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Mariana Boadella

Mariana Boadella
SABIOTEC

DVM, PhD, MBA

About

99
Publications
20,038
Reads
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2,185
Citations
Introduction
What I work on now: I work on the improvement of wildlife monitoring through the development of diagnostic tools for wildlife, and I also work on the development of disease control methods at the wildlife-domestic-human interface. But my challenge now is to reinvent my concept of making science. I want to open a bridge between us (researchers) and the “business world”; I also want to open our research to the general public; and finally, I’ll work to find new ways to finance the science we do.
Additional affiliations
March 2015 - present
SABIOTEC
Position
  • CEO
Description
  • SABIOTEC is dedicated to contribute to health, animal production and conservation through transfer knowledge.
June 2014 - February 2015
Erasmus MC
Position
  • Marie Curie Fellow
June 2014 - November 2015
SABIOtec
Position
  • CEO
Education
September 2007 - December 2011
University of Castilla-La Mancha
Field of study
  • Wildlife disease trends

Publications

Publications (99)
Article
Full-text available
Many people worldwide suffer from hepatitis C virus (HCV) infection, which is frequently persistent. The lack of efficient vaccines against HCV and the unavailability of or limited compliance with existing antiviral therapies is problematic for health care systems worldwide. Improved small animal models would support further hepacivirus research, i...
Article
Full-text available
Wildlife management and conservation requires monitoring of species distribution and population indicators, especially when the unbalanced demographic changes of some species can affect the whole ecosystem functioning. The populations of wild boar (Sus scrofa) have, over the past few decades, undergone an expansion around the world, reaching situat...
Article
Full-text available
The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative)...
Article
Animal tuberculosis (TB), which is caused by the Mycobacterium tuberculosis complex (MTC), is a zoonotic disease of global concern, and has a wide variety of wild and domestic reservoirs that can establish complex epidemiological systems. Of all the strategies employed to control TB, reducing the risks of interaction at the wildlife-livestock inter...
Article
Full-text available
Attaining and maintaining the Official Tuberculosis Free status continues to be a challenge when several domestic and wild hosts contribute to the maintenance of the Mycobacterium tuberculosis complex (MTC). Local tuberculosis hotspots are sometimes identified in cattle in low-prevalence regions. We have, therefore, studied one such hotspot in dept...
Article
Mycobacterial diseases are important health issues in farmed deer. The single intradermal tuberculin test is the standard test for tuberculosis testing in deer. We studied two factors which might influence the response of deer to skin testing: the inoculation site and the injection device. Deer included in this study were 2.5 years old farmed red d...
Article
Full-text available
The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite considered as the major pest in the egg-laying industry. Its pesticide-based control is only partially successful and requires the development of new control interventions such as vaccines. In this study, we follow a vaccinology approach to identify PRM candidate prot...
Article
Full-text available
In recent years, outbreaks caused by multi-host pathogens (MHP) have posed a serious challenge to public and animal health authorities. The frequent implication of wildlife in such disease systems and a lack of guidelines for mitigating these diseases within wild animal populations partially explain why the outbreaks are particularly challenging. T...
Article
Full-text available
The red deer (Cervus elaphus) is a widespread wild ungulate in Europe that has suffered strong anthropogenic impacts over their distribution during the last centuries, but also at the present time, due its economic importance as a game species. Here we focus on the evolutionary history of the red deer in Iberia, one of the three main southern refug...
Data
Mitochondrial D-loop phylogeography. The red deer mitochondrial D-loop haplotypes (329 bp) included in the phylogeographic analysis at a European level. (XLSX)
Data
Mitochondrial D-loop phylogeography. Mitochondrial D-loop similarity between the red deer haplotypes found in the present study and those reported by Niedziałkowska et al. [84]. For this comparison a 248 bp fragment size was considered. (DOCX)
Data
Species distribution modelling. The bioclimatic variables used to study the climatic requirements of Cervus elaphus distribution throughout western Europe and North Africa. A ‘quarter’ refers to a fraction of the year (i.e. three months). The same variables are available for present (1950–2000), Mid-Holocene (6 ky BP), Last Glacial Maximum (22 ky B...
Data
Population demography and divergence inferred by the program DIYABC using microsatellite data. Posterior parameter estimates (median and 95% confidence intervals) for the best-supported scenario calculated using 1% of simulated datasets closest to the observed values. Simulations and approximate Bayesian computation analyses were performed includin...
Data
Species distribution modelling. Climatic suitability for the occurrence of Cervus elaphus in western Europe and North Africa during the Mid-Holocene (6 ky BP), Last Glacial Maximum (LGM, 22 ky BP) and interglacial period (120 ky BP) represented for the generalized boosting model (GBM), classification tree analysis (CTA) and the ensemble of their fo...
Data
Archaeological data and species distribution model for red deer during the LGM. Map showing the geographic distribution of the red deer fossil records dated within the period of Last Glacial Maximum (Fig 9) and the climatic suitability for occurrence of red deer at 22 kyBP (Fig 8), predicted according to the climatic niche for the species determine...
Data
Mitochondrial D-loop phylogeography. Mitochondrial D-Loop similarity between the red deer haplotypes found in the present study and those reported by Meiri et al. [15]. For this comparison a 316 bp fragment size was considered, which after excluding nucleotide sites with gaps and missing data resulted in a total of 180 nucleotide sites analysed. (D...
Data
Mitochondrial D-loop phylogeography. Mitochondrial D-Loop similarity between the red deer haplotypes found in the present study and those reported by Rey-Iglesia et al. [37]. For this comparison a 670 bp fragment size was considered, which after excluding nucleotide sites with gaps and missing data resulted in a total of 449 nucleotide sites analys...
Data
Analysis of population structure. Plots showing the results for both the Bayesian clustering analyses conducted in STRUCTURE software (see Figs 3A and 4A and S4 Fig) and the highest ΔK value obtained following Evanno et al. [59] procedures. (DOCX)
Data
Isolation-by-distance in the Iberian red deer populations. Plots showing the relationship between genetic distance [pairwise FST/(1-FST)] and geographic distance (log km) between the Iberian red deer populations quantified for both microsatellites and mitochondrial datasets (isolation-by-distance). (DOCX)
Data
Population structure of the European red deer populations. Bayesian clustering analyses performed in STRUCTURE [56] on microsatellite data from the central and north European red deer populations, considering the best ΔK values obtained following Evanno et al. [59] procedures (see also S1 Fig). Proportional membership to each cluster is indicated f...
Data
Mitochondrial D-loop phylogeography of red deer. Median joining network showing the evolutionary relationship of D-loop haplotypes found in this study (670 bp fragment size) for the red deer in Europe. Numbers within circles correspond to haplotype name described in the main manuscript for the 329 bp haplotypes (see details in S1 Table). A bar on e...
Data
Mitochondrial D-loop phylogeography. Mitochondrial D-Loop similarity between the red deer haplotypes found in the present study and those reported by Stanton et al. [26]. For this comparison a 328 bp fragment size was considered, which after excluding nucleotide sites with gaps and missing data resulted in a total of 264 nucleotide sites analysed....
Data
Pairwise FST values for both microsatellite (above diagonal) and mitochondrial (below diagonal) datasets for the red deer populations studied. Population codes are described as in Fig 1 of the main manuscript. (DOCX)
Data
European red deer differentiation inferred using nuclear and mitochondrial markers. Neighbor-joining trees representing the genetic differentiation among populations measured as pairwise FST for both microsatellite (left) and mtDNA (right) datasets. Population codes are described in Fig 1 of the main manuscript. (DOCX)
Data
Mitochondrial D-loop evolutionary rate. Ancient DNA mitochondrial D-loop sequences used for the inference of within-species evolutionary rate. (DOCX)
Data
Mitochondrial D-loop evolutionary rate. Bayesian MCMC analysis performed to estimate the evolutionary rate of the D-loop mitochondrial fragment studied, using calibrated fossil ages of red deer from Europe (see S6 Table). Results of independent runs were combined using TRACER, version 1.5 [87], the final result was given based on average Log10 of t...
Data
Species distribution modelling. Results of the generalized linear model (GLM) model developed for the current distribution of Cervus elaphus in western Europe and North Africa. Predictors are listed following the order of inclusion in a stepwise procedure (the first one on top). The coefficient and its standard error (SE) and z-value test statistic...
Data
Species distribution modelling. Results of the generalized additive model (GAM), generalized boosting model (GBM), classification tree analysis (CTA), artificial neural network (ANN), flexible discriminant analysis (FDA) and, in addition, an ensemble of their forecasts, developed on the current distribution of Cervus elaphus in western Europe and N...
Data
Population structure in the Iberian red deer. Number of individuals excluded from the Bayesian STRUCTURE analysis at the Iberian level, using microsatellite data. Exclusion was based on the individuals that had a membership proportion (qi) from the Iberian cluster lower than 95% in the main STRUCTURE analysis (all European populations) and in the E...
Data
Species distribution modelling. Calibration plot showing the relationship between the predicted probability for red deer occurrence according to its climatic niche and the observed frequency of the species in the validation dataset. Open symbols indicate bins with < 15 localities, in which the frequency observed should be considered with caution [1...
Article
Livestock farming is a common human activity that not only modifies natural habitat but may also lead to interactions with other wild animal species. We studied whether health status of roe deer (Capreolus capreolus) is influenced by density, livestock and type of habitat. We analyzed 208 samples (120 faecal and 88 sera) from roe deer populations o...
Article
Full-text available
El Ácaro Rojo de las Gallinas (ARG) o Dermanyssus gallinae es el principal ectoparásito que afecta a gallinas ponedoras. Este ácaro se oculta durante el día en grietas y pequeños es-pacios fuera del alcance de las aves y es, gene-ralmente, durante la noche cuando se alimenta de la sangre de las gallinas. Este parásito tiene serias repercusiones en...
Conference Paper
The Poultry Red Mite (Dermanyssus gallinae) is the most relevant ectoparasite in layer farms, and highly prevalent in European farms. This situation is a serious challenge for the poultry industry as the biology of the Poultry Red Mite (PRM) and the lack of effective tools make its control complicated. Depending on the level of infestation, PRM cau...
Poster
Full-text available
Poultry Red Mites (PRM) pose a threat to the egg industry worldwide. It causes serious economic losses due to reduction in egg production, higher costs (treatments and prevention), potential disease transmission and hens welfare. In addition, producers are facing the lack of effective control tools because of the development of resistance against s...
Article
Species distribution models based on occurrence data (SDM) are used to derive broad-scale estimates of relative abundance, but a triangular relationship between predicted suitability for species occurrence and abundance is commonly observed—that is, a low suitability would correspond to low abundances, whereas a high suitability would correspond to...
Article
Full-text available
Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic...
Article
Full-text available
Background During the last decade, the spread of many flaviviruses (Genus Flavivirus) has been reported, representing an emerging threat for both animal and human health. To further study utility of wild ruminant samples in West Nile virus (WNV) surveillance, we assessed spatio–temporal trends and factors associated with WNV and cross-reacting flav...
Conference Paper
Full-text available
Extensive livestock farming is an important component of the landscape and a primary sector in south-central Spain. In this type of farms, water and food resources are scarce for a long period along the year, which conduct to wildlife (especially wild boar and red deer) and domestic livestock to share resources. Proper management of these interacti...
Article
In 2012, a wild boar (Sus scrofa) tuberculosis (TB) control programme was set up in a wild boar farm by means of intramuscular (IM) vaccination with a heat-inactivated Mycobacterium bovis vaccine (IV). The goal was to assess safety and efficacy of the parenterally administered IV in a large farm setting with natural M. bovis circulation. Based on p...
Article
Full-text available
Ticks are vectors of diseases that affect humans and animals worldwide. Tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. Recently, the Rhipicephalus microplus Subolesin (SUB)-Anaplasma marginale MSP1a chimeric antigen was produced in Escherichia coli as membrane-bound and exposed protein a...
Article
Full-text available
The control of multi-host pathogens such as Coxiella burnetii should rely in accurate information on the roles played by main hosts. We aimed to determine the implication of the red deer (Cervus elaphus) in the ecology of C. burnetii. We predicted that red deer populations from wide geographic areas within a European context would be exposed to C....
Article
Animal tuberculosis (TB) caused by infection with Mycobacterium bovis and closely related members of the M. tuberculosis complex (MTC), is often reported in the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against MTC antigens are valuable tools for TB monitoring and control in suids. However, only limited knowledge exists on serolog...
Article
Autochthonous human Hepatitis E virus (HEV) infections in industrialized countries (due to genotypes 3 and 4) are increasingly reported and are linked to zoonotic transmission, mainly through consumption of contaminated meat from pigs, Eurasian wild boar (Sus scrofa) and deer. Wild boar is deemed as the main HEV wildlife reservoir in these countrie...
Article
Full-text available
The control of diseases shared with wildlife requires the development of strategies that will reduce pathogen transmission between wildlife and both domestic animals and humans. This review describes and criticizes the options currently applied and attempts to forecast wildlife disease control in the coming decades. Establishing a proper surveillan...
Conference Paper
Full-text available
The implication of wildlife in Coxiella burnetii - the causal agent of Q fever - ecology has been largely dismissed. Changes in red deer population dynamics may change their relevance as C. burnetii reservoirs for livestock and/or humans. We analysed the rate of exposure to C. burnetii and shedding, and investigated the clinical outcome of Q fever...
Chapter
This chapter addresses (1) the distribution and abundance trends of key wildlife hosts of food-borne zoonotic infections in Europe; (2) some representative case-studies regarding food-borne zoonoses shared with wildlife, with particular regard to the role of direct and indirect human-livestock-wildlife interactions; and (3) the efforts required to...
Article
Full-text available
Mycobacterium bovis causes animal tuberculosis (TB) in cattle, humans, and other mammalian species, including pigs. The goal of this study was to experimentally assess the response of pigs with and without tonsillectomy to oral vaccination with heat-inactivated M. bovis and challenge with a virulent M. bovis field strain; to compare pig and wild bo...
Article
Full-text available
Individuals in natural populations are exposed to a diversity of pathogens which results in coinfections. The aim of this study was to investigate the relation between natural infection with tuberculosis (TB) due to infection by bacteria of the Mycobacterium tuberculosis complex and porcine circovirus type 2 (PCV2) in free-ranging Eurasian wild boa...
Article
Full-text available
Field vaccination trials with Mycobacterium bovis BCG, an attenuated mutant of M. bovis, are ongoing in Spain, where the Eurasian wild boar (Sus scrofa) is regarded as the main driver of animal tuberculosis (TB). The oral baiting strategy consists in deploying vaccine baits twice each summer, in order to gain access to a high proportion of wild boa...
Article
Full-text available
Tuberculosis (TB) remains a pandemic affecting billions of people worldwide, thus stressing the need for new vaccines. Defining the correlates of vaccine protection is essential to achieve this goal. In this study, we used the wild boar model for mycobacterial infection and TB to characterize the protective mechanisms elicited by a new heat inactiv...
Article
Full-text available
The number of pathogens known to infect humans is ever increasing. Whether such increase reflects improved surveillance and detection or actual emergence of novel pathogens is unclear. Nonetheless, infectious diseases are the second leading cause of human mortality and disability-adjusted life years lost worldwide [1,2]. On average, three to four n...
Chapter
In the Mediterranean part of the Spanish mainland, animal TB is still a major concern. This chapter summarizes the current knowledge on TB in cattle, other domestic M. tuberculosis complex (MTBC) hosts, and wildlife in Spain. It briefly addresses aspects of strain diversity and human infection, and provides an outlook on current and future research...
Article
The influences of management practices and past demographic history on genetic diversity are of critical relevance to sustainable practices and the conservation of wildlife populations. The red deer (Cervus elaphus) is an interesting model species to address these questions because it has a wide geographical distribution and it has been intensively...
Article
Full-text available
[Utilisation d'un outil sérologique pour estimer l'exposition des Sangliers À M. Bovis (article in French)] The Eurasian wild boar (Sus scrofa) is increasingly considered as a significant actor in the epidemiology of animal tuberculosis (TB). Therefore, monitoring TB in wild boar becomes a key tool in the development of comprehensive control scheme...
Article
Tuberculosis (TB), a chronic disease caused by infection with the Mycobacterium tuberculosis complex, is endemic in wild boar (Sus scrofa) and red deer (Cervus elaphus) in south-central Spain. Understanding the temporal dynamics of this chronic infection requires long time series data collection over large areas. The aim of this paper was to identi...
Article
Full-text available
The Eurasian wild boar (Sus scrofa) is increasingly considered as a relevant actor in the epidemiology of animal tuberculosis (TB). Therefore, monitoring TB in wild boar becomes a key tool for establishing comprehensive control schemes for this disease. To estimate the exposure of free living wild boar to Mycobacterium tuberculosis complex (MTC) in...
Article
Full-text available
Infections with Mycobacterium bovis and closely related members of the Mycobacterium tuberculosis complex (MTC) are shared between livestock, wildlife and sporadically human beings. Wildlife reservoirs exist worldwide and can interfere with bovine tuberculosis (TB) eradication efforts. The Eurasian wild boar (Sus scrofa) is a MTC maintenance host i...
Article
Full-text available
Armenteros, J.A., Barasona, J.A., Goadella, M. Acevedo, P., Gortazar, C., Vicente, J. (2013). A proposal to consider animal health aspects in the regulation of game management. Ecosistemas 22(2):54-60. Doi.: 10.7818/ECOS.2013.22-2.09 Game species share diseases with humans, livestock and wildlife, hence their relevance. However, game species health...
Article
The Canary Islands contain the most important dromedary camel (Camelus dromedarius) population in the European Union and are the main export point of dromedaries to continental Europe and Latin America. We investigated the presence of antibodies against relevant disease agents in 100 Canarian camel sera. Selected blood samples of the same animals w...
Article
Full-text available
Anaplasma phagocytophilum infects a wide variety of hosts and causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever in ruminants. Infection with A. phagocytophilum results in the modification of host gene expression and immune response. The objective of this research was to characterize gene expression in pigs (Sus scrofa...
Article
Full-text available
TO THE EDITOR: Intense wildlife management for hunting affects risks associated with zoonotic pathogens (1). White-tailed deer (Odocoileus virginianus) are increasingly managed by fencing, feeding, watering, and translocation to increase incomes from hunting in northern Mexico (2). These deer also play a major role in dissemination and reintroducti...
Conference Paper
Full-text available
Wildlife disease monitoring consists in the systematic recording of epidemiological data, with no other specific purpose than detecting temporal trends. Ideally, disease data should integrate with data on host abundance and distribution but unfortunately, this information is often lacking or fragmentary. As a noteworthy exception to this role, we p...
Conference Paper
Full-text available
Worldwide there is a need to develop strategies to control relevant infectious disease agents, such as Mycobacterium bovis and Aujeszky’s disease virus (ADV) in wildlife reservoirs. We describe observational data on the effects of Eurasian wild boar (Sus scrofa) abundance reduction of approximately 50%, through increased culling on the maintenance...