Marko Pajić

Scientific Veterinary Institute "Novi Sad" · Department for Epizootiology, Clinical Diagnostic, Pathology and DDD

In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory identification and pathology associated with highly pathogenic avia...

The objective was to assess the dairy cows' welfare quality in relation to the housing system: loose (LHS) vs. tie-stall (THS). A survey was done according to the Welfare Quality® Protocol for Cattle on 16 dairy farms (7 in LHS and 9 in THS) in Serbia. From a total number of 4833 dairy cows (2392 cows in THS and 2441 cows in LHS) 551 cows in THS an...

Infectious laryngotracheitis (ILT) is a respiratory disease of poultry characterized by high morbidity and variable mortality. ILT is caused by Gallid alpha herpesvirus-1 (GaHV-1), which is transmitted horizontally and most susceptible are chickens older than 4 weeks. After almost two decades since last appearance of this disease in Vojvodina, an o...

Background The Sarconema eurycerca heart infection may be one of the main causes of death in swans and geese due to severe heart lesions. To date, there have been no reports of Sarconema eurycerca in mute swans (Cygnus olor) in Serbia and this parasite has never been morphologically characterized in wild birds from this region.Methods In spring 202...

This experiment was performed to investigate the protective effect of a new combined adsorbent (bentonite + yeast cell wall extract) previously tested in vitro, on the occurrence of pathological lesions in broilers fed a diet contaminated with aflatoxin B1 until 21 days of age. A total of 96 one-day old Ross 308 hybrid broiler chickens were divided...

The first confirmed outbreak of highly pathogenic avian influenza virus (HPAI) H5N8 in birds was documented in Serbia in winter 2016/2017. Afterwards, new disease outbreaks were reported between March and May 2021. The aim of this study was the evaluation of macroscopic lesions and virological findings in mute swans infected naturally with HPAI H5N...

Harmful gases, such as ammonia (NH3) and carbon dioxide (CO2), affect both birds and workers involved in broiler production. Five hundred and seventy-six one-day-old ROSS 308 broilers were reared on six types of litter (Treatment 1: chopped wheat straw; treatment 2: wood shavings; treatment 3: mixture of 1/3 chopped wheat straw, 1/3 wood shavings a...

The aim of this study was to determine the effect of housing system and the age of hens on welfare parameters and the prevalence of keel bone damage in laying hens. In this study two housing systems were evaluated: aviary system and enriched cages. From each system and age, we used 50 randomly selected hens from different cages and tiers. The resul...

In intensive commercial broiler production, broiler chickens are raised under a deep litter system having an absorbent role. The broiler production has over the years rapidly expanded with increasing concern of poultry waste disposal and harmful gases emission. Bedding material plays a significant role in poultry rearing. Harmful gases in broiler p...

The experiment was conducted to evaluate the effect of alternative bedding materials on broiler production performances and litter quality in plant production. A 40-day experiment was carried out on 6885 broilers of ROSS 308 provenience. The broilers were reared on bedding material which consisted of cellulose pellets, wood chips, peat moss, and pH...

In the Northern part of Serbia, which is part of Pannonian Basin, mute swan (Cygnus olor) population has notably increased over the last few decades. Like other birds from Anatidae family, mute swans are a host of numerous endoparasite species. Th e aim of this study was to acquire the data on identifi cation and prevalence of endoparasites in mute...

The contamination of animal feed with mycotoxins is a worldwide problem in the animal husbandry, but it also represents a serious threat for the whole food chain. The health of both animals and humans is potentially endangered. From this point of view aflatoxins are a class of mycotoxins especially well known. Therefore, new strategies to combat th...

Međunarodna trgovina zahteva bezbednu hranu zasnovanu na posebnim higijenskim standardima, transpartentnim procedurama i programima. Meso, zbog visokog sadržaja vode i hranljivih materija, predstavlja idealnu sredinu za rast i razmnožavanje mikroorganizama. Salmonella, kao jedan od najčešćih patogena, koji se putem hrane prenose sa životinja na lju...

International trade requires food safety guarantees based on specialized hygiene standards, transparency procedures, and programs. Meat, because of its high water content and nutrition, can be an ideal medium for microorganism growth and multiplication. Salmonella, as one of the most common pathogens that can be transmitted from animals to humans,...

In this study, antibiotic resistance and virulence genes and clonal relationships among Escherichia coli isolates from poultry litter and healthy poultry were investigated on a farm with previous outbreak of avian pathogenic E. coli (APEC) colisepsis, in order to establish a system for evaluating management practice and antibiotic use on poultry fa...

Necrotic enteritis (NE) or poultry clostridiosis is a disease which poses enormous health problems and makes tremendous economic losses to intensive poultry production worldwide. Despite having been targeted in extensive research for decades, a number of aspects of its pathogenesis remain unknown. For more than 30 years alfa-toxin has been consider...

In the study conducted during the two-year period (2017 - 2018) the cause of death of certain protected and strictly protected wild bird species from the territory of Vojvodina was analysed. During this period, 57 wild birds were brought for post-mortem examination (a total of 9 protected species) to Scientific veterinary institute "Novi Sad". A de...

Lumpy skin disease (LSD) presents constant threat to cattle production because of the high economic losses it causes. Although a lot has been done in the field of improving serological diagnostics of LSD, a reliable determination of immunity status on both herd and individual level remains problematic. In order to test the efficacy of vaccination a...

The aim of this study was to compare pathological lesions and viral antigen expression in the organs of mute swans (Cygnus olor) naturally infected with highly pathogenic avian influenza virus subtypes H5N1 and H5N8. The examination was conducted on the carcasses of 22 mute swans which died during the avian influenza outbreaks in Serbia in 2006 and...

The influence of certain anticoccidial drugs on oxidative stress in broiler chickens infected with Eimeria species was assessed. There were two untreated (uninfected and infected), and three groups infected and treated with anticoccidials. The first treated group (Ro) was given robenidine, the 2 nd a herbal anticoccidial (Herb) and the 3 rd the com...

The results of the research into the microbiological contamination of litter used by broiler chickens are presented. Litter samples were taken prior to the introduction of chicks (day 0) and in 7-day intervals until the end of the fattening period. The total numbers of aerobic mesophilic microorganisms, yeasts, moulds and Clostridium perfringens sp...

Cases of deliberate, illegal animal poisoning are widely documented in the literature. Recently, there has been an increase in number of cases of poisoning of domestic and wild animals with highly toxic pesticides in the Republic of Serbia. During the two-year period (2016-2017), in total 40 autopsies of dogs and 2 cats from the territory of the So...

Cases of deliberate, illegal animal poisoning are widely documented in the literature. Recently, there has been an increase in number of cases of poisoning of domestic and wild animals with highly toxic pesticides in the Republic of Serbia. During the two-year period (2016-2017), in total 40 autopsies of dogs and 2 cats from the territory of the So...

Поједини високо патогени сојеви авијарне инфлуенце попут H5N1 имају изражен тропизам према нервном ткиву. Неуротропизам је заједничка карактеристика како експерименталних тако и природних инфекција изазваних вирусом авијарне инфлуенце подтипа H5N1 код различитих врста птица, а такође и појединих сисара укључујући и човека. Током епизоотије високо п...

Mycoplasma synoviae is known to cause respiratory disorders, syno-vitis, subclinical infections, air sacculitis and eggshell apex abnormalities in domestic poultry worldwide. Th e aim of this study was to determine M. synoviae seroprevalence in 5 diff erent broiler breeder farms in South Bačka from 2014 to 2017. A total of 1511 samples were tested...

Labudovi su divlje ptice vodenih staništa i pripadaju familiji Anatidae, roduAnseriformes. S obzirom na stanište, način života i ishranu, labudovi predstavljajuznačajnu bioindikatorsku vrstu za monitoring zagađivača u prirodi. Hrane seuglavnom vodenim biljkama i zelenim algama koje predstavljaju direktan pokazateljzagađenja životne sredine i vodeni...

Eimeria türleri ile enfekte Broilerlerde bazı antikoksidiyal ilaçların oksidatif stress üzerine etkisi araştırılmıştır. Çalışmada, enfekte edilmeyen ve enfekte edilen olmak üzere iki, ayrıca enfekte edilerek uygulama yapılan üç grup vardı. Uygulama yapılan gruplardan ilkine robenidin (Ro), ikincisine bitkisel antikoksidiyal (Herb) ve üçüncüsüne rob...

Mycoplasma synoviae is known to cause respiratory disorders, synovitis, subclinical infections, air sacculitis and eggshell apex abnormalities in domestic poultry worldwide. Th e aim of this study was to determine M.synoviae seroprevalence in 5 diff erent broiler breeder farms in South Bačka from 2014 to 2017. A total of 1511 samples were tested us...

Dermanyssus gallinae or the poultry red mite is currently the most im-portant ectoparasite aff ecting egg-laying hens in several countries causing reduced poultry welfare, mortality and even allergic reactions in poultry farms workers. Its short life cycle, which in optimal conditions can be com-pleted within 7 days, and ability to survive in extre...

Salmonellosis represent, after campylobacteriosis, the most common foodborne disease. The principal serotypes are Salmonella enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica serovar Typhimurium (S. Typhimurium), but the incidence of human illness caused by serotypes S. Infantis, S. Mbandaka and others, are in constant increase....

Od 2014 godine nekoliko epizootija izazvanih visoko patogenim virusom avijarne infl uence podtipa H5N8, izbilo je u Aziji, Evropi i severnoj Americi. Ovaj tip virusa infl uence uzrokovao je infekcije domaće živine i divljih ptica. Globalnom širenju virusa doprinele su intrakontinentalne i interkontinentalne migracije ptica. Prvi slučaj visoko patog...

Dermanyssus gallinae or the poultry red mite is currently the most im-portant ectoparasite aff ecting egg-laying hens in several countries causing reduced poultry welfare, mortality and even allergic reactions in poultry farms workers. Its short life cycle, which in optimal conditions can be com-pleted within 7 days, and ability to survive in extre...

Since early 2014 several outbreaks have occurred in Asia, Europe and North America involving novel highly pathogenic avian influenza subtype A(H5N8) viruses. This type of avian influenza virus have infected poultry and wild bird species. Global spread of these viruses was attributed to intracontinental and intercontinental movement of migratory bir...

The two corpses of police dogs, of Labrador retriever breed used for fi nding narcotics, were sent to the Scientifi c Veterinary Institute “Novi Sad”, and examined post-mortem, in order to determine the cause of death. Post-mortem examination revealed the presence of neoplastic changes in the mandibular region, pulmonary parenchyma and axillary reg...

The objectives of the present paper are the analysis and comparison of the results from available literature regarding the prevalence of tetracycline resistance in Escherichia coli strains isolated from poultry in different countries. Tetracycline is still one of the most commonly used antibiotic in many developing countries both in human and in ve...

Introduction: Novel molecular techniques applied in biotechnology research have provided sound evidence on clonal persistence of distinct serovars of Salmonella in feed factory environments, over long periods of time (months, even years), which can be responsible for repeated in-house contamination of final products. In this study, we examined the...

The relationship between the use of antibiotics and the presence of antibiotic resistant strains of E. coli , as well as antibiotic residues in poultry litter, mainly due to irregular use of antibiotics is reviewed in the present paper. The aim of this review is to examine the existing problem of poultry waste management and how it can impact agric...

Salmonella causes local and systemic infections of poultry, which may lead to substantial direct and indirect economic losses, presenting also signifi cant risk to human health. Th e aim of this study was to monitor the occurrence of certain serotypes of Salmonella spp. isolated on poultry farms in Southern Bačka and Srem regions in the period from...

The resistance of Escherichia coli (E. coli) to fluoroquinolones has become a serious issue on large pig farms worldwide, since these antimicrobial agents are widely used in the control of various diseases such as neonatal diarrhea, post weaning diarrhea, the edema disease as well as others. Being a frequent inhabitant of the digestive tract, E.col...

Th e results of our 15-year long research on the eff ects of global warming in our region that relies on the database obtained from the Republic Hydrometeorological Service of the Republic of Serbia strongly suggested changes in the pattern of disease events associated with inevitable occurrence of heat stress in poultry regardless of the species a...

Control of Salmonella in primary production of broiler chickens Pajić Marko, Karabasil Neđeljko, Todorović Dalibor, Milanov Dubravka, Dmitrić Marko, Lakićević Brankica, Đorđević Vesna Salmonellae represent one of the main causes of zoonotic diseases in people, triggered by ingestion of contaminated food, mostly poultry meat and eggs. The presence o...

Abstract The fungi of the genus Aspegillus are ubiquitous saprophytic microorganisms which are, in certain circumstances, responsible for clinical infections of respiratory tract in all poultry, particularly in young birds. Aspergillus fumigatus, A. niger and A. glaucus are the most common isolated fungi in the cases of pulmonary aspergillosis in p...

Salmonella causes local and systemic infections of poultry, which may lead to substantial direct and indirect economic losses, presenting also significant risk to human health. The aim of this study was to monitor the occurrence of certain serotypes of Salmonella spp. isolated on poultry farms in Southern Bačka and Srem regions in the period from 2...

Bacteria of the genus Salmonella, some Escherichia coli strains as well as other species of the family Enterobacteriaceae, manifest the ability to express a specific phenotype, which is commonly termed as the 'rdar' (red, dry and rough) morphotype. Both rdar and non-rdar phenotypes are commonly found in the nature; however, rdar is of essential imp...