Maria Concepción Pilar-Izquierdo

Universidad de Burgos | UBU · Department of Biotechnology and Food Science

In this study, the use of spray-drying technology for encapsulating Flavourzyme® (protease-peptidase complex) was evaluated to overcome the limitations (low encapsulation efficiency and no large-scale production) of other encapsulation processes. To the best of our knowledge, spray drying has not been applied previously for the immobilization of th...

In this work, barley seed coating with urease and phosphatase, free and immobilized in humic acids, was studied as a tool to enhance the use efficiency of N and P by plants. The seeds were encapsulated in gels of Ca-alginate by a method that allows gelation of the polymer, containing enzymes, from the surface of seeds outward. The coating process d...

The treatment of must with glucose oxidase (GOX) is a biotechnological alternative to the production of reduced-alcohol wines. Nevertheless, the low pH of must is a limiting factor for the enzyme's activity. This study reports on a simple immobilization method that uses enzyme encapsulation to overcome that limiting factor. The encapsulation of GOX...

Optimization of naringinase immobilization process on PVA-PEG cryogels

BACKGROUND Most of the N fertilizers added to the soil are not efficiently used by plants and are lost to the atmosphere or leached from the soil, causing environmental pollution and increasing cost. The barley seed encapsulation in calcium alginate gels containing free or immobilized urease was investigated to enhance plant utilization of soil N.R...

A large portion of the soluble P fertilizers introduced into soil reacts with soil components forming insoluble P products, which are not efficiently taken up by plants. Barley seed (Hordeum vulgare L. 'Volley') encapsulation in Ca-alginate gels containing phosphatase free or immobilized with soil humates (humate-phosphatase complexes, HPC) was inv...

Coating barley seeds with free and immobilized alkaline phosphatase was investigated as a potential means to enhance plant utilization of accumulated soil phosphorus (P). Two coating techniques were studied: film-coating and pelleting. The highest phosphatase activity retention in the coating layer, ranging from 0·48 to 0·67, was observed when seed...

This study was designed to evaluate and compare antioxidant capacity and radical scavenging activity of naringin and its aglycone by different in vitro assays. The effects of flavanones on lipid peroxidation, glutathione (GSH) oxidation and DNA cleavage were also assessed. The results showed that naringenin exhibited higher antioxidant capacity and...

An alkaline phosphatase (EC 3.1.3.1) from Escherichia coli ATCC27257 was immobilized by copolymerization with resorcinol. The phosphatase-polyresorcinol complex synthesized retained about 74% of the original enzymatic activity. The pH and temperature profile of the immobilized and free enzyme revealed a similar behavior. Kinetic parameters were det...

Neutrase, a commercial preparation of Bacillus subtilis , was covalently immobilized on alginate-glutaraldehyde beads. Immobilization conditions and characterization of the immobilized enzyme were investigated. Central composite design and response surface methods were employed to evaluate the effects of immobilization parameters, such as glutarald...

Alkaline phosphatase (EC 3.1.3.1) extracted from Escherichia coli ATCC27257 was immobilised by co-flocculation with soil humates in the presence of Ca2+. The effects of time, temperature, pH and concentration of enzyme and support on immobilisation were studied. Between 58 and 92% of the added phosphatase was strongly bound to the humates, dependin...