Mario Henry Rodríguez

Mario Henry Rodríguez
Instituto Nacional de Salud Pública | INSP · Center for Research on Infectious Diseases

pHd

About

364
Publications
30,853
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
8,382
Citations
Citations since 2016
57 Research Items
3335 Citations
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
Introduction
Mario Henry Rodríguez currently works at the Center for Research on Infectious Diseases, Instituto Nacional de Salud Pública. Mario does research in Molecular Biology, Microbiology and Parasitology. Their current projects are 'Developing an evidence-based policy framework for dengue prevention and control, Haemopiesis in Aedes aegypti, triatoma-gut microbiota interactions, and molecules participating in immunity in Triatominae.
Skills and Expertise
Additional affiliations
January 2012 - January 2016
Instituto Nacional de Salud Pública
Position
  • Senior Researcher

Publications

Publications (364)
Article
Full-text available
The circulation of the four-dengue virus (DENV) serotypes has significantly increased in recent years, accompanied by an increase in viral genetic diversity. In order to conduct disease surveillance and understand DENV evolution and its effects on virus transmission and disease, efficient and accurate methods for phylogenetic classification are req...
Article
Full-text available
Background Antimicrobial peptides (AMPs) participate in the humoral immune response of insects eliminating invasive microorganisms. The immune deficiency pathway (IMD) and Toll are the main pathways by which the synthesis of these molecules is regulated in response to Gram-negative (IMD pathway) or Gram-positive (Toll pathway) bacteria. Various pat...
Article
Full-text available
Dengue fever is caused by four related dengue virus serotypes, DENV-1 to DENV-4, where each serotype comprises distinct genotypes and lineages. The last major outbreak in Mexico occurred during 2012 and 2013, when 112,698 confirmed cases were reported (DENV-1 and DENV-2 were predominant). Following partial E, NS2A and NS5 gene sequencing, based on...
Article
Full-text available
Chikungunya virus (CHIKV) is an alphavirus transmitted by Aedes mosquitoes, which causes Chikungunya fever. Three CHIKV genotypes have been identified: West African, East-Central-South African and Asian. In 2014, CHIKV was detected for the first time in Mexico, accumulating 13,569 confirmed cases in the following three years. Studies on the molecul...
Article
Full-text available
Background Flying is an essential function for mosquitoes, required for mating and, in the case of females, to get a blood meal and consequently function as a vector. Flight depends on the action of the indirect flight muscles (IFMs), which power the wings beat. No description of the development of IFMs in mosquitoes, including Aedes aegypti, is av...
Article
Full-text available
The transmission of Plasmodium parasites in residual foci is currently a major roadblock for malaria elimination. Human activities and behavior, along with outdoor biting mosquitoes with opportunistic feeding preferences are the main causes of the inefficacy of the main vector control interventions, long lasting insecticide-impregnated nets and ins...
Article
Full-text available
Insect neuropeptides, play a central role in the control of many physiological processes. Based on an analysis of Nyssorhynchus albimanus brain transcriptome a neuropeptide precursor database of the mosquito was described. Also, we observed that adipokinetic hormone/corazonin-related peptide (ACP), hugin and corazonin encoding genes were differenti...
Article
The heat shock protein family 70 (Hsp70) comprises chaperone proteins that play major multiple roles in Plasmodium asexual and sexual development. In this study, we analyzed the expression of Hsp70-1 in gametocytes, gametes, zygotes, and its participation in ookinete formation and their transition into oocysts. A monoclonal antibody against recombi...
Book
Full-text available
Se presenta una guía para la producción de un pez nativo de la Cuenca del Río Balsas Poecilia maylandi, para el control del larvas de mosco del género Aedes spp, que son vectores del Dengue y otras enfermedades.
Article
Full-text available
Objective: The feasibility of the use of WHO impregnated paper and biochemical assays to determine lethal concentrations (LC50 and LC99) and insecticide metabolic enzyme levels of Triatoma dimidiata. Materials and methods: LC50 and LC99 were calculated with WHO papers impregnated at different concentrations of malathion, propoxur and deltamethri...
Article
Full-text available
In the southern Pacific coast of Chiapas, Mexico (SM), the two most abundant vector species, Nyssorhynchus albimanus and Anopheles pseudopunctipennis, were susceptible to different Plasmodium vivax Pvs25/28 haplotypes. To broaden our understanding of the existing P. vivax in the area, genes encoding proteins relevant for ookinete development and th...
Article
Full-text available
Objective: To research mutations associated to pyrimethamine resistance in dihydrofolate reductase (pvdhfr) of Plasmodium vivax from Mexico and Nicaragua and compare it to that reported in the rest of America. Materials and methods: Genomic DNA was obtained from P. vivax-infected blood samples. A pvdhfr gene fragment was amplified and sequenced....
Article
Background: Aedes aegypti and Aedes albopictus are the main mosquito species responsible for dengue virus (DENV) transmission to humans in the tropical and subtropical regions of the world. The role of vertical transmission in the epidemiology of dengue and the maintenance of this arbovirus in nature during interepidemic periods remain poorly unde...
Article
Full-text available
Background: The susceptibility of Anopheles albimanus and An. pseudopunctipennis to local Plasmodium vivax has been associated in southern Mexico with two ookinete surface proteins (Pvs25/28) polymorphism. Perhaps parasite population selection (i.e. adaptation to local vectors) contributes to this phenomenon. It is also possible that certain molec...
Article
Full-text available
Background Chagas disease is a parasitic infection caused by Trypanosoma cruzi. It is an important public health problem affecting around seven to eight million people in the Americas. A large number of hematophagous triatomine insect species, occupying diverse natural and human-modified ecological niches transmit this disease. Triatomines are long...
Article
Full-text available
Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage. Many studies suggest that the parasite binds to midgut-specific glycans. We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccu...
Article
Full-text available
Background: Insects operate complex humoral and cellular immune strategies to fend against invading microorganisms. The majority of these have been characterized in Drosophila and other dipterans. Information on hemipterans, including Triatominae vectors of Chagas disease remains incomplete and fractionated. Results: We identified putative immun...
Article
Full-text available
Objective: To design and analyze the efficacy of an Ecohealth competency-based course on the prevention and control of vector-borne-diseases for specific stakeholders. Materials and methods: Multiple stakeholders and sectors of the region were consulted to identify Ecohealth group-specific competencies using an adjusted analysis matrix. Eight co...
Article
Full-text available
Objective: To analyze the current knowledge of pathogen-insect interactions amenable for the design of molecular-based control strategies of vector-borne diseases. Materials and methods: We examined malaria, dengue, and Chagas disease pathogens and insect molecules that participate in interactions during their vectors infection. Results: Patho...
Article
Full-text available
Objective: To analyze the association of dengue fever incidence with Aedes mosquito's abundance, and the effect of climatological and geographical variables, in a region in Morelos State, Mexico. Materials and methods: Weekly data during the period 2010 to 2014 was used. Mosquito abundance was determined using ovitraps. Confirmed dengue cases we...
Article
Full-text available
Objective: To analyze the transcription pattern of neuropeptides in the ontogeny of a malaria vector, the mosquito Anopheles albimanus. Materials and methods: The transcription pattern of Crustacean CardioActive peptide (CCAP), corazonin, Ecdysis Triggering Hormone (ETH), allatostatin-A, orcokinin, Insulin Like Peptide 2 (ILP2), Insulin Like Pep...
Article
Full-text available
Background Better knowledge of the innate immune system of insects will improve our understanding of mosquitoes as potential vectors of diverse pathogens. The ubiquitously expressed 14-3-3 protein family is evolutionarily conserved from yeast to mammals, and at least two isoforms of 14-3-3, the ε and ζ, have been identified in insects. These protei...
Article
Full-text available
Ubiquitination tags proteins for different functions within the cell. One of the most abundant and studied ubiquitin modification is the Lys48 polyubiquitin chain that modifies proteins for their destruction by proteasome. In Plasmodium is proposed that post-translational regulation is fundamental for parasite development during its complex life-cy...
Data
Comparison of polyubiquitin genes of different Plasmodium species and ubiquitin secondary structure. (A) Schematic representation of the polyubiquitin gene in P. falciparum (PF3D7_1211800), P. vivax (PVX_084620), P. chabaudi (PCHAS_061200) and P. berghei (PBANKA_061030). The first exon of each species has a length of 28 bp. The intron has a length...
Data
Immunoblot analysis of PcUb recombinant protein against anti-Ubiquitin monoclonal antibody. Specificity of the anti-Ubiquitin monoclonal antibody was tested with the Ubiquitin monomer recombinant protein of P. chabaudi. Lane 1–5. Successive fractions after chromatographic elution of GST-PcUb. (TIF)
Data
Plasmodium chabaudi polyubiquitin sequence. (A) Sequence of the ORF of the polyubiquitin gene (B) Alignment of the DNA sequence of the four tandem repeats of the polyubiquitin gene using Clustal Omega (C) Alignment of the aminoacids sequence of the four tandem repeats of the Ubiquitin polypeptides using Clustal Omega. (DOCX)
Data
Identification of the ubiquitin modified residue by MS/MS. After trypsin digestion, the peptide that contains the post-translational modification has a diglycline remnant covalently attached to a lysine residue that is resistant to the trypsin proteolysis (36). On each figure (A-D) is shown the peptide sequence produced by the trypsin proteolysis w...
Data
Ubiquitinated proteins found in other studies performed in P. falciparum. (DOCX)
Data
Parasitemia of P. chabaudi in male BALB/c mice. A) Male BALB/c mice of 6–8 weeks were infected with 1 x 106 parasites. Every 3 hours the parasite stages were counted. Counts were performed by triplicates, and are expressed as percentage of infected erythrocytes. B) Different intraerythrocytic stages of P. chabaudi obtained after Percoll purificatio...
Data
Proteins identified by MS/MS in ring-, trophozoite-, and schizont-stage parasites. (DOCX)
Data
2-DE gel electrophoretic analysis of P. chabaudi rings, trophozoites, and schizont proteins. Proteins of rings (A), trophozoites (B), and schizonts (C) were resolved on Immobiline DryStrips (pH 3–10, NL 7 cm) and second dimension was performed using 10% SDS-PAGE. Gels were stained with Coomassie Blue G250. Molecular mass markers (kDa) are indicated...
Article
Full-text available
Background Triatomine insects are vectors of Trypanosoma cruzi, a protozoan parasite that is the causative agent of Chagas’ disease. This is a neglected disease affecting approximately 8 million people in Latin America. The existence of diverse pyrethroid resistant populations of at least two species demonstrates the potential of triatomines to dev...
Data
Reference and target transcripts used in qRT-PCR experiments, their primer sequences and amplicon length. (DOCX)
Data
Phylogenies of the mitochondrial CYP clade (Fig 1a), CYP2 clade (Fig 1b), CYP3 clade (Fig 1c), CYP4 clade (Fig 1d), CCE superfamily (Fig 2) and Glutathione Transferase superfamily (Fig 3) from R. prolixus, Triatoma spp., D. melanogaster, An. gambiae, A. mellifera, A. pisum, T. castaneum and B. mori. (DOCX)
Data
Expression of GST, CYP and CCE superfamilies in R. prolixus tissue-specific transcriptomes. The expression of each transcript in the different transcriptomes (anterior and posterior midgut, hindgut, Malpighian tubules, fat body, gonads and whole body) was represented as number of mapped reads. These R. prolixus tissue-specific transcriptomes were c...
Data
Structural analysis of CYP4 (tab1), CYP3 (tab2), GST (tab3) and clade E of CCE (tab4) sequences of R. prolixus and Triatoma spp.; and relation between the contig codes and the assigned names of Triatoma spp. transcripts (tab5) used in Figs 1, 3 and 5 (main text). The structural analysis includes the prediction of number and position for the transme...
Data
Triatoma spp. protein sequences used to construct the phylogenetic trees. (TXT)
Article
The 14-3-3 proteins are evolutionarily conserved acidic proteins that form a family with several isoforms in many cell types of plants and animals. In invertebrates, including dipteran and lepidopteran insects, only two isoforms have been reported. 14-3-3 proteins are scaffold molecules that form homo- or heterodimeric complexes, acting as molecula...
Article
Full-text available
For malaria transmission, Plasmodium parasites must develop in the mosquito vector. Oxidative stress in the insect midgut, triggered by environmental changes (e.g., pH and temperature), influences the cellular signaling of parasites involved in differentiation from gametocytes to mobil ookinetes. It activates the homeostatic response to stress char...
Article
Full-text available
Background Malaria parasites are transmitted by Anopheles mosquitoes. Although several studies have identified mosquito midgut surface proteins that are putatively important for Plasmodium ookinete invasion, only a few have characterized these protein targets and demonstrated transmission-blocking activity. Molecular information about these protein...
Article
Full-text available
The study of human B cell response to dengue virus (DENV) infection is critical to understand serotype-specific protection and the cross-reactive sub-neutralizing response. Whereas the first is beneficial and thus represents the ultimate goal of vaccination, the latter has been implicated in the development of severe disease, which occurs in a smal...
Article
Full-text available
Background: Despite the potential to produce antibodies that can neutralize different virus (heterotypic neutralization), there is no knowledge of why vaccination against influenza induces protection predominantly against the utilized viral strains (homotypic response). Identification of structural patterns of the B cell repertoire associated to h...
Article
Full-text available
In Mexico, combined chloroquine (CQ) and primaquine (PQ) treatment has been used since the late 1950s to treat Plasmodium vivax infections. Although malaria transmission has declined, current treatment strategies must be evaluated to advance towards malaria elimination. The clinical and parasitological outcome of treating symptomatic P. vivax with...
Article
Most of the in-depth studies on insect developmental genetic have been carried out in the fruit fly Drosophila melanogaster, an holometabolous insect, so much more still remains to be studied in hemimetabolous insects. Having R. prolixus sequenced genome available, we search for orthologue genes of zygotic signaling pathways, segmentation, and trac...