marie-noelle Giraud

marie-noelle Giraud
Université de Fribourg · Institut de la Cardiologie

PhD

About

80
Publications
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1,909
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Additional affiliations
January 2012 - present
Université de Fribourg
January 2008 - December 2009
January 2007 - December 2012
Inselspital, Universitätsspital Bern

Publications

Publications (80)
Article
Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main funding source(s): The study was supported by the Swiss National Science Foundation (SNF 310030_149986) attributed to MNG, the University of Fribourg and the Fonds Scientifique Cardiovasculaire FSC, Fribourg Hospital attributed to SC The spatiotemporal in...
Article
Full-text available
Cathepsin B is a lysosomal cysteine protease that plays an important role in cancer, atherosclerosis, and other inflammatory diseases. The suppression of cathepsin B can inhibit tumor growth. The overexpression of cathepsin B can be used for the imaging and photodynamic therapy (PDT) of cancer. PDT targeting of cathepsin B may have a significant po...
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To obtain meaningful results of hepatic stellate cell (HSC) function, it is crucial to use highly pure HSC populations. Our aim was to optimize HSC isolation from mice livers without exploiting the characteristically transient vitamin A autofluorescence of HSC. HSCs were isolated from C57BL/6 mice using a two-step collagenase digestion and Nycodenz...
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Interactions between macrophages, cardiac cells and the extracellular matrix are crucial for cardiac repair following myocardial infarction (MI). We hypothesized that cell-based treatments might modulate these interactions. After validating that bone marrow cells (BMC) associated with fibrin lowered the infarct extent and improved cardiac function,...
Preprint
Interactions between macrophages, cardiac cells and the extracellular matrix are crucial for cardiac repair following myocardial infarction (MI). The paracrine effects of cell-based treatments of MI might modulate these interactions and impact cardiac repair. The immunomodulatory capacity of the therapeutic cells is therefore of interest and could...
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Background The injection of mesenchymal stem cells (MSCs) mitigates fat accumulation in released rotator cuff muscle after tendon repair in rodents. Purpose To investigate whether the injection of autologous MSCs halts muscle-to-fat conversion after tendon repair in a large animal model for rotator cuff tendon release via regional effects on extra...
Article
Early-stage detection of cutaneous melanoma can vastly increase the chances of cure. Excision biopsy followed by histological examination is considered the gold standard for diagnosing the disease, but requires long high-cost processing time, and may be biased, as it involves qualitative assessment by a professional. In this paper, we present a new...
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Primary hemostasis consists in the activation of platelets, which spread on the exposed extracellular matrix at the injured vessel surface. Secondary hemostasis, the coagulation cascade, generates a fibrin clot in which activated platelets and other blood cells get trapped. Active platelet-dependent clot retraction reduces the clot volume by extrud...
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Neonatal and juvenile porcine islet cell clusters (ICC) present an unlimited source for islet xenotransplantation to treat type 1 diabetes patients. We isolated ICC from pancreata of 14 days old juvenile piglets and characterized their maturation by immunofluorescence and insulin secretion assays. Multipotent mesenchymal stromal cells derived from...
Article
Introduction Repair of the cardiac structure and function is the holy grail for curative treatment of myocardial infarction (MI). Clinical trials for cardiac cell-based therapies have revealed numerous challenges and led to a shift in the initial paradigms. The current concept is that cell/matrix-based therapy modifies the local immune response, re...
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Although the versatility of photodynamic therapy (PDT) is well established, the technical aspects of light delivery systems vary significantly depending on the targeted organ. This article describes the optical properties of a light and drug delivery system (catheter and light diffuser) suitable for intra-arterial PDT by using a planar imaging goni...
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The broad clinical use of synthetic vascular grafts for vascular diseases is limited by their thrombogenicity and low patency rate, especially for vessels with a diameter inferior to 6 mm. Alternatives such as tissue-engineered vascular grafts (TEVGs), have gained increasing interest. Among the different manufacturing approaches, 3D bioprinting pre...
Preprint
Full-text available
The broad clinical use of synthetic vascular grafts for vascular diseases is limited by their thrombogenicity and low patency rate, especially for vessels with a diameter inferior to 6 mm. Alternatives such as tissue-engineered blood grafts (TEBGs) have gained increasing interest. Among the different manufacturing approaches, 3D bioprinting present...
Data
Figure S1 Cell morphology. Before in vivo injection, cell morphology was assessed by fluorescence microscopy after in vitro staining for cell surface CD8a expression for control cells (CD8) or the two VEGF‐expressing populations (ALL and SPEC). Size bar: left column = 500 μm, right column = 50 μm.
Data
Figure S2 VEGF production. Production of rat VEGF by the injected human cells (control cells CD8; VEGF‐producing cells ALL and SPEC) after 4 weeks in vivo assessed by immunostaining for rat VEGF (rVEGF, in red), human nuclei (HuNu, in green) and nuclei by DAPI staining (blue).
Article
Full-text available
Vascular Endothelial Growth Factor (VEGF) can induce normal or aberrant angiogenesis depending on the amount secreted in the microenvironment around each cell. Towards a possible clinical translation, we developed a Fluorescence Activated Cell Sorting (FACS)-based technique to rapidly purify transduced progenitors that homogeneously express a desir...
Article
Due to the importance of peripheral blood hematopoietic stem and progenitor cells (HPCs) in post-acute regeneration after acute myocardial infarction (MI), the present study aimed to investigate count and secondary replating capacity/self-renewal ability of HPCs in peripheral blood before and two weeks after MI. In nine female Lewis inbred rats (n...
Chapter
Ischemic heart disease is the most frequent causes of death worldwide. The urgent need for new therapies fostered the development of strategies that focus on the recovery of the cardiac structure and function. Initial investigations of cell-based therapies provided evidence of beneficial outcomes such as improved heart function and reduced remodell...
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Acute coronary syndrome resulting from coronary occlusion following atherosclerotic plaque development and rupture is the leading cause of death in the industrialized world. New Zealand White (NZW) rabbits are widely used as an animal model for the study of atherosclerosis. They develop spontaneous lesions when fed with atherogenic diet; however, t...
Article
Cardiac bone marrow-derived cell-based therapy associated with scaffold for heart regeneration I. Borrego1, A. Frobert1, J. Valentin1, G. Ajalbert1, V. Roth2, B. Fellay2, S. Cook1, M.N. Giraud1. 1University of Fribourg, Department of Medicine - Cardiology, Fribourg, Switzerland; 2Laboratory HFR - Tafers, Hospital Fribourgeois, Fribourg, Switzerland...
Article
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Myocardial infarction is defined as cardiomyocyte death due to prolonged ischemia; an inflammatory response and scar formation (fibrosis) follow the ischemic injury. Following the initial acute phase, chronic remodeling of the left ventricle (LV) modifies the structure and function of the heart. Permanent coronary ligation in small animals has been...
Article
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Acute coronary syndromeis a life-threatening condition of utmost clinical importance, which, despite recent progress in the field, is still associated with high morbidity and mortality. Acute coronary syndrome results from a rupture or erosion of vulnerable atherosclerotic plaque with secondary platelet activation and thrombus formation, which lead...
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Unlabelled: Photodynamic therapy (PDT), which is based on the activation of photosensitizers with light, can be used to reduce plaque burden. We hypothesized that intra-arterial photosensitizer administration and photo-activation will lead to high and rapid accumulation within the plaque with reduced systemic adverse effects. Thus, this "intra-art...
Article
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Coronary artery ligations to induce myocardial infarction (MI) in mice and rats are widely used in preclinical investigation. However, myocardial ischemic damage and subsequent infarct size are highly variable. The lack of standardization of the model impairs the probability of effective translation to the clinic. Cardiac Troponin I (cTnI) is a maj...
Article
Coronary artery disease, the main cause of heart disease, develops as immune cells and lipids accumulate into plaques within the coronary arterial wall. As a plaque grows, the tissue layer (fibrous cap) separating it from the blood flow becomes thinner and increasingly susceptible to rupturing and causing a potentially lethal thrombosis. The stabil...
Article
We perform subsurface ablation of atherosclerotic plaque using ultrafast pulses. Excised mouse aortas containing atherosclerotic plaque were ablated with ultrafast near-infrared (NIR) laser pulses. Optical coherence tomography (OCT) was used to observe the ablation result, while the physical damage was inspected in histological sections. We charact...
Article
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Cardiac cell therapy has gained increasing interest and implantation of biomaterials associated with cells has become a major issue to optimize myocardial cell delivery. Rodent model of myocardial infarction (MI) consisting of Left Anterior Descending Artery (LAD) ligation has commonly been performed via a thoracotomy; a second open-heart surgery v...
Article
Materials for biomedical applications typically involve surface engineering. Scaffolds used for tissue engineering, for example, require a surface functionalization in order to support cell growth. The deposition of functional plasma polymer coatings seems to be an attractive approach to modify substrates for biomedical applications. Possible degra...
Article
Cardiac tissue engineering approaches can deliver large numbers of cells to the damaged myocardium and have thus increasingly been considered as a possible curative treatment to counteract the high prevalence of progressive heart failure after myocardial infarction (MI). Optimal scaffold architecture, mechanical and chemical properties, as well as...
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Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer. Here, we describe two protocols involving in vivo electroporation for gene transfer to the beating heart.
Article
Mechanical unloading of failing hearts can trigger functional recovery but results in progressive atrophy and possibly detrimental adaptation. In an unbiased approach, we examined the dynamic effects of unloading duration on molecular markers indicative of myocardial damage, hypothesizing that potential recovery may be improved by optimized unloadi...
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As opposed to culture on standard tissue-treated plastic, cell culture on three-dimensional scaffolds impedes additional challenges with respect to substrate preparation, cell seeding, culture maintenance, and analysis. We herewith present a general route for the culture of primary cells, differentiated cells, or stem cells on plasma-coated, electr...
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Engineered muscle constructs provide a promising perspective on the regeneration or substitution of irreversibly damaged skeletal muscle. However, the highly ordered structure of native muscle tissue necessitates special consideration during scaffold development. Multiple approaches to the design of anisotropically structured substrates with groove...
Article
Post-myocardial infarction pathologies are additionally compromized by the poor self-repair capacity of the myocardium and the limited possibilities for clinical interventions for regeneration. Cell therapy and in particular tissue engineering evolved as attractive therapeutic options to counteract the high mortality after myocardial infarction and...
Article
Full-text available
Vascular Endothelial Growth Factor (VEGF) can induce normal angiogenesis or the growth of angioma-like vascular tumors depending on the amount secreted by each producing cell, as it remains localized in the microenvironment. In order to control the distribution of VEGF expression levels in vivo, we recently developed a high-throughput FACS-based te...
Article
Objectives: Mechanical stimulation of engineered tissue holds great promise for the development of functionally structured tissues. In particular, mimicking the mechanical properties of native heart and the loading conditions of the cardiac tissue in vitro relies on dynamic, three dimensional culture systems that simulate the anatomical geometry an...
Article
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Cell therapies have gained increasing interest and developed in several approaches related to the treatment of damaged myocardium. The results of multiple clinical trials have already been reported, almost exclusively involving the direct injection of stem cells. It has, however, been postulated that the efficiency of injected cells could possibly...
Article
Tissue engineering has been increasingly brought to the scientific spotlight in response to the tremendous demand for regeneration, restoration or substitution of skeletal or cardiac muscle after traumatic injury, tumour ablation or myocardial infarction. In vitro generation of a highly organized and contractile muscle tissue, however, crucially de...
Article
Objective: As previously shown, myocardial unloading of a normal heart results in progressive atrophy associated with gradual loss of cardiomyocyte volume, shifted myocyte/connective tissue ratio, increase in myocardial stiffness and modifications of ventricular contractile capacity. Here, we examined effects of long-term unloading on gene expressi...
Article
Full-text available
Purpose VEGF can induce normal or aberrant angiogenesis depending exclusively on the amount secreted in the microenvironment, and not on its total dose, as it remains localized in the matrix around each producing cell. To make this concept clinically applicable we developed a FACS-based technique to rapidly purify transduced progenitors specificall...
Article
Full-text available
Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer. In the current study, we investigated the efficiency and safety of a protocol involving in vivo electroporation for gene transfer to...
Chapter
Myocardial tissue engineering ambitions to regenerate, repair or replace damaged cardiac muscle by combining cellular and engineering technologies. Several issues must be addressed before this approach may one day find clinical applications for cardiac disorders such as congenital diseases or ventricular dysfunction following myocardial infarction...
Article
Cell transplantation presents great potential for treatment of patients with severe heart failure. However, its clinical application was revealed to be more challenging than initially expected in experimental studies. Further investigations need to be undertaken to define the optimal treatment conditions. We previously reported on the epicardial im...
Article
Background: Gene therapy represents a promising strategy for the treatment of heart failure (HF). Mostly, applied vectors such as replicant free viruses, show serious adverse effects (host immune reactions, cancer development). Electroporation may present an approach to overcome these shortcomings. Given the down-regulation of the β-adrenergic rece...
Article
Recent studies have shown that mechanically unloading a failing heart may induce reverse remodeling and functional improvement. However, these benefits may be balanced by an unloading-related remodeling including myocardial atrophy that might lead to decrease in function. Using a model of heterotopic heart transplantation, we aimed to characterize...
Article
Reperfusion injury is insufficiently addressed in current clinical management of acute limb ischemia. Controlled reperfusion carries an enormous clinical potential and was tested in a new reality-driven rodent model. Acute hind-limb ischemia was induced in Wistar rats and maintained for 4 hours. Unlike previous tourniquets models, femoral vessels w...
Article
Full-text available
Tenascin-C (TNC) is a mechano-regulated, morphogenic, extracellular matrix protein that is associated with tissue remodeling. The physiological role of TNC remains unclear because transgenic mice engineered for a TNC deficiency, via a defect in TNC secretion, show no major pathologies. We hypothesized that TNC-deficient mice would demonstrate defec...
Article
Mechanisms underlying improvement of myocardial contractile function after cell therapy as well as arrhythmic side effect remain poorly understood. We hypothesised that cell therapy might affect the mechanical properties of isolated host cardiomyocytes. Two weeks after myocardial infarction (MI), rats were treated by intramyocardial myoblast inject...
Article
Tissue engineering represents an attractive approach for the treatment of congestive heart failure. The influence of the differentiation of myogenic graft for functional recovery is not defined. We engineered a biodegradable skeletal muscle graft (ESMG) tissue and investigated its functional effect after implantation on the epicardium of an infarct...
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Full-text available
METHODS: The nano- and micron-sized non- wovens were prepared by an electrospinning procedure. Therefore, the biocompatible polymer polycaprolactone (PCL) was dissolved in appropriate solvents and spun by applying a high voltage on a needle tip. Partial parallelisation of the nanofibers was obtained by using electrostatic lens systems and a fast ro...
Article
Full-text available
Myocardial tissue engineering aims to repair, replace, and regenerate damaged cardiac tissue using tissue constructs created ex vivo. This approach may one day provide a full treatment for several cardiac disorders, including congenital diseases or ventricular dysfunction after myocardial infarction. Although the ex vivo construction of a myocardiu...
Article
Intramyocardial transplantation of skeletal myoblasts augments postinfarction cardiac function. However, poor survival of injected cells limits this therapy. It is hypothesized that implantation of myoblast-based scaffolds would result in greater cell survival. Rat skeletal myoblasts were seeded on highly porous polyurethane (PU) scaffolds (7.5 x 7...
Article
Treatment of metastatic breast cancer with doxorubicin (Doxo) in combination with trastuzumab, an antibody targeting the ErbB2 receptor, results in an increased incidence of heart failure. Doxo therapy induces reactive oxygen species (ROS) and alterations of calcium homeostasis. Therefore, we hypothesized that neuregulin-1 beta (NRG), a ligand of t...
Article
Even though the mechanism is not clearly understood, direct intramyocardial cell transplantation has demonstrated potential to treat patients with severe heart failure. We previously reported on the bioengineering of myoblast-based constructs. We investigate here the functional outcome of infarcted hearts treated by implantation of myoblast-seeded...