Mariana Leguia

Mariana Leguia
Pontifical Catholic University of Peru | PUCP · Vicerrectorado de Investigacion

Ph.D.

About

77
Publications
6,904
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860
Citations

Publications

Publications (77)
Article
Full-text available
Over half the world’s population is at risk for viruses transmitted by Aedes mosquitoes, such as dengue and Zika. The primary vector, Aedes aegypti , thrives in urban environments. Despite decades of effort, cases and geographic range of Aedes -borne viruses (ABVs) continue to expand. Rigorously proven vector control interventions that measure prot...
Article
Despite several reports worldwide documenting the presence of Rickettsia asembonensis in samples derived from ectoparasites, animals and more recently humans, genomic information of these specimens remains scarce, and when available, is usually limited to small genomic fragments of limited value. We generated complete sequences for two conserved (1...
Preprint
Full-text available
Background: Over half the world's population is at risk for viruses transmitted by Aedes mosquitoes, such as, dengue and Zika. The primary vector, Aedes aegypti, thrives in urban environments. Despite decades of effort, cases and the geographic range of Aedes-borne viruses (ABV) continue to expand. To date, there are no rigorously proven vector con...
Article
Minority Gene Expression Profiling (MGEP) refers to a scenario where the expression profiles of specific genes of interest are concentrated in a small cellular pool that is embedded within a larger, non-expressive pool. An example of this is the analysis of disease-related genes within sub-populations of blood or biopsied tissues. These systems are...
Article
This brief report serves as an introduction to a supplement of the Journal of Infectious Diseases entitled "Next-Generation Sequencing (NGS) Technologies to Advance Global Infectious Disease Research." We briefly discuss the history of NGS technologies and describe how the techniques developed during the past 40 years have impacted our understandin...
Article
Full-text available
Rickettsia and Leptospira spp. are under-recognized causes of acute febrile disease worldwide. Rickettsia species are often placed into the spotted fever group rickettsiae (SFGR) and typhus group rickettsiae (TGR). We explored the antibody prevalence among humans for these two groups of rickettsiae in four regions of Peru (Lima, Cusco, Puerto Maldo...
Article
Full-text available
While studying respiratory infections in Peru, we identified Venezuelan equine encephalitis virus (VEEV) in a nasopharyngeal swab, indicating that this alphavirus can be present in human respiratory secretions. Because VEEV may be infectious when aerosolized, our finding is relevant for the management of VEEV-infected patients and for VEEV transmis...
Article
Full-text available
While studying rickettsial infections in Peru, we detected Rickettsia asembonensis in fleas from domestic animals. We characterized 5 complete genomic regions (17kDa, gltA, ompA, ompB, and sca4) and conducted multilocus sequence typing and phylogenetic analyses. The molecular isolate from Peru is distinct from the original R. asembonensis strain fr...
Data
Reference sequences used for phylogenetic analysis of Peru Rickettsia asembonensis molecular isolate.
Article
Full-text available
Background: The transmission dynamics of human metapneumovirus (HMPV) in tropical countries remain unclear. Further understanding of the genetic diversity of the virus could aid in HMPV vaccine design and improve our understanding of respiratory virus transmission dynamics in low- and middle-income countries. Materials & methods: We examined the...
Article
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Using a large, passive, febrile surveillance program in Iquitos, Peru, we retrospectively tested human blood specimens for scrub typhus group orientiae by ELISA, immu-nofluorescence assay, and PCR. Of 1,124 participants, 60 (5.3%) were seropositive, and 1 showed evidence of recent active infection. Our serologic data indicate that scrub typhus is p...
Article
Full-text available
We have developed methods for full-genome sequencing of Zika viruses (ZIKVs) based on a targeted amplification approach. We used alignments of publicly available complete genome data to design a primer set that selectively amplifies ZIKVs. The approach includes amplification strategies for templates present at both high- and low-copy number, and PC...
Article
Full-text available
The genus Bartonella contains >40 species, and an increasing number of these Bartonella species are being implicated in human disease. One such pathogen is Bartonella ancashensis, which was isolated in blood samples from 2 patients living in Caraz, Peru, during a clinical trial of treatment for bartonellosis. Three B. ancashensis strains were analy...
Article
Full-text available
Using a large, passive, clinic-based surveillance program in Iquitos, Peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. Blood specimens from humans and animals were assayed for spotted f...
Data
Univariate analysis comparing participants with co-infections to those without (DOCX)
Article
Full-text available
We report optimized workflows for full-genome sequencing of dengue viruses (DENVs) 1-4. Based on alignments of publicly available complete genomes we modified and expanded existing primers sets to amplify DENV genotypes that were previously difficult or impossible to sequence. We also report improvements to streamline laboratory handling, including...
Article
Full-text available
While studying respiratory infections of unknown etiology we detected Saffold virus in an oropharyngeal swab collected from a two-year-old female suffering from diarrhea and respiratory illness. The full viral genome recovered by deep sequencing showed 98% identity to a previously described Saffold strain isolated in Japan. Phylogenetic analysis co...
Article
Full-text available
Here we present the complete genome sequence of Bartonella ancashensis strain 20.00, isolated from the blood of a Peruvian patient with verruga peruana, known as Carrion’s disease. Bartonella ancashensis is a Gram-negative bacillus, phylogenetically most similar to Bartonella bacilliformis , the causative agent of Oroya fever and verruga peruana.
Article
Full-text available
There is an increasing role for bioinformatic and phylogenetic analysis in tropical medicine research. However, scientists working in low- and middle-income regions may lack access to training opportunities in these methods. To help address this gap, we offered a 5-day intensive bioinformatics workshop in Lima, Peru and present the syllabus here fo...
Article
Full-text available
Three Bartonella isolates were recovered from the blood of two patients enrolled in a clinical trial for the treatment of chronic stage Bartonella bacilliformis infection (verruga peruana) in Caraz, Ancash, Peru. The isolates were initially characterized by sequencing a fragment of the gltA gene, and found to be disparate from B. bacilliformis. The...
Data
##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. v2.5.3 Sequencing Technology :: Illumina ##Assembly-Data-END##
Article
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A recently described clinical isolate, “Candidatus Bartonella ancashi,” was obtained from a blood sample of a patient presenting with verruga peruana in the Ancash region of Peru. This sample and a second isolate obtained 60 days later from the same patient were molecularly typed using multilocus sequence typing (MLST) and multispacer sequence typi...
Data
##Assembly-Data-START## Assembly Method :: GSAssembler v. 2.5.3 Assembly Name :: 20.60 Coverage :: 73.2 Sequencing Technology :: 454; Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. 2.5.3 Assembly Name :: 20.60 Coverage :: 73.2 Sequencing Technology :: 454; Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. 2.5.3 Assembly Name :: 20.60 Coverage :: 73.2 Sequencing Technology :: 454; Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. 2.5.3 Assembly Name :: 20.60 Coverage :: 73.2 Sequencing Technology :: 454; Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. 2.5.3 Assembly Name :: 20.60 Coverage :: 73.2 Sequencing Technology :: 454; Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. 2.5.3 Assembly Name :: 20.60 Coverage :: 73.2 Sequencing Technology :: 454; Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Assembly Method :: GSAssembler v. 2.5.3 Assembly Name :: 20.60 Coverage :: 73.2 Sequencing Technology :: 454; Sanger dideoxy sequencing ##Assembly-Data-END##
Article
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There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite part...
Data
Basic parts used for BglBricks-based 2ab assembly of bi-cistronic operons of Mediator complex.
Data
Heat maps of protein concentration values obtained via indirect ELISAs. Maps include all possible tags and tagging combinations in order to visualize expression patterns for each epitope-tagged ORF in all 528 bi-cistronic operons. The top map illustrates patterns of expression for Med7 located in the first ORF position and should be read from left...
Data
Complete data set of protein concentration values obtained via indirect ELISAs from all 528 bi-cistronic operons.
Article
Full-text available
We describe discovery of two enteroviruses detected in nasopharyngeal samples obtained from subjects with respiratory disease in Peru. Phylogenetic analysis indicated that both viruses belong to a clade within the human enterovirus C species, which includes the recently characterized human enteroviruses 109 and 104. Members of this clade have under...
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##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
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##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Article
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A metamorphic life-history is present in the majority of animal phyla. This developmental mode is particularly prominent among marine invertebrates with a bentho-planktonic life cycle, where a pelagic larval form transforms into a benthic adult. Metamorphic competence (the stage at which a larva is capable to undergo the metamorphic transformation...
Data
Appendix 1 We identified and cloned the full length cDNA from the sea urchin histidine decarboxylase (HDC) gene based on the annotation present in NCBI (XP_789367). We then used the protein sequence for phylogenetic analysis and comparison with other animal HDCs as well as three dopa decarboxylase genes (Ddc). HDC from S. purpuratus clearly falls w...
Data
Appendix 2 Neighbor joining phylogenetic analysis of sea urchin histamine receptor predictions in comparison to human H1, H2, H3 and H4 receptor. We identified all histamine-receptor like genes from SpBase (http://sugp.caltech.edu/SpBase/) and aligned them with human H1, H2 and H3 receptor. Phylogenetic analysis suggests that all three receptor typ...
Article
The histamine H1 receptor is a seven transmembrane G protein-coupled receptor thought to participate in the release of calcium from intracellular stores during egg activation. Prior to fertilization, the receptor is present exclusively at the surface of the egg. Shortly after fertilization, however, the receptor is endocytosed. To follow this trans...
Article
The primary bottleneck in synthetic biology research today is the construction of physical DNAs, a process that is often expensive, time-consuming, and riddled with cloning difficulties associated with the uniqueness of each DNA sequence. We have developed a series of biological and computational tools that lower existing barriers to automation and...
Article
Full-text available
Synthetic biological systems are currently created by an ad-hoc, iterative process of specification, design, and assembly. These systems would greatly benefit from a more formalized and rigorous specification of the desired system components as well as constraints on their composition. Therefore, the creation of robust and efficient design flows an...
Article
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Standard biological parts, such as BioBricks parts, provide the foundation for a new engineering discipline that enables the design and construction of synthetic biological systems with a variety of applications in bioenergy, new materials, therapeutics, and environmental remediation. Although the original BioBricks assembly standard has found wide...
Article
The BglBricks standard has been developed as an alternative to the original XbaI/SpeI standard primarily to provide a solution to the issue of generating translational-fusion parts. When joined together in a standard assembly reaction, BglBricks parts contain a scar sequence of GGATCT between the two parts. This sequence conveniently translates as...
Article
The signaling networks controlling calcium release and cortical granule exocytosis at fertilization are complex and multilayered, providing various points for regulatory input and quality control. Though it is clear that many of the mechanisms leading to both calcium release and cortical granule exocytosis are conserved, a great deal of variability...
Article
Sperm fusion with the egg initiates a signaling cascade that releases intracellular calcium (Ca(i) (2+)) from the endoplasmic reticulum (ER). In sea urchins, Ca(2+) is released as a single, large transient via two distinct pathways. The first depends on inositol 1,4,5-triphosphate (IP(3)) production and triggers the initial phase of Ca(2+) release,...
Article
Cortical granules are stimulus-dependent secretory vesicles found in the egg cortex of most vertebrates and many invertebrates. Upon fertilization, an increase in intracellular calcium levels triggers cortical granules to exocytose enzymes and structural proteins that permanently modify the extracellular surface of the egg to prevent polyspermy. Sy...
Article
Regulated secretion is mediated by SNAREs (soluble NSF attachment receptors) and their regulators and effectors, which include the SM (sec1/munc18) family of proteins. Homologs of the SNAREs have been identified in sea urchins, associated with cortical granule exocytosis at fertilization, with membranes of the cleavage furrow, and in secretory cell...