Marko Geric

Marko Geric
Institute for Medical Research and Occupational Health · Mutagenesis Unit

Master of Science

About

70
Publications
9,291
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
729
Citations

Publications

Publications (70)
Conference Paper
Full-text available
Outdoor air pollution in urban and rural areas causes millions of premature deaths worldwide. Cities are more exposed to higher concentrations of pollutants because of the urban agglomeration, traffic, and industrial activities. Additionally, the emission of BTEX (benzene, toluene, ethylbenzene, and xylene) compounds becomes higher, mainly due to i...
Chapter
Human biomonitoring is an important approach that enables the assessment of whether and to what extent environmental agents impact human population, thus facilitating the identification of health risks by providing data on environmental exposure. One of the advantages of human biomonitoring is that it can provide very accurate information about an...
Article
Full-text available
Aims: The literature highlights the pathology of inflammation and its role in carcinogenesis, aging, and related diseases. Inflammatory processes induce oxidative stress and reduce antioxidant capacity. This study investigated the antioxidant and anti-inflammatory potential of probiotic bacteria isolated from fermented whey under conditions of ind...
Article
Domoic acid (DA) is a marine neurotoxin produced as a defence compound by diatom Pseudo-nitzschia. Although its toxicity is well known in marine mammals and fish, data on DA cyto/genotoxicity in human non-target cells is still limited. Hence, we aimed to study the effect of DA (0.001 to 10 µg/mL) on cell viability and proliferation kinetics of huma...
Article
Aging-related impaired body structure and functions may be, at least partially, caused by elevated oxidative stress. Melatonin (MEL) and resveratrol (RSV) may act as antioxidant and anti-aging compounds, but these actions in experimental animals and humans are controversial. Herein, a rat model of aging was used to study the long-term sex-related e...
Article
The health benefit of a vegetarian diet is still under debate as it may result in a higher intake of some beneficial micronutrients, while others may be reduced, thus influencing various metabolic pathways and health-related biomarkers. This scoping review discusses inflammatory, oxidative and DNA damage status in vegetarians and vegans compared to...
Conference Paper
Full-text available
Air pollution is becoming recognised as one of the most serious public health issues worldwide. Rising industrial and energy productions, the burning of fossil fuels and biomass, as well as the rise in road traffic frequency contribute to air pollution in our cities. Urban air is a complex and variable mixture of many different chemicals whose exac...
Conference Paper
Full-text available
Major world authorities such as European Environmental Agency, World Health Organization, International Agency for Research on Cancer, and The Global Burden of Diseases highlighted air pollution as leading global health threat responsible for 3 – 7 million deaths annually. Urban air is a variable and complex mixture of many different chemicals with...
Article
Polyoxo-noble-metalates (PONMs), a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of next-generation antitumor metallodrugs. This study aimed to evaluate the antitumor potential against human neuroblastoma cells (SH-SY5Y), as well as toxic...
Article
Full-text available
Cardiotoxicity is a frequent undesirable phenomenon observed during oncological treatment that limits the therapeutic dose of antitumor drugs and thus may decrease the effectiveness of cancer eradication. Almost all antitumor drugs exhibit toxic properties towards cardiac muscle. One of the underlying causes of cardiotoxicity is the stimulation of...
Article
Traditionally, equid milk has been used as an alternative to human milk for infants or children mainly because of its similarities in nutrient composition, hypoallergenicity, immune modulation and antimicrobial activity. As a highly nutritious substrate, it possesses great potential for probiotic bacteria isolation. The aim of this study was to ass...
Article
Imatinib mesylate (IM) is an anticancer drug that belongs to tyrosine kinase inhibitors. We report the results of the first investigation of the chronic exposure of zebrafish (Danio rerio) to IM. The exposure to IM (0.01, 1 and 100 μg/L) was initiated in adult fish and continued through hatching and the offspring generation for seven months. In add...
Article
Domoic acid (DA) is an excitatory marine neurotoxin produced by diatoms Pseudo-nitzschia spp. as a defence compound that accumulates in the food web and is associated with amnesic shellfish poisoning in humans. Although its toxicity has been well established in marine species, there is limited data on DA cytogenotoxicity in human non-target cells....
Article
This study aimed to evaluate occupational exposure to a styrene and xylene mixture through environmental exposure assessment and identify the potential genotoxic effects through biological monitoring. Secondly, we also exposed human peripheral blood cells in vitro to both xylene and styrene either alone or in mixture at concentrations found in occu...
Article
Purpose Diagnostic X-ray examinations of paranasal sinuses use a low-dose ionizing radiation to achieve medically indicated purposes. The effects of low-dose radiation are still controversial, making it a highly prioritized field of research. As there is a need to evaluate the effects of low-dose ionizing radiation and that children might be a more...
Article
Objectives The beneficial influence of a vegetarian diet in reducing the risk of metabolic syndrome has been evidenced. However, adiponectin production and secretion are scarcely studied in vegetarians, despite the important role in recovering metabolic homeostasis by reducing visceral obesity, inflammation, and insulin resistance. This study aimed...
Chapter
Securing potable water and preserving aquatic ecosystems are growing problems that will become even more important in the near future. Well-defined environmental pollutants such as pesticides, heavy metals, or organic matter have been thoroughly examined, but over the recent decade, a spotlight was put on a new and emerging group of pollutants – ph...
Article
An adequate level of low molecular weight thiols (LMW-SH, especially glutathione (GSH)) protects cellular macromolecules against toxic agents, and is used as a sensitive biomarker of exposure to toxic compounds. During sample collection, storage and preparation, non-enzymatic and enzymatic oxidation of LMW-SH can occur leading to analytical inaccur...
Poster
Beneficial health effects of lactic acid bacteria (LAB) used in functional foods and pharmaceutical products are based on the ability and capacity of these microorganisms in stimulation of host immune system. Health improving immune modulation and production of bioactive substances are restricted only to some LAB strains. In this study, peripheral...
Article
This study proposes the application of the comet assay for the evaluation of DNA damage from frozen human whole blood samples that could be readily used in human biomonitoring and epidemiological studies. It was done on simply frozen whole blood samples collected from male volunteers (N = 60) aliquoted in small volumes and stored at -80 °C without...
Article
Imatinib mesylate (IM) is the first developed protein kinase inhibitor and recently it has topped consumption rates among targeted and total anticancer drugs. Although there are indications that IM possesses cyto/genotoxic activities against normal non-target cells as well, there is a lack of information regarding the underlying mechanism involved...
Article
Multielemental analysis of whole blood can provide significant information for the evaluation of nutritional status and diagnosis of certain diseases as well as for the assessment of exposure to potentially toxic metals. However, the quantification of multiple elements in whole blood is not easy partly because of the wide variation in element conce...
Article
Metallothioneins (MTs) exhibit binding affinity for several essential and toxic trace elements. Previous studies in rodents indicated sex differences in the hepatic and renal expression of MTs and concentrations of various elements. The mechanism responsible for these differences has not been resolved. Here, in the liver and kidney tissues of sham-...
Conference Paper
Telomeres are the main guardians of genome stability. During DNA replication, progressive shortening of telomeres takes place with each cell division. Critically short telomeres induce cell cycle arrest or apoptosis. Oxidative stress also plays an important role in telomere dynamics. Recent evidence highlights direct molecular connection between te...
Article
The non-proteinogenic amino acid ß-N-methylamino-l-alanine (BMAA) is associated with the development of neurodegenerative diseases such as Alzheimer's disease, amyotrophic lateral sclerosis/parkinsonism-dementia complex (ALS-PDC) and amyotrophic lateral sclerosis. BMAA is known to induce neurotoxic effects leading to neurodegeneration via multiple...
Conference Paper
Full-text available
Water channels (aquaporins; AQPs) facilitate the passive movement of water across cell membranes. In mammalian kidneys, AQP1 and AQP2 mediate water reabsorption from primary urine across the apical membrane of proximal and distal nephron segments. Studies indicate that renal functional/structural changes in humans and experimental animals develop w...
Conference Paper
Full-text available
Mechanisms of aging are poorly understood. Aging is associated with loss of renal function and structure. Elevated tissue concentrations of reactive oxidative species, known to be present in old humans and experimental animals, may affect the expression and/or activity of various renal transporters, including those that mediate reabsorption of gluc...
Article
The cytokinesis-block micronucleus cytome (CBMN Cyt) assay was used to evaluate the baseline frequency of cytogenetic damage in peripheral blood lymphocytes of the general population (average age, 38.28 ± 12.83 years) in relation to age, sex, body mass index, seasonal variations (season of sampling, period of sampling and different meteorological p...
Article
Cytostatic drugs are highly cytotoxic agents used in cancer treatment and although their benefit is unquestionable, they have been recognized as hazardous to healthcare professionals in occupational settings. In a working environment, simultaneous exposure to cytostatics may occur creating a higher risk than that of a single substance. Hence, the p...
Article
Since there are several predicting factors associated with the comet assay parameters, we have decided to assess the impact of seasonal variations on the comet assay results. A total of 162 volunteers were retrospectively studied, based on the date when blood donations were made. The groups (winter, spring, summer and autumn) were matched in terms...
Article
Thyroid cancer represents 2.1% of new cancer cases, yet it is the most common endocrine cancer and one of the fastest growing cancer types in the world. The incidence of thyroid cancer is the highest in elderly population (60-69 years), but novel data suggest decline in age when this cancer is frequently diagnosed to 50-59 years. Thyroid cancer is...
Article
Telomeres are dynamic chromosome-end structures that serve as guardians of genome stability. They are known to be one of the major determinants of aging and longevity in higher mammals. Studies have demonstrated a direct correlation between telomere length and life expectancy, stress, DNA damage, and onset of aging-related diseases. This review dis...
Article
The selective tyrosine kinase inhibitor imatinib mesylate (IM) is a widely used anticancer drug. Recent studies showing that IM can induce DNA and chromosomal damage in crustaceans and higher plants prompted us to re-examine its potential genotoxicity. IM was not mutagenic in the Ames assay (Salmonella typhimurium). Cytotoxicity and genotoxicity we...
Article
Since the incidence of cancer has increased over the years, adequate prevention programmes are needed. Thyroid cancer is one of the fastest growing cancer types in the world. In this study we performed a case-control study of 100 untreated patients with thyroid diseases (papillary thyroid cancer, follicular thyroid adenoma, and other thyroid diseas...
Article
Since the production of printed circuit boards (PCBs) generates wastewater contaminated with heavy metals and organic matter, PCB factories represent potential pollution sites. The wastewater toxicologically tested in this study contained several metals and the most abundant were copper and iron. At two exposure times tested (4 and 24 h) PCB wastew...
Conference Paper
Aging is a physiological process characterized by a reduced ability to respond to environmental stressors. Despite many aging theories, this process is still poorly understood. As evidenced in recent studies, aging may be associated with increased oxidative stress (OS), which may affect an organ’s functional and morphological characteristics. React...
Article
Circulating platinum (Pt) is detectable in the blood of Pt-treated cancer patients for over a decade after the treatment. Prolonged exposure to Pt, in combination with adverse compounds from nutrition and lifestyle, such as cadmium (Cd), could increase the risk from second cancers. The aim of this study was to investigate the effects of simultaneou...
Article
In the present study toxicity of Frangula alnus Mill. bark, widely used as laxative, was investigated. Human peripheral blood lymphocytes (HPBLs) were treated with F. alnus bark extract or emodin (emodin is bark component with laxative property), and cytotoxicity, genotoxicity and parameters of oxidative stress were assessed. Also, polyphenol conte...
Article
Due to their increasing use, the residues of anti-neoplastic drugs have become emerging pollutants in aquatic environments. Most of them directly or indirectly interfere with the cell's genome, which classifies them into a group of particularly dangerous compounds. The aim of the present study was to conduct a comparative in vitro toxicological cha...
Article
Thyroid cancer is one of the fastest growing cancer types worldwide. Using the cytokinesis-block micronucleus (CBMN) and comet assays, we performed a case-control study of 23 untreated papillary thyroid cancer (PTC) patients and 23 healthy volunteers. PTC patients showed higher basal DNA damage in peripheral blood lymphocytes. The CBMN assay indica...
Article
Full-text available
Introduction: Beneficial influence of vegetarian dietary habits in reducing common risk factors of metabolic syndrome has been recently evidenced. However, adiponectin production and secretion has been scarcely studied in vegetarians, despite its important potential in recovering metabolic homeostasis by reducing inflammation and insulin resistance...
Article
Residues of anti-neoplastic drugs represent new and emerging pollutants in aquatic environments. Many of these drugs are genotoxic, and it has been postulated that they can cause adverse effects in aquatic ecosystems. 5-Fluorouracil (5-FU) is one of the most extensively used anti-neoplastic drugs in cancer therapy, and this article describes the re...
Article
The aim of this study was to investigate the possible protective effect of sodium copper chlorophyllin (CHL) against cytotoxicity and DNA damage induced by mycotoxins ochratoxin A (OTA) and fumonisin B1 (FB1). CHL (0.1-100μg/ml) alone had no impact on cell viability and genome damage in the primary human peripheral blood lymphocytes (HPBLs) and exh...
Article
The purpose of this work was detailed physicochemical, radiological, and toxicological characterization of the composite sample of water intended for human consumption in the Cameron/Tuba City abandoned uranium mining area before and after a combined electrochemical/advanced oxidation treatment. Toxicological characterization was conducted on human...
Article
Full-text available
Abstract Background The main objective of the present study was to investigate chemical composition and possible cyto/genotoxic potential of several medical implant materials commonly used in total hip joint replacement. Methods and Results For that purpose medical implant metal alloy (Ti6Al4V and CoCrMo) and high density polyethylene particles we...
Article
In the present study the alkaline comet assay and the cytokinesis-block micronucleus cytome (CBMN Cyt) assay were used to evaluate the baseline frequency of cytogenetic damage in peripheral blood lymphocytes (PBLs) of 50 healthy children from the general population in Croatia (age, 11.62±1.81 years). Mean values of tail length, tail intensity and t...
Article
Full-text available
The present study aimed to characterize chemical properties of clay bricks containing 20 % of sewage sludge. After detection of potentially hazardous metals, we simulated precipitation exposure of such material to determine the amount of heavy metals that could leach out of the bricks. Metals, such as copper, zinc, nickel, cobalt, chromium, etc., w...
Conference Paper
: Forty years after the use of DDT has been restricted to malaria affected areas, this pesticide and its metabolites can be detected in water, food, and human tissue around the world. People chronically exposed to DDT have higher risk of suffering from hormonal, reproduction, and psychiatric illnesses. Also, DDT has possible carcinogenic effect on...
Conference Paper
Landfill leachate contains high amount of pollutants that can contaminate ecosystems, and in that manner can pose a risk not only to environment but to human health as well. The aim of this research was toxicological characterization of the landfill leachate prior/after the treatment using two different approaches on human lymphocytes as sensitive...
Article
Radiation protection depends on many factors that can be divided on the ground of technical and human factors that are both equally relevant for the radiation protection. To provide the best care for the young patients the effects of radiation should be minimized in accordance with ALARA (As Low As Reasonably Achievable) principle. Chest radiograms...
Chapter
Radiation protection depends on many factors that can be divided on the ground of technical and human factors that are both equally relevant for the radiation protection. To provide the best care for the young patients the effects of radiation should be minimized in accordance with ALARA (As Low As Reasonably Achievable) principle. Chest radiograms...

Network

Cited By

Projects

Projects (3)
Project
HUMNap will determine possible associations between the air pollutants and biomarkers of exposure and early biological effect. The project will start with investigations at multiple locations with different air pollution levels and origin followed by measurement of various environmental airborne pollutants. The next step will be a detailed assessment of different biomarkers of exposure and early biological effects (genomic instability and oxidative stress) in human populations living in those locations. HUMNap will promote state-of-art techniques and research approaches to develop risk assessments of human exposure to airborne pollutants. The results from HUMNap will demonstrate how airborne pollutants affect early molecular events important for disease development in different human cells. It will also provide an assessment of cancer risk among human populations affected by polluted urban areas. Moreover, HUMNap aims to draw the attention of many stakeholders such as leading scientists, policy makers, industry, and the public in order to raise awareness regarding air pollution and to develop monitoring regimes. Finally, HUMNap will provide new datasets necessary for scientifically based risk assessments of human populations exposed to urban air pollution. Financed by the Croatian Science Foundation (HRZZ)
Project
The society’s MISSION is to bring together radiation researchers from around the world to: 1. Discuss new results of studies of ionizing radiation effects in humans, epidemiologic methodology and dosimetry 2. Support capacity building and training for the next generation of epidemiologists, statisticians and dosimetrists 3. Promote and facilitate international collaborations ISORED will organize periodic scientific meetings which will be held in cities around the world that offer affordable meeting options, will have low registration fees and student scholarships to improve access for the next generation of scientists and those from low and middle-income countries.
Project
Aging is a physiological process characterized by a gradual decline in physical fitness and other functional capabilities. The mechanisms of aging and possible actions of slowing down or ameliorating aging-related processes are poorly understood. In humans, one of the characteristics of old age is polypharmacy, which elevates risks of drug-drug interactions and drug-induced adverse reactions and organ toxicity, such as hepatotoxicity and nephrotoxicity. These reactions may partially result from diminished drug metabolism and/or mainly from impaired activity/expression of various membrane transporters that are responsible for drug excretion in liver and kidneys. These both mechanisms have been poorly studied in senescent organs. Here we promote a hypothesis that the aging-induced oxidative stress, known to downregulate the activity/expression of the aging suppressor gene (Klotho) and to shorten the length of telomeres, also diminishes the activity/expression of various membrane transporters in liver and kidneys, and that these changes can be ameliorated by treating animals with antioxidants resveratrol and melatonin. The extent and the intensity of aging-related changes, and the beneficial effects of antioxidants may be sex-related. To test this hypothesis, we propose various biochemical and cell- and molecular-biological studies of aging parameters and aging-dependent expression of various membrane transporters in the mammalian liver and kidneys using a rat model of experimental aging. Possible aging-related decline in the expression of these transporters may explain a higher incidence (women > man) of drug interactions and toxicity in old people. The studies will define the underlying cellular mechanisms that contribute to generation of the aging-related pathophysiological conditions in the liver and kidneys, and will indicate possible ways of their amelioration. Positive results may point to remedies to be used for improving the health of elderly people. Project leaders: Ivan Sabolic, MD, PhD and Davorka Breljak, PhD