Marlene Jara

Marlene Jara

PhD
Molecular parasitology

About

17
Publications
2,755
Reads
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280
Citations
Additional affiliations
October 2019 - October 2022
Research Foundation Flanders
Position
  • Postdoctoral research fellow
April 2019 - June 2019
Institute of Tropical Medicine of Antwerp
Position
  • Research fellow
April 2015 - March 2019
Institute of Tropical Medicine of Antwerp
Position
  • PhD (c) fellow
Education
January 2015 - March 2019
University of Antwerp
Field of study
  • Biomedical Science

Publications

Publications (17)
Article
Full-text available
Earlier histopathology studies suggest that parasite loads may differ between cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML) lesions and between acute and chronic CL. Formal demonstration requires highly sensitive detection and accurate quantification of Leishmania in human lesional tissue. In this study, we developed a quantitative re...
Article
Full-text available
It was recently hypothesized that Leishmania amastigotes could constitute a semi-quiescent stage characterized by low replication and reduced metabolic activity. This concept developed with Leishmania (Leishmania) mexicana and Leishmania (Leishmania) major models might explain numerous clinical and sub-clinical features of Leishmania (Viannia) braz...
Article
Full-text available
Under stressful conditions some microorganisms adopt a quiescent stage characterized by a reversible non or slow proliferative condition that allows their survival. This adaptation was only recently discovered in Leishmania. We developed an in vitro model and a biosensor to track quiescence at population and single cell levels. The biosensor is a G...
Article
Full-text available
Here, we report a pilot study paving the way for further single cell genomics studies in Leishmania. First, the performances of two commercially available kits for Whole Genome Amplification (WGA), PicoPLEX and RepliG were compared on small amounts of Leishmania donovani DNA, testing their ability to preserve specific genetic variations, including...
Article
Full-text available
Microorganisms can adopt a quiescent physiological condition which acts as a survival strategy under unfavorable conditions. Quiescent cells are characterized by slow or non-proliferation and a deep downregulation of processes related to biosynthesis. Although quiescence has been described mostly in bacteria, this survival skill is widespread, incl...
Conference Paper
Full-text available
Resistance against the currently available Leishmanicidal drugs has been studied for years, giving important insights into how Leishmania adapt to these drugs and select heritable traits that make a new parasite population unsusceptible to these drugs. However, Leishmania can also survive lethal drug exposure without heritable genetic resistance, a...
Article
Full-text available
American Tegumentary Leishmaniasis (ATL) is an endemic and neglected disease of South America. Here, mucosal leishmaniasis (ML) disproportionately affects up to 20% of subjects with current or previous localised cutaneous leishmaniasis (LCL). Preclinical and clinical reports have implicated the Leishmania RNA virus-1 (LRV1) as a possible determinan...
Article
Full-text available
Humans and their pathogens are continuously locked in a molecular arms race during which the eventual emergence of pathogen drug resistance (DR) seems inevitable. For neglected tropical diseases (NTDs), DR is generally studied retrospectively once it has already been established in clinical settings.
Article
Full-text available
Significance Parasites are interesting models for studying speciation processes because they have a high potential for specialization, thanks to the intimate ecological association with their hosts and vectors. Yet little is known about the circumstances under which new parasite lineages emerge. Here we studied the genome diversity of parasites of...
Chapter
A pathogen’s fitness relates to all biological processes that ensure its survival, reproduction, and transmission in specific conditions. These often include the presence of drugs, forcing pathogens to adapt and develop drug resistance in order to survive. The acquisition of a drug-resistant trait usually comes at a cost, making drug-resistant para...
Article
Full-text available
Mucosal leishmaniasis (ML) is a disfiguring manifestation of Leishmania (Viannia) infection. We evaluated parasite load (PL) over time as a potential biomarker of treatment outcome in ML. PL was assessed with kinetoplast DNA quantitative real-time polymerase chain reaction (kDNA-qPCR) at enrollment, days 14 and 21–28 of therapy and 3, 6, 12–18, and...
Article
Full-text available
Cutaneous leishmaniasis (CL) is a skin disease caused by the protozoan parasite Leishmania. Few studies have assessed the influence of the sample collection site within the ulcer and the sampling method on the sensitivity of parasitological and molecular diagnostic techniques for CL. Sensitivity of the technique can be dependent upon the load and d...
Conference Paper
Full-text available
Background: American tegumentary leishmaniasis (ATL) is a neglected disease of South America where metastatic mucosal lesions (ML) can be seen in up to 5-20% of Leishmania Viannia complex infections. The Leishmania RNA virus-1 (LRV1) has been recently implicated as a possible pathogenic agent in ATL since disseminated disease (DisL) was induced by...
Conference Paper
Full-text available
Leishmania parasites are ancestral eukaryotes with unusual characteristics like polycistronic transcription and RNA trans-splicing. Like other eukaryotes, their RNA ribosomal genes are tandemly repeated and transcribed by RNA polymerase I. Unlike other eukaryotes, Leishmania ribosomes have rRNA molecules of 18S, 5.8S, and 28S, with the latter one b...

Questions

Question (1)
Question
Hello, I am doing a western blot using crude extract of leismania for the detection of a basic protein by western blot. The expected size is 17.5 but on my western blot i have a very good band but with a MW of about 14.7. 
Any ideas?
Thank you very much

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Projects

Project (1)
Project
We aim to i) implement models of quiescence in vitro for their further characterization at transcriptomic and metabolomic levels, ii) integrate both omics layers to identify the most essential molecular and metabolic processes for the maintenance of quiescence.