Mario Corte RodriguezUniversity of Oviedo | UNIOVI · Department of Physical and Analytical Chemistry
Mario Corte Rodriguez
PhD
About
33
Publications
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631
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Introduction
Additional affiliations
April 2020 - present
University of Oviedo
Position
- PostDoc Position
September 2019 - March 2020
January 2019 - August 2019
Publications
Publications (33)
MicroRNAs (miRNAs), which are short single-stranded RNA sequences between 18 and 24 nucleotides, are known to play a crucial role in gene expression. Changes in their expression are not only involved in many diseases but also as a response to physiological changes, such as physical exercise. In this work, a new analytical strategy for the sensitive...
Streptomyces are important soil bacteria used for bioremediation of metal-contaminated soils, however, it is still unknown how metal-selective Streptomyces are and which mechanisms are involved during their capture. In this work, we exposed S. coelicolor spores to environmentally relevant concentrations (0.1, 1, 10, 100 μM) of Ce, U and Cd in solid...
Background:
This work studies the presence of the Ti, Al and V metal ions and Ti nanoparticles released from the debris produced by the implantoplasty, a surgical procedure used in the clinic, in rat organs.
Methods:
The sample preparation for total Ti determination was carefully optimized using microsampling inserts to minimize the dilution dur...
More than a decade ago, studies on cellular cisplatin accumulation via active membrane transport established the role of the high affinity copper uptake protein 1 (CTR1) as a main uptake route besides passive diffusion. In this work, CTR1 expression, cisplatin accumulation and intracellular copper concentration was assessed for single cells revisit...
In the search for a normalized procedure to replicate and compare single cell-inductively coupled plasma-mass spectrometry (SC-ICP-MS) experiments, SELM-1, a certified reference material containing selenium enriched yeast cells has been used. Selenium concentrations (both, intra- and extracellular) have been measured using either sequential or simu...
Background:
The objective of this study was to evaluate the accumulation of ions in blood and organs caused by Ti metal particles in a mandibular defect in rats, together with a description of the local reaction of oral tissues to these titanium alloy debris.
Methods:
Twenty Sprague-Dawley rats were randomly distributed into three groups: an exp...
Biomedical applications of ICP-MS have increased significantly over the years, not only related to total elemental analysis but to speciation studies. Lately, the development of commercial approaches to permit the analysis of the elemental species in individual cells, the so called single cell ICP-MS (SC-ICP-MS) have added a new dimension to this t...
Copper modulates secondary metabolism in Streptomyces. Although the cytosolic copper concentration is controlled by several chaperones and transporters, the formation of copper nanoparticles and its relation to the antibiotic production has never been established in the model Streptomyces coelicolor. In this work, state-of-the-art analytical tools...
The human epidermal growth factor receptor 2 (HER2) is a transmembrane protein that has become one of the most specific prognostic and predictive biomarker of breast cancer. Its early detection is key for optimizing the patient clinical outcome. This work is focused on the detection of HER2 in individual cells using an antibody containing lutetium...
The bio-tribocorrosion of metallic materials used for dental implants (Ti and alloys) in the oral environment involves the production of metallic debris not only in the ionic, but also in the nanoparticulate form. The present work shows a systematic evaluation of the detection of Ti-containing nanoparticles in different biological media, including...
Ultrasmall iron oxide nanoparticles (<10 nm) were explored here as nanotransporters of cis-diamminetetrachloroplatinum (IV) (a cisplatin prodrug) in cellular models. The coating of the particles containing reactive carboxylic acid groups enabled the formation of a stable conjugate between the prodrug and the nanoparticles using one pot reaction. Th...
Due to the improvements in detection sensitivity, inductively coupled plasma-mass spectrometry (ICP-MS) has matured to be an exceptional tool for the elemental analysis of small objects. The characterisation of individual metallic nanoparticles by so-called single particle ICP-MS (SP-ICP-MS) has paved the way for the analysis of smallest biological...
Assessing the impact of nanoparticles in living systems implies a proper evaluation of their behaviour at single-cell level. Due to the small size of nanoparticles, their accumulation, transformation and location within single cells is challenging. In this work, the combination of single cell/single particle triple quadrupole inductively coupled pl...
One of the limitations in the use of cisplatin is its low penetration into cells. In addition, some cells develop the so called resistance, a multifactorial event that decreases significantly the intracellular cisplatin concentration. To circumvent these limitations, recent studies are focused on the use of nanocarriers that permit, among others, t...
Cell-to-cell variability is known to be of crucial importance to understand different biological processes. Studying the individual variations of, for example, trace elements in cell populations can be carried out only by means of single cell analysis techniques, and, for this aim, single cell elemental analysis by ICP-MS is emerging as a selective...
Several organisms have demonstrated the ability of synthesising biogenic selenium-containing nanoparticles. Such particles from biological sources have attracted great attention due to several proven activities as antioxidants or antimicrobial agents. However, little is known in terms of size (distribution), shapes, chemical composition and number/...
Cells are able to precisely control the amount of iron they acquire in the form of transferrin (TF)-bound iron by modulating the synthesis of the transferrin receptor 1 (TfR1). In tumor cells, elevated TfR1 seems to be related to poorer outcome for patients. Thus, the direct measurement of this biomarker in breast cancer tissues and cells might ser...
Streptomycetes are important biotechnological bacteria with complex differentiation. Copper is a well-known positive regulator of differentiation and antibiotic production. However, the specific mechanisms buffering cytosolic copper and the biochemical pathways modulated by copper remain poorly understood. Here, we developed a new methodology to qu...
One of the main limitations to the Pt-therapy in cancer is the development of associated drug resistance that can be associated to a significant reduction of the intracellular platinum concentration. Thus, intracellular Pt concentration could be considered as a biomarker of cisplatin resistance. In this work, an alternative method to address intrac...
The capabilities of electrospray ionization (ESI) and matrix assisted laser desorption/ionization (MALDI) to permit species identification in combination with inductively coupled plasma (ICP) mass spectrometry to conduct element-tagged species monitoring have been exploited for a number of applications in speciation studies over the years. In this...
Cisplatin, one of the most extensively used metallodrugs in cancer treatment, presents the important drawback of patient resistance. This resistance is the consequence of different processes including those preventing DNA adducts formation, and/or their quick removal. Thus, an accurate detection and quantitation of cisplatin-induced adducts might b...
Oxidative transformation of cysteine thiol groups into different functional groups is considered a significant posttranslational modification of great importance in pathological and physiological processes. A cysteine sulfenic acid (SA) residue is the transient state for thiol group oxidation and it can react with free thiols to form disulfide bond...
The use of Pt-containing compounds as chemotherapeutic agents facilitates drug monitoring by using highly sensitive elemental techniques like inductively coupled plasma mass spectrometry (ICP-MS). However, methodological problems arise when trying to compare different experiments due to the high variability of biological parameters. In this work we...
In the present work we evaluate the potential as chemotherapeutic agents of two groups of new Ru(II) complexes containing water soluble phosphane ligands and either a tris(pyrazol-1-yl)borate (Tp) or a pyridine bis(oxazoline) (pybox) ligand and their interactions with DNA. Since the interaction of these drugs can be diverse (from binding to DNA nuc...