Marie-Edith Chabouté

Marie-Edith Chabouté
French National Centre for Scientific Research | CNRS · Institut de Biologie Moléculaire des Plantes (IBMP)

PhD DR CNRS, lab IBMP Strasbourg- team Chabouté

About

71
Publications
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1,760
Citations
Citations since 2016
27 Research Items
605 Citations
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
Introduction
Marie-Edith Chabouté currently works at the Institut de Biologie Moléculaire des Plantes (IBMP) , French National Centre for Scientific Research. Marie-Edith does research in Molecular Biology, Genetics and Cell Biology. Her lab is interested in plant nuclear mechanics and stress signaling.

Publications

Publications (71)
Article
Full-text available
Significance Centromeres are crucial as they avoid genomic instability during mitosis, but the mechanisms involved in their assembly and maintenance are not yet fully elucidated in eukaryotes. Here, we describe a previously unidentified aspect of centromere regulation mediated by γ-tubulin complex protein 3-interacting proteins (GIPs). Our data cor...
Article
Centromeres are chromatin structures required for the proper segregation of chromosomes during mitosis and meiosis. The centromere is composed of centromeric DNA, often enriched in satellite repeats, and kinetochore complex proteins. To date, over 100 kinetochore components have been identified in different eukaryotes. Kinetochore assembly begins w...
Article
Full-text available
The spindle assembly checkpoint (SAC) is a refined surveillance mechanism which ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the spindle microtubules (MT). The SAC has been extensively studied in metazoans and yeast, but little is known about its role in plants.We identified proteins interacting w...
Article
Full-text available
The functional organization of the nuclear envelope (NE) is only just emerging in plants with the recent characterization of NE protein complexes and their molecular links to the actin cytoskeleton. The NE also plays a role in microtubule nucleation by recruiting γ-Tubulin Complexes (γ-TuCs) which contribute to the establishment of a robust mitotic...
Article
Full-text available
During interphase, the microtubular cytoskeleton of cycling plant cells is organized in both cortical and perinuclear arrays. Perinuclear microtubules (MTs) are nucleated from γ-Tubulin Complexes (γ-TuCs) located at the surface of the nucleus. The molecular mechanisms of γ-TuC association to the nuclear envelope (NE) are currently unknown. The γ-Tu...
Preprint
Full-text available
Chromatin is a dynamic platform within which gene expression is controlled by epigenetic modifications, notably targeting amino acid residues of histone H3. Among them is Lysine 27 of H3 (H3K27), which trimethylation by the Polycomb Repressive Complex 2 (PRC2) is instrumental in regulating spatio-temporal patterns of key developmental genes. H3K27...
Article
Full-text available
The maintenance of genetic information is important in eukaryotes notably through mechanisms occurring at the nuclear periphery where inner nuclear membrane proteins and nuclear pore-associated components are key factors regulating the DNA damage response (DDR). However, this aspect of DDR regulation is still poorly documented in plants. We address...
Article
Full-text available
Brassinosteroids (BRs) play key roles in diverse plant growth processes through a complex signaling pathway. Components orchestrating the BR signaling pathway include receptors such as kinases, transcription factors, protein kinases and phosphatases. The proper functioning of the receptor kinase BRI1 and the transcription factors BES1/BZR1 depends...
Article
Full-text available
Root hairs (RHs) are tubular extensions of root epidermal cells that favour nutrient uptake and microbe interactions. RH shows a fast apical growth, constituting a unique single cell model system to analyse cellular morphodynamics. In this context, live cell imaging using microfluidics recently developed to analyze root development is appealing, bu...
Article
Full-text available
Significance During development, growth deforms tissues and organs. This is notably the case during the formation of new flowers in plants, as the tissue folds during young floral bud emergence. Here, we provide further evidence that organogenesis compresses the cells at the boundary, separating the organ from the stem cell niche, and we show that...
Article
Full-text available
An ongoing challenge in functional epigenomics is to develop tools for precise manipulation of epigenetic marks. These tools would allow moving from correlation-based to causal-based findings, a necessary step to reach conclusions on mechanistic principles. In this review, we describe and discuss the advantages and limits of tools and technologies...
Article
Full-text available
Beyond its biochemical nature, the nucleus is also a physical object. There is accumulating evidence that its mechanics plays a key role in gene expression, cytoskeleton organization, and more generally in cell and developmental biology. Building on data mainly obtained from the animal literature, we show how nuclear mechanics may orchestrate devel...
Article
Full-text available
Beyond its biochemical nature, the nucleus is also a physical object. There is accumulating evidence that its mechanics plays a key role in gene expression, cytoskeleton organization, and more generally in cell and developmental biology. Building on data mainly obtained from the animal literature, we show how nuclear mechanics may orchestrate devel...
Article
In Arabidopsis, the F-box protein FBL17 (F BOX-LIKE17) was previously identified as an important cell-cycle regulatory protein. FBL17 is required for cell division during pollen development and for normal cell-cycle progression and endoreplication during the diploid sporophyte phase. FBL17 was reported to control the stability of the CDK (CYCLIN-DE...
Article
In animal single cells in culture, nuclear geometry and stiffness can be affected by mechanical cues, with important consequences for chromatin status and gene expression. This calls for additional investigation into the corresponding physiological relevance in a multicellular context and in different mechanical environments. Using the Arabidopsis...
Article
Full-text available
Key message Rice rss1 complementation assays show that wheat TdRL1 and RSS1 are true functional homologs. TdRL1 over-expression in Arabidopsis conferred salt stress tolerance and alleviated ROS accumulation. Abstract Plants have developed highly flexible adaptive responses to their ever-changing environment, which are often mediated by intrinsical...
Article
Full-text available
Reversible phosphorylation is an essential mechanism regulating signal transduction during development and environmental stress responses. An important number of dephosphorylation events in the cell are catalyzed by type one protein phosphatases (PP1), which catalytic activity is driven by the binding of regulatory proteins that control their subst...
Data
cDNA sequences of Brachypodium and wheat PP1 genes. identified downloaded from phytozome and EnsemblPant. (DOCX)
Data
Most relevant cis-elements found in promoters of representative wheat genes identified using PlantPAN2.0. (DOCX)
Data
Phylogenetic analysis based on wheat, Arabidopsis, rice, Barachypodium cDNAs. Phylogenetic tree was obtained using MEGA6 with the Neighbor-Joining method based on cDNA sequences from EnsemblPlant, phytozome and TAIR. Wheat sequences are indicated with red dots, rice with light blue squares, Brachypodium PP1 genes with green diamonds and Arabidopsis...
Data
Phosphatase activity measurements. Recombinant His::PP1a phosphatase activity using OMFP as a substrate. Activities registered on 6xHis::TdPP1a protein in absence or in presence of Ca2+, Mg2+, Zn2+, Mn2+ and Fe2+ (1 mM each). Values are means of at least 3 independent experiments (± S.E). Stars represent statistical significance (Student’s T-test p...
Data
Relative expression of TdPP1. Seven-day-old durum wheat seedlings were treated with NaCl (150 mM), or ABA (100 μM) for 1 hour. (A) Relative expression of TdPP1a was analyzed by quantitative real-time qPCR using wheat actin as control. (B) Raw data of relative expression (RE) with standard error (SE). (TIF)
Data
PP1 wheat genes and their orthologs of T.urartu and A. tauschii. (DOCX)
Data
Wheat, Brachypodium and rice PP1 proteins. Accession number, size, molecular weight, pI, instability index and GRAVY. The latter prarameters were predicted with protparam. (DOCX)
Data
Evolutionary pairwise distances between wheat, Brachypodium, and rice PP1s by calculation of the Ka/Ks ratios. Ka/Ks ratios were calculated using MEGA6.06. (DOCX)
Article
Full-text available
In animals, it is now well established that forces applied at the cell surface are propagated through the cytoskeleton to the nucleus, leading to deformations of the nuclear structure and, potentially, to modification of gene expression. Consistently, altered nuclear mechanics has been related to many genetic disorders, such as muscular dystrophy,...
Article
Full-text available
The control of genomic maintenance during S phase is crucial in eukaryotes. It involves the establishment of sister chromatid cohesion, ensuring faithful chromosome segregation, as well as proper DNA replication and repair to preserve genetic information. In animals, nuclear periphery proteins - including inner nuclear membrane proteins and nuclear...
Chapter
In eukaryotes, both microtubules (MTs) and microfilaments (MFs) are cytoskeletal polymers involved in meristematic cell proliferation. While animal cells build their MT arrays from structured organelles, such as centrosomes, and while they depolymerise their MFs and become round during mitosis, vascular plant cells lack centrosomes, maintain a fila...
Article
Full-text available
Centromeres are known as specific chromatin domains without which eukaryotic cells cannot divide properly during mitosis. Despite the considerable efforts to understand the centromere/kinetochore assembly during mitosis, until recently, comparatively few studies have dealt with the regulation of centromere during interphase. Here, we briefly review...
Article
Centromeres play a pivotal role in maintaining genome integrity by facilitating the recruitment of kinetochore and sister-chromatid cohesion proteins, both required for correct chromosome segregation. Centromeres are epigenetically specified by the presence of the histone H3 variant (CENH3). In this study, we investigate the role of the highly cons...
Data
Supporting Information for "Three BUB1 and BUBR1/MAD3-related spindle assembly checkpoint proteins are required for accurate mitosis in Arabidopsis" (DOI: 10.1111/nph.13073). Fig. S1 Comparison of BUBR1/MAD3 and BUB1-related proteins in plants, yeasts and vertebrates. Fig. S2 Comparison of plant BUB1-related kinases and human BUB1. Fig. S3 Interact...
Article
The microtubular cytoskeleton plays a major role in cellular organization and proliferation. The first step leading to the building of a microtubule (MT) corresponds to MT nucleation. Individual MTs then participate in the organization of more complex MT arrays. A strong body of evidence suggests that the underlying molecular mechanisms involve pro...
Article
Full-text available
Microtubules (MTs) are crucial for both the establishment of cellular polarity and the progression of all mitotic phases leading to karyokinesis and cytokinesis. MT organization and spindle formation rely on the activity of γ-tubulin and associated proteins throughout the cell cycle. To date, the molecular mechanisms modulating γ-tubulin complex lo...
Article
Cellular responses to DNA double-strand breaks (DSBs) are linked in mammals and yeasts to the phosphorylated histones H2AX (γH2AX) repair foci which are multiproteic nuclear complexes responsible for DSB sensing and signalling. However, neither the components of these foci nor their role are yet known in plants. In this paper, we describe the effec...
Article
Full-text available
In plants, different forms of programmed cell death (PCD) have been identified, but they only partially correspond to those described for animals, which is most probably due to structural differences between animal and plant cells. Here, the results show that in tobacco BY-2 cells, bleomycin (BLM), an inducer of double-strand breaks (DSBs), trigger...
Article
*The Arabidopsis genome possesses two confirmed Cytochrome P450 Reductase (CPR) genes, ATR1 and ATR2, together with a third putative homologue, ATR3, which annotation is questionable. *Phylogenetic analysis classified ATR3 as a CPR-like protein sharing homologies with the animal cytosolic dual flavin reductases, NR1 and Fre-1, distinct from the mic...
Article
Full-text available
Ribonucleotide reductase (RNR) is an essential enzyme that provides dNTPs for DNA replication and repair. Arabidopsis (Arabidopsis thaliana) encodes three AtRNR2-like catalytic subunit genes (AtTSO2, AtRNR2A, and AtRNR2B). However, it is currently unclear what role, if any, each gene contributes to the DNA damage response, and in particular how eac...
Article
Re-activation of cell division after fertilization involves the specific regulation of a set of genes. To identify genes involved in the gametophytic to sporophytic transition, we screened Arabidopsis T-DNA insertion lines for early seed abortion at the zygote (zeus) or one-cell embryo stages (cyclops), and characterized a sporophytic zygote-lethal...
Article
Full-text available
Re-activation of cell division after fertilization involves the specific regulation of a set of genes. To identify genes involved in the gametophytic to sporophytic transition, we screened Arabidopsis T-DNA insertion lines for early seed abortion at the zygote (zeus) or one-cell embryo stages (cyclops), and characterized a sporophytic zygote-lethal...
Article
We compare cadmium and copper induced oxidative stress in tomato leaves and the antioxidative enzyme response during a time course of 96h. Plants were subjected to 25μM of CdCl2 or CuSO4 and malondialdehyde (MDA) level and activity of guaiacol peroxidase, superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase were determined...
Chapter
Heavy metal contamination in soils is easily transmitted to human through plants via the food chain. A major concern is to understand the plant response to heavy metal soil contamination to develop phytoremediation. Two plant models have been investigated in our study, the tomato, which is of agronomical importance, Arabidopsis, which is used as a...
Chapter
Full-text available
During the past ten years, emerging data revealed the role of E2F factors in various plant physiology aspects. E2F involvement in cell cycle and plant development was particularly investigated and highlighted some plant specificities, espacially in postembryonic development. A major role of E2F is its transcriptional activity governing specific gen...
Book
During the past ten years, emerging data revealed the role of E2F factors in various plant physiology aspects. E2F involvement in cell cycle and plant development was particularly investigated and highlighted some plant specificities, espacially in postembryonic development. A major role of E2F is its transcriptional activity governing specific gen...
Article
During Arabidopsis embryogenesis, the control of division between daughter cells is critical for pattern formation. Two embryo-defective (emb) mutant lines named quatre-quart (qqt) were characterized by forward and reverse genetics. The terminal arrest of qqt1 and qqt2 embryos was at the octant stage, just prior to the round of periclinal divisions...
Article
The promoters of several E2F-regulated genes identified in plants contain a variety of E2F motifs, notably a composite element consisting of a "CDE-like element" C/GGCGG on one strand, described as repressor in animals, associated with an E2F element on the complementary strand. This detailed study throughout plant development using ribonucleotide...
Article
Accurate DNA replication is one of the most important events in the life of a cell. To perform this task, the cell utilizes several DNA polymerase complexes. We investigated the role of DNA polymerase epsilon during gametophyte and seed development using forward and reverse genetic approaches. In Arabidopsis, the catalytic subunit of this complex i...
Article
Full-text available
E2F factors are implicated in various cellular processes including specific gene induction at the G1/S transition of the cell cycle. We present in this study a novel regulatory aspect for the tobacco large subunit of ribonucleotide reductase (R1a) and its encoding gene (RNR1a) in the UV‐C response. By structural analyses, two E2F sites were identif...
Article
Full-text available
The RB/E2F pathway is involved in the control of the G1/S transition of the eukaryotic cell cycle where various S phase genes are activated by specific E2F factors. Ribonucleotide reductase (RNR) plays an essential role in the DNA synthesis pathway. Earlier studies showed that there are at least twoRNR1 genes (RNR1a and RNR1b) and one RNR2 gene in...
Article
Full-text available
Ribonucleotide reductase (RNR) is a key enzyme involved in the DNA synthesis pathway. The RNR-encoded genes are cell cycle regulated and specifically expressed in S phase. The promoter of the RNR2 gene encoding for the small subunit was isolated from tobacco. Both in vivo and in vitro studies of the DNA-protein interactions in synchronized BY2 toba...
Article
Full-text available
Ribonucleotide reductase (RNR) is a key enzyme involved in the DNA synthesis pathway. The RNR-encoded genes are cell cycle regulated and specifically expressed in S phase. The promoter of the RNR2 gene encoding for the small sub-unit was isolated from tobacco. Both in vivo and in vitro studies of the DNA–protein interactions in synchronized BY2 tob...
Article
Although the basic mechanisms which control the progression through the cell cycle appear to be conserved in all higher eukaryotes, the unique features of the plant developmental programme must be somehow reflected in a plant-specific regulation of the factors which control cell division. In the last few years, considerable progress has been achiev...
Article
Eukaryotic ribonucleotide reductase (RNR), the enzyme involved in the synthesis of the deoxyribonucleotides, consists of two R1 and R2 subunits whose activities and gene expression are differentially regulated during the cell cycle and are preferentially induced at the G1/S transition. We have isolated three cDNA clones from a tobacco S phase libra...
Article
A new Arabidopsis thaliana (ecotype Columbia) genomic library has been constructed in Yeast Artificial Chromosomes: the CIC library (for CEPH, INRA and CNRS). Optimization of plant culture conditions and protoplast preparation allowed the recovery of large amounts of viable protoplasts. Mechanical shearing of DNA was minimized by isolation of DNA f...
Article
The primary structure of the plant histone genes has been deduced from the comparison of the nucleotide sequences of 23 genes and 14 cDNAs from eight different species. These data confirmed the extreme conservation of histones H3 and H4 in plant and animal kingdoms. Histone H2B is more variable than H2A and the histone H1 is the less conserved hist...
Article
The histone H3 and H4 genes are shown to be expressed in both Arabidopsis plantlets and transitory multicellular suspension. The 5′- and 3′-ends of the H4 mRNAs have been localized on two H4 genes previously sequenced, H4A748 and H4A777. S1-nuclease mapping and reverse-transcriptase-primer-elongation experiments revealed the existence of two start...
Article
Full-text available
Northern blot analysis revealed that the histone H3 and H4 mRNAs are of unusual large size in germinating maize embryos. S1-mapping experiments show that the 3′-untranslated regions of the mRNAs transcribed from 3 H3 and 2 H4 maize genes previously described are much longer than in the non-polyadenylated histone mRNAs which represent a major class...
Article
Two histone H3 and two histone H4 genes have been cloned from a λgtWESλ·B Arabidopsis thaliana gene library. From their nucleotide sequences and from studies on their genomic organization, the following conclusions can be drawn:1) The nucleotide sequences of the two H3 coding regions show only 85% homology, but encode the same proteins. The Arabido...
Article
The general structure of the plant histone genes has been deduced from the comparison of the nucleotide sequences of ten H3 and H4 genes of maize (3 H3 and 3 H4) and Arabidopsis thaliana (2 H3 and 2 H4). The five H3 and five H4 genes encode the same proteins, respectively. The 5′-flanking regions contain the classical histone gene-specific consensu...
Article
The sea urchin histone H4 gene has been used as a probe to clone two corn histone H4 genes from a lambda gtWES X lambda B corn genomic library. The nucleotide (nt) sequences of both genes showed that the encoded amino acid sequences were identical to that of the H4 of pea and one variant of wheat. The nt sequences of the coding regions showed 92% h...
Article
The sea urchin histone H4 gene has been used as a probe to clone two corn histone H4 genes from a λgl WES · λB corn genomic library. The nucleotide (nt) sequences of both genes showed that the encoded amino acid sequences were identical to that of the H4 of pea and one variant of wheat. The nt sequences of the coding regions showed 92% homology. 5'...

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Projects (4)
Project
Using a systems biology approach in the context of an interdisciplinary network, we will investigate how mechanical cues affect cellular shaping and are sensed at the nuclear envelope to drive chromatin remodelling in Arabidopsis. The aim of the international team is to combine micromechanical measurements, real-time fluorescence microscopy, biochemical analyses and numerical simulations to give rise to the first integrated model of root hair growth under the combined effect of genetics and constraints generated during growth. There is an open post-doc position in the lab.