Mario A Cleves

• Ph.D.
• Professor (Full) at University of South Florida

Appendices for the Montelukast and Neuropsychiatric Adverse Events manuscript

Genes, including those with transgenerational effects, work in concert with behavioral, environmental, and social factors via complex biological networks to determine human health. Understanding complex relationships between causal factors underlying human health is an essential step towards deciphering biological mechanisms. We propose a new analy...

Objectives Our aim was to study the association of clinical variables obtainable before delivery for severe neonatal outcomes (SNO) and develop a clinical tool to calculate the prediction probability of SNO in preterm prelabor rupture of membranes (PPROM). Methods This was a prospective study from October 2015 to May 2018. We included singleton pre...

The purpose of this study is to investigate the relationship between inflatable penile prosthesis (IPP) infection, time of year, climate, temperature and humidity. This is a retrospective IRB-approved analysis of 211 patients at 25 institutions who underwent salvage procedure or device explant between 2001 and 2016. Patient data were compiled after...

Background Accurate and precise methods to measure of body composition in infancy and childhood are needed. Objectives This study evaluated differences and precision of three methods when compared with the four‐compartment (4C) model for estimating fat mass (FM). Methods FM of children (age 14 days to 6 years of age, N = 346) was obtained using q...

Background Maternal obesity increases offspring's obesity risk. However, studies have not often considered maternal metabolic and exercise patterns as well as paternal adiposity as potential covariates. Objective To assess the relationship between parental and newborn adiposity. Methods Participants were mother‐child pairs (n = 209) and mother‐fa...

Impact statement: Estrogens are known to regulate body composition. In addition, reactive oxygen species (ROS) produced by the action of NADPH oxidase (NOX) enzymes have been linked to obesity development. We examined development of obesity and adipose tissue injury in response to feeding "Western" diets high in fat and cholesterol in intact, ovar...

The manifestation of complex traits is influenced by gene–gene and gene–environment interactions, and the identification of multifactor interactions is an important but challenging undertaking for genetic studies. Many complex phenotypes such as disease severity are measured on an ordinal scale with more than two categories. A proportional odds mod...

Gamma band activity (30–50 Hz) plays an essential role in brain development and function, but neither the early postnatal development nor subject and environmental factors influencing this development have been reported. We documented the development of resting gamma power using high density EEG recordings obtained each month from postnatal month 2...

Diethylnitrosamine-treated male mice were assigned to 4 groups: a casein-based 35% high fat ethanol liquid diet (EtOH), an EtOH diet made with soy protein isolate protein (EtOH/SOY), an EtOH liquid diet supplemented with genistein (EtOH/GEN) and a chow group. EtOH feeding, final concentration 5% (v/v), continued for 16 wks. EtOH increased incidence...

Background: The benefits of breastfeeding infants are well characterized, including those on the immune system. However, determining the mechanism by which human breast milk (HBM) elicits effects on immune response requires investigation in an appropriate animal model. Objective: The primary aim of this study was to develop a novel porcine model...

Obstructive heart defects (OHDs) are a major health concern worldwide. The platelet-derived growth factor (PDGF) genes are known to have regulatory functions that are essential for proper heart development. In a zebrafish model, Pdgfra was further demonstrated to interact with ethanol during craniofacial development. In this article, we investigate...

Purpose: Many providers elect to use a transcorporeal approach for artificial urinary sphincter placement in an attempt to minimize risks, given the increased risk of complications in revision cases. We present outcomes in a multicenter retrospective analysis of artificial urinary sphincter cuff reimplantation in patients with prior cuff erosion wi...

Objective: To investigate patients for positive culture rates with or without IRC PPs and to examine changes in culture positive isolates found in patients presenting overt clinical infection. Methods: Cultures were obtained from PPs immediately upon surgical exposure of the pump. 236 patients were broken down into 2 groups, with each further di...

Background: During the postnatal feeding period, formula-fed infants have higher cholesterol synthesis rates and lower circulating cholesterol concentrations than their breastfed counterparts. Although this disparity has been attributed to the uniformly low dietary cholesterol content of typical infant formulas, little is known of the underlying m...

Background: Understanding the role of home food environment on gestational weight gain (GWG) may provide a useful weight management strategy to help reduce excessive GWG. Materials and methods: Pregnant women recruited between 2011 and 2014 (N = 165; normal weight N = 65, overweight N = 62, obese N = 38) in Arkansas completed measures of high-fa...

Purpose: Substantial controversy and conflicting data exist regarding device survival of the artificial urinary sphincter (AUS) in patients with prior radiation therapy. Herein we present data from a multi-institutional analysis examining the effect of prior radiation for prostate cancer on device survival. Materials and methods: A database was...

Background and purpose: Studies on infants and children born preterm have shown that adequate gestational length is critical for brain white matter development. Less is known regarding how variations in gestational age at birth in term infants and children affect white matter development, which was evaluated in this study. Materials and methods:...

Objectives/hypothesis: To delineate clinical characteristics and treatment outcomes of eosinophilic esophagitis (EoE) in the youngest of children. Study design: Retrospective chart review. Methods: A 7-year retrospective chart review of children with clinicopathologic diagnosis of EoE was performed with specific analysis of patients under 5 ye...

Background: Few instruments capture symptoms that predict cardiac events in the short-term. This study examines the ability of the McSweeney Acute and Prodromal Myocardial Infarction Symptom Survey to predict acute cardiac events within 3 months of administration and to identify the prodromal symptoms most associated with short-term risk in women...

Introduction: Maternal obesity (OB) and excessive gestational weight gain (GWG) are strong independent contributors that augment obesity risk in offspring. However, direct evidence of epigenetic changes associated with maternal habitus remains sparse. Methods: We utilized Bisulfite Amplicon Sequencing (BSAS) to conduct targeted DNA methylation a...

Background: Propranolol's mechanism of action for controlling infantile hemangioma (IH) remains unclear. We hypothesize that this non-selective beta antagonist downregulates Renin-Angiotensin-Aldosterone (RAA) axis components, preventing angiogenic substrate induction of IH. Methods: IH tissue and serum were collected from children with proprano...

Maternal obesity has been shown to be associated with childhood obesity risk in both experimental and epidemiological studies. However, longitudinal studies carefully evaluating other contributors to fetal and infant body fat accretion are lacking. The objective of this study was to evaluate the effect of maternal adiposity on fetal fat mass accret...

Gamma band activity (30–50 Hz) is a significant EEG component related to intelligence, memory and language processes, but there is limited information regarding the early development of this activity and none considering how infant diet may influence this development. The present study examined changes in gamma power from 3 months through 5 years i...

Context: It is hypothesized that obesity adversely affects the ovarian environment which can disrupt oocyte maturation and may further impact embryonic development. Objective: This study aimed to compare oocyte gene expression profiles and follicular fluid (FF) content from overweight/obese (OW) women and normal weight (NW) women who were underg...

Objectives Excessive gestational weight gain (GWG) is a key modifiable risk factor for negative maternal and child health. We examined the efficacy of a behavioral intervention in preventing excessive GWG. Methods 230 pregnant women (87.4 % Caucasian, mean age = 29.2 years; second parity) participated in the longitudinal Glowing study (clinicaltria...

Objective: To evaluate the influence of both repair type and degree of cuff erosion on post-operative urethral stricture rate. Sparse literature exists regarding patient outcomes after artificial urinary sphincter (AUS) cuff erosion. Surgeons from six high-volume male continence centers compiled a comprehensive database of post-erosion patients to...

Objective: To examine pregnant women's gestational weight gain expectations and advice from various sources (ie, self, family and friends, physician) and the association of these sources of expectations and advice with measured gestational weight gain. Methods: This is a secondary analysis of a cohort study of 230 pregnant women in their second...

Background An accurate estimate of preconception weight is necessary for providing a gestational weight gain range based on the Institute of Medicine’s guidelines; however, an accurate and proximal preconception weight is not available for most women. We examined the validity of first trimester weights for estimating preconception body mass index c...

Background: We previously reported that dietary intake of shiitake mushroom (SM; Lentinus edodes) decreased serum concentrations of polar lipids in male rats. Objective: This study evaluated the dietary effects of SM on serum cholesterol-related and serum antioxidant indexes in rats of both sexes. Methods: Sprague-Dawley rats [38 dams and thei...

Since maturational processes triggering increased attunement to native language features in early infancy are sensitive to dietary factors, infant-diet related differences in brain processing of native-language speech stimuli might indicate variations in the onset of this tuning process. We measured cortical responses (ERPs) to syllables in 4 and 5...

This data file describes the bioinformatics analysis of uterine RNA-seq data comparing genome wide effects of feeding soy protein isolate compared to casein to ovariectomized female rats age 64 days relative to treatment of casein fed rats with 5μg/kg/d estradiol and relative to rats treated with estradiol and also fed soy protein isolate. Complete...

Family-based association studies are commonly used in genetic research because they can be robust to population stratification (PS). Recent advances in high-throughput genotyping technologies have produced a massive amount of genomic data in family-based studies. However, current family-based association tests are mainly focused on evaluating indiv...

Several studies have shown that the development and function of the neonatal immune system is enhanced by breast feeding compared to formula feeding. It is important to understand the interaction between infant feeding regimens and the developing GI tract, to identify the protective components of breast milk and mechanisms attributed in immune syst...

Semanticrelationships between images and words essential for language comprehension andcognitive function begin to emerge in toddlers, but it is not known if theearly development of these relationships is modulated by infant diet andgender. In this study 254 healthy 3yr‐olds [breast‐fed (BF: 86, 42 boys), or fed milk formula (MF: 88, 40 boys),or so...

In this study, diethylnitrosamine-treated male mice were assigned to 3 groups: a 35% high fat ethanol liquid diet (EtOH) with casein as the protein source, the same EtOH liquid diet with soy protein isolate as the sole protein source (EtOH/SPI) and a chow group. EtOH feeding continued for 16 wks. As expected, EtOH increased the incidence and multip...

Background Breastfeeding is associated with a variety of positive health outcomes in children and is recommended exclusively for the first 6 months of life; however, 50–70 % of infants in the US are formula-fed. To test the hypothesis that immune system development and function in neonates and infants are significantly influenced by diet, 2-day old...

There are concerns regarding reproductive toxicity from consumption of soy foods, including an increased risk of endometriosis and endometrial cancer, as a result of phytoestrogen consumption. In this study, female rats were fed AIN93G diets made with casein (CAS) or soy protein isolate (SPI) from postnatal day (PND) 30, ovariectomized on PND 50 an...

More than 80 million Americans have hypertension (HTN), and African Americans (AAs) are disproportionately affected. AAs also have lower rates of adherence to HTN treatment. It is important to understand AAs? perceptions of adherence to develop effective interventions. The aim of this study is to examine AAs? perceptions of adherence to medications...

Background and purpose: Infant diets may have significant impact on brain development in children. The aim of this study was to evaluate brain gray matter structure and function in 8-year-old children who were predominantly breastfed or fed cow's milk formula as infants. Materials and methods: Forty-two healthy children (breastfed: n = 22, 10 bo...

Congenital heart defects (CHDs) are among the most prevalent and serious birth defects, occurring in 8 to 10 of every 1000 live births in the United States. Epidemiologic studies have reported an association between CHDs and maternal smoking, but it remains unknown how genes impact the susceptibility of offspring to CHDs in the presence of maternal...

The longitudinal trajectories of body composition of children born to mothers with normal weight, overweight, and obesity have not been evaluated using precise body composition methods. This study investigated the relationship between maternal prepregnancy BMI and offspring body composition trajectories during the first 6 years of life. Healthy inf...

Right-sided and left-sided obstructive heart defects (OHDs) are subtypes of congenital heart defects, in which the heart valves, arteries, or veins are abnormally narrow or blocked. Previous studies have suggested that the development of OHDs involved a complex interplay between genetic variants and maternal factors. Using the data from 569 OHD cas...

PurposeTo compare brain gray and white matter development in healthy normal weight and obese children.Methods Twenty-four healthy 8- to 10-year-old children whose body mass index was either <75th percentile (normal weight) or >95th percentile (obese) completed an MRI examination which included T1-weighted three-dimensional structural imaging and di...

Several published reports suggest that phytochemicals, such as isoflavones found in soybeans, impair reproductive function in animals and raise the possibility that consuming soy infant formula could alter estrogen‐sensitive organ development in children. This study compared reproductive organs volumes and morphological characteristics in 101 child...

Literature reports suggest that phytochemicals, such as isoflavones found in soybeans, impair reproductive function in animals and raise the possibility that consuming soy infant formula could alter hormonally sensitive organ development in children. This study compared reproductive organs volumes and structural characteristics in children at age 5...

Family-based study design is commonly used in genetic research. It has many ideal features, including being robust to population stratification (PS). With the advance of high-throughput technologies and ever-decreasing genotyping cost, it has become common for family studies to examine a large number of variants for their associations with disease...

Postnatal nutrition influences neurodevelopment, but it is not known whether the development of individual differences in physiologic measures is related to variations in early postnatal diet. To address this issue we studied the stability of vagal tone (V)-an index of individual differences in parasympathetic heart rate control-by measuring restin...

PurposeThere is conflicting evidence regarding the association between montelukast and neuropsychiatric events (NE). We sought to examine this association among children with asthma.Methods Using a 10% sample of the LifeLink Health Plan Claims data, subjects less than 18 years of age with a primary diagnosis of asthma between 1 January 1998 and 31...

Background: Pedigree development, family history, and genetic testing are thought to be useful in improving outcomes of chronic illnesses such as hypertension (HTN). However, the clinical utility of pedigree development is still unknown. Further, little is known about the perceptions of African Americans (AAs) of family history and genetic testing....

Conotruncal heart defects (CTDs) are among the most severe birth defects worldwide. Studies of CTDs indicate both lifestyle behaviors and genetic variation contribute to the risk of CTDs. Based on a hybrid design using data from 616 case-parental and 1645 control-parental triads recruited for the National Birth Defects Prevention Study between 1997...

the development of congenital heart defects (CHDs) involves a complex interplay between genetic variants, epigenetic variants, and environmental exposures. Previous studies have suggested that susceptibility to CHDs is associated with maternal genotypes, fetal genotypes, and maternal–fetal genotype (MFG) interactions. We conducted a haplotype-based...

Background: The American Academy of Pediatrics recommends breastfeeding, which is well known to promote cognitive and behavioral development. The evidence for why this occurs is not well understood. Methods: Fifty-six 7.5- to 8.5-y-old healthy children were breastfed (BF; n = 22, 10 males) or formula-fed (FF; n = 34, 16 males) as infants. All ch...

Unlabelled: It is not currently possible to predict the probability of whether a woman with a chlamydial genital infection will develop pelvic inflammatory disease (PID). To determine if specific biomarkers may be associated with distinct chlamydial pathotypes, we utilized two Chlamydia muridarum variants (C. muridarum Var001 [CmVar001] and CmVar0...

Although alcohol effects within the liver have been extensively studied, the complex mechanisms by which alcohol causes liver cancer are not well understood. It has been suggested that ethanol (EtOH) metabolism promotes tumor growth by increasing hepatocyte proliferation. In this study, we developed a mouse model of tumor promotion by chronic EtOH...

Background: Conotruncal heart defects (CTDs) are among the most severe birth defects worldwide and result in significant morbidity and mortality. Folate-dependent methionine and homocystine metabolism is thought to play a role in such defects. To identify genes associated with CTD risk, we conducted a case-control study of single nucleotide polymor...

Nonsyndromic congenital heart defects (CHDs) develop during embryogenesis as a result of a complex interplay between environmental exposures, genetics, and epigenetic causes. Genetic factors associated with CHDs may be attributed to either independent effects of maternal or fetal genes, or the intergenerational interactions between maternal and fet...

Studies comparing child cognitive development and brain activity during cognitive functions between children who were fed breast milk (BF), milk formula (MF), or soy formula (SF) have not been reported. We recorded event‐related scalp potentials reflecting semantic processing (N400 ERP) from 20 homologous hemispheric sites in 8 yr old children [BF...

Isoflavones are phytochemical components of soy diets that bind weakly to estrogen receptors (ERs). To study potential estrogen‐like actions of soy in the mammary gland, we fed weanling male and female Sprague‐Dawley rats a casein diet from PND21 to PND33, the same diet substituting soy protein isolate (SPI) for casein, or the casein diet supplemen...

Alcohol abuse is associated with the development of fatty liver disease and also with significant osteopenia in both genders. In this study, we examined ethanol-induced pathology in response to diets with differing fat/carbohydrate ratios. Male Sprague-Dawley rats were fed intragastrically with isocaloric liquid diets. Dietary fat content was eithe...

Birth defects are a leading cause of infant morbidity and mortality worldwide. The vast majority of birth defects are nonsyndromic, and although their etiologies remain mostly unknown, evidence supports the hypothesis that they result from the complex interaction of genetic, epigenetic, environmental, and lifestyle factors. Since our last review pu...

We investigated the association between conotruncal heart defects (CTDs) and maternal and fetal single nucleotide polymorphisms (SNPs) in 60 genes in the folate, homocysteine, and transsulfuration pathways. We also investigated whether periconceptional maternal folic acid supplementation modified associations between CTDs and SNPs METHODS: Particip...

Increased availability and usage of ultrasound screening have led to improved identification of fetal structural abnormalities prenatally. Few population-based studies have been published on prenatal detection for structural birth defects in the United States. The aim of this study is to determine the frequency of maternal reporting of abnormal pre...

Introduction The primary concern for many prosthetic urologic surgeons in placing the three-piece inflatable penile prosthesis (IPP) is the concept of “blind reservoir placement.” Extensive reports permeate the literature regarding bladder, bowel, vascular, and various hernial complications occurring while attempting to place the reservoir into the...

More than 240 000 women in the United States die of coronary heart disease annually. Identifying women's symptoms that predict a coronary heart disease event such as myocardial infarction (MI) could decrease mortality. For this longitudinal observational study, we recruited 1097 women, who were either clinician referred or self-referred to a cardio...

Relationships between early postnatal diet and the development of cardiac regulation were studied using resting vagal tone and heart period measures obtained quarterly during infancy and at 2years in 158 breast-fed, 159 milk formula-fed, and 148 soy formula-fed infants. Both measures increased across time for all groups. Heart period was greater in...

Isoflavones are phytochemical components of soy diets that bind weakly to estrogen receptors (ERs). To study potential estrogen-like actions of soy in the mammary gland during early development, we fed weanling male and female Sprague-Dawley rats a semi-purified diet with casein as the sole protein source from PND21 to PND33, the casein diet supple...

Objectives: We examined variations in children's risk of an unintentional or intentional fatal injury following an allegation of physical abuse, neglect, or other maltreatment. Methods: We linked records of 514 232 children born in California from 1999 to 2006 and referred to child protective services for maltreatment to vital birth and death da...

In order to characterize the actions of xenoestrogens, it is essential to possess a solid portrait of the physiological effects of exogenous estradiol. We assessed effects of three doses of exogenous estradiol (E2) (0.1, 1.0 and 10 µg/kg/day) given between postnatal days 21 and 33 on the mammary gland morphology and gene expression profiles of male...

This study characterized total body fat mass (FM) and motor development during the first 2 years of life in healthy infants. Participants (n = 469) from the Beginnings Study cohort, a prospective, longitudinal study of early infant feeding, were assessed at 3, 6, 9, 12, and 24 months of age for a total of 2231 observations. Growth was evaluated usi...

This paper proposes that a certain premorbid personality type – that of hard driving, achievement-oriented, often exercise-oriented individuals – correlates with bupropion response; conversely, patients without these premorbid traits and whose depression is marked by mood swings, irritability and rumination are likely fluoxetine responders. The au...

To determine if dietary fat type influences nonalcoholic fatty liver disease (NAFLD), male SD rats were overfed low (5%) or high (70%) fat diets containing olive oil (OO), corn oil (CO) or echium oil (EO) using total enteral nutrition (TEN) for 21 d. Overfeeding 5% CO or 5% EO diets resulted in less steatosis than 5% OO (P< 0.05). Significant diffe...

To determine if there was a beneficial effect of dietary medium chain triglycerides (MCT) on nonalcoholic fatty liver disease (NAFLD), male rats were isocalorically overfed diets containing 10–70% total energy as corn oil or a 70% fat diet in which corn oil was replaced with increasing concentrations of saturated fat (18:82, beef tallow:MCT oil) fr...

The parasympathetic nervous system (PS) influences are critical in the autonomic control of the heart. To examine how early postnatal diet affects PS development, we used a measure of tonic PS control of cardiac activity (vagal tone) derived from resting heart rate recordings in 158 breastfed (BF), 159 milk formula‐fed (MF) and 148 soy formula‐fed...

Maternal programming of fetal metabolism has been demonstrated in animal studies, while clinical studies have shown an association between pre‐pregnancy body mass index (BMI) and anthropometric measures in infants. Here we report on the association between infant body composition at age 2 weeks and parental total body fat mass (FM) measured during...

The current study was designed to determine if the NADPH-oxidase NOX2 plays a role in development of obesity after high fat feeding. Wild type (WT) mice and mice lacking the essential cytosolic NOX2 system component p47phox (P47KO mice) were fed AIN-93G diets or high fat diets (HFD) containing 45% fat and 0.5% cholesterol for 13 weeks from weaning....

Objective: To characterize growth, fat mass (FM), fat-free mass (FFM), and bone mineral content (BMC) longitudinally in healthy infants fed breast milk (BM), cow's milk formula (CMF), or soy formula (SF) during the first year of life. Study design: Infants were assessed at age 3, 6, 9, and 12 months. Growth was evaluated using standard anthropom...

Metabolic syndrome is often accompanied by development of hepatic steatosis and less frequently by non-alcoholic fatty liver disease (NAFLD) leading to non-alcoholic steatohepatitis (NASH). Replacement of corn oil with medium chain triacylglycerols (MCT) in the diets of alcohol-fed rats has been shown to protect against steatosis and alcoholic live...

Anthropometrics and body mass index are only proxies in the evaluation of adiposity in the pediatric population. Air displacement plethysmography technology was not available for children aged 6 months to 9 years until recently. Our study was designed to test the precision of air displacement plethysmography (ADP) in measuring body fat mass in chil...

Background: Studies have examined the association between acetaminophen use and asthma; however, their interpretation is limited by several methodologic issues. Objective: To investigate the association between recent and chronic prescription-acquired acetaminophen use and asthma. Methods: This retrospective case-control study used a 10% rando...

Background: Coronary heart disease (CHD) mortality rates are higher among women, particularly black, than men. Women's mortality rates may reflect difficulty in recognizing CHD prodromal symptoms (PS) but reliable screening instruments for women are scarce. The McSweeney Acute and Prodromal Myocardial Infarction Symptom Survey (MAPMISS) captures w...

Background: The development of non-syndromic congenital heart defects (CHDs) involves a complex interplay of genetics, metabolism, and lifestyle. Previous studies have implicated maternal single nucleotide polymorphisms (SNPs) and altered metabolism in folate-related pathways as CHD risk factors. Objective: We sought to discover associations bet...

To determine if dietary fat composition affects the progression of nonalcoholic fatty liver disease (NAFLD), male Sprague-Dawley rats were overfed low (5%) or high (70%) fat diets with different fat sources: olive oil (OO), corn oil (CO) or echium oil (EO) using total enteral nutrition (TEN) for 21 d. Overfeeding of the 5% CO diet resulted in less...

The leading patient complaint during the perioperative period for penile prosthesis implantation is postoperative pain, while emesis and urticaria also affect the procedure's perceived success. In analyzing surgical outcomes, assessment of the anesthetic for postoperative pain and side effects should be included. This paper retrospectively reviews...

The study objective was to describe the prevalence and correlates of sleep disturbances among women who retrospectively reported sleep disturbance before their myocardial infarction (MI). MI is frequently unrecognized in women because they may have only vague symptoms, such as sleep disturbance. Describing correlates of sleep disturbance before MI...

Although soy formula has been reported to support normal development, concerns exist regarding potential adverse developmental effects of phytochemicals associated with soy protein. This study characterized developmental status (mental, motor, and language) of breastfed (BF), milk-based formula-fed (MF), or soy protein-based formula-fed (SF) infant...