Mario Marini

Università degli Studi di Milano-Bicocca | UNIMIB · Department of Physics

Non-linear excitation microscopy offers superior in-vivo imaging but faces challenges in deep tissue. High numerical aperture beams suffer spherical aberrations, while tissue scattering impacts image quality. To address this, we propose implantable microlenses for precise focusing below the skin in lab animals. By using low numerical aperture laser...

Specimen-induced aberrations limit the penetration depth of standard optical imaging techniques in vivo, mainly due to the propagation of high NA beams in a non-homogenous medium. Overcoming these limitations requires complex optical imaging systems and techniques. Implantable high NA micro-optics can be a solution to tissue induced spherical aberr...

Specimen-induced aberrations limit the penetration depth of standard optical imaging techniques in vivo, mainly due to the propagation of high NA beams in a non-homogenous medium. Overcoming these limitations requires complex optical imaging systems and techniques. Implantable high NA micro-optics can be a solution to tissue induced spherical aberr...

Specimen-induced aberrations limit the penetration depth of standard optical imaging techniques in vivo, mainly due to the propagation of high NA beams in a non-homogenous medium. Overcoming these limitations requires complex optical imaging systems and techniques. Implantable high NA micro-optics can be a solution to tissue induced spherical aberr...

In recent decades, the development of ultrashort pulse laser sources has led non-linear optical (NLO) light-matter interaction to acquire a central role in 3D in-vivo, and largely label-free, tissue imaging due to efficient signal generation in the non-linear process [1,2]. Non-linear methods comprise high-harmonic generation (SHG/THG), two-photon...

Deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. In this framework we propose a miniaturized in-vivo imaging window composed of high dioptric power microlenses coupled to micro-scaffold, which were fabricated by two-photon polymerization (2PP) in the biocompati...

In nonlinear optical microscopy through skin, the laser energy is attenuated, and the spot size increases. Holographic corrections work only on small isoplanatic patches. We test Physically-Inspired-Neural-Networks for scattering correction, enhancing speed and overcoming non-isoplanatism.

Ex-vivo histology is time consuming, subjective, and invasive. We discuss how, by coupling non-linear excitation and structured illumination to physically inspired image reconstruction and micro-structuring optical windows, we can obtain in-vivo tissue histology.

Super‐resolution strategies expand the applicability of imaging modalities toward previously inaccessible spatial scales. Herein, photoactivated far‐infrared thermography is pushed from the millimeter to the micrometer scale by a 2D super‐resolution imaging approach capable of tackling the resolution barriers imposed by both diffraction‐limited sig...

A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, deep tissue imaging remains challenging, especially...

Non‐linear excitation microscopy offers several advantages for in‐vivo imaging compared to conventional confocal techniques. However, tissue penetration can still be an issue due to scattering and spherical aberrations induced on focused beams by the tissue. The use of low numerical aperture objectives to pass through the outer layers of the skin,...

Non-linear excitation microscopy offers a number of advantages for in vivo imaging compared to conventional confocal techniques. However, tissue penetration can still be an issue due to both scattering and spherical aberrations induced on highly focused beams by the tissue. The use of low numerical aperture objectives to pass through the outer laye...

The flap of bendable structures under continuous flow impacts a variety of fields, ranging from energy harvesting to active mixing in microfluidic devices. Similar physical principles determine the flapping dynamics in a variety of systems with different sizes, but a thorough investigation of the bending dynamics at the microscale is still lacking....

Super-resolution image acquisition has turned photo-activated far-infrared thermal imaging into a promising tool for the characterization of biological tissues. By the sub-diffraction localization of sparse temperature increments primed by the sample absorption of modulated focused laser light, the distribution of (endogenous or exogenous) photo-th...

Surgical excision followed by histopathological examination is the gold standard for melanoma screening. However, the color-based inspection of hematoxylin-and-eosin-stained biopsies does not provide a space-resolved quantification of the melanin content in melanocytic lesions. We propose a non-destructive photo-thermal imaging method capable of ch...

The possibility to shape stimulus-responsive optical polymers, especially hydrogels, by means of laser 3D printing and ablation is fostering a new concept of “smart” micro-devices that can be used for imaging, thermal stimulation, energy transducing and sensing. The composition of these polymeric blends is an essential parameter to tune their prope...

Two-photon and single photon laser writing are used here to fabricate 3D proteinaceous microstructures with photothermal functionality in the near-infrared spectral region and tunable elasticity. The photo-cross-linking is initiated in bovine serum albumin (BSA) by Rose Bengal and the photo-thermal effect arises from gold nanostars dispersed in the...

Thermal conductivity measurements play a crucial role in most areas of the applied physical sciences, thereby constantly demanding for new non-invasive methods capable of providing high-resolution spatial mapping of absolute thermal conductivities on heterogeneous samples ranging from solid-state bulk materials to low-dimensionality structures. In...

Two‐photon laser writing is used here to fabricate 3D proteinaceous microstructures with photothermal functionality in the near‐infrared spectral region and tunable elasticity. The photo‐cross‐linking is initiated in bovine serum albumin (BSA) by rose bengal or methylene blue and the photo‐thermal effect arises from gold non‐spherically symmetric n...

Active thermal imaging is a valuable tool for the nondestructive characterization of the morphological properties and the functional state of biological tissues and synthetic materials. However, state-of-the-art techniques do not typically combine the required high spatial resolution over extended fields of view with the quantification of temperatu...