Maria Luisa Villarreal

Maria Luisa Villarreal
Universidad Autónoma del Estado de Morelos · Centre of Biotechnological Research (CEIB)

PhD

About

121
Publications
32,785
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2,566
Citations
Citations since 2016
23 Research Items
1262 Citations
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200

Publications

Publications (121)
Article
Carrot (Daucus carota) cells have been used to effectively manufacture recombinant biopharmaceuticals such as cytokines, vaccines, and antibodies. We generated the carrot cell line Z4, genetically modified to produce the LTB-Syn antigen, which is a fusion protein proposed for immunotherapy against synucleinopathies. In this work, the Z4 cell suspen...
Preprint
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The genus Galphimia is widely distributed in Mexico, and is represented by 22 species, including medicinal species. The sedative and anti-inflammatory effects of galphimines produced by the species Galphimia glauca have been documented. Formerly, molecular studies using DNA barcodes demonstrated that nine populations botanically classified as Galph...
Article
Medicinal plants have been used for thousands of years and nowadays are extensively employed to treat diverse illnesses. The increasing consumption of medicinal plants and its derivative products has led to an increase of adulterant material during the manufacturing process. The quality of the medicinal active products could be affected by reducing...
Article
Galphimia glauca Cav., Malpighiaceae, is used as a tranquilizer in Mexico. Its active compounds are nor-secofriedelane triterpenes known as galphimines. Using metabolic profiling, we previously demonstrated that individuals obtained in two localities out of the seven analyzed, exhibited accumulation of galphimines. In the present work, we tested th...
Article
Endophytic fungi represent a promising biotechnological tool to identify and produce in large scale novel anti-inflammatory bioactive compounds. In this study, Crescentia alata Kunth was selected following the ethnomedical criteria and a total of 219 isolates grouped in 86 morphotypes were obtained. From these, 44 isolates that presented a pigment-...
Article
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Ten Hyptis suaveolens hairy root lines were established by infecting nodal explants with K599+pGus-GFP+ and ATCC15834+pTDT strains from Agrobacterium rhizogenes. Genetic transformation was confirmed by epifluorescence and plagiotropic hairy root growth in absence of growth regulators. Cytotoxicity was determined using the sulforhodamine B method, a...
Article
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Highly prized huperzine A (Hup A), a natural alkaloid formerly isolated from the Chinese medicinal plant Huperzia serrata, has been widely used for the treatment of Alzheimer disease, inspiring us to search for endophytic fungi that produce this compound. In this study, we obtained the C17 fungus isolate from the Mexican club moss Phlegmariurus tax...
Article
Full-text available
Ten Hyptis suaveolens hairy root lines were established by infecting nodal explants with K599+pGus-GFP+ and ATCC15834+pTDT strains from Agrobacterium rhizogenes. Genetic transformation was confirmed by epifluorescence and plagiotropic hairy root growth in absence of growth regulators. Cytotoxicity was determined using the sulforhodamine B method, a...
Article
Full-text available
Galphimia glauca is a plant that is endemic to Mexico and has been commonly used since pre-Hispanic times to treat various illnesses, including central nervous system disorders and inflammation. The first studies investigating a natural population of G. glauca in Mexico showed that the plant has anxiolytic and sedative activities in mice and humans...
Article
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em> Bursera copallifera (DC) Bullock (Burseraceae) is a plant species used in folk medicine for the treatment of inflammation (bronchitis, cough). The dichloromethane-methanol extract (DML) of B. copallifera leaves was found to possess important antiproliferative and anti-inflammatory activities. In order to further correlate these activities with...
Article
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Microbes are tiny forms of life that can be found everywhere. The microbes that live inside healthy plants are commonly known as endophytes. Plants live together with their endophytes in symbiosis, which means that they work together to help each other out. Plants give shelter and food to their microbial guests, and in exchange, their microbial hel...
Article
Full-text available
Galphimia glauca (Cav.) Kuntze is an important endemic plant species, which possesses many medicinal properties and has been used in the Mexican traditional medicine for its sedative, anxiolytic, anticonvulsant, antiasthmatic and antiallergic properties. The therapeutic properties of this plant are mainly due to the presence of diverse bioactive co...
Article
Full-text available
Galphimia glauca (Cav.) Kuntze is an important endemic plant species, which possesses many medicinal properties and has been used in the Mexican traditional medicine for its sedative, anxiolytic, anticonvulsant, antiasthmatic and antiallergic properties. The therapeutic properties of this plant are mainly due to the presence of diverse bioactive co...
Article
Full-text available
Huperzine A (Hup A), the alkaloid produced by the Chinese medicinal plant Huperzia serrata, has been documented to be a promising agent for the treatment of Alzheimer's disease due to its potent acetylcholinesterase inhibitory (AChEI) activity. The search for anticholinesterase natural products, as well as for alternative sources of Hup A in Mexica...
Article
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Background Hippocratea celastroides Kunth, commonly known as “cancerina”, is used in Mexican Traditional Medicine for the treatment of gastric and intestinal infections, systemic and skin inflammation, injuries and gastritis. The aim of this research was to assess the anti-Helicobacter pylori activities of hydro-ethanolic root-bark extracts from Hi...
Article
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Despite prevention and treatment options, breast cancer (BC) has become one of the most important issues in the present day. Therefore, the need for more specific and efficient compounds remains paramount. We evaluated four previously isolated aryltetralin lignans: 5'-demethoxy-β-peltatin-A-methylether (1), acetylpodophyllotoxin (2), 5'-demethoxyde...
Article
There is much interest in the economic use of Agave tequilana Weber leaves to control major agro-industrial pests, because an estimated 741,000 tons of leaves are wasted each year as a byproduct of the tequila industry. Hexane and ethyl acetate extracts and the insecticidal activity of juice from the leaves of A. tequilana were evaluated against si...
Article
Ternstroemia pringlei (Rose) Standl. (Theaceae) is widely used in Mexican traditional medicine to treat a diverse array of illnesses including rheumatoid pains, and is listed as one of the most consumed medicinal plants in the country. We selected T. pringlei given the strong relationship between oxidative stress and arthritis pathology, and invest...
Article
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The cytotoxic activity and the chemical composition of the dichloromethane/methanol root extract of Linum scabrellum Planchon (Linaceae) were analyzed. Using NMR spectra and mass spectrometry analyses of the extract we identified eight main constituents: oleic acid (1), octadecenoic acid (2), stigmasterol (3), α-amyrin (4), pinoresinol (5), 6 metho...
Data
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Supplementary Material provides all the spectroscopic data of isolated compounds (NMR and MS spectra).
Conference Paper
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Desde tiempos prehispánicos la especie mexicana Galphimia glauca (Cav.) Kuntze (Malpighiaceae), conocida como “calderona amarilla”, ha sido empleada en el tratamiento de desórdenes del sistema nervioso central y de otras enfermedades [1]. De sus extractos se han aislado diferentes compuestos que confieren a la planta actividades anti-alergénica in...
Article
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Three new aryldihydronaphthalene-type lignans, namely, 7′,8′-dehydropodophyllotoxin (1); 7′,8′-dehydro acetylpodophyllotoxin (2); and 7′,8′-dehydro trans-p-cumaroylpodophyllotoxin (3), were isolated from the stem bark of Bursera fagaroides var. fagaroides (Burseraceae), together with six known lignans, podophyllotoxin (4), acetylpodophyllotoxin (5)...
Article
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Abstract. The wild shurb Hyptis suaveolens also known as “chia” was used as food and medicine since prehispanic times in Mexico, and nowadays is employed to treat several ailments. The anticancer lignan podophyllotoxin (PTOX) was recently reported in this plant, and its highest accumulation occurs in roots. This work reports the establishment of se...
Article
Full-text available
Hippocratea celastroides is used in Mexican Traditional Medicine for the treatment of gastric and intestinal infections, systemic and skin inflammation, injuries and gastritis. A methanolic (MeOH) extract was prepared from the root-bark, then subjected to an acid-base extraction obtaining the total alkaloid and the non-alkaloid extracts. Ear edema...
Article
Phloroglucinols display interesting biological activities such as antiproliferative, antidepressant and antibacterial. The occurrence of phloroglucinols is restricted to ferns of the Dryopteridaceae family. The aim of the present study was to identify new sources of bioactive phloroglucinols from Mexican ferns. The rhizomes hexanic extracts from El...
Article
Ternstroemia pringlei is a medicinal plant widely distributed in Mexico. Since, a series of antioxidative phenylethanoid glycosides have been isolated in the Asiatic species T. japonica [1], we decided to study the antioxidative action of T. pringlei extracts obtained from various organs, and to investigate the presence of phenyethnanoid glycosides...
Article
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Hyptis suaveolens was an important source of food and medicines in pre-hispanic Mιxico and is actually used popularly to treat respiratory and skin diseases, fever, pain, and cramps, between other ailments. In 2008 the presence of podophyllotoxin (PTOX) was reported in this plant. To establish in vitro cultures of H. suaveolens able to produce PTOX...
Article
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The hydroalcoholic extract of the steam bark of B. fagaroides var. fagaroides displayed potent cytotoxic activity against four cancer cell lines, namely KB (ED50 = 9.6 × 10(-2) μg/mL), PC-3 (ED50 = 2.5 × 10(-1) μg/mL), MCF-7 (ED50 = 6.6 μg/mL), and HF-6 (ED50 = 7.1 × 10(-3) μg/mL). This extract also showed anti-tumour activity when assayed on mice...
Article
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Bougainvillea glabra C., Eucalyptus globules Labill., and Gnaphalium attenuatum DC., as well as propolis, have been used in Mexican traditional medicine as a remedy to treat respiratory illnesses. There are few biological reports of such material collected in Mexico, despite the high demand for them as raw material for popular and industrial uses....
Article
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Ethnopharmacology relevance: Galphimia glauca (Malpighiaceae) is a Mexican plant popularly used as a tranquilizer in the treatment of nervous system disorders, although it is also used to treat other common illnesses. Aim of the study: The aim of this investigation is to find out if populations of Galphimia glauca collected in different regions...
Article
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The aim of this review is to provide a summary on multidisciplinary scientific information obtained from medicinal plants used worldwide to treat anxiety, focusing on pharmacological and clinical studies. The bibliographical investigation was carried out by consulting five peer-reviewed worldwide database publications for references, and patents. T...
Article
Full-text available
Galphimia glauca has a long traditional use, and continues to be used in Mexico as a natural tranquilizer for the treatment of Central Nervous System disorders as well as for other illnesses. In 2005 the initial use of metabolic profiling to populations of Galphimia glauca resulted in two of the six collected populations being producers for galphim...
Article
Full-text available
Abstract: The aim of this review is to provide a summary on multidisciplinary scientific information obtained from medicinal plants used worldwide to treat anxiety, focusing on pharmacological and clinical studies. The bibliographical investigation was carried out by consulting five peer-reviewed worldwide database publications for references, and...
Article
Full-text available
The hydroalcoholic extract of the steam bark of B. fagaroides var. fagaroides displayed potent cytotoxic activity against four cancer cell lines, namely KB (ED50 = 9.6 × 10−2 μg/mL), PC-3 (ED50 = 2.5 × 10−1 μg/mL), MCF-7 (ED50 = 6.6 μg/mL), and HF-6 (ED50 = 7.1 × 10−3 μg/mL). This extract also showed anti-tumour activity when assayed on mice inocul...
Article
Full-text available
From the stem bark of Mangifera indica, seven cycloartane-type secondary metabolites were isolated. Compound 1 has been isolated for the first time from M. indica, whereas compounds 2 (2a and 2b, as an epimeric mixture), 3, and 4 are new triterpenoid-type cycloartanes. Unambiguous (13) C and (1) H NMR assignments for these compounds and the known c...
Article
Solanum chrysotrichum (Solanaceae) synthesizes a family of six antifungal spirostanol saponins designated as SC-1 to SC-6. The production of saponins by wild-type plants is variable depending on the environmental conditions. In order to develop an in vitro system for the sustained production of these saponins, transformed cell suspension cultures o...
Article
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Methanol extracts obtained from eight species belonging to four families of the Lamiales order native to Mexico were investigated for biological action. Cytotoxicity and antioxidant activity of methanol extracts have been investigated. Cytotoxic activity was evaluated by the sulphorhodamine B protein staining assay against KB (nasopharyngeal), HEp-...
Article
This review provides a summary of Mexican medicinal flora in terms of ethnobotanical, pharmacology, and chemistry of natural products related to anticancer activity. Bibliographic investigation was carried out by analyzing recognized books and peer-reviewed papers, consulting worldwide accepted scientific databases from the last five decades. Mexic...
Article
Hexane, chloroform and methanol extracts of different parts of Ipomoea pauciflora were tested for their effects on the survival and development of Fall Armyworm (Spodoptera frugiperda), a Lepidoptera pest. For seven days, neonatal larvae (grown at 27 ± 2°C with a 16: 8 (L: D) h photoperiod) were exposed to different concentrations of crude I. pauci...
Article
2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a single compound, podophyllotoxi...
Article
Ternstroemia pringlei represents one of the most widely employed and commercially exploited medicinal plant in Mexico, used popularly as a tranquilizer and for the treatment of insomnia. To investigate the sedative constituents of the plant through a bio-guided fractionation of extracts derived from calyx and fruits. Crude extracts with different p...
Article
Full-text available
The Mexican species GALPHIMIA GLAUCA (Cav.) Kuntze (Malphigiaceae) synthesises a family of sedative and anxiolytic nor-secofriedelanes, designated as galphimines. These active principles accumulate at low concentration in the aerial parts of plants from wild populations. Transformed calluses and cell suspension cultures of this species were establi...