About
6
Publications
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107
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Introduction
Additional affiliations
April 2019 - September 2020
Position
- PhD Student
Description
- Research focussing on gamma delta (γδ) T cell immunobiology and therapeutic potentials. Working towards a PhD in Prof Ben Willcox's group.
September 2018 - April 2019
Position
- Researcher
Description
- Project focusing on CAR T cell immunotherapy targeting tumour vasculature in vivo. Methods: cell culture, flow cytometry, molecular cloning, in vitro T cell assays (ELISA), in vivo studies of CAR T cell efficacy using mouse tumour models.
October 2017 - August 2018
Position
- Research Student (Cancer Immunology)
Description
- Studied the role of gamma-delta T cells and NK cells in Burkitt lymphoma. Methods: multi-parametric flow cytometry, immune cell purification, cell culture, qPCR, intracellular staining of short-term cytokine assays.
Education
October 2019 - January 2023
September 2017 - September 2018
September 2013 - July 2016
Publications
Publications (6)
The phosphoantigen (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP) is an established activator of Vγ9/Vδ2 T cells and stimulates downstream effector functions including cytotoxicity and cytokine production. In order to improve its drug-like properties, we herein report the design, synthesis, serum stability, in vitro metabolism, and biologi...
gd T cells are generally considered innate-like lymphocytes, however, an ‘‘adaptive-like’’ gd compartment has now emerged. To understand transcriptional regulation of adaptive gd T cell immunobiology, we combined single-cell transcriptomics, T cell receptor (TCR)-clonotype assignment, ATAC-seq, and immunophenotyping. We show that adult Vd1+ T cells...
Aryloxy triester phosphoramidate prodrugs of the monophosphate derivatives of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) were synthesized as lipophilic derivatives that can improve cell uptake. Despite the structural similarity of IPP and DMAPP, it was noted that their phosphoramidate prodrugs exhibited distinct stabili...
Vγ9/Vδ2 T-cells are activated by pyrophosphate-containing small molecules known as phosphoantigens (PAgs). The pres-ence of the pyrophosphate group in these PAgs has limited their drug-like properties due to its instability and polar nature. In this work, we report a novel and short Olefin Grubbs metathesis-mediated synthesis of methylene and diflu...
Bacterial proteins with MCE domains were first described as being important for Mammalian Cell Entry. More recent evidence suggests they are components of lipid ABC transporters. In Escherichia coli, the single-domain protein MlaD is known to be part of an inner membrane transporter that is important for maintenance of outer membrane lipid asymmetr...
Bacterial proteins with MCE domains were first described as being important for M ammalian C ell E ntry. More recent evidence suggests they are components of lipid ABC transporters. In Escherichia coli , the single-domain protein MlaD is known to be part of an inner membrane transporter that is important for maintenance of outer membrane lipid asym...
Questions
Questions (3)
I've recently done a Europium-based cytotoxicity assay where I am interested in the level of killing of some target haematologic cell lines when co-cultured with effector CAR+ T cells. I have done this type of assay before, but this time I've encountered a problem of the value for spontaneous release (ie. target cells without effectors) being higher than the value for experimental release (ie. target cells with effector cells), which results in a negative number when I try to work out % specific lysis/release with the following formula:
% specific release = [(experimental release - spontaneous release)/(maximum release - spontaneous release)] x 100
The assay seems to have worked as the % specific release with CAR-negative effectors is lower than with CAR-positive effectors (as I was expecting), but I am not sure if I can say it worked because all the values are negative. I did the co-culture for 40 minutes (as I had done previously in a slightly different setting), and for 2 hours, which I understand is the recommended time for this particular assay.
Does anyone know why the spontaneous release can be higher than the experimental release?
Thank you very much in advance.
I would like to troubleshoot the transfection stage of my lentiviral transduction protocol. In short, I have 4 lentiviral plasmids, one of which encodes GFP. I tried transfecting the HEK293T cells, harvesting the lentivirus and transducing the Jurkat cells afterwards (as per established protocol) but I had no GFP detected by flow on the Jurkats. Before doing any more transductions, I would like to check whether my transfection is actually working. I’d like to do this by transfecting the 293T cells with the same plasmids again and running the cells on flow cytometer to check for GFP. Can I actually do that though if the cells could potentially be producing the lentivirus post-transfection? Would fixing the cells before running on flow stop the lentiviral production? I know that you can use flow to detect GFP post-transfection but is it safe to do this if I am dealing with lentivirus production ?
Thank you very much in advance for your help.
I keep seeing these two subset names used interchangeably when referring to what looks like the same subset of γδ T cells in literature. I see more of Vγ9Vδ2 but is it the same as Vγ2Vδ2?
Also, on this note, if this refers to some kind of different nomenclature type, does anybody have a link to a paper or an article where these nomenclatures are explained? I don't mean which γδ's are tissue/blood-associated, just the way to name the subsets.
Thank you in advance.
