Maria Shumskaya

Maria Shumskaya
Kean University · CNAHS, Department of Biology

PhD

About

35
Publications
7,211
Reads
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1,084
Citations
Introduction
Maria Shumskaya currently works at the CNAHS, Department of Biology, Kean University. Maria does research in Molecular Biology. Their most recent publication is 'Membrane physical state and stress regulation in Synechocystis : fluidizing alcohols repress fatty acid desaturation'.
Additional affiliations
September 2016 - present
Kean University
Position
  • Professor (Assistant)
January 2014 - September 2016
City University of New York City - Lehman College
Position
  • Lecturer
September 2013 - September 2016
City University of New York City - Lehman College
Position
  • Lecturer
Education
June 2000 - June 2004
Russian Academy of Sciences
Field of study
  • Biochemistry

Publications

Publications (35)
Article
Full-text available
Plants synthesize carotenoids, which are essential for plant development and survival. These metabolites also serve as essential nutrients for human health. The biosynthetic pathway for all plant carotenoids occurs in chloroplasts and other plastids and requires 15-cis-[zeta]-carotene isomerase (Z-ISO). It was not known whether Z-ISO catalyzes isom...
Article
Full-text available
Plant carotenoids have unique physiological roles related to specific plastid suborganellar locations. Carotenoid metabolic engineering could enhance plant adaptation to climate change and improve food security and nutritional value. However, lack of fundamental knowledge on carotenoid pathway localization limits targeted engineering. Phytoene synt...
Article
Full-text available
In previous studies, we characterized five histidine kinases (Hiks) and the cognate response regulators (Rres) that control the expression of approximately 70% of the hyperosmotic stress-inducible genes in the cyanobacterium Synechocystis sp. PCC 6803. In the present study, we screened a gene knock-out library of Rres by RNA slot-blot hybridization...
Article
Full-text available
Saproxylic fungi act as keystone species in forest ecosystems because they colonise and decompose dead wood, facilitating colonisation by later species. Here, we review the importance of intact forest ecosystems to dead wood fungi, as well as trends in their diversity research and challenges in conservation. Saproxylic communities are sensitive to...
Article
Full-text available
DNA structure has been leveraged in a variety of facets that allow scientists to perform a range of assays, including ones for identification of species, establishing evolutionary relationships between taxa, or even identifying individuals. Here, we present a DNA barcoding method as practical, hands-on approach that connects several experimental te...
Article
Full-text available
Using a combination of online tools (discussion board, submission of reports, grading with rubrics) and scaffolded low stakes writing, we designed a comprehensive writing assignment that teaches first and second year undergraduates a basic scientific skill: written communication in the form of a laboratory report. Scaffolding of the assignment into...
Article
Cyanobacteria are model photosynthetic prokaryotic organisms often used in biotechnology to produce biofuels including alcohols. The effect of alcohols on cyanobacterial cell physiology and specifically on membrane fluidity is poorly understood. Previous research on various primary aliphatic alcohols found that alcohols with a short hydrocarbon cha...
Article
Suspended on‐site instruction during the COVID‐19 created an exceptional challenge for teaching hands‐on laboratory classes. We designed an online laboratory activity using computational biology techniques to overcome this issue. This set of online exercises introduces bioinformatics skills into existing curricula in the form of guided tutorials ba...
Article
The main limiting factors for RNA-Seq analysis are quality and quantity of the isolated mRNA. In prokaryotes, the proportion of messenger RNA to total RNA is rather low. Therefore, the main strategy of library preparation for sequencing is mRNA enrichment. Ribosomal and transfer RNAs, both monophosphorylated at the 5′-ends, are the major fractions...
Article
Plant growth is affected by various stresses leading to changes in metabolism. Stress conditions include a variety of biotic and abiotic factors such as pathogens, drought, high and low temperatures and heavy metals. Among multiple physiological responses to stress, there is an adaptive modification in membrane lipid constituents. In particular, th...
Chapter
Carotenoids are essential for survival of all plants, where these colorful pigments and derivatives are biosynthesized, as well as for humans and other species that obtain plant-derived carotenoids in their diets and rely upon them for vitamin biosynthesis or antioxidant actions. The plant carotenoid biosynthetic pathway consists of nuclear encoded...
Article
Full-text available
Systemic analysis of stress-induced transcription in the cyanobacterium Synechocystis sp. strain PCC 6803 identifies a number of genes as being induced in response to most abiotic stressors (heat, osmotic, saline, acid stress, strong light, and ultraviolet radiation). Genes for heat-shock proteins (HSPs) are activated by all these stresses and form...
Article
Full-text available
A new presumably simple consortium of a Leptolyngbya sp. and a Porphyrobacter sp. was isolated from Tolbo Lake in Mongolia. The draft genome sequences of both species are reported. The consortium has been deposited in the Collection of Microalgae and Cyanobacteria of the Institute of Plant Physiology, Moscow, Russia, under the accession number IPPA...
Article
Cyanobacteria are prokaryotic photosynthetic organisms widely used in biotechnology, photosynthesis and abiotic stress research. There are several cyanobacterial strains modified to produce biofuels, but the influence of alcohols on cyanobacterial cell physiology is poorly understood. Here, we conducted a systematic study of the effects of nine pri...
Article
Double mutant ΔkatG/tpx of cyanobacterium Synechocystis sp. strain PCC 6803, defective in anti-oxidative enzymes catalase peroxidase (KatG) and thioredoxin peroxidase (Tpx), is unable to grow in the presence of exogenous H2O2. The ΔkatG/tpx mutant is shown to be extremely sensitive to very low concentrations of H2O2, especially when intensified wit...
Article
Full-text available
A cyanobacterial strain from freshwater Lake Shar-Nuur (Mongolia) was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum In general, strains of Desertifilum maintain their genetic stability, as seen from analysis of 16S rRN...
Article
Full-text available
Carotenoid compounds play essential roles in plants such as protecting the photosynthetic apparatus and in hormone signalling. Coloured carotenoids provide yellow, orange and red colour to plant tissues, as well as offering nutritional benefit to humans and animals. The enzyme phytoene synthase (PSY) catalyses the first committed step of the carote...
Article
Full-text available
Metabolic engineering is a developing field of biological science with a promising future. Biosynthetic pathways can nowadays be altered or introduced into bacterial, plant and animal cells and organisms. Current bioinformaticsdata available of total gene expression, as well as a cell proteome, help with the reconstruction of enzyme pathways for bo...
Patent
Methods are provided for modifying and screening for carotenoid biosynthesis in a plant. The methods are useful for enhancing plant adaptation to climate change and food security, providing increased carotenoid content to a plant, improving stress resistance to climate changes in a plant, and for selecting plants having improved stress resistance t...
Article
Full-text available
Plant carotenoids play essential roles in photosynthesis, photoprotection, and as precursors to apocarotenoids. The plastid-localized carotenoid biosynthetic pathway is mediated by well-defined nucleus-encoded enzymes. However, there is a major gap in understanding the nature of protein interactions and pathway complexes needed to mediate carotenog...
Article
Full-text available
In photosynthetic organisms, carotenoids serve essential roles in photosynthesis and photoprotection. A previous report designated CruP as a secondary lycopene cyclase involved in carotenoid biosynthesis [Maresca J, et al. (2007) Proc Natl Acad Sci USA 104:11784-11789]. However, we found that cruP KO or cruP overexpression plants do not exhibit cor...
Article
Full-text available
Microorganisms respond to hyperosmotic stress via changes in the levels of expression of large numbers of genes. Such responses are essential for acclimation to a new osmotic environment. To identify factors involved in the perception and transduction of signals caused by hyperosmotic stress, we examined the response of Synechocystis sp. PCC 6803,...
Article
Full-text available
The cyanobacterial plasma membrane is an essential cell barrier with functions such as the control of taxis, nutrient uptake and secretion. These functions are carried out by integral membrane proteins, which are difficult to identify using standard proteomic methods. In this study, integral proteins were enriched from purified plasma membranes of...
Article
Full-text available
Oxidative stress caused by reactive oxygen species and, in particular, to hydrogen peroxide (H(2)O(2)) has a major impact on all biological systems, including plants and microorganisms. We investigated the H(2)O(2)-inducible expression of genes in the cyanobacterium Synechocystis sp. PCC 6803 using genome-wide DNA microarrays. Our systematic screen...
Article
Full-text available
Microorganisms respond to hyperosmotic stress via changes in the levels of expression of large numbers of genes. Such responses are essential for acclimation to a new osmotic environment. To identify factors involved in the perception and transduction of signals caused by hyperosmotic stress, we examined the response of Synechocystis sp. PCC 6803,...

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