Mariangela Albertini

Mariangela Albertini
University of Milan | UNIMI · Department of Veterinary Medicine (DiMeVet)

About

88
Publications
11,116
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,199
Citations
Additional affiliations
April 2016 - May 2016
University of Milan
Position
  • Professor (Associate)
January 2010 - March 2016
University of Milan
Position
  • false
January 2000 - March 2016
University of Milan
Position
  • Professor (Associate)

Publications

Publications (88)
Article
The need for faster diagnosis and more accurate treatment decisions in separation-related problems (SRPs) in dogs is urgent, and a more precise behavioral phenotyping and the development of biomarkers may be of great value. Vasopressin could be a potential non-invasive biomarker of anxiety in dogs with SRPs, but reliable measurement of its concentr...
Article
Full-text available
Quality of life is defined as an individual’s satisfaction with its physical and psychological health, its physical and social environment, and its ability to interact with the environment. Understanding companion dogs’ QOL can help veterinarians and owners know when treatment options have successfully alleviated symptoms of disease in such fields...
Article
Full-text available
Behavioural reactions towards a dead conspecific have been observed rarely in wild canids and there is no documented scientific evidence of grief in pet dogs. A quantitative analysis of grief-related responses in both dogs and owners was conducted, using the validated online Mourning Dog Questionnaire. The survey was completed by 426 Italian adults...
Article
Full-text available
Dogs are biologically predisposed to communicate with humans in cooperative contexts. They show individual differences in dog–human communication and inhibition, potentially enhanced by life experience, e.g., Animal-Assisted Interventions. This study aimed to investigate whether dogs’ personality, defined by biologically meaningful neural circuits...
Article
Full-text available
The COVID-19 pandemic caused lifestyle changes, with unknown effect on pets’ quality of life (QoL). Between May and July 2020, we distributed an online survey to investigate the role of several factors on feline and canine QoL, including lockdown-related factors. We used existing scales to measure human and pets’ personalities (Reinforcement Sensit...
Article
Early detection of lung cancer (LC) is a priority since LC is characterized by symptoms mimicking other respiratory conditions, but remains the leading cause of oncological disease death. Properly trained dogs can perceive the volatile organic compounds (VOCs) related to cancer, thanks to their acute sense of smell. Dogs' use for LC detection could...
Article
Full-text available
Physiological biomarkers of canine anxiety have not been extensively investigated to date. To identify new biomarkers in dogs, we compared behaviorally normal dogs (Control group, N = 13) to dogs diagnosed with separation problems (Case group, N = 13) as they were introduced into a novel environment in the presence of two strangers and subjected to...
Article
Full-text available
People often develop strong emotional connections with their dogs and consider them to be members of the family. The purpose of this study was to develop a novel validated tool, the Mourning Dog Questionnaire, to recognise and evaluate the mourning process in people who have lost a dog. The research model was based on a grid of five different quest...
Article
Full-text available
Poor knowledge is available on the effectiveness of reading to dogs in educational settings, particularly in children with Autism Spectrum Disorders (ASD). In this study, we test the hypothesis that reading to a dog improves propensity towards books and motivation to read after the end of the programme, as well as reading and cognitive skills in ch...
Article
Full-text available
As members of the public and the veterinary profession are increasingly concerned about animal welfare, there has been an increased scholarly interest in the attitudes of veterinarians and students toward animals, as these may impact human behavior, which ultimately impacts animal welfare. Here we investigated Italian veterinary students’ demograph...
Article
Previous studies suggest an improvement in both reading skills and attitudes towards reading when children read in the presence of a dog.This seems to be related to dogs being fully capable of acting as active and supportive listeners. However, little is known about the potential welfare implications in dogs involved in these activities. Although d...
Article
Full-text available
We investigated the attitudes of veterinary students towards animals and their welfare in Italy. Regression analyses revealed predictors that are significant in differentiating students' scoring tendency based on their gender, familiarity, and intention to work with a specific animal species, type of diet, and membership in an animal rights associa...
Article
Full-text available
Many studies have analyzed the behavior of puppies during their socialization period, while little attention has been paid to the transitional period, when vision and hearing develop. Here, we compared the average age of sensory and motor development, and the behavior among a total of 25 puppies. Each litter was videotaped during 1-hour daily sessi...
Article
Synchrony – the coordination of behavior between interacting partners – is a complex phenomenon requiring the perception and integration of multimodal communicative signals. Originally conceptualized by developmental psychologists to study the human-human relationship, it could also apply to cross-species interactions. Here we examined synchrony pa...
Article
Full-text available
Three brown bear (Ursus arctos arctos) individuals and two sloth bear (Melursus ursinus inornatus) individuals were observed in captivity to produce behavioural profiles for each individual. Data collected through behavioural observations were used to produce activity budgets, and to identify space usage and certain aspects of social behavior. Beha...
Article
Early diagnosis of cancer using effective screening methods is crucial for successful treatment. Recently, much attention has been given to the use of odors emitted in the form of volatile organic compounds (VOCs) as diagnostic biomarkers. Studies on special training of dogs to detect different cancers using various odor samples (breath, urine, can...
Chapter
These proceedings contain oral and poster presentations from various experts on animal behaviour and animal welfare in veterinary medicine presented at the conference.
Article
Full-text available
Animal personality is a growing research area due to the increasing evidence of the impact that it has on welfare, health and management of animals in captivity (Freeman and Gosling, 2010). Therefore, understanding, testing and improving existent methodologies, as well as develop new ones, to outline animal personality is an important step towards...
Article
Background: Extracorporeal treatments always require blood anticoagulation. We tested feasibility and efficacy of a novel technique for regional extracorporeal blood anticoagulation based on calcium removal by ion-exchange resins (i-ER), called ion-exchange resin anticoagulation (i-ERA). Methods: Eight swine were connected to a veno-venous extra...
Article
Several researches have evidenced that cancer cells can produce volatile organic compounds (VOCs) which are released not only in breath but also in other organic fluids, such as blood and urine. This study has evaluated the olfactory capability of trained dogs to detect human lung cancer VOCs in urine. We recruited 150 subjects from European Instit...
Article
There is longstanding recognition of the adverse effect of stressful experiences during early critical developmental periods and the later association with problematic behavioral issues in dogs. The aim of this study was to evaluate whether the origin/source of puppies (pet shop vs. breeder) was associated with later potential problematic behaviors...
Article
Full-text available
Interactions that animals experience can have a significant influence on their health and welfare. These interactions can occur between animals themselves, but also between animals and keepers, and animals and the public. Human and non-human animals come into contact with each other in a variety of settings, and wherever there is contact there is t...
Article
Full-text available
RATIONALE: We developed an innovative, minimally invasive, highly efficient, extracorporeal CO2 removal (ECCO2R) technique called Respiratory Electrodialysis (R-ED). OBJECTIVES: To evaluate the efficacy of R-ED in controlling ventilation compared to conventional ECCO2R-technology. METHODS: Five mechanically ventilated swine were connected to a cust...
Article
Unwelcome behaviors in pet dogs may have serious implications for the quality of life of both the animals and their owners. We investigated owners' perceptions about their dogs' behavioral issues as well as other factors that might be predictive of potential canine problematic behaviors. We distinguished between "undesirable behaviors" (behaviors t...
Article
Mosquito-borne pathogens pose major threats to both wildlife and human health and, largely as a result of unintentional human-aided dispersal of their vector species, their cumulative threat is on the rise. Anthropogenic climate change is expected to be an increasingly significant driver of mosquito dispersal and associated disease spread. The pote...
Article
The correlation between people characteristics and ownership of dog of a given size over others was investigated surveying a sample of Italian dog owners over the Internet. The regression analyses revealed predictors which were significant in differentiating owners of small dogs from the others. They were significantly more likely to have a low edu...
Article
Restenosis due to intimal hyperplasia is a major clinical problem that compromises the success of angioplasty and endovascular surgery. Resveratrol (RSV) has demonstrated a beneficial effect on restenosis from angioplasty. Unfortunately, the physicochemical characteristics of RSV reduce the practicality of its immediate clinical application. This w...
Article
Extracorporeal carbon dioxide removal has been proposed to achieve protective ventilation in patients at risk for ventilator-induced lung injury. In an acute study, the authors previously described an extracorporeal carbon dioxide removal technique enhanced by regional extracorporeal blood acidification. The current study evaluates efficacy and fea...
Article
Extracorporeal (EC) carbon dioxide (CO2) removal (ECCO2R) may be a powerful alternative to ventilation, possibly avoiding the need for mechanical ventilation and endotracheal intubation. We previously reported how an infusion of lactic acid before a membrane lung (ML) effectively enhances ECCO2R. We evaluated an innovative ECCO2R technique based on...
Article
Full-text available
Blood acidification by lactic acid infusion converts bicarbonate to CO2. This effect can be exploited to increase the transmembrane PCO2 gradient of an extracorporeal membrane lung, resulting in a significant increase of extracorporeal CO2 removal. Lactic acid, however, is an energetic substrate and its metabolism might increase total body CO2 prod...
Article
We recently demonstrated that injection of conditioned medium (CM) generated from cells of the mesenchymal region of human amniotic membrane (AMTCs) reduces bleomycin-induced lung fibrosis in mice, suggesting a crucial role of paracrine factor(s) secreted by AMTCs in these beneficial effects. We further investigated this hypothesis, the mechanisms...
Article
Objective: To evaluate plasma concentrations of inflammatory mediators in dogs with brachycephalic airway obstruction syndrome, identify a possible role for these mediators in the syndrome, and investigate the relationship between plasma concentrations of inflammatory mediators and severity of clinical signs. Animals: 17 dogs with brachycephalic...
Article
The present study examined the prevalence of behaviours in dogs separated from the litter for adoption at different ages. Seventy adult dogs separated from their dam and littermates and adopted between the ages of 30 and 40 days were compared with 70 adult dogs that had been taken from the litter for adoption at two months. Owners were asked to com...
Article
Full-text available
Agility, one of the last dog activities to gain formal recognition, tests both dog’s athleticism and dog–handler affinity. Although this activity seems to be enjoyed by dogs, it can be a stressful event. The level of stress in dogs can be measured by behavioral and physiological parameters. The purpose of this study was to evaluate the occurrence o...
Article
Full-text available
Mechanical ventilation is essential in intensive care units. However, it may itself induce lung injury. Current studies are based on rodents, using exceptionally large tidal volumes for very short periods, often after a "priming" pulmonary insult. Our study deepens a clinically relevant large animal model, closely resembling human physiology and th...
Article
Extracorporeal CO2 removal is an effective procedure to allow a protective ventilatory strategy in ARDS patients, but it is technically challenging due to the high blood flow required. Increasing the CO2 transfer through the membrane lung (ML) may lower the demand of extracorporeal blood flow and consequently allow for a wider clinical application...
Article
Full-text available
We studied the protection of recombinant human activated protein C (rhAPC) in endotoxin-induced lung inflammation and injury and whether this effect is correlated with modulation of lung matrix metalloproteinase (MMP) activity. We randomly assigned 12 Large White pigs to receive intravenous Escher-ichia coli lipopolysaccharide (LPS; 40 mu g/kg/hr),...
Article
Mazzola, S., Albertini, M., Pastore, C., Pirrone, F. and Clement, M.G., 2007. Carbon monoxide induced prevention of vascular ristenosis is not related to nitric oxide activity. Veterinary Research Communications, 31(Suppl. 1), 177–179
Article
Pirrone, F., Albertini, M., Mazzola, S., Pastore, C., Clement, M.G., Benfatto, M.C., Aldini, G. and Carini, M., 2007. Intravenous infusion of nitric oxide in experimental pulmonary hypertension: biotransformation and haemodynamics. Veterinary Research Communications, 31(Suppl. 1), 185–187
Article
The aim of this study was to investigate and quantify respiratory mechanical dysfunctions in Standardbred horses with both poor performance and bronchoalveolar lavage fluid cytology characteristic of inflammatory airway disease (IAD). A control group of healthy Standardbred horses was compared. Respiratory mechanics and breathing pattern were exami...
Article
Full-text available
The aim of this study was to elucidate the vasodilating mechanism of sodium nitroprusside (SNP). To do this, SNP was intravenously infused in pigs (1.67 micromol/kg), and the following paramagnetic metabolites were identified by electron spin resonance: 1) nitrosylhemoglobin [HbFe(II)NO] as an index of the bioconservative pathway; 2) transferrin; 3...
Article
Full-text available
INTRODUCTIONNitric oxide is a critical regulator of endothelial functions and vascular smooth muscletone. In a previous work, we demonstrated that under physiological conditions NO in-fusion markedly raises the formation of bioactive molecules, such as nitrosylhemoglobin[HbFe(II)NO], without evoking significant hemodynamic responses (Aldini
Article
Full-text available
Endotoxic shock, one of the most prominent causes of mortality in intensive care units, is characterized by pulmonary hypertension, systemic hypotension, heart failure, widespread endothelial activation/injury, and clotting culminating in disseminated intravascular coagulation and multi-organ system failure. In the last few years, studies in rodent...
Article
Endothelin (ET)-1 is a potent vasoconstrictive peptide and it is involved in the pathogenesis of septic shock. Blockade of ET-1 receptors abolishes the LPS-induced pulmonary hypertension and worsens the LPS-dependent systemic hypotension, but the role of ET-1 in sepsis remains uncertain. To determine the role of ET-1 in cardiovascular and respirato...
Article
Electromyographic examination (EMG) is a diagnostic technique, useful to discriminate between myogenic and neurogenic problems. In our study we used a multi-MUP EMG analysis, an online method that, permitting to know the results in real time, may be advantageous especially in uncooperative and not sedated animals. The EMG examination was performed...
Article
A saturated nitric oxide (NO) solution (1.88 mM) infused i.v. in the anesthetized pig at a dose of 68 nmol/kg/min for 24 min resulted in a time-dependent increase of nitrosylhemoglobin [HbFe(II)NO] as determined by electron spin resonance (ESR), reaching a C(max) of 7.99 +/- 0.42 microM at the end of the infusion, compared to 1.13 +/- 0.42 microM b...
Article
Full-text available
Horses display remarkable aerobic capabilities, attaining during muscular exercise a maximal rate of oxygen consumption about 30-fold higher than the resting value, and 2.5-fold higher than that of other mammals of similar body mass. Under these circumstances an enormous mechanical burden is expected to impinge on the equine respiratory pump and re...
Article
The role of endothelin ETA receptors in sepsis-induced mortality and edema formation was evaluated with a selective antagonist ABT-627 [2-(4-methoxyphenyl)-4-(1,3-benzodioxol-5-yl)-1-(N,N-di(n-butyl)amino carbonylmethyl)-pyrrolidine-3-carboxylic acid]. Sprague-Dawley rats received saline (control group), Escherichia coli endotoxin (10 mg/kg, sepsis...
Article
In a porcine model of endotoxic shock, we evaluated the circulatory and respiratory effects of NO synthase (NOS) blockade. Twenty anaesthetised pigs were divided into three groups and studied for 240 min after induction of endotoxic shock with lipopolysaccharides of Escherichia coli (LPS). After 180 min of endotoxic shock, one group (n = 6) receive...
Article
We evaluated the endothelin-1 (ET-1) and thrombin involvement in cardiovascular and respiratory dysfunction during endotoxic shock in 18 anaesthetized, mechanically ventilated pigs, divided into three groups. Group 1 was pre-treated only with lipopolysaccharide (LPS), group 2 was treated with lepirudin, a thrombin inhibitor, group 3 was pre-treated...
Article
To evaluate the role of endothelin (ET) in respiratory homeostasis we studied the effects of the ET(A) and ET(B) receptor blocking agent bosentan on respiratory mechanics and control in seven anaesthetised spontaneously breathing pigs, for 180 min after single bolus administration (20 mg/kg i.v.). The results show that the block of ET receptors ind...
Article
We evaluated the role of endothelin-1 (ET-1) and the involvement of nitric oxide in cardiovascular and respiratory dysfunction, during endotoxic shock, in 18 anaesthetised, mechanically ventilated pigs, divided into three groups. Group 1 was i.v. infused with LPS (20 microg/Kg/h for 240 min). Group 2 was pre-treated with bosentan, a dual inhibitor...
Article
To assess the contribution of poly (adenosine 5'-diphosphate ribose) synthetase (PARS) to the development of bacterial lipopolysaccharide (LPS)-induced acute lung injury and vascular failure in pigs. Four groups of anesthetized, paralyzed, and mechanically ventilated domestic white pigs. Group 1 served as control, whereas Escherichia coli LPS (20 m...
Article
In anaesthetised, paralysed and mechanically ventilated pigs we evaluated the involvement of ET-1 on PAF-dependent respiratory effects, blocking ET A and ETB receptors with BQ123 and BQ788 respectively. To avoid interference by nitric oxide we used L-NAME, an inhibitor of both NO synthases. The results show that PAF causes a decrease in Crs and an...
Article
A video-laryngoscopic method, implemented with an algorithm for the correction of the deformation inherent in the endoscope optical system, has been used to measure the dorsoventral diameter (Drg) and the cross-sectional area (CSArg) of the rima glottidis in five healthy workhorses during conscious breathing at rest. Simultaneous recording of the r...
Article
SUMMARYA video-laryngoscopic method, implemented with an algorithm for the correction of the deformation inherent in the endoscope optical system, has been used to measure the dorsoventral diameter (Drg) and the cross-sectional area (CSArg) of the rima glottidis in five healthy workhorses during conscious breathing at rest. Simultaneous recording o...
Article
This study investigated the mechanisms that may contribute to the hypoxic pulmonary vasoconstriction and compared the effects of hypoxia on pulmonary and systemic vascular beds. Six anesthetized spontaneously breathing pigs inhaled a hypoxic mixture (10% O2 in air) in control conditions and after pre-treatment with Indomethacin (3 mg kg(-1) i.v.) t...
Article
In 12 anaesthetized spontaneously breathing pigs divided into two groups of six animals we evaluated the respiratory and haemodynamic responses to endothelin-1 (ET-1) administered by aerosol (200 pmol x kg(-1) in 1 ml of saline solution). In the first group (control group), the responses to ET-1 were evaluated before and after the blocking of endog...
Article
The aim of the study was to evaluate the effects of nitric oxide (NO) on diaphragmatic fatigue in fifteen anaesthetized, mechanically ventilated pigs, divided into three groups. The animals were pre-treated with indomethacin (3 mg kg-1, i.v.) to block the cyclo-oxygenase pathway. To group 1 pigs (n = 6) NG-nitro-L-arginine methyl ester (L-NAME, 5 m...
Article
The study was performed in twelve anesthetized spontaneously breathing pigs, divided into two groups. In the animals of group 1 we evaluated the effects of ET-1 administered by aerosol (200 pmol/Kg in 1 ml of saline solution) on respiratory neural drive and on diaphragmatic activity. In the animals of group 2, to study the effects of ET-1 in the ab...
Article
The vascular effects of endothelin-1 (ET-1) and the release of prostacyclin and nitric oxide (NO) evoked by this peptide were analyzed in anesthetized, mechanically ventilated pigs. ET-1 induced biphasic responses in both the pulmonary and systemic vascular beds characterized by a transient hypotension followed by a long-lasting hypertension. To ev...
Article
In anesthetized, ventilated pigs we analyzed the involvement of nitric oxide (NO) and prostaglandins (PGs) in the regulation of systemic and pulmonary basal vascular tone. Endogenous release of NO was blocked by NG-nitro-L-arginine methyl ester (L-NAME) and prostanoid biosynthesis by indomethacin. Blocking NO raised pulmonary and systemic arterial...
Article
An in vivo porcine model of endotoxaemia was used to study the effects of glibenclamide, a K+ ATP-sensitive potassium channel blocker. Escherichia coli lipopolysaccharides (LPS, 70 micrograms/kg, i.v., as a bolus) were infused into anaesthetized, mechanically ventilated, indomethacin-treated pigs. After 120 min of endotoxaemia, glibenclamide was ad...
Article
We evaluated the involvement of K+ATP channels in the regulation of pulmonary and systemic vascular tone in anaesthetised, mechanically ventilated, indomethacin-treated pigs. The animals were divided into three groups of six pigs. To induce opening of K+ATP channels, to the first group we gave cromakalim as a bolus of 80 mu-m/ml, i.v. followed by d...
Article
We infused cromakalim, a K+ATP-sensitive channel opener (80μg/kg as a bolus, followed by 1μg/kg/min, drop by drop infusion) into 15 anaesthetised, mechanically ventilated, indomethacin-treated pigs. In six of these animals, 120 min after hypotension had been evoked by cromakalim, glibenclamide (10 mg/kg), a K+ATP-sensitive channel antagonist, was a...
Article
The respiratory and vascular effects of Endothelin-1 (ET-1) were evaluated in six anesthetized and spontaneously breathing pigs. ET-1 was administered intrajugularly as a bolus of 100 pmol/Kg. Then the vascular effects of the peptide, administered at the same dose, were evaluated in the same pigs during mechanical ventilation. Our results show that...
Article
In six anesthetized, paralyzed, mechanically ventilated pigs we evaluated the respiratory effects of inhaled nitric oxide (NO) (80 ppm in O2) under control conditions and after platelet-activating factor (PAF) administration (50 ng/kg, i.v.). PAF was also administered to the same pigs after pretreatment with indomethacin (3 mg/kg, i.v.). The mechan...
Article
Full-text available
The role of endogenous nitric oxide (NO) on vascular and respiratory smooth muscle basal tone was evaluated in six anaesthetized, paralysed, mechanically ventilated pigs. The involvement of endogenous NO in PAF-induced shock and airway hyperresponsiveness was also studied. PAF (50 ng/kg, i.v.) was ad