Maria Carmen Hernandez‐Cruz

Universidad de Las Palmas de Gran Canaria | ULPGC · Department of Biology

Optimizing the growth and survival of marine fish larvae, while reducing the cost of production, is important for the development of viable hatcheries on an industrial scale. This study was aimed to determine whether Artemia sp. use could be minimized while maintaining high growth and survival, and low skeleton anomalies occurrence in greater amber...

Systematic granulomatosis is a chronic disease that affects the majority of farmed meagre (A. regius). Nutritional imbalances and overcrowding can increase the risk to suffer oxidative stress, and consequently, impact the incidence of granulomatosis. In order to evaluate this, juvenile meagre were fed five isolipidic (16.7%) and isoproteic (49.6%)...

Systematic granulomatosis is a chronic disease that affects the majority of farmed meagre (A. regius). Nutritional imbalances and overcrowding can increase the risk to suffer oxidative stress, and consequently, impact the incidence of granulomatosis. In order to evaluate this, juvenile meagre were fed five isolipidic (16.7%) and isoproteic (49.6%)...

Abstract In previous studies, combined inclusion of Zn, Mn and Se in early weaning diets improved larval growth, but suggested a potential toxicity by one of these elements. The present study aimed to determine the effect of the single inclusion of Zn, Mn, Se or Cu, their combination (Control+) or their absence (Control−) on larval diets. At the en...

The present study investigated the effects of nutritional programming through parental feeding on offspring performance and expression of selected genes related to stress resistance in a marine teleost. Gilthead seabream broodstock were fed diets containing various fish oil (FO)/vegetable oil ratios to determine their effects on offspring performan...

Systemic granulomatosis has already been reported in meagre larvae with an adequate feeding protocol and enrichment media preventing its appearance in the first weeks of life. Afterwards, the control of this disease could be prevented through nutritional components of the inert food, being the antioxidants the key to success. For this reason, in th...

To characterise the progression of lipid digestion capacity in gilthead seabream across life cycle, the activities of bile salt-activated lipase (BAL) and phospholipase A2 (PLA2) were determined in the digestive tracts of cultured gilthead seabream from first feeding to marketable size (49 μg to 300 g). Four trials were undertaken with gilthead sea...

Introducción: En los últimos años ha incrementado considerablemente el interés en varios aspectos del metabolismo lipídico en las larvas de peces marinos y su relación con el crecimiento. La utilización de los lípidos dietéticos y los requerimientos nutricionales van a verse directamente afectados por diversos cambios morfológicos y fisiológicos qu...

Systemic granulomatosis is the most frequent disease in juvenile and adult meagre, but studies regarding the first appearance of granulomas in larvae do not exist. In order to evaluate this, meagre larvae were fed four different feeding regimes as follows: RS and RO (rotifer enriched with Easy DHA Selco or Ori‐Green from 3 to 30 dph respectively),...

Abstract To characterise the progression of lipid digestion capacity in gilthead seabream across life cycle, the activities of bile salt-activated lipase (BAL) and phospholipase A2 (PLA2) were determined in the digestive tracts of cultured gilthead seabream from first feeding to marketable size (49 μg to 300 g). Four trials were undertaken with gil...

Elevated mortality during early stages and high occurrence of skeletal anomalies are major concerns in Greater amberjack (Seriola dumerili) juvenile's production. Identification of nutritional requirements during live prey feeding stages, particularly those related with n-3 HUFA would help to formulate specific diets for this species improving larv...

Systemic granulomatosis is a growing disease with a high morbidity, which affects to the majority of farmed meagre (Argyrosomus regius). The impossibility of isolating any infectious agents has hypothesized a nutritional origin of the disease. In order to try to elucidate the nutritional origin of granulomas, juvenile meagre were fed for 15 weeks w...

A well-balanced diet adapted to fulfil the specific nutritional requirements of greater amberjack (Seriola dumerili) would contribute to optimize reproduction and spawning quality. The main objective of the present study was to examine the effects of supplemental histidine, taurine and protein in broodstock diets, on egg quality of greater amberjac...

The negative effects of oxidative damage on larval development, growth and survival are among the factors markedly affecting marine fish larvae production success. Where as α-tocopherol (vitE) and L-ascorbic acid (vitC) are strong antioxidants, the potential antioxidant role of taurine (Tau) in fish diets and its relation with other antioxidant nutrien...

Análisis sensorial de cinco especies de espáridos (besugo, dentón, hurta, pargo y sargo picudo) en ocho localidades costeras españolas. Resumen. El objetivo del presente estudio ha sido conocer la valoración de las nuevas especies de espáridos por el consumidor. Para ello, se realizó una prueba de aceptación sobre 5 especies de espáridos: besugo, d...

Six experimental groups and two control groups were formed. From the control group one of them was not injected at all, and the other was injected with a saline solution. The six experimental groups were induced with GnRHa injections (Sigma-Aldrich Co. St. Louis, Missouri,USA) with doses of 1, 5, 10, 15, 20 and 25 μg.kg-1, respectively. No signific...

Five experimental groups were formed and were induced with GnRHa injections with a dose of 15μg.kg-1, every 3, 4, 5, 6 and 7 days, along the reproductive period. No significant differences were found in any of the sexual maturation parameters studied: oocytes measurement, density, sperm motility and activity. The highest spawning parameters were ob...

Six experimental groups and two control groups were formed. From the control group one of them was not injected at all, and the other was injected with a saline solution. The six experimental groups were induced with GnRHa injections (Sigma-Aldrich Co. St. Louis, Missouri,USA) with doses of 1, 5, 10, 15, 20 and 25 μg.kg-1, respectively. No signific...

This study was focus on the determination of optimum DHA levels in microdiets during P. dentex larval rearing. A total of15 tanks were settled with an initial population of 150 larvae per tank at 19 dph which were reared until 45 dph. Present results showed that diets with a DHA content of around 4% promote better growth and survival rate of P. den...

Two females bearing oocytes larger than 500μ and four fluent males were selected for induction with successive injections of 20 μg.kg-1 GnRHa (Sigma-Aldrich Co. St. Louis, Missouri, USA). Broodstock kept in 10 m3 tanks, under natural temperature and photoperiod conditions in the Canary Islands. A total of twenty-two spawns were obtained with fiftee...

Two females with oocytes bigger than 500μ, and four fluent males, originally captured from the wild and acclimated to the broodstock of the GIA were used for this study. Fish were acclimated in tanks of 10 m3, and they were injected three times per month (June till November) with 20μg.kg-1 of GnRha. A total of 33 spawns with 15 injections were obta...

El objetivo del presente estudio fue la obtención de puestas viables, mediante inducción con GnRHa, de reproductores, capturados en el medio natural y mantenidos en condiciones de cultivo de S. dumerili, y de la determinación de su producción

The aim of this work was to test the effect of different DHA formulated levels in experimental microdiets fed to gilthead sea bream (Sparus aurata) larvae on their biological performance in terms of survival, growth and incidence of skeletal deformities.

Striped Jack Pseudocaranx dentex (Bloch & Schneider, 1801) as other carangid fish such as some Seriola species has a worldwide distribution. This species, presents a moderate fast growth (Gardner y Carter, 1999; Roo et al., 2005) being considered a potential candidate for aquaculture diversification due to its flesh quality and high market prices....

Two females bearing oocytes larger than 500μ and four fluent males were selected for injection of 20 μg.kg-1 GnRHa (Sigma-Aldrich Co. St. Louis, Missouri, USA). Broodstock, kept in 10 m3 tanks, under natural temperature and photoperiod conditions in the Canary Islands produced a high spawn quality: 92.28% fertilized eggs, 55.48% viable eggs, 77.76%...

The aim of present work is to report the advances in the last three years of studies, such as the obtention of viable spawns and first larval rearing trials applying both intensive and semi-intensive techniques for larval rearing of this specie.

Two females with oocytes bigger than 500μ, and four fluent males, were acclimated in a tank of 10 m3, and were injected three times per month (June till November) with 20μg.kg-1 of GnRha. The number of eggs obtained ranged from a minimum of 1,680,500 in June and a máximum of 6,699,000 in September, and the total was of 20,189,700. A positive relati...

Determinar el efecto de tres distintos tratamientos hormonales (GnRHa y GCH inyectadas y GnRHa mplantada), sobre las roducciones de reproductores de corvina (Argyrosomus regius) nacidos en cautividad.

The aim of the present work is to study the effect of stocking egg densities on larval performance in terms of growth, survival and skeletal deformities.

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Systemic granulomatosis is a growing disease with a high morbidity, which affects to the majority of farmed meagre (Argyrosomus regius). The impossibility of isolating any infectious agents has hypothesized a nutritional origin of the disease. In order to try to elucidate the nutritional origin of granulomas, juvenile meagre were fed for 15 weeks w...

Five isoproteic (54.8%) and isolipidic (24.1%) microdiets, which varied in their docosahexaenoic acid (DHA) content (0.25%, 0.75%, 1.64%, 1.99% and 3.17%; dw), were manufactured to determine its effects on longfin yellowtail Seriola rivoliana larvae in terms of fish biological performance, whole body fatty acid profile and incidence of skeletal ano...

Reproducción en Seriola

Reproducción Seriola

Nutrition during periconception and early development can modulate metabolic routes to prepare the offspring for adverse conditions through a process known as nutritional programming. In gilthead sea bream, replacement of fish oil (FO) with linseed oil (LO) in broodstock diets improves growth in the 4-month-old offspring challenged with low-FO and...

Recent evidence suggests that environmental factors experienced by the parents can have long-lasting effects in the offspring or in the later generations. Thus, early environmental signs, such as available nutrients during reproduction, can modulate metabolic routes and offspring phenotype. This metabolic regulation, known as nutritional programmin...

This presentation will review the most recent advances obtained in broodstock and larval rearing management of greater amberjack (Seriola dumerilli) during the last three years at the Aquaculture Research Group (GIA) from the University of Las Palmas de Gran Canaria. A series of studies on hormonal induction strategies, broodstock nutrition, larval...

The culture of Octopus vulgaris is constrained by unsolved problems in paralarvae rearing, mainly associated to the unknown nutritional requirements of this species in early stages. In this article we studied the fatty acid profile (total, neutral, and polar lipid fractions) in wild eggs and wild hatchlings, collected in Gran Canaria (SW) (Spain) w...

Early weaning of marine fish larvae with dry diets delays gut maturation and reduces growth rates. In juvenile and adult forms of several marine fish species, inclusion of dietary mannan oligosaccharides (MOS) improves gut integrity and functionality, but the effects of MOS inclusion in gilthead sea bream (Sparus aurata, L.) larval diets have not b...

Skull, pectoral and pelvic fin bone structures in longfin yellowtail Seriola rivoliana were studied from 3.43 ± 0.15 to 16.20 ± 0.73 mm standard length (SL) specimens. The S. rivoliana skull started to mineralize with the appearance of the parasphenoid and maxillary by 3.43 ± 0.15 mm SL at the neurocranium and jaw regions, respectively. The first p...

Seven experimental isonitrogenous (50%) and isolipidic (16%) diets with different levels of α-tocopherol acetate (16, 100, 190, 285, 430, 880 and 1300 mg kg− 1) were tested during 72 days to evaluate growth performance, tissue composition, fillet oxidation and liver histology in meagre juveniles, Argyrosomus regius. Growth performance, feed convers...

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Marine fish larvae are subjected to high oxidative stress levels that oxidize lipids, proteins and DNA. In addition, diets for marine fish larvae also contain high levels of lipids and pro-oxidants. In this sense, auto-oxidaHon of lipids is a potential problem for larval development. There are few studies about the nutritional requirements of Tauri...

Microdiets are being used to partially replace live feed in Gilthead seabream (Sparus aurata) larvae. However information on requirements of micronutrients for this species is scarce. Skeletal anomalies are highly present in seabream culture, and prevalence of severe anomalies ranges between 15-100% in commercial hatcheries. A wide range of factors...

Fish oil (FO) replacement by more sustainable alternative sources such as vegetable oils (VO) has been center of the attention during the last decades in fish nutrition research (Turchini et al., 2009; Torstensen et al., 2010). A number of studies have showed high percentage VO inclusion may cause adverse effects on growth performance and health st...

Despite the importance of trace mineral for the normal development of marine fish larvae, previous studies have demonstrated that the contents of this nutrients in live preys used in larviculture are much lower than those of copepods. Moreover, our previous studies have shown the importance of a premix inclusion of Zn, Mn and Se in early weaning di...

Nutrition during peri-conception and early development can modulate metabolic routes to prepare the offspring for adverse conditions. This phenomena, known as nutritional programming, has been recently deserves more attention in fish production. Our previous studies have demonstrated that replacing fish oil (FO) by linseed oil (LO) in broodstock di...

Levels of the oxidative stress-related minerals selenium (Se), zinc (Zn) and manganese (Mn) that should be supplied in microdiets for marine fish larvae depend on the availability of the molecular form of these minerals. The objective of this study was to determine how effectively Se, Zn and Mn in organic, inorganic and nanoparticle forms promote l...

This study aimed to determine the effects of increasing docosahexaenoic acid (DHA) levels in Sparus aurata diets supplemented with antioxidants vitamins (vitamins C and E). Six isoproteic and isolipidic formulated diets, which varied in the DHA content (38–78 g kg−1 TFA), were fed to 19 dph S. aurata larvae. No significant differences were found in...

Eighteen mature meagre (Argyrosomus regius) broodstock, born in captivity, were divided in 3 experimental groups in ratios of 3 ♀ and 3 ♂ each. One group was inducted by injections of hCG, the other with GnRHa and the last group with implants of GnRHa. In all three treatments all females responded positively to the Hormonal treatment. Statistically...

In the present study, the effect of three different hormonal treatments were tested (GnRHA and hCG injected and GnRHa implanted), in meagre (Argyrosomus regius) broodstock born in captivity, and their production. The highest productions per kg of female and spawn: number of eggs, fertilized eggs, hatched larvae, and larvae 3 days old after hatching...

In this experiment, quality spawns were compared, in mature meagre broodstock (Argyrosomus regius), born in captivity, treated with three hormonal treatments: injection GnRHa and hCG, and implants of GnRHa. The best results in all quality parameters, were obtained in the spawns of the broodstock injected with GnRHa, having 98,7 + 1,4% of fertilized...

For this study, a total of 19 greater amberjack (Seriola dumerili) broodstock were used. Females with oocites bigger than 600µ, and fluent males with a mean weight of 10.72±1.22 kg, fameles, and 10.77±2.33 kg males. The experimental tanks were circular tanks of 40 m3 capacity. One of the tanks were not induced and the other two were with GnRHa, one...

Greater amberjack (Seriola dumerili) broodstock, with a median weight of 10.94±1.21 kg, for females and 11.69±2.35 kg, males, were distributed in two circular tanks of 40 m3. Tank 1, three males and three females were intramuscular injected with GnRHa at a dose of 20 µg.kg-1body weights. The three males and three females of tank 2 were induced with...

Despite the interest of meagre (Argyrosomus regius) as a fast-growing candidate for Mediterranean aquaculture diversification, there is a lack of information on nutrition along larval development. Importance of highly unsaturated fatty acids (HUFA) and the antioxidant vitamins E and vitamin C has not been investigated yet in this species. Six diets...

For this study, a total of 19 grater amberjack (Seriola dumerili) broodstock were used. Females with, oocytes bigger than 600µ, and fluent males with a mean weight of 10.72±1.22 kg, females, and 10.77±2.33 kg males. The experimental tanks were circular tanks of 40 m3 capacity. One of the tanks was not induced and the other two were with GnRHa, one...

In this study, the feasibility of the use of heterotrophic dinoflagellate Crypthecodinium cohnii and phototrophic eustigmataceae Nannochloropsis gaditana as a partial or total substitute for fish oil in weaning diets of gilthead sea bream is evaluated. 20 day old larvae were fed with the following microdiets: a control diet based on fish oil, two d...

The concept of nutritional programming raises the interesting possibility of directing specific metabolic pathways or functions in juvenile fish, for example, to improve the use of substitutes to fishmeal and oil, and hence to promote sustainability in aquaculture. The aim of the study was to determine effects of early nutritional stimuli of gilthe...

The present study aimed to determine the spawning efficacy, egg quality and quantity of captive breed meagre induced with a single gonadotrophin-releasing hormone agonist (GnRHa) injection of 0, 1, 5, 10, 15, 20, 25, 30, 40 or 50 μg kg–1 to determine a recommended optimum dose to induce spawning. The doses 10, 15 and 20 μg kg–1 gave eggs with the h...

Bone and fins development in Seriola rivoliana were studied from cleared and stained specimens from 3 to 33 days after hatching. The vertebral column began to mineralize in the neural arches at 4.40 ± 0.14 mm Standard Length (SL), continued with the haemal arches and centrums following a cranial-caudal direction. Mineralization of the caudal fin st...

To optimize broodstock management for Seriola rivoliana, a survey over 5 years was performed to evaluate the effects of successive hormonal inductions with GnRHa or dietary regime on spawning quality. Running males and females with an oocyte diameter > 500 μm were injected with GnRHa. The spawning quality was compared among consecutive years and di...

Meagre (. Argyrosomus regius) have good potential for aquaculture. Culture characteristics include controlled spawning in captivity, relatively easy larval rearing, fast growth, good feed conversion ratios and no maturation during ongrowing. Whilst characteristics of the final product include good processing yield, low fat content, good taste and f...

Reliable reproduction and sufficient amounts of juveniles obtained in captivity constitute a main bottleneck for mass production of greater amberjack. The aim of the present study was to combine several aspects of broodstock management and hormonal induction, to obtain a large number of high quality spawns. Captured Seriola dumerili sub-adults were...

Dietary omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have a marked effect on fish behavior. There is limited information on the mechanisms involved in this effect and its relation to neuron development and functioning. Deficiency of n-3 LCPUFA reduces fish escape swimming....

Two females bearing oocytes larger than 500μ and four fluent males were selected for injection of 20 μg.kg-1 GnRHa (Sigma-Aldrich Co. St. Louis, Missouri, USA). Broodstock, kept in 10 m3 tanks, under natural temperature and photoperiod conditions in the Canary Islands produced a high spawn quality: 92.28% fertilized eggs, 55.48% viable eggs, 77.76%...

El objetivo del presente estudio fue la obtención de puestas viables mediante inducción con GnRHa, de reproductores, capturados en el medio natural y mantenidos en condiciones de cultivo de S. rivoliana, y de la determinación de la calidad de las mismas.