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Maria Mercedes Panizo

Maria Mercedes Panizo

MSc in Mycology; MSc in Quality Management Systems; BSc in Bioanalysis
Head of Examina Clinical Laboratory

About

62
Publications
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September 1998 - present
Instituto Nacional de Higiene "Rafael Rangel"
Position
  • Microbiologist

Publications

Publications (62)
Article
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Purpose of Review In this review, we report on the state of knowledge about human Q fever in Brazil and on the Guiana Shield, an Amazonian region located in northeastern South America. There is a contrast between French Guiana, where the incidence of this disease is the highest in the world, and other countries where this disease is practically non...
Article
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RESUMEN: El objetivo de este estudio fue diagnosticar la conformidad del sistema de gestión de calidad (SGC) de los laboratorios de micología clínica con los requisitos de la norma ISO 9001:2015, para conocer su situación actual frente a la aplicación de esta norma. Se planteó un diseño de campo transversal con enfoque cualitativo, con una muestra...
Article
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Resumen: A propósito de la pandemia causada por el nuevo coronavirus SARS-CoV-2 es pertinente hacer una revisión actualizada sobre la bioseguridad y buenas prácticas de trabajo para laboratorios de microbiología. La definición de bioseguridad comprende los principios, técnicas y prácticas de contención que se implementan para prevenir la exposición...
Chapter
The objective of this article is to review the elements that make up the diagnosis of fungal diseases by conventional and culture independent microbiological methods. The first one includes direct microscopic examination of clinical samples, using wet mounts and stains, and culture in artificial media. Important considerations to take into account...
Article
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Este estudio se enfocó en determinar la capacidad de formación de biopelículas por Klebsiella pneumoniae aislada en heces de pacientes con Trastorno del Espectro Autista (TEA). Se evaluaron 38 pacientes entre 1-18 años de edad. Se recolectaron datos epidemiológicos, disbiosis previa e inicial y tratamiento (antimicrobianos, probióticos y dieta). El...
Article
Pneumocystis jirovecii pneumonia (PCP) is one of the most frequent opportunistic infections in immunocompromised patients. The objective of this study was to know the P. jirovecii epidemiology in Venezuelan patients with Human Immunodeficiency Virus (HIV) infection and suspected pneumonia, through passive surveillance at a national reference labora...
Article
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Purpose of the Review The aim of this work is to contribute to the knowledge of the epidemiology of pneumocystosis or Pneumocystis jirovecii pneumonia (PCP) in Venezuela, by an updated review of the information currently available. Recent Findings The PCP epidemiology in Venezuela is not formally known. There is no national surveillance program fo...
Article
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Purpose of the Review The aim of this work is to contribute to the knowledge of diagnosis, burden, and mortality of pneumocystosis or Pneumocystis jirovecii pneumonia (PCP) in Venezuela. Recent Findings Historically, PCP diagnosis has been clinical and histopathological, but diagnosis based on PCP clinical suspicion, without confirmation of labora...
Article
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Resumen Una de las principales consecuencias del desarrollo de biopelículas es la resistencia a los antifúngicos. Se determinó la formación de biopelículas por Candida parapsilosis sensu stricto aisladas de sangre y su perfil de susceptibilidad. Se evaluaron 30 cepas de C. parapsilosis sensu stricto de la Red de Candidemias del Dpto. de Micología d...
Article
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RESUMEN: La incidencia de candidemia en las unidades de cuidados intensivos neonatales ha aumentado considerablemente en la última década, debido al aumento en la supervivencia de los recién nacidos de bajo peso y a los cambios en los procedimientos diagnósticos y terapéuticos. Se diseñó un estudio descriptivo, retrospectivo de corte transversal, c...
Article
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Resumen: La necesidad de conocer sobre el manejo del conocimiento en las organizaciones ha impulsado el estudio de la Gestión del Conocimiento (GC) en beneficio de las mismas. El objetivo de esta investigación fue caracterizar la GC en organizaciones orientadas a la producción de bienes y servicios. Se realizó una investigación de tipo descriptiva...
Article
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Introduction Disseminated histoplasmosis, a disease that often resembles and is mistaken for tuberculosis, is a major cause of death in patients with advanced HIV disease. Histoplasma antigen detection tests are an important addition to the diagnostic arsenal for patients with advanced HIV disease and should be considered for inclusion on the World...
Article
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Recibido 2 de agosto de 2017; aceptado 26 de septiembre de 2017 Resumen: Cunninghamella bertholletiae es un patógeno oportunista inusual, que pertenece al orden de los Mucorales y causa infección en pacientes inmunosuprimidos, particularmente con enfermedades hematológicas malignas. Se reporta un caso de mucormicosis rinosinusal por C. bertholletia...
Article
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Recibido 2 de agosto de 2017; aceptado 26 de septiembre de 2017 Resumen: Cunninghamella bertholletiae es un patógeno oportunista inusual, que pertenece al orden de los Mucorales y causa infección en pacientes inmunosuprimidos, particularmente con enfermedades hematológicas malignas. Se reporta un caso de mucormicosis rinosinusal por C. bertholletia...
Article
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Cryptococcosis is a sub-acute or chronic mycosis caused by opportunistic yeasts of the Cryptococcus genus, mainly by the C. neoformans and C.gattii species complex. This disease tends to position itself as one of the first opportunistic conditions associated with AIDS, with a high morbidity in these patients, who frequently develop meningoencephali...
Article
Full-text available
Cryptococcosis is a sub-acute or chronic mycosis caused by opportunistic yeasts of the Cryptococcus genus, mainly by the C. neoformans and C.gattii species complex. This disease tends to position itself as one of the first opportunistic conditions associated with AIDS, with a high morbidity in these patients, who frequently develop meningoencephali...
Article
Full-text available
Purpose of Review The purpose of this review is to contribute to the knowledge about the existence of Candida auris as an emerging pathogenic fungus, multi-resistant to antifungal, and causing health care-associated infections (HCAI). Recent Findings C. auris emerges as yeast with clonal transmission resistance to three families of commonly used a...
Article
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Purpose of Review The purpose of this review is to provide a current view of the importance of the determination and use of epidemiological cutoff values (ECVs) for Cryptococcus neoformans, since there are no clinical breakpoints (CBPs). Recent Findings ECVs have been proposed for some antifungal agents and C. neoformans, using standardized method...
Article
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Clinical and Laboratory Standards Institute (CLSI) conditions for testing the susceptibilities of pathogenic Sporothrix species to antifungal agents are based on a collaborative study that evaluated five clinically relevant isolates of Sporothrix schenckii sensu lato and some antifungal agents. With the advent of molecular identification, there are...
Article
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Background: Candida parapsilosis is a species complex consisting of Candida parapsilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis. Studies worldwide have described its epidemiology and susceptibility to antifungal agents. Aims: The aims of this study were to carry out the molecular identification of blood isolates belonging...
Article
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Resumen: La histoplasmosis es una enfermedad causada por Histoplasma capsulatum, que afecta principalmente a pacientes inmunosuprimidos, especialmente con SIDA. El objetivo de este trabajo fue realizar una comparación entre el cultivo y la reacción en cadena de la polimerasa (PCR) anidada en 35 muestras de médula ósea, provenientes de pacientes con...
Article
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Abstract: Histoplasmosis is a disease produced by Histoplasma capsulatum which mainly affects immunosuppressed patients, especially those with AIDS. The aim of this study was to compare culture and nested polymerase chain reaction (nPCR) diagnosis in 35 bone marrow specimens, from patients with AIDS and presumptive clinical diagnosis of disseminate...
Article
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Recibido 24 de julio de 2017; aceptado 29 de septiembre de 2017 Resumen: El objetivo de esta investigación fue evaluar la utilidad del medio Litmus Milk ® para la diferenciación de los complejos de especies Trichophyton rubrum y Trichophyton mentagrophytes. Se emplearon 52 aislamientos del género Trichophyton, identificados a nivel de género y espe...
Article
Full-text available
Recibido 24 de julio de 2017; aceptado 29 de septiembre de 2017 Resumen: El objetivo de esta investigación fue evaluar la utilidad del medio Litmus Milk ® para la diferenciación de los complejos de especies Trichophyton rubrum y Trichophyton mentagrophytes. Se emplearon 52 aislamientos del género Trichophyton, identificados a nivel de género y espe...
Article
Full-text available
The superficial mycoses are very common infectious diseases and therefore are a frequent reason for medical consultation. The aim of this study was to determine the diagnostic frequency of superficial mycoses in the Mycology Department of the Instituto Nacional de Higiene "Rafael Rangel" during 14 years (2001-2014). A retrospective cross-sectional...
Article
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La Micoteca del Instituto Nacional de Higiene “Rafael Rangel” (INHRR) fue creada en el año 1955 y es la colección de hongos microscópicos autóctonos más grande y representativa del país. Cuenta con 2.500 cepas pertenecientes a 77 géneros y 165 especies de hongos y actinomicetos, de importancia médica, epidemiológica, industrial e histórica, preserv...
Article
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Resumen. El objetivo de este trabajo fue comparar la identificación de le-vaduras de interés clínico por los métodos automatizados Vitek YBC® y Mi-croscan Walk Away RYID® con los métodos fenotípicos convencionales. Se utilizaron 193 aislamientos de levaduras provenientes de muestras clínicas y cinco cepas controles. Todas las levaduras fueron ident...
Article
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Resumen: El Complejo Fusarium solani (CFS) se encuentra distribuido en la naturaleza, causando un amplio espectro de infecciones en los humanos, desde superficiales, como la queratitis, hasta infecciones fúngicas invasoras, caracterizándose por su resistencia a los antimicóticos. El objetivo de esta investigación fue determinar la susceptibilidad i...
Article
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Pneumocystis jirovecii pneumonia (PcP) is a well-recognized major opportunistic infection in HIV-infected patients. During the 1980s, the HIV pandemic turned PcP into a major worldwide medical and public health problem. With the introduction of Pneumocystis chemoprophylaxis and the development of highly active antiretroviral therapy (ART) for the t...
Article
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Resumen: En el presente estudio fueron evaluadas 300 cepas de actinomicetos (140 de origen clínico y 160 del suelo). La producción de sustancias antimicrobianas del total de las cepas de actinomicetos fue evaluada utilizando los siguientes caldos: Bennett (M1), (M2), y el caldo levadura extracto de malta-dextrosa YMD (M3). Se ajustaron las condicio...
Article
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Resumen: El objetivo de este trabajo fue validar la preparación del inóculo por densitometría para las pruebas de susceptibilidad a los antifúngicos en especies del género Fusarium. Se emplearon 15 aislamientos clínicos de Fusarium spp. para preparar los inóculos por espectrofotometría y contaje de unidades formadoras de colonias en cámara de Neuba...
Article
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Resumen Weimberger José, Chimá Leydis, Navas Trina, Panizo María. Tiña incógnita por el complejo Trichophytum rubrum: presentación de un caso en paciente usuario de esteroides de forma crónica. Salus Militiae 2013;38 (1 y 2): 12/4/2015 TIÑA INCÓGNITA POR EL COMPLEJO TRICHOPHYTUM RUBRUM: PRESENTACIÓN DE UN CASO EN PACIENTE USUARIO DE ESTEROIDE… http...
Article
Les espèces du genre Nocardia sont des bactéries filamenteuses, aérobies strictes et à coloration de Gram positive. Le système d’identification traditionnel de ces espèces est basé sur l’étude des caractéristiques biochimiques et chimiotaxonomiques. Toutefois, les nouvelles méthodes basées sur l’étude de l’ARN ribosomal 16S ont conduit à une augmen...
Article
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Antecedentes: la transmisión interhumana de Pneumocystis jirovecii y su relación con la colonización es controversial. Objetivos: detectar por pruebas moleculares e Inmunofluorescencia Directa (IFD) la presencia del hongo en los contactos de pacientes con diagnóstico confirmado de neumocistosis pulmonar. Métodos: Se realizó un estudio prospectivo y...
Article
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The patient with malignancy disease has a high risk to develop respiratory infections for Pneumocystis jirovecii pneumonia. Investigations about pneumocystosis in oncological patients in Venezuela are scarce. The objective of this work was to detect Pneumocystis jirovecii in oncological patients by the method of direct immunofluorescence technique....
Article
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The in vitro susceptibility of 62 isolates of Sporothrix schenckii in its mycelial form, from Latin-American countries (Peru, Venezuela, Brazil and Uruguay) and Spain, to amphotericin B (AB), itraconazole (IZ), posaconazole (PZ) and terbinafine (TB) was determined by measuring the MICs and minimum fungicidal concentrations (MFCs) using a standardiz...
Article
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The aim of this study was to determine in vitro susceptibility profiles of Venezuelan strains of Candida spp. to four antifungal agents. One hundred and forty five (145) isolates were recovered during a 1-year period (June 2006 to June 2007) from clinical specimens of patients with severe Candida spp. infections in 15 hospitals. In vitro susceptibi...
Article
The objective of this work was to investigate the epidemiology of pneumocystosis in Venezuelan patients utilizing a retrospective study during a six year period. One hundred and twenty nine clinical samples collected from patients with AIDS, cancer and non-AIDS-non-cancer low respiratory tract infection patients were processed by direct immunofluor...
Article
Full-text available
The objective of this work was to investigate the epidemiology of pneumocystosis in Venezuelan patients utilizing a retrospective study during a six year period. One hundred and twenty nine clinical samples collected from patients with AIDS, cancer and non-AIDS-non-cancer low respiratory tract infection patients were processed by direct immunofluor...
Article
El objetivo de este estudio fue conocer la frecuencia y la sensibilidad a los antifúngicos de aislamientos clínicos de Candida provenientes de pacientes con candidiasis en seis centros de salud del área metropolitana de Caracas, Venezuela. Se revisaron retrospectivamente los informes de laboratorio desde enero de 2003 hasta agosto de 2005. La ident...
Article
The aim of this study was to investigate the frequency and antifungal susceptibility of Candida clinical isolations coming from patients with candidiasis in six health care centers of Caracas, Venezuela metropolitan area. The laboratory reports were retrospectively revised from January 2003 through August 2005. The isolated yeasts identification wa...
Article
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Background: Person to person transmission of Pneumocystis jirovecii (Pj.) has been confirmed. This is important to recognize it´s presence in contacts. Objectives: The following were assessed: frequency of infection by Pj in patients with acute respiratory infections, probability of positive Pj. in contacts and to apply clinical epidemiology en the...
Article
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Inmunodiagnóstico; micosis sistémicas; histoplasmosis; paracoccidioidomicosis; coccidioidomicosis; inmunodifusión; criptococosis; inmunodifusión; aglutinación con látex
Article
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The objectives of this work were to determine and to compare the phospholipase and protease activity of Candida spp. strains, and to correlate the enzymatic activity with the place of yeasts isolation. 95 Candida spp. strains isolated of different clinical samples were used. Phospholipase activity was performed according to Polak, using two egg yol...
Article
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To maintain and to conserve a fungal culture collection is a task that demands constant dedication and surveillance. It requires to know morphological, physiological and biochemical characteristics of fungi, as well as their requirements for preservation methods. The main objective of this work was to evaluate viability, purity and morphological st...
Article
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Historical details of the Medical Mycology development in Venezuela are checked and the creation of the Medical Mycology Post Grade Course, as an answer to the real country needs in the preparation of a relay generation, that will occupy of the diagnosis and prevention of the mycotic diseases. The objectives of the course, the institutes and the na...
Article
Fungal infections produced by Candida species, specially by Candida albicans, are important complications among immunocompromised patients. Morbility and mortality rates of these infections have increased considerably during the past two decades. Frequently, mucosal candidiasis (oral, gastrointestinal, and vaginal) is the first sign of impaired imm...
Article
The purpose of this review is about possible therapeutic applications of immunomodulators during the treatment of systemic candidosis, as some as the mechanisms of their immunopathology.
Article
The purpose of this article is to make a review of the Candida albicans adherence property to the epithelial cells, which has been considered an important virulence factor in the candidal pathogenesis at mucosal surfaces.
Article
Histoplasmosis is a granulomatous systemic disease of worldwide distribution, caused by the dimorphic fungus Histoplasma capsulatum. The most important endemic areas of this mycosis surround the American continent, because of its propitious climatic conditions for the development of this fungus. The illness has close relationship with professional...
Article
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La resistencia de las levaduras a los agentes antifúngicos fue por muchos años un problema de poca importancia, pero recientemente se ha reportado un incremento en la resistencia a estas drogas, especialmente entre las especies de Candida no-albicans, las cuales, a su vez, aparecen con mayor frecuencia en pacientes con Candidosis Vulvovaginal Recur...
Article
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Background: Infection by Pneumocystis jirovecii has usually been considered present only in patients with AIDS complex. Objectives: To assess the following vari- ables: frequency of infection by Pneumocystis jirovecii in a sample with risk factors for the disease, their comorbid conditions and to document infection by Pneumocystis jirovecii by immu...

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Projects (3)
Project
To know the epidemiology of Pneumocystosis in Venezuelan patients Impact of Pneumocystosis in HIV/AIDS patients, cancer patients and non AIDS/non cancer patients.
Project
Cryptococcosis is a fungal infection of subacute or chronic course caused by an opportunistic yeast from genus Cryptococcus, mainly by species of the C. neoformans (CNC) and C. gattii (CGC) complexes. Cryptococcosis tends to position itself as one of the first opportunistic infections associated with AIDS, with a high rate of morbidity in these patients who often develop meningoencephalitis. In Venezuela, information on the serotypes, antifungal susceptibility, and virulence of circulating strains is scarce. The aim of this study was to perform the molecular characterization of Venezuelan isolates from the C. neoformans and C. gattii complexes by PCR-RFLP (Amplification of URA5 gene and subsequent digestion with the restriction enzymes Sau96I and HhaI). This study will provides epidemiological information on circulating molecular types and will allows deeper understanding of this opportunistic fungal infection in Venezuela