Marina A G von Keyserlingk

University of British Columbia | UBC · Faculty of Land and Food Systems, Animal Welfare Program

In this first chapter, we summarize how the science and our thoughts have progressed since the publication of The Welfare of Cattle, (a predecessor to the present volume) 15 years ago. We discuss some of the advances made on cattle welfare issues over the last decade and also highlight areas where more work is needed. We also emphasize the importan...

Social buffering occurs when the presence of a partner mitigates the stress response of an individual. In two experiments, we assessed the effects of social buffering in dairy calves, with and without a known conspecific, when either subjected to isolation from the larger group (Experiment 1; n = 12) or to recovery from the painful procedure of hot...

In humans, early painful experiences can increase pain sensitivity later in life, but little is known regarding this phenomenon in cattle. This study assessed if a painful event early in life affects later pain sensitivity in 40-d old calves. Holstein calves (n = 26) were randomly assigned to control or treatment conditions, blocked within sex and...

Veterinarians and animal scientists can provide leadership on issues relevant to farm animal welfare, but perceptions of these stakeholders regarding societal expectations for welfare are underexplored. This study involved five focus groups of veterinarians and animal scientists (n = 50 in total), recruited at a European meeting focused on cattle w...

Negative emotional states are known to interact, potentially aggravating one another. In this study, we used a well validated paradigm (successive negative contrast, SNC) to determine if pain from a common procedure (disbudding) influences responses to a reward downshift. Holstein calves (n = 30) were trained to approach a 0.5 L milk reward. Latenc...

Simple Summary A qualitative study was conducted using an online survey platform with participants asked to provide their views on one of three scenarios, that differed in the amount of information, describing a farmer administering a proven antibiotic to a sick cow. While many respondents appeared to be supportive of the farmer’s decision to treat...

Dairy cattle typically live in groups, but individuals within these groups vary in their social behavior. An improved understanding of factors affecting the expression of social behavior may help refine management practices on farms to better accommodate the needs of all individuals within the herd. In this paper, we review 1) some examples of how...

Studies have found evidence of pain in the hours following hot-iron disbudding, but little is known about longer lasting pain following this procedure. Work on humans and rats has shown that lasting pain can have negative effects on the formation and recall of memories. The objective of this study was to assess whether lasting pain following disbud...

The contentious issue of cow calf separation at birth is incongruent with many views on acceptable farming practices, and carries the risk of eroding public trust in the dairy industry if it is not addressed. The available evidence provides little support for the practice, but research on best practices for maintaining cow calf contact in a way tha...

Genetic engineering of animals has been proposed to address societal problems, but public acceptance of the use of this technology is unclear. Previous work has shown that the source of information proposing the technology (e.g. companies, universities), the term used to describe the technology (e.g. genome editing, genetic modification), and the g...

On most dairy farms, calves are housed individually until weaning. However, depriving calves of an early social environment impairs behavioral development. We studied the effect of early-life social housing on calves' competitive skills. In this study, Holstein heifers were pseudorandomly assigned to either individual housing (n = 9) or pair housin...

Lameness assessments are rarely conducted routinely on dairy farms and when completed typically underestimate lameness prevalence, hampering early diagnosis and treatment. A well-known feature of many perceptual tasks is that relative assessments are more accurate than absolute assessments, suggesting that creating methods that allow for the relati...

Dairy cows are usually culled and transported from the farm when they no longer meet the farm’s standards for production or are not needed for milk production. Some cows are transported while in poor condition and may deteriorate further during transport. In February 2020, Canadian federal animal transport regulations were revised with the aim to m...

Concerns regarding the welfare of farm animals continue to grow. Traditionally, research efforts have largely focused on refining existing management practices to improve welfare. However, the incorporation of views from those directly involved in animal care is equally, if not more, important. This study investigated the perspectives of Canadian d...

In humans, the ‘peak-end’ rule states that recollection of an experience is most often influenced by the peak (the most intense moment) and end of the experience. We investigated whether calves followed the peak-end rule in their memory of a painful procedure: disbudding. As proxies for retrospective and ‘real-time’ reports of pain, we used conditi...

Surplus dairy calves and cull cows are often transported from the dairy farm to a variety of destinations and may experience compromised health and welfare in the process. Increasing interest in farm animal welfare by many stakeholders, including the public and policymakers, has brought about recent changes to livestock transport regulations in Can...

Simple Summary Indoor housed dairy cows are highly motivated to scratch themselves using mechanical brushes. Many farms provide brushes to cows, yet no commercial brushes to date capture how the brushes are used by the cows in the pen. We developed an automated brush and tested how much cows use it at four different group sizes (60, 48, 36, and 24...

Dairy calf welfare is a growing interest within the veterinary field. However, a limited understanding of the conception of calf welfare by dairy cattle veterinarians can hinder efforts to promote welfare improvements on farms. The aim of this study was to explore how focus groups can promote learning about dairy calf welfare issues among cattle ve...

Hunger remains a significant animal welfare concern as restricted feeding practices are common on farms. Studies to date have focused on negative effects on health and productivity but little research has addressed the feeling of hunger, mostly due to methodological difficulties in assessing animals' subjective experiences. Here, we explored the us...

Cattle are gregarious animals able to form social relationships. Dominance is one of the most widely studied social behaviors of dairy cattle, especially cows confined indoors. However, much of the past dairy cattle research has used an unstandardized approach, differing in definitions and conceptual understanding of dominance, as well as their met...

Male cattle reared for beef are typically castrated. Male calves born on dairy farms are increasingly reared for beef, so a better understanding of how to humanely perform this procedure in dairy systems is now required. We studied the short-term affective responses of dairy calves to castration using a conditioned place aversion paradigm. Young Ho...

Current systems for managing surplus dairy calves are wrought with ethical and animal welfare concerns. Resolving complex problems in the dairy industry requires engagement from dairy farmers and other stakeholders. The main objective of this case study was to pilot a novel methodology to deepen our understanding of how dairy producers envision the...

The objective of this study was to assess the effects of intake-based weaning methods and forage type on feeding behavior and growth of dairy calves. Holstein dairy calves (n = 108), housed in 12 groups of 9, were randomly assigned to 1 of 3 weaning treatments: milk reduction based on age (wean-by-age), individual dry matter intake (DMI; wean-by-in...

There is growing interest in gene editing farm animals. Some alterations could benefit animal welfare (e.g., improved heat tolerance in cattle with the “slick” gene), the environment (e.g., reducing methane emissions from cattle with induced pluripotent stem cells), and productivity (e.g., higher weight gains in cattle with the “double muscling” ge...

Ethical and regulatory oversight of research animals is focused on vertebrates and rarely includes invertebrates. Our aim was to undertake the first study to describe differences in public confidence, trust, and expectations for the oversight of scientists using animals in research. Participants were presented with one of four treatments using a 2...

Both male and female calves that are not required in the dairy herd sometimes receive inadequate care on dairy farms. Veterinarians work with farmers to improve animal care, and farmers often view veterinarians as trusted advisors; however, little is known about the attitudes of veterinarians on surplus calves. This study investigated the perspecti...

Lameness assessments are commonly conducted at a single point in time, but such assessments are subject to multiple sources of error. We conducted a longitudinal study, assessing the gait of 282 lactating dairy cows weekly during the first 12 wk of lactation, with the aim of assessing how lameness prevalence changed in relation to case definition a...

Lameness is a common condition in dairy cows. Free-choice access to pasture may benefit lame cows by providing a softer and more comfortable lying and standing surface; however, the effects of this system on lameness have not yet been explored. We evaluated whether a 7-wk period of free-choice pasture access would improve lameness recovery and affe...

Temperature variability resulting from climate change poses challenges around the world for livestock production and the welfare of the animals in these systems. As animal industries attempt to combat these challenges, it is vital to understand how potential changes implemented by farmers resonate with societal values. The aims of this study were t...

As awareness grows, some traditional management practices used by the dairy industry will be questioned by members of the public. Therefore, to maintain its social license to operate, the industry needs to account for public perspectives when developing future directions. Our aims were to assess attitudes of members of the public toward the managem...

Transition period (±3 wk around calving) diseases are prevalent in dairy systems. In this review we describe the physiological and behavioral changes experienced by cows during the transition period and during the dry period leading up to this. Our narrative review examines risk factors associated with these diseases in zero-grazing and grazing sys...

The hole-board test has been used to assess working and reference memory in a variety of species, but not in cattle. We developed and applied a modified hole-board test for dairy calves. Fifteen bottles were evenly spaced along three walls of a test arena; 11 of these were empty and 4 were ‘baited’ with milk. Calves were exposed daily (over an 11-d...

Public opinion can affect the adoption of genome editing technologies. In food production, genome editing can be applied to a wide range of applications, in different species and with different purposes. This study analyzed how the public responds to five different applications of genome editing, varying the species involved and the proposed purpos...

The provision of pasture and outdoor access for dairy cattle differs around the globe. For example, in Ireland, New Zealand, and Australia, dairy farms are largely pasture based, whereas dairy farms in the United States and Canada are largely confinement based. There is a high level of public support for pasture and outdoor access for dairy cows, a...

Early cow-calf separation followed by individual housing of calves is standard practice on dairy farms. However, a growing body of evidence suggests that as awareness grows the public will oppose these practices, which could compromise the dairy industry's social license. Despite disagreement among different stakeholders over weighting and evaluati...

Calf rearing practices differ among farms, including feeding and weaning methods. These differences may relate to how dairy producers view these practices and evaluate their own success. The aim of this study was to investigate perspectives of dairy producers on calf rearing, focusing on calf weaning and how they characterized weaning success. We i...

Adult dairy cattle show a preference for outdoor spaces during summer nights, but little is known about such preferences for dairy calves. Our aim was to determine the preferences of dairy calves for outdoor access during their first 11 wk of age in summer conditions. Calves were paired (n = 10) at 7 d of age and placed in one of 10 pens (7.32 × 2....

An animal’s social position within a group can influence its ability to perform important behaviours like eating and resting, but little is known about how social position affects the ability to express what are arguably less important but still rewarding behaviors, such as grooming. We set out to assess if dominance measured at the feeder is assoc...

Most dairy calves are housed individually in the first weeks and sometimes months of their lives. Lack of social interaction can negatively impact feed intake, social skills, coping abilities, and cognitive performance, but the motivation of calves to seek companionship has seldom been investigated. In this study, 10 Holstein bull calves (Bos tauru...

The objective of this study was to assess the motivation of young calves for a social partner. Calves were presented (without prior training) with two gates they could push, giving access to either a pen with a social partner or a similar but empty pen. Every time calves pushed for one of the pens, additional weight was attached to its gate. Over 1...

Dairy cows are often subjected to multiple post-partum stressors but how these stressors impact cows’ affective states remain poorly understood. Negative affective states are often associated with reduced expression of low-resilience behaviors, so we explored whether cows would reduce their use of a brush after calving. Before calving, cows were of...

Cows typically defecate while standing. Freestalls are designed to position standing cows such that their feces fall into the alley. Cows sometimes defecate while lying down, increasing the risk that feces contaminate the stall surface. We conducted 2 studies investigating cow-level and environmental factors associated with defecating while recumbe...

Dairy cattle can experience problems adjusting to freestalls when first introduced, resulting in a marked reduction in lying time and increased abnormal behaviors such as lying in the alley or lying backward in a stall. Our objective was to evaluate 2 strategies to ease the transition of heifers to freestalls: using an older, experienced heifer as...

Social referents provide information that aid in farmer decision making. Some social referents, such as veterinarians, have been well studied in the context of dairy farms, but others have not and the role of social referents may change across management practices. This study aimed to understand (1) who farmers consult when making management decisi...

Many male dairy calves are castrated when reared for beef production, but for dairy breeds the assessment of the longer-lasting pain associated with this procedure has received little scientific attention. In this study we assessed 2 methods: surgical (n = 10 calves) and rubber ring (n = 11). All calves were castrated at 28 d of age using multimoda...

Humans express stable differences in pessimism that render some individuals more vulnerable to stressors and mood disorders. We explored whether non-human animals express stable individual differences in expectations (assessed via judgment bias tests) and whether these differences relate to susceptibility to stressors. Judgment bias tests do not di...

We used an operant conditioning paradigm to test the motivation of non-pregnant dairy heifers to access shade during the summer and autumn months (January to June) in southern Brazil. Dairy heifers (n = 18) were trained to push a weighted gate to access either an experimental area containing both a shaded (simple tree shade and shade cloth) and uns...

One important type of animal welfare concern is “natural living” (i.e., that animals are able to express natural behaviours that are important to them, and to engage with aspects of the natural world that they find important). The aims of this narrative review were to describe the behavioural development of calves (Bos taurus) in natural settings a...

Many captive Asian elephants (Elephas maximus) in Thailand participate in the tourism industry at attractions known as 'elephant camps.' There has been significant criticism of low welfare venues, where the elephants may experience injuries, poor nutrition, unnatural social environments and aversive handling. Despite increasing concern for animal w...

Weaned dairy heifers are often housed in environments with few appropriate outlets for grooming or oral manipulation. Our objective was to characterize brush use by naive heifers, including patterns over time. In phase 1, groups of 4 heifers (n = 13 groups, 146.4 ± 9.1 d old, mean ± standard deviation; SD) were introduced to a bedded pack pen with...

Dairy cows are highly motivated to access pasture, especially at night in summer. When pasture is not available, dairy cows show a partial preference for alternative types of outdoor access, spending half the night outside in summer on an outdoor sand or wood chip pack. However, many dairy farms do not provide cows outside access. To better underst...

Many dairy cattle worldwide are housed in tiestalls, meaning that they are tethered by the neck to individual stalls. On some farms, tied cattle are permitted seasonal access to pasture, but otherwise their movements are restricted compared with cows housed in freestall barns or other loose housing systems. The aim of this systematic review is to s...

Studies performed on individual research farms have reported that dairy cattle developing sole hemorrhages or sole ulcers in peak to mid lactation spent more time standing during the weeks around calving. The aim of this prospective observational longitudinal study was to evaluate whether this relationship is evident in commercial dairy herds. A co...

Small, tropical fish are popular companion animals and constitute a major proportion of the vertebrates used in scientific research, but little is known about how they are affected by routine husbandry practices. Manual tank cleanings are a common and potentially stressful maintenance procedure that involves siphoning out a substantial volume of ta...

The practice of rearing cows and calves together is gaining popularity on dairy farms, with different systems currently under assessment in mainland Europe, the United Kingdom, and Oceania. Research into the effects of cow–calf rearing has primarily focused on direct health and welfare implications, and little work has examined the role of differen...

Modern freestall barns for dairy cattle have been constructed with considerations for dairy cow cleanliness; partitions and other stall features such as neck rails are designed to reduce manure contamination of bedding and decrease farm labor. However, cows prefer to lie in more open spaces, including on bedded packs and pasture. We created an “alt...

Interventions that aim to help farmers change on-farm practices recommend that advisors communicate effectively with farmers, work collaboratively to set goals and provide farmers with resources that are applicable to the farm context. We developed an intervention that aimed to help farmers modify and use a standard operating procedure (SOP) for co...

Integrating technology into agricultural systems has gained considerable traction, particularly over the last half century. Agricultural systems that incorporate the public’s concerns regarding farm animal welfare are more likely to be socially accepted in the long term, a key but often forgotten component of sustainability. Gene editing is a tool...

Farm management can directly and indirectly affect animal care. We explored how farm management affected animal care on two large dairy farms in China (anonymized as Farm A and Farm B). We used a mini-ethnographic case study design whereby the first author lived for 38 days on Farm A and 23 days on Farm B. She conducted participant observation and...

Students completing advanced degrees in dairy or animal science may go on to have a major impact on the food animal agriculture industries. The aim of this study was to better understand student views of the future of dairying, including changes in practices affecting animal care on farms as well as perceived public perceptions. We conducted 6 focu...

Concern over the welfare of captive animals is growing. Captivity is often associated with frequent exposure to stressors, which may be the source of persistent negative affective states. We argue that captivity may lead to depressive-like states and call for more research on this topic. Free 50 days access here: https://authors.elsevier.com/a/1cv...

Surplus dairy calves consist of all dairy bull calves and any heifer calves not needed as replacements for the milking herd. The fate of these surplus calves varies by region; for example, in Australia and New Zealand they are often sold as “bobby” calves and slaughtered within the first weeks of life; whereas, in North America they are normally so...

Cattle are subjected to routine procedures that require restraint and close contact to humans, which are both potentially aversive to the animal. Positive reinforcement training techniques may affect how animals perceive and respond to these procedures. The objectives of the current study were to describe a positive reinforcement regimen used to tr...

At the start of the COVID-19 pandemic, Universities around the world were forced to rapidly transition from face-to-face learning environments to online learning. This paper describes this transition through the lens of a professor responsible for a third year Applied Biology class focused on providing undergraduates with research experience. The p...

This study evaluated feed intake, milk yield, and subclinical ketosis in dairy cows in early lactation fed 2 different diets postpartum. Cows are typically offered a high-energy ration immediately after calving. We compared a conventional high-energy total mixed ration (TMR) with a transition ration that contained chopped straw. We predicted that a...

Pain induces deficits in appreciation of rewards (i.e. anhedonia) and variation in response to pain may be partly explained by individual differences in general expectations (i.e. optimism). Dairy calves are routinely subjected to painful procedures such as hot-iron disbud-ding. We tested if female Holstein calves (n = 17) display signs of anhedoni...

Dairy farms are increasing in size and moving from family to external labor. As such, dairy farmers now have the responsibilities of a human resource manager in addition to caring for their animals. The objective of this paper was to review literature in 5 areas of human resource management of a dairy farm: (1) professional accreditation and profes...

The primary aim of this prospective experimental study was to evaluate how the social environment after calving influenced standing behavior in primiparous cows. At calving, primiparous cows were mixed with familiar peers in a low-stocked pen (≤33% stocking density; n = 22) or mixed with unknown older cows at 100% stocking density (n = 20). All stu...

Intensively housed dairy cattle are commonly regrouped (mixed into a new social group) as part of routine farm procedures. This stressful procedure triggers heightened levels of agonistic behaviors and disrupts animals' time budgets. However, little is known regarding the effects of regrouping on cattle's affective states. The aim of this study was...

Cauterization by hot iron and application of caustic paste are 2 common methods of disbudding calves. In this study, we compared the affective experience of these 2 procedures on young dairy calves using conditioned place aversion. Male dairy calves (n = 14; 7 ± 2 d old) were disbudded by both thermal and chemical methods (1 horn bud at a time, 48...