Mariana Radkova

Mariana Radkova
Agrobioinstitute · functional genetics

About

34
Publications
7,452
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182
Citations
Citations since 2017
24 Research Items
163 Citations
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2017201820192020202120222023010203040

Publications

Publications (34)
Article
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The general awareness of the threats on biodiversity and water quality raised the number of studies that use phytoplankton in assessment procedures. Since most metrics require obtaining mean values, this paper presents data that may help speed up field work and find indicators for a rapid water quality assessment based on single samplings, allowing...
Article
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Transcriptional factors play an essential role in the regulation of developmental processes in plants. GRAS family transcription factors are plant specific, and GRAS members possess multiple functions involved in plant growth and in response to abiotic and biotic stress. The members are divided into several subgroups. This study is focused on one p...
Article
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The paper presents the first proof of planktonic cyanoprokaryote genus Cuspidothrix as an anatoxin A (ATX) producer in Bulgarian wetlands. The results from polymerase chain reaction (PCR) obtained from two summer sampling campaigns in 26 selected lakes and reservoirs demonstrated presence of the anaC gene, responsible for ATX production in 21 strai...
Article
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We have witnessed a drastic increase in global fires in recent years that are strongly linked to climate change. On the other hand, the prescribed fires are applied in agricultural practice; however, their effectiveness is controversial. It is important to study the effects of both types of fires – wild and prescribed to predict their impact on bio...
Article
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The evaluation of climate plasticity and content of free amino acids, sugars and fatty acids in Bulgarian soybean cultivars were used as an innovative approach. The field performance, expression and metabolomic profiles of leaves, green seeds and mature seeds of plants grown from low temperature pre-treated and not-treated seeds were assessed by re...
Article
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Bulgaria, situated on the Balkan Peninsula, is rich in small and shallow, natural and man-made non-lotic waterbodies, which are threatened by blooms of Cyanoprokaryota/Cyanobacteria. Although cyanotoxins in Bulgarian surface waters are receiving increased attention, there is no information on microviridins and their producers. This paper presents r...
Article
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We studied the diversity of ground beetles in a typical potato-growing region of South-Western Bulgaria for three consecutive years (2013–2015). The aim was to discuss the present data in relation to results obtained about ten years earlier for the same agricultural area. Totally, 2058 individuals of 45 species were recorded during the period of th...
Article
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Hemocyanins are copper-binding proteins that play a crucial role in the physiological processes in crustaceans. In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach. The full-length cDNA of EvHc5 was 2254...
Article
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The summer phytoplankton structure of ten Bulgarian waterbodies was studied by HPLC analysis of marker pigments, light microscopy (LM) and PCR amplification of mcyB and mcyE gene sequences. The aim was to detect biodiversity and spread of toxigenic strains of potential microcystin producers and the important bloom-forming genus Microcystis in parti...
Article
Zinc finger proteins bind nucleic acids or act in transcriptional or translational regulation. The present study aimed to explore the effect of heterologous expression of the Medicago truncatula gene (Mt-Zn-CCHC), which encodes a Zinc finger CCHC type protein, in Arabidopsis thaliana. The Mt-Zn-CCHC gene, which affects seed size in M. truncatula, w...
Article
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The rising interest in harmful cyanoprokaryote blooms promotes an increase of phycological and ecological research on potentially toxic species and their hazardous substances. The present study aimed to identify the main microcystin (MC) producers and their contribution to the phytoplankton of shallow waterbodies in Bulgaria, applying different met...
Article
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p> Technological qualities of the seeds, including their mass, play an important role in the purposeful use of soybean for food production. The purpose of this study is to determine the potential of specific crosses and recombinant lines in the combinative breeding of high yielding large-seeded soybean varieties. During the period of 2018-2019 the...
Article
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This paper presents results from the first application of molecular-genetic analysis combined with conventional light microscopy (LM) for identification of cylindrospermopsin (CYN) producers in field phytoplankton samples from Bulgaria. In total 68 cyanoprokaryotes were found by LM, out of which 18 were considered potential CYN-producers according...
Article
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The paper presents results from the first application of polyphasic approach in studies of field samples from Bulgaria. This approach, which combined the conventional light microscopy (LM) and molecular-genetic methods (based on PCR amplified fragments of microcystin synthetase gene mcyE), revealed that almost all microcystin-producers in the studi...
Article
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The results from the investigation of the phytoplankton in nine shallow wetlands, situated in Central and Eastern Bulgaria, conducted in the frame of recent projects related to identifying harmful algal blooms and algal toxins in the country are demonstrated. Therefore, the focus is on the cyanoprokaryotes and their toxigenic species in particular....
Article
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In the frames of F7P project AMIGA "Assessing and monitoring the impact of genetically modified plants on agro-ecosystems" we studied the biodiversity of epigeic spiders in a typical potato growing region of Southwestern Bul-garia for three consecutive years (2013-2015). A total of 1523 individuals from 46 species were collected and defined. The pr...
Presentation
Full-text available
The cyanoprokaryotes are common inhabitants of water bodies but when developed in massive they are capable of harming wetlands with serious economic damage and consequences for human and animal health. Therefore, their low-cost monitoring with reliable identification is increasingly important. Bulgaria is not an exception in this case and cyanoprok...
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This review provides data on the possibilities for commercial use of algae of the green streptophyte genus Klebsormidium. It is one of the basal algal progenitors of land plants and shows many mechanisms and features which allow its adaptation to different environmental stresses associated with land colonization. This has led to formation of struct...
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Today’s global problems and challenges have given rise to a new field of interest – bioeconomics. It is strongly related to phycoprospecting, or searching for specific algal strains of commercial importance. There has been growing interest in the small algal class Eustigmatophyceae in recent years. These microscopic stramenopilic algae, which have...
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Legumes are one of the world's leading sources of nutrition, providing sustainable agriculture. They require minimal amounts of soil improvers (fertilizers) due to the ability to absorb nitrogen through symbiotic interactions with nitrogen-fixing soil microorganisms. The trend for human population growth requires an adequate growth in crop producti...
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Recently, scientists are focused on the discovery of alternative sources of high-value products such as proteins, lipids and carbohydrates. Microalgal cells have great potential for accumulation of bioactive components important in food, feed, pharmaceutical and cosmetics industry, as sources of biofuel, for waste water remediation, etc. The main a...
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Genetically modified (GM) plants may show unintended differences compared to the original varieties, due to the modification process. Such differences might in some cases affect non-target organisms linked to the crop into an agro-ecosystem. In this paper, we aimed to study interactions of two blight-resistant GM potato clones with the aphid specie...
Article
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In the frames of the F7P Project “Assessing and monitoring the impact of genetically modified plants on agro-ecosystems, (AMIGA)”, the partner from Bulgaria was involved in monitoring and collecting data describing the arthropods communities, representative for five biogeographical regions of Europe. Our investigations were focused on beneficial a...
Article
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One of the main subjects of the AMIGA (Assessing and Monitoring the Impacts of Genetically modified plants on Agro-ecosystems) project of F7P was to establish protocols for risk assessment in GM crops providing reliable evaluation of their impact on the environment. We were able to develop an efficient bioassay where micropropagated plants of cisge...
Article
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The Tnt1 retrotransposon of tobacco (Nicotiana tabacum) has proven to be a very efficient mutagen for the model legume Medicago truncatula ecotype 108 and cultivar Jemalong 2HA and for economically important plants, such as soybean and potato. In this study, the activity of Tnt1 in the model legume Lotus japonicus L. was tested. First, a new regene...
Article
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Arthropod hemocyanins (Hcs) are a family of large extracellular oxygen-transporting proteins with high molecular mass and hexameric or multi-hexameric molecular assembly. This study reports for the first time the isolation and characterization of the structure of an arthropod hemocyanin from crab Eriphia verrucosa (EvH) living in the Black Sea. Its...
Article
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A full length transcript encoding actin from the veined rapa whelk Rapana venosa (RvAct1) was isolated using RT-PCR and 5 /3 RACE. The cDNA spans 1565 bp and contains a long 3 untranslated region of 293 bp and 111 bp of 5 untranslated sequence. The open reading frame encodes a polypeptide of 376 amino acids. Sequence comparison showed that RvAct1 i...
Article
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Arthropod hemocyanins (Hcs) are a family of large extracellular oxygen-transporting proteins with high molecular mass and hexameric or multi-hexameric molecular assembly. This study reports the isolation and characterization of the structure of an arthropod hemocyanin from crab Cancer pagurus (CpH) living in the Black Sea. Its oligomeric quaternary...
Article
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Genetic variability in modern crops is limited due to domestication and selection processes. Genetic variation in eight Bulgarian tomato varieties and breeding lines (variety Plovdivska karotina, variety IZK Alya, L21 beta, L53 beta, L1140, L1116, L975, L984) differing in their morphological and biochemical composition was assessed using a highly e...
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The establishment of transgenic tomato (Solanum lycopersicum) lines expressing human acidic fibroblast growth factor (haFGF) was reported. A nonantibiotic selection involving the manA gene from E. coli that confers resistance to mannose was applied. The transgenic nature of four selected tomato lines cultivar Bela was proved with PCR analysis. The...
Article
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The present investigation outlines the possibilities for use of genic male sterility in tomato as a tool for pollen flow restriction. Three tomato lines (two genic male sterile; sporogenous controlled by gene ms 10 and functional, controlled by gene ps 2 as well as one fertile Ailsa Griag) were included in the study. Field experiments were performe...
Article
Cold shock domain (CSD) proteins, or Y-box proteins, are nucleic acid-binding proteins that are widely distributed from bacteria to higher plants and animals. Bacterial CSD proteins play an essential role in cold adaptation by destabilizing RNA secondary structures. WHEAT COLD SHOCK DOMAIN PROTEIN 1 (WCSP1) shares biochemical functions with bacteri...

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