Marisa Ana Andrada Borzollino

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• Doctor of Philosophy
• Professor at University of Las Palmas de Gran Canaria

In 2023, an unprecedented outbreak of highly pathogenic avian influenza (HPAI) H5N1 resulted in the death of thousands of pinnipeds along the Argentinean coast, raising concerns about its ecological and epidemiological impact. Here, we present clinical, pathological, and molecular findings associated with HPAI H5N1 infection in pinnipeds from Chubu...

Simple Summary Paratuberculosis (PTB) is a chronic disease that affects domestic and wild ruminants worldwide. This study was conducted in 12 diary caprine farms on the Canary Islands. The region counts with the fourth largest goat population in Spain and has “officially free” bovine tuberculosis status. Two sampling sessions were conducted, and 27...

Samples from the mesenteric lymph nodes (MS LNs) and ileocecal valves (ICV) of 105 goats, comprising 61 non-vaccinated and 44 vaccinated against Mycobacterium avium subspecies paratuberculosis (MAP), were collected at slaughter from a farm with a confirmed history of paratuberculosis (PTB). These goats had subclinical infections. PTB-compatible les...

Fifty-five skin lesions from 31 stranded cetaceans along the Canary coasts (2011–2021) were submitted to macroscopic, histological, and molecular analyses to confirm infection by cetacean poxvirus, herpesvirus and cetacean morbillivirus. They were macroscopically categorized into eight categories with respective subcategories according to their col...

Simple Summary Paratuberculosis (PTB) is a well-known disease with considerable financial impact on the farm industry worldwide. Nevertheless, data regarding the assessment of naturally infected goat herds is limited. The present study describes in detail the observed gross and histological lesions detected in 39 necropsies of goats (15 vaccinated...

In this study we undertook a comprehensive analysis of a Pet Tumour Registry of the Canary Archipelago (PTR-CA) in Spain to investigate the epidemiology of canine cutaneous round cell tumours. From a database of 2526 tumours collected from 2003 to 2020, we conducted a longitudinal analysis of the main trends in diagnosis, age, multiplicity and anat...

Este articulo presenta una revisión de la necesidad y situación general del soporte audiovisual para la formación en el ámbito científico técnico universitario dentro del conjunto de las universidades públicas españolas, contextualizado en la exigencia creciente de virtualización, consecuencia de la aceleración continua de la actividad formativa en...

Simple Summary Neuromelanin is a dark pigment that is present in several types of neurons of the brain. The role of human neuromelanin is a matter of controversy and, over the past few years, has been attributed to having a dual nature, either in a protective role to shield neurons from toxic compounds, or as a trigger of neuroinflammation. This pi...

Paratuberculosis (PTB) is a disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), which affects a broad range of hosts, including domestic and wild animals. PTB is a chronic granulomatous enteritis and lymphadenitis that compromises animal welfare and causes economic losses. The objective of this study was to evaluate the effect...

Nearly two decades ago, pathologic examination results suggested that acoustic factors, such as mid-frequency active naval military sonar (MFAS) could be the cause of acute decompression-like sickness in stranded beaked whales. Acute systemic gas embolism in these whales was reported together with enigmatic cystic liver lesions (CLL), characterized...

In collaboration with the American College of Veterinary Pathologists.

Considerable information has been gained over the last few decades on several disease processes afflicting free-ranging cetaceans from a pathologist's point of view. Nonetheless, there is still a dearth of studies on the hearts of these species. For this reason, we aimed to improve our understanding of cardiac histological lesions occurring in free...

Nearly two decades ago, pathologic examination results suggested acoustic factors, such as mid-frequency active naval military sonar (MFAS) could be the cause of acute decompression-like sickness in stranded beaked whales. Acute systemic gas embolism in these beaked whales was published together with enigmatic cystic liver lesions (CLL), characteri...

Swine respiratory disease is associated with productive losses. We evaluated the prevalence of lung lesions with an emphasis on Mycoplasma hyopneumoniae (Mh), porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), as well as the impact on productive parameters in 108 finishing pigs at slaughter. Pathologic...

Simple Summary Herpesviruses (HVs) are a large family of DNA viruses infecting animals (including insects and mollusks) and humans. Cetaceans can be also infected by HVs presenting different range of lesions, from dermatitis to meningoencephalitis, or being asymptomatic. Several studies have addressed the question of HVs in cetaceans, although no p...

Decompression sickness (DCS) is a widely known clinical syndrome in human medicine, mainly in divers, related to the formation of intravascular and extravascular gas bubbles. Gas embolism and decompression-like sickness have also been described in wild animals, such as cetaceans. It was hypothesized that adaptations to the marine environment protec...

Simple Summary: Hearing is arguably the primary sensory and communication channel for cetaceans. The study of diverse physiological and pathological events involving this system, requires standardized and reliable protocols for processing valuable and scarce samples such as the ears of cetaceans. As part of our research, standardized tissue process...

Aggressive encounters involving cetacean species are widely described in the literature. However, detailed pathological studies regarding lesions produced by these encounters are scarce. From January 2000 to December 2017, 540 cetaceans stranded and were necropsied in the Canary Islands, Spain. Of them, 24 cases of eight species presented social tr...

Capture myopathy (CM), is a syndrome that occurs as the result of the stress during and after capture, handling, restraint, and transport of wild animals. Although CM has been described for many species of cetaceans, characterization of the acute cardiac injury - an important component of this syndrome - are still scarce. In this study, we firstly...

The prostate is the only male accessory gland in cetaceans. However, little is known about this organ in these species. Anatomical and histological characteristics of the prostate have been described in only a few cetacean species, further, one study reported a high incidence of prostatic pathologies in cetaceans that may impair reproduction. The o...

Strikes between vessels and cetaceans have significantly increased worldwide in the last decades. The Canary Islands archipelago is a geographical area with an important overlap of high cetacean diversity and maritime traffic, including high-speed ferries. Sperm whales (Physeter macrocephalus), currently listed as a vulnerable species, are severely...

This study describes the pathologic findings and most probable causes of death (CD) of 224 cetaceans stranded along the coastline of the Canary Islands (Spain) over a 7-year period, 2006–2012. Most probable CD, grouped as pathologic categories (PCs), was identified in 208/224 (92.8%) examined animals. Within natural PCs, those associated with good...

Tissues submitted for microbiological analysis and results from a subset of 224 stranded and necropsied cetaceans. (DOCX)

Main morphologic and etiologic diagnoses in animals included in ‘natural pathology associated with significant loss of nutritional status’. (DOCX)

Main morphologic and etiologic diagnoses in animals included in ‘vessel collision’. (DOCX)

Main morphologic and etiologic diagnoses in animals included in ‘pathology associated with good nutritional status’. (DOCX)

Main morphologic and etiologic diagnoses in animals included in ‘neonatal and perinatal pathology’. (DOCX)

Main morphologic and etiologic diagnoses in animals included in ‘intra- and interspecific traumatic interactions’. (DOCX)

Biological and epidemiological data of 224 stranded and necropsied cetaceans. Sex: female (F), male (M). Age: fetus (F), neonate (N), calf (C), juvenile (Jv), subadult (Sad), adult (Ad). Stranding date (SD; mm/dd/yy). Type of stranding (TS). Stranding location, island (IS): Gran Canaria (GC), Fuerteventura (FT), Lanzarote (LZ), Tenerife (TF), La Go...

Details of immunohistochemical analyses performed on formalin-fixed, paraffin-embedded tissue sections from selected cetacean species, including primary antibody (Ab), manufacturer, clonality, dilution, pretreatment, incubation, secondary Ab, manufacturer, and visualization system. CD: Cluster of differentiation; CK: cytokeratin; GFAP: glial fibril...

Main morphologic and etiologic diagnoses in animals included in ‘interaction with fishing activities’. (DOCX)

Main morphologic and etiologic diagnoses in animals included in ‘foreign body-associated pathology’. (DOCX)

The vascular system of Cuvier’s beaked whales (CBW) (Ziphius cavirostris; family Ziphiidae), an extremely deep, prolonged-diving cetacean, is increasingly receiving anatomic and physiologic study due to possible anthropogenic interactions; however, vascular pathology rarely has been reported in this species. Thirteen CBW stranded in the Canary Isla...

http://dx.doi.org/10.3201/eid2204.150954

Technical Appendix. Microscopic images of tissue samples from 2 short-finned pilot whales (Globicephala macrorhynchus) from the eastern Atlantic Ocean stranded along the Canary Islands, Spain, 2015.

We describe gross, histopathologic, ultrastructural, immunohistochemical, and microbiologic features of acute septicemia by Erysipelothrix rhusiopathiae in an Atlantic spotted dolphin (Stenella frontalis) and an Atlantic bottlenose dolphin (Tursiops truncatus). Generalized lymphadenomegaly and widespread hemorrhages were the most consistent macrosc...

Involvement of Merkel cells (MKs) in different cutaneous diseases as well as in the growth, differentiation and homeostasis of the skin has been previously documented. The aim was to assess the ultrastructural features of MKs in canine skin, including morphometrics, highlighting their similarities with and differences from those described for other...

An adult male striped dolphin (Stenella coeruleoalba) stranded alive at Arico, Tenerife, Canary Islands, Spain. The dolphin died shortly after stranding, and a complete postmortem examination was performed. The most remarkable gross findings were two fleshy masses of approximately 1 cm diameter, near the tip of the penis. These masses were composed...

A uterine prolapse associated with a leiomyoma (fibroid) was observed in a live-stranded Atlantic spotted dolphin (Stenella frontalis). A 7 cm segment of the reproductive tract including the cervix, uterine neck and caudal uterine body had intussuscepted and prolapsed into the cranial vaginal vault. In the leading edge of the intussuscepted/prolaps...

This is the first work that applies immunohistochemistry in the characterisation of the inflammatory infiltrate of verminous bronchopneumonia associated with lungworm parasites in stranded dolphins. Samples from three different species (Stenella coerulealba, Stenella frontalis and Delphinus delphis) stranded in the Canary Islands were used. The mos...

14-3-3σ is a cell cycle regulator that has been designed as a ‘double-edged sword’ in the context of human cancers, in which in some it appears to function as a tumour suppressor with decreased expression contributing to tumourigenesis, while in others it may be involved in tissue invasion and metastasis. A variety of studies in both human and cani...

Abstract Wohlfahrtiimonas chitiniclastica is a rare cause of fatal septicemia in humans, and recently reported in a white-tailed deer (Odocoileus virginianus). We describe fatal septicemia associated with W. chitiniclastica in a short-beaked common dolphin (Delphinus delphis) with fibrinosuppurative and necrotizing pulmonic, aortic, and mitral valv...

Merkel cells (MCs) are specialized skin receptors characterized by their particular location and close association with nerve terminals. They also are cells with a presumptive neuroendocrine function and are considered as part of the diffuse neuroendocrine system. By using commercially available monoclonal and polyclonal antibodies in samples of do...

Porcine enzootic pneumonia, primarily caused by Mycoplasma hyopneumoniae (Mh), is a contagious disease characterized by catarrhal bronchointerstitial pneumonia. Previous studies have evaluated immunohistochemically the distribution of Mh, different cellular populations and cytokines during Mh-induced pneumonia. Cyclooxygenase (COX)-2 is overexpress...

This report describes the pathological findings in an adult female short-beaked common dolphin (Delphinus delphis) stranded alive in the Canary Islands. Necropsy examination revealed the presence of a nodular neoplastic growth in the central nervous system (CNS) at the level of the thalamus. Microscopical examination revealed the mass to be a lymph...

Ship strikes are a major issue for the conservation of may cetacean species. Certain gross and microscopic criteria have been previously reported for establishing a diagnosis of death due to ship strikes in these animals. However, some ship-strike injuries may be masked by advanced carcass decomposition and may be undetectable due to restricted acc...

A systemic morbillivirus infection was diagnosed postmortem in a juvenile bottlenose dolphin stranded in the eastern North Atlantic Ocean in 2005. Sequence analysis of a conserved fragment of the morbillivirus phosphoprotein gene indicated that the virus is closely related to dolphin morbillivirus recently reported in striped dolphins in the Medite...

Caprine coccidiosis, affecting mainly young goat kids around the weaning period, is worldwide the most important disease in the goat industry. Control of caprine coccidiosis is increasingly hampered by resistances developed against coccidiostatic drugs leading to an enhanced need for anticoccidial vaccines. In the current study we conducted an oral...

##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

Wild birds share with humans the capacity for moving fast over large distances. During migratory movements, birds carry pathogens that can be transmitted between species. One of these concerning pathogens is Mycobacterium spp. A necropsy was performed in a grey heron (Ardea cinerea) that had been medically treated for a polyarthritic process. Gross...

This paper describes the immunophenotype of cellular inflammatory infiltrates in chronic cholangitis in six common dolphins (Delphinus delphis), four striped dolphins (Stenella coeruleoalba), three Atlantic spotted dolphins (Stenella frontalis) and one pygmy sperm whale (Kogia Breviceps) found stranded along the coasts of the Canary Islands (Spain)...

Coccidiosis caused by the apicomplexan protozoa Eimeria ninakohlyakimovae has a high impact on animal health and profitability of the goat industry worldwide. Primary E. ninakohlyakimovae infections induce clinical disease mainly in goat kids, as homologous re-infections are commonly under immunological control. Nevertheless, there is evidence of f...

We describe two cases of adrenohepatic fusion (AHF) in domestic ferrets (Mustela putorius furo). This condition is defined as the union of hepatic tissue with the adrenal gland with close fusion of the respective parenchymal cells and lack of a fibrous capsule between the two cell populations. AHF is believed to be a congenital anomaly caused by fa...

Between 1999 and 2005, 233 stranded cetaceans (comprising 19 species) were reported in the waters of the Canary Islands. Of these, 138/233 (59.2%) were subjected to a complete or partial standardized necropsy, including 4 Balaenopteridae, 9 Physeteridae, 8 Kogiidae, 27 Ziphiidae and 90 Delphinidae. Of these, 46/138 (33.3%) cetaceans were diagnosed...

The aim of this study was to investigate the histochemical parameters of muscle fibre in a Spanish autochthonous pig breed. Due to its fatty meat and high prolificacy, this pork is revered among local consumers. However, the aforementioned breed has become endangered. A total of 11 native pigs (Black Canary Pig) were evaluated. longissimus lumborum...

The study of the expression of 14-3-3σ, p53, and vimentin proteins in canine transitional cell carcinoma (TCC) evaluating differences with normal bladder tissues, and the association with clinicopathological variables. We analyze by immunohistochemistry in 19 canine TCCs the expression of 14-3-3σ, p53, and vimentin using monoclonal antibodys. A sem...

Background The capacity for herpesvirus to cause disease in cetaceans is unclear and may be varied depending on the different conditions of individuals and between different species. Kidney pathology and intralesional virus-associated infection have been rarely reported in cetaceans. Result On April 2004, an old adult male Blainville’s beaked whal...

A diagnosis of mixed testicular neoplasia in a short beaked common dolphin Delphinus delphis involving a Sertoli cell tumor, an interstitial (Leydig) cell tumor and a seminoma is presented. Lymphatic spread of the Sertoli cell tumor to an adjacent retroperitoneal lymph node was observed. Testicular neoplasms have been infrequently reported in marin...

Gas-bubble lesions were described in cetaceans stranded in spatio-temporal concordance with naval exercises using high-powered sonars. A behaviourally induced decompression sickness-like disease was proposed as a plausible causal mechanism, although these findings remain scientifically controversial. Investigations into the constituents of the gas...

The current study was conducted to isolate a field strain of Eimeria ninakohlyakimovae, characterize its infectivity and the response to challenge under experimental conditions. The isolated strain (GC) induced a prepatent period of 14-15days p.i., a patency of 7±2days and a noticeable pathogenicity in infected goat kids. Challenge trials resulting...

Mycoplasma hyopneumoniae (Mh) is the primary agent of porcine enzootic pneumonia. The predominant histological lesion is hyperplasia of the bronchus-associated lymphoid tissue (BALT). Livingston et al. (1972) classified these lesions into four stages. The aim of this study was to determinate the severity of the histological lesions, quantify and lo...

Vascular tumors and disorders, like angiomatosis, are rarely described in cetacean species. A retrospective histological study was carried out on lung samples from 35 common dolphins (Delphinus delphis) stranded in the Canary Islands coasts looking for morphological vascular changes and likely related causes. Twenty-five out of thirty-five (71%) co...

Skeletal muscle samples were examined post-mortem in 148 cetaceans over a 12-year period. Histolog-ical analysis included haematoxylin and eosin (HE) and periodic acid-Schiff (PAS) staining with and without diastase digestion. In addition, histological muscle sections were immunostained for ubiquitin and fast and slow heavy-chain myosin isoforms. P...

In July 2004, four Ziphius cavirostris stranded in the Canary Islands several days after an international naval exercises were conducted north of the Canary Islands. During the maneuvers high intensity mid-frequency sonar was used. Three of the animals were fully necropsied. Abundant fresh non-digestive aliment was found in all stomachs. Hemorrhage...

Citrobacter freundii, a gram-negative enterobacterium, may cause fatal septicemia in humans and animals. Its potential pathogenic role in cetaceans (bottlenose dolphins and beluga whales) has been hypothesized. Here we describe fatal C. freundii septicemia in a stranded newborn Cuvier's beaked whale (Ziphius cavirostris).

To explore the pathogenesis of caprine contagious agalactia (CA), we assessed the ability of Mycoplasma agalactiae (Ma) to modulate the immune system in host tissues by immunohistochemically and chronologically characterizing the main cell subsets present during the mammary immunoinflammatory response. Fifteen lactating goats were inoculated with 1...

Lymph nodes from 186 cows were evaluated as part of a bovine tuberculosis eradication programme. The mediastinal lymph nodes of 13 animals contained atypical structures. In 12 cases (6.45%) these consisted of multiple epithelial structures and, in one case, of pancreatic-like tissue. Immunohistochemistry (IHC) revealed that the epithelial structure...

A 4-year-old female Appaloosa horse was referred to the Department of Comparative Pathology, Veterinary Faculty, with a history of colic and acute respiratory distress. At necropsy, gross lesions consisted of extensive firm, multinodular, hemorrhagic foci in fat tissues with yellow-brown discoloration. The most affected areas were peritoneal fat an...

To correlate the clinical course of mycoplasma mastitis with its immune response, right mammary glands of 15 lactating goats were inoculating with 10(10) colony-forming units (cfu) of Mycoplasma agalactiae (Ma). Before sacrificing the animals at 5, 15 or 45 days post-inoculation (dpi), blood Ma antibody titres and milk mycoplasma colony and somatic...

The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without...