells (A) and the number of IL-10+ CD4+ T cells in the mLN (B) after AvCystatin treatment and RSV challenge. Representative data of 2 experiments, 5 mice per group. Error bars indicate SEM. P values reflect Mann-Whitney t-test: * p<0.05, **p<0.01...
Data
AvCystatin treatment reduces Muc5a production in lung. Relative expression of MUC5a in mice lungs after the vvG RSV model (A) or primary RSV model (B). Error bars indicate SEM. P values reflect Mann-Whitney t-test: * p<0.05. (TIF)
Article
MeDALL Mechanisms of the Development of ALLergy; EU FP7-CP-IP; Project No: 261357; 20 (10) -2015 proposed an innovative approach to develop early indicators for the prediction, diagnosis, prevention and targets for therapy(.) MeDALL linked epidemiological, clinical and basic research using a stepwise, large-scale and integrative approach: precisely...
Article
Full-text available
A20 negatively regulates multiple inflammatory signalling pathways. We here addressed the role of A20 in club cells (also known as Clara cells) of the bronchial epithelium in their response to influenza A virus infection. Club cells provide a niche for influenza virus replication, but little is known about the functions of these cells in antiviral...
Data
Influenza A X-47 infects CCSP expressing club cells and IFNα expression after X-47 challenge. (A) Immunohistochemical stain for CCSP on lung sections of wild type mice (upper scale bar 50μm, lower scale bar 20μm). (B) Immunohistochemical stain for influenza A M2 protein on wild type mice mock (left) or X-47 virus infected (right) analyzed 4 days (4...
Data
DC kinetics in MLN and peripheral adaptive immune responses are not altered in A20AEC-KO mice. (A) Absolute numbers of CD11b-, CD11b+ and inflammatory DCs (iDC) in mediastinal lymph nodes (MLN) measured by flow cytometry at 2, 5, 8 and 12 days post-infection (days p.i.) after challenge with 0.05 X LD50 X-47. (B) Cells isolated from lungs or spleens...
Data
Deficiency of A20 in club cells protects against PR8 IAV infection. Weight loss of A20AEC-KO (n = 6) or wild type littermates (A20WT, n = 9) monitored until 14 days post infection (days p.i.) with a sublethal dose of the A/Puerto Rico/8/34 (PR8) strain (0.17 X LD50). Data were analysed using 2-way ANOVA (*p < 0.05) and are shown as mean ± SEM. (TIF...
Data
Lung histology and IFNα expression after X-47 challenge. (A) Representative pictures from hematoxylin and eosin stained lung tissue sections from A20AEC-KO and control wild-type (WT) littermate mice at different times p.i. Scale bar, 100 μm. (B) IFNα, IFNβ, and CXCL10 protein levels in BAL fluid of A20AEC-KO mice and control littermates as determin...
Data
Influenza A X-47 infection does not significantly increase club cell apoptosis in A20AEC-KO mice. (A) DEVDase assay to quantify caspase-3 activity in tissue homogenates of lungs of A20AEC-KO and control littermates (A20WT) at different days post infection (days p.i.) with 0.05 X LD50 of X47 virus. Data are shown as mean ± SEM of at least 4 mice per...
Data
Reduced CCL2 expression in CCSP expressing club cells from A20AEC-KO mice after influenza A virus infection. (A) CCL2 (MCP-1), KC (CXCL1) and Rantes (CCL5) protein levels in BAL fluid measured by Multiplex immunoassay at indicated time points post-infection. (B) Immunohistological section of inflamed A20WT and A20AEC-KO lung issue stained with anti...
Data
Analysis of A20 (Tnfaip3) deletion in club cells of A20AEC-KO mice. (A) Scheme of conditional A20 gene targeting. Boxes indicate exons 1–9 (E1-E9; black boxes depict coding sequence). E4 and E5 are flanked by 2 LoxP sites (arrowhead). Restriction enzymes (B, BamHI and V, EcoRV) and the location of the probe for Southern Blot analysis are also annot...
Article
Although the field of cancer immunotherapy is intensively investigated, there is still a need for generic strategies that allow easy, mild and efficient formulation of vaccine antigens. Here we report on a generic polymer-protein ligation strategy to formulate protein antigens into reversible polymeric conjugates for enhanced uptake by DCs and pres...
Article
Full-text available
Nanomaterials hold potential of altering the interaction between therapeutic molecules and target cells or tissues. High aspect ratio nanomaterials in particular have been reported to possess unprecedented properties and are intensively investigated for their interaction with biological systems. Grahene oxide (GOx) is a water-soluble graphene deriv...
Article
Full-text available
Growing up on a dairy farm protects children from allergy, hay fever, and asthma. A mechanism linking exposure to this endotoxin (bacterial lipopolysaccharide)-rich environment with protection has remained elusive. Here we show that chronic exposure to low-dose endotoxin or farm dust protects mice from developing house dust mite (HDM)-induced asthm...
Article
Full-text available
Asthma is a T helper 2 (Th2)-cell-mediated disease; however, recent findings implicate Th17 and innate lymphoid cells also in regulating airway inflammation. Herein, we have demonstrated profound interleukin-21 (IL-21) production after house dust mite (HDM)-driven asthma by using T cell receptor (TCR) transgenic mice reactive to Dermatophagoides pt...
Article
Full-text available
Foxp3(+) regulatory T cells (Tregs) maintain immune homoeostasis through mechanisms that remain incompletely defined. Here by two-photon (2P) imaging, we examine the cellular dynamics of endogenous Tregs. Tregs are identified as two non-overlapping populations in the T-zone and follicular regions of the lymph node (LN). In the T-zone, Tregs migrate...
Article
Full-text available
Interleukin (IL-) 10 is a pleiotropic cytokine with broad immunosuppressive functions, particularly at mucosal sites such as the intestine and lung. Here we demonstrate that infection of BALB/c mice with respiratory syncytial virus (RSV) induced IL-10 production by CD4(+) and CD8(+) T cells in the airways at later time points (e.g. day 8); a propor...

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