Marie-Caroline Michalski

Marie-Caroline Michalski
French National Institute for Agriculture, Food, and Environment (INRAE) | INRAE · Cardiovasculaire, Métabolisme, diabétologie et Nutrition (CarMeN)

PhD, Food Engineer

About

205
Publications
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Introduction
Marie-Caroline Michalski currently works at the Cardiovasculaire, Métabolisme, Diabétologie et Nutrition laboratory (CarMeN), French National Institute for Agricultural Research (INRA). Marie-Caroline does research in Nutrition and Dietetics, Metabolism, Food Science and Nutritional Biochemistry. Her current projects are about 'Metabolic impact of dietary fat structure' (including intestinal absorption, postprandial lipemia, lipid metabolism and endotoxemia/inflammation).

Publications

Publications (205)
Article
Full-text available
Elevated concentrations of triglyceride-rich lipoproteins (TGRL) in the fasting and postprandial states are risk factors for cardiovascular events, especially in type 2 diabetes (T2D). T2D modifies the lipid composition of plasma and lipoproteins and some sphingolipids (SP) have been validated as potent predictive biomarkers of cardiovascular disea...
Article
Objectives Elevated concentrations of triglyceride-rich lipoproteins (TGRL) in the fasting and postprandial states are a risk factor for cardiovascular events in type 2 diabetes (T2D). Dyslipidemia in T2D is associated with modifications in the lipidome of plasma lipoproteins and some plasma sphingolipids (SP) have been validated as potent predicti...
Article
The specific activities of gastric and pancreatic lipases were measured using triacylglycerols (TAG) from rapeseed oil, purified 1,3-sn-DAG and 1,2(2,3)-sn-DAG produced from this oil, as well as a rapeseed oil enriched with 40% w/w DAG (DAGOIL). Gastric lipase was more active on 1,3-sn-DAG than on 1,2(2,3)-sn-DAG and TAG, whereas pancreatic lipase...
Article
Full-text available
This study evaluates the capacity of a bread enriched with fermentable dietary fibres to modulate the metabolism and nutrients handling between tissues, gut and peripheral, in a context of overfeeding. Net fluxes of glucose, lactate, urea, short chain fatty acids (SCFA), and amino acids were recorded in control and overfed female mini-pigs suppleme...
Article
Résumé Emulsifiants et stabilisants largement utilisés par l’industrie agro-alimentaire, les lécithines sont des mélanges complexes de lipides composés à plus de 50 % de phospholipides (PL), issus de sources animales ou végétales. Face à la nécessité de réduire les apports en produits d’origine animale et de limiter l’utilisation d’additifs de synt...
Article
Objectives Dietary synthetic emulsifiers have recently been shown to promote metabolic syndrome and alter gut microbiota. The effects of natural emulsifiers, such as vegetable lecithin, remain, however, poorly described. Our objective was to evaluate, in mice, the impact of soy and rapeseed lecithin, both rich in essential α-linolenic acid (ALA), w...
Article
Full-text available
In vitro digestion studies often use animal digestive enzyme extracts as substitutes of human gastric and pancreatic secretions. Pancreatin from porcine origin is thus commonly used to provide relevant pancreatic enzymes such as proteases, amylase and lipase. Rabbit gastric extracts (RGE) have been recently introduced to provide gastric lipase in a...
Article
Full-text available
BACKGROUND High circulating levels of ceramides (Cer) and sphingomyelins (SM) are associated with cardiometabolic diseases. The consumption of whole fat dairy products, naturally containing such polar lipids (PL), is associated with health benefits, but the impact on sphingolipidome remains unknown.METHODS In a 4-week randomized controlled trial, 5...
Article
Full-text available
Scope Synthetic emulsifiers have recently been shown to promote metabolic syndrome and considerably alter gut microbiota. Yet, data is lacking regarding the effects of natural emulsifiers, such as plant lecithins rich in essential α‐linolenic acid (ALA), on gut and metabolic health. Methods and Results For 5 days, male Swiss mice were fed diets co...
Article
Full-text available
The metabolic impact of dietary lipids needs to be considered beyond the fatty acid profile and energetic value of such lipids. Fatty acids are the building blocks of the different lipid molecules, including triacylglycerols and phospholipids, which are organized within various supramolecular structures such as emulsion droplets. These structures c...
Chapter
Milk presents a unique lipid variety, including several hundreds of different fatty acid species with various bioactivities. However, bovine milk fat still suffers from a bad image in the lay public because it is rich in saturated fatty acids, which are perceived as associated with deleterious health effects only. In contrast, recent meta-analyses...
Article
Background: Soybean lecithin, a plant-based emulsifier widely used in food, is capable of modulating postprandial lipid metabolism. With arising concerns of sustainability, alternative sources of vegetal lecithin are urgently needed, and their metabolic effects must be characterized. Objectives: We evaluated the impact of increasing doses of rap...
Article
Purpose of review: The impact of dietary lipids on cardiometabolic health was mainly studied considering their fatty acid composition. This review aims to present the recent change in paradigm whereby the food matrix, the molecular and supramolecular structures of dietary lipids modulate their digestive fate and cardiometabolic impact. Recent fin...
Article
Full-text available
Scope. The aim of this study was to examine whether postprandial (PP) triglyceride‐rich lipoproteins (TGRL) secreted after a moderate fat intake would activate platelets differently according to their fatty acid (FA) composition. Methods and results. In a parallel single‐blind randomized trial, 30 women with type 2 diabetes were assigned to a break...
Article
Full-text available
Circulating levels of lipopolysaccharide-binding protein (LBP) and soluble cluster of differentiation 14 (sCD14) are recognized as clinical markers of endotoxemia. In obese men, postprandial endotoxemia is modulated by the amount of fat ingested, being higher compared to normal-weight (NW) subjects. Relative variations of LBP/sCD14 ratio in respons...
Article
Full-text available
Oxylipins and endocannabinoids (eCBs) both belong to superclasses of lipid mediators with potent inflammation modulatory activities. The adipose tissue (AT) plays a key role in metabolic syndrome-related inflammation, via altered adipocyte physiology, infiltrated macrophages and altered profile of eCBs. We previously reported that DHA is more poten...
Article
Objectives Dietary synthetic emulsifiers have recently been shown to promote metabolic syndrome and considerably alter gut microbiota. Conversely, natural emulsifiers such as milk polar lipids (PL) are associated with beneficial metabolic effects. The effects of plant PL remain, however, poorly described. Our objective was to evaluate, using two co...
Chapter
Dairy products are basic products largely consumed in the population, from human milk which is the perfect meal for the newborn to a large variety of dairy products from cow and other mammalians. Dairy products consumption has been recommended for its richness in valuable nutrients, but some research some 30 years ago raised concern on dairy lipid...
Article
Donor human milk, pasteurised for safety reasons, is the first alternative for feeding preterm infants when mothers' own milk is unavailable. Breastmilk pasteurization impact on lipid digestion and absorption was evaluated by a static in vitro digestion model for preterm infants coupled with intestinal absorption using Caco-2/TC7 cell. Lipid absorp...
Article
Chronic undernutrition leads to growth hormone resistance and poor growth in children, which has been shown to be modulated by microbiota. We studied whether Lactobacillus fermentum CECT5716 (Lf CECT5716), isolated from mother’s breast milk, could promote juvenile growth through the modulation of lipid absorption in a model of starvation. Germ-free...
Chapter
More than 90% of the lipids are present in the diet as triglycerides (TG), carrying three esterified dietary fatty acids (FAs) per triglyceride molecule. Phospholipids (PLs) are other important carriers of dietary FAs. Altogether, the daily intake of dietary FAs from TG and PLs will not ultimately lead to the same amount of FAs being released in th...
Article
Vegetable lecithins, widely used in the food industry as emulsifiers, are a mixture of naturally occurring lipids containing more than 50% of phospholipids (PL). PL exert numerous important physiological effects. Their amphiphilic nature notably enables them to stabilise endogenous lipid droplets, conferring them an important role in lipoprotein tr...
Article
The increasing prevalence of obesity and metabolic diseases is a worldwide public health concern, and the advent of new analytical technologies has made it possible to highlight the involvement of some molecules, such as sphingolipids (SL), in their pathophysiology. SL are constituents of cell membranes, lipoproteins and lipid droplets (LD), and ar...
Article
Dietary fats are present in the diet under different types of structures, such as spread vs emulsions (notably in processed foods and enteral formula), and interest is growing regarding their digestion and intestinal absorption. In clinical trials, there is often a need to add stable isotope-labeled triacylglycerols (TAGs) as tracers to the ingeste...
Article
The identification of natural bioactive compounds aimed at promoting optimal gut health and improving lipid metabolism is paramount in the prevention of chronic disease. In this review, we summarize basic science and clinical research examining the protective properties of milk sphingomyelin (SM) against dysfunctional lipid metabolism, gut dysbiosi...
Article
Full-text available
Objective To investigate whether milk polar lipids (PL) impact human intestinal lipid absorption, metabolism, microbiota and associated markers of cardiometabolic health. Design A double-blind, randomised controlled 4-week study involving 58 postmenopausal women was used to assess the chronic effects of milk PL consumption (0, 3 or 5 g-PL/day) on...
Poster
Full-text available
Objectives: Nutritional strategies can play a major role in the management of cholesterolemia, notably in postmenopausal women at risk of CVD. Interest has recently grown on the potential health benefits of milk polar lipids (MPL). We showed that isolipidic enrichment of the diet with MPL improved several lipid CV risk factors but underlying mecha...
Article
Aims: Type 2 diabetes (T2D) patients present with risk factors for atherothrombosis such as fasting hypertriglyceridaemia and platelet hyperactivity. Our study objective was to determine the effect of large triglyceride-rich lipoproteins (TGRL) from fasting T2D patients on platelet aggregation and, if any, to identify the signaling pathway involve...
Article
Scope : Obese adipose tissue (AT) is infiltrated by inflammatory immune cells including IL‐17A‐producing‐T (Th17) cells. We have previously demonstrated that adipose‐derived stem cells from obese (ob‐ASC), but not lean AT promote Th17 cells. Because n‐3 PUFA are known to inhibit obese AT inflammation, here we tested whether they could inhibit ob‐AS...
Article
Discipline Clinique. Introduction et but de l’étude Des perturbations du métabolisme lipidique à jeun et en phase postprandiale constituent des facteurs de risque cardiovasculaire. La nutrition joue un rôle majeur dans leur modulation, notamment chez les femmes ménopausées qui constituent une population à risque. Un intérêt s’est récemment dévelop...
Article
Discipline Éxpérimental/mécanismes cellulaires et moléculaires. Introduction et but de l’étude L’obésité est associée à des altérations du microbiote et de la barrière intestinale et à l’établissement d’une inflammation à bas bruit. Dans ce contexte, les lipides polaires laitiers (LP) ont montré des effets bénéfiques sur le métabolisme lipidique c...
Article
Mol. Nutr. Food Res. 2019, 63, 1801078 DOI: 10.1002/mnfr.201801078 Polar lipids of the milk fat globule membrane are an emerging functional ingredient. In article number 1801078, Marie‐Caroline Michalski and co‐workers show in mice that when added to a high‐fat diet, 1.6% of milk polar lipids reduces body weight gain, modulates the abundance of bac...
Article
Scope : Enhanced adiposity and metabolic inflammation are major features of obesity associated with altered gut microbiota and intestinal barrier. We investigated in mice fed a mixed high‐fat (HF) diet how these metabolic outcomes could be impacted by milk polar lipids (MPL), naturally containing 25% of sphingomyelin. Methods and results : Male C5...
Article
Milk polar lipids (MPL) are specifically rich in milk sphingomyelin (MSM) which represents 24% of MPL. Beneficial effects of MPL or MSM have been reported on lipid metabolism, but information on gut physiology is scarce. Here we assessed whether MPL and MSM can impact tight junction expression. Human epithelial intestinal Caco-2/TC7 cells were incu...
Article
Although UHT heat treatment is being optimized to improve the stability and functional properties of dairy products, its metabolic effects remain scarcely known. As such, we studied the effect of the type of UHT process on lipid metabolism, intestinal barrier, and inflammation in mice. Nine-week-old male C57Bl/6J mice were fed a diet composed of no...
Article
Introduction et but de l’étude L’hyperlipémie postprandiale et l’altération de la bêta-oxydation des lipides sont des facteurs de risque métaboliques liés à l’obésité et en lien direct avec l’apport alimentaire en lipides. La quantité et la taille des chylomicrons sont connus pour conditionner leur élimination et sont capables de moduler la réparti...
Article
Introduction et but de l’étude La dénutrition représente un surcoût élevé hospitalier même dans les pays industrialisés. La dénutrition a un impact important sur la croissance infantile. La présence d’un microbiote intestinal est indispensable au maintien d’une croissance juvénile. En situation de dénutrition chez l’animal, certaines souches de lac...
Article
Introduction et but de l’étude Les régimes occidentaux sont impliqués dans le développement des maladies métaboliques, dont la composante inflammatoire peut être due à une altération de la barrière intestinale. Les produits laitiers contiennent naturellement la matière grasse sous forme de globule gras, constitués (i) d’un cœur de triglycérides (98...
Article
Additives stabilize or improve the organoleptic or functional properties (or both) of many dairy products including whipping cream. Their influence on the metabolic effect of dairy cream is scarcely known. We tested the hypothesis that added emulsifier (lactic acid esters of mono- and diglycerides; MAG/DAG), thickener (carrageenan, CGN), or both, c...
Article
Although UHT heat treatment is being optimized to improve the stability and functional properties of dairy products, its metabolic effects remain scarcely known. As such, we studied the effect of the type of UHT process on lipid metabolism, intestinal barrier, and inflammation in mice. Nine-week-old male C57Bl/6J mice were fed a diet composed of no...
Article
Full-text available
Abetalipoproteinemia (ABL) and chylomicron retention disease (CMRD) are extremely rare recessive forms of hypobetalipoproteinemia characterized by intestinal lipid malabsorption and severe vitamin E deficiency. Vitamin E is often supplemented in the form of fat-soluble vitamin E acetate, but fat malabsorption considerably limits correction of the d...
Article
Full-text available
Obesity and its metabolic complications are characterized by subclinical systemic and tissue inflammation. In rodent models of obesity, inflammation and metabolic impairments are linked with intestinal barrier damage. However, whether intestinal permeability is altered in human obesity remains to be investigated. In a cohort of 122 severely obese a...
Article
Full-text available
Background Better choices of dietary lipid sources and substitution of refined by fortified oils could reduce the intake of saturated fatty acids (FA) and increase the intake of omega 3 FA concomitantly to healthy bioactive compounds. Methods The development of obesity and metabolic disturbances was explored in rats fed during 11 weeks with a high...
Article
Full-text available
Background Obesity progressively leads to cardiac failure. Omega-3 polyunsaturated fatty acids (PUFA) have been shown to have cardio-protective effects in numerous pathological situations. It is not known whether rapeseed oil, which contains α-linolenic acid (ALA), has a similar protective effect. Omega-3 PUFAs are sensitive to attack by reactive o...
Book
Le fromage est un produit connu et élaboré par l’homme depuis des millénaires. Il est lié à la domestication des espèces laitières et à la connaissance empirique de la richesse nutritionnelle du lait, la transformation du lait en fromage répondant au besoin de conservation de cet aliment.Le Fromage réunit l’essentiel des connaissances aujourd’hui d...
Chapter
Beyond fatty acid composition of milk fat that can impact the health of consumers, interest is growing regarding the importance of the supramolecular structure of milk lipids on their nutritional properties. Indeed, milk fat is found naturally under the form of an emulsion of lipid droplets called the milk fat globule (MFG) composed of a triglyceri...
Article
Bioactive lipids of the milk fat globule membrane become concentrated in two co-products of the butter industry, buttermilk and butterserum. Their lipid composition is detailed here with special emphasis on sphingolipid composition of nutritional interest, determined using GC, HPLC and tandem mass spectrometry. Butterserum was 2.5 times more concen...
Article
Full-text available
Background Postprandial hyperlipemia is recognized as a major cardio-metabolic risk factor, recently linked to the co-absorption of pro-inflammatory lipopolysaccharides with dietary lipids. This causes endotoxemia that is involved in the pathophysiology of obesity and insulin resistance, but to date the impact of food formulation is unknown. We tes...
Article
Résumé Les effets des lipides sur la santé doivent aujourd’hui être étudiés par des approches allant au-delà de leur densité énergétique et de leur profil en acides gras. En effet, ces acides gras sont les briques élémentaires de différentes molécules lipidiques telles que les triacylglycérols et les phospholipides, elles-mêmes organisées sous form...
Article
Full-text available
Foods consist of a large number of different nutrients that are contained in a complex structure. The nature of the food structure and the nutrients therein (i.e., the food matrix) will determine the nutrient digestion and absorption, thereby altering the overall nutritional properties of the food. Thus, the food matrix may exhibit a different rela...
Article
Full-text available
Les effets des lipides sur la santé doivent aujourd'hui être étudiés par des approches allant au-delà de leur densité énergétique et de leur profil en acides gras. En effet, ces acides gras sont les briques élémentaires de différentes molécules lipidiques telles que les triacylglycérols et les phospholipides, elles-mêmes organisées sous forme de st...