Maria Fátima Grossi-de-Sá

Maria Fátima Grossi-de-Sá
Brazilian Agricultural Research Corporation (EMBRAPA) | Embrapa · Embrapa Genetic Resources & Biotechnology

Ph.D
Plant Science and Agronomy

About

381
Publications
79,791
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9,063
Citations
Citations since 2016
156 Research Items
4412 Citations
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
Introduction

Publications

Publications (381)
Article
Full-text available
Cotton is the most important crop for fiber production worldwide. However, the cotton boll weevil (CBW) is an insect pest that causes significant economic losses in infested areas. Current control methods are costly, inefficient, and environmentally hazardous. Herein, we generated transgenic cotton lines expressing double-stranded RNA (dsRNA) molec...
Article
Full-text available
Key message The overexpression of the soybean GmEXPA1 gene reduces plant susceptibility to M. incognita by the increase of root lignification. Abstract Plant expansins are enzymes that act in a pH-dependent manner in the plant cell wall loosening and are associated with improved tolerance or resistance to abiotic or biotic stresses. Plant-parasiti...
Article
Full-text available
The root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) a...
Article
Full-text available
The global increase in temperature and associated meteorological disruptions, such as the earlier onset of high temperatures and disruptions in precipitation, are becoming severely limiting factors in crop cultivation. Chickpea, as a cool season crop, is under the direct influence of heat and drought stress that is not only affecting this crop in i...
Article
Full-text available
Main Conclusion The pUceS8.3 is a constitutive gene promoter with potential for ectopic and strong genes overexpression or active biomolecules in plant tissues attacked by pests, including nematode-induced giant cells or galls. Abstract Soybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oi...
Article
Full-text available
Gossypium hirsutum L. represents the best cotton species for fiber production, thus computing the largest cultivated area worldwide. Meloidogyne incognita is a root-knot nematode (RKN) and one of the most important species of Meloidogyne genus, which has a wide host range, including cotton plants. Phytonematode infestations can only be partially co...
Article
Full-text available
The efficiency of RNAi technology in insects varies considerably, particularly in lepidopterans. An important limiting factor of RNAi-mediated gene silencing is the degradation of dsRNA by insect nucleases before cellular uptake. To date, few studies have reported effective gene knockdown in the sugarcane borer Diatraea saccharalis. However, yieldi...
Article
Full-text available
Key Message pGhERF105 and pGhNc-HARBI1 promoters are highly responsive to CBW infestation and exhibit strong activity in vegetative and reproductive tissues, increasing their potential application in GM crop plants for pest control. Abstract The main challenge to cotton (Gossypium hirsutum) crop productivity is the constant attack of several pests...
Article
Full-text available
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture...
Article
Full-text available
Cotton Boll Weevil (CBW, Anthonomus grandis) is a devastating insect-pest affecting cotton (Gossypium spp), using cotton internal floral structures for larval development, making it difficult to control. Little is known about the mechanism underlying CBW-plant interactions leading to infestation or resistance. Here, we investigated the plant molecu...
Poster
Full-text available
First Poster Prize in Seventh International Congress of Nematology 2022 (ICN2022). Category: Nematode management First author: Valdeir Junio Vaz Moreira Supervisor: Maria Fátima Grossi de Sá Co-supervisor: Janice de Almeida Engler Description: This study describes the effect of Minc03328 gene silencing in Meloidogyne incognita via the uptake of...
Article
Full-text available
Malaria is one of the severe infectious diseases that has victimized about half a civilization billion people each year worldwide. The application of long-lasting insecticides is the main strategy to control malaria; however, a surge in antimalarial drug development is also taking a leading role to break off the infections. Although, recurring drug...
Article
Meloidogyne incognita is the most economically important species of the root-knot nematode complex causing damage to several crops worldwide. During parasitism in host plants, M. incognita secretes several effector proteins to suppress the plant immune system, manipulate the plant cell cycle, and promote parasitism. Several effector proteins have b...
Article
Cowpea (Vigna unguiculate L. Walp) is a legume of great economic importance, however it is highly affected by nematodes. The present work aimed to identify proteins and genes involved in nematode resistance by proteomic and transcriptomic analysis. Plants of a genotype resistant (31CE) to root-knot nematode (Meloidogyne spp.) were collected 12 days...
Article
Full-text available
Main conclusion Minc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Abstract Meloidogyne incognita is the most...
Article
Full-text available
Main conclusion: Host-derived suppression of nematode essential genes decreases reproduction of Meloidogyne incog-nita in cotton. Abstract: Root-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the...
Article
Full-text available
Abstract: Promoters are upstream gene regulatory sequences recognized by transcription factors (TFs) involved in controlling transcription initiation and progression. For modern crop improvement, the design of efficient gene constructs relies on promoter efficiency, tissue specificity, and other characteristics that allow the introgression of agron...
Article
Full-text available
The savannah biome occupies about 23% of Brazil's surface area, considered the second largest biome in Brazil. It has about 4,800 endemic species, including the fruiting species Eugenia dysenterica DC. (cagaita tree). Rich in nutrients, this species has economic potential in Brazil, as it has actions directly related to human health. This study car...
Article
Several economically important crops are susceptible to root-knot nematode (RKNs). Meloidogyne incognita and M. javanica are the two most reported species from the RKN complex, causing damage to several crops worldwide. The successful outcome of the Meloidogyne-plant interaction is associated with molecular factors secreted by the nematode to suppr...
Article
Full-text available
Main conclusion The combined Agrobacterium- and biolistic-mediated methods of cotton transformation provide a straightforward and highly efficient protocol for obtaining transgenic cotton. Abstract Cotton (Gossypium spp.) is the most important crop for natural textile fiber production worldwide. Nonetheless, one of the main challenges in cotton pr...
Article
Full-text available
Nematodes and drought are major constraints in tropical agriculture and often occur simultaneously. Plant responses to these stresses are complex and require crosstalk between biotic and abiotic signaling pathways. In this study, we explored the transcriptome data of wild Arachis species subjected to drought (A-metaDEG) and the root-knot nematode M...
Article
Full-text available
NACs are plant-specific transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NAC-SAGs belong to well-characterized regulatory networks, poorly understood...
Article
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Early plants began colonizing earth about 450 million years ago. During the process of coevolution, their metabolic cellular pathways produced a myriad of natural chemicals, many of which remain uncharacterized biologically. Popular preparations containing some of these molecules have been used medicinally for thousands of years. In Brazilian folk...
Article
The Coffea arabica HB12 gene (CaHB12), which encodes a transcription factor belonging to the HD-Zip I subfamily, is upregulated under drought, and its constitutive overexpression (35S:CaHB12OX) improves the Arabidopsis thaliana tolerance to drought and salinity stresses. Herein, we generated transgenic cotton events constitutively overexpressing th...
Article
Full-text available
The biogenesis of root-knot nematode (Meloidogyne spp.)-induced galls requires the hyperactivation of the cell cycle with controlled balance of mitotic and endocycle programs to keep its homeostasis. To better understand gall functioning and to develop new control strategies for this pest, it is essential to find out how the plant host cell cycle p...
Article
BACKGROUND: The hemolymph and the insect’s gut together play an essential role in the immune defense against microorganisms, including the production of antimicrobial peptides (AMP). AMPs are mainly induced by two specific signaling pathways, Toll and IMD (immune deficiency) pathways. Here, we have characterized the expression profile of four genes...
Article
Full-text available
The Myrtaceae family is an essential part of the worldwide economics, and it is present in different countries of Europe, Africa, Asia, and South America, with more than 133 genera and about 4,000 described species, from which Guinea guava (Psidium guineense) stands out in Brazil. This specie shows a potential use in different economic segments, su...
Preprint
Elevated CO2 (E[CO2]) improves the biomass and yield when combined with water-stress in C4 plants. Although several studies described the molecular response of the C4 plant Sorghum bicolor during drought exposure, none reported its combinatorial effect with E[CO2] in the roots. We decided to perform a molecular analysis using green prop roots, the...
Article
Meloidogyne incognita is the most frequently reported species in the root-knot nematode (RKN) complex responsible for damage to several different crops worldwide. The interaction between M. incognita and host plants involves the secretions of molecular factors from the nematode, which mainly suppress the defense response and promote plant parasitis...
Article
RNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small i...
Article
Full-text available
RNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small i...
Article
Full-text available
The cotton boll weevil, Anthonomus grandis, is the most economically important pest of cotton in Brazil. Pest management programs focused on A. grandis are based mostly on the use of chemical insecticides, which may cause serious ecological impacts. Furthermore, A. grandis has developed resistance to some insecticides after their long-term use. The...
Article
Full-text available
Plant-parasitic nematodes cause extensive annual yield losses to worldwide agricultural production. Most cultivated plants have no known resistance against nematodes and the few bearing a resistance gene can be overcome by certain species. Chemical methods that have been deployed to control nematodes have largely been banned from use due to their p...
Book
Full-text available
Capítulo 1: Introdução à edição genômica em plantas; Capítulo 2: Edição de genoma via non-homologous end joining (NHEJ) e ribonucleoproteínas (RNP); Capítulo 3: Edição de genoma por CRISPR/Casvia recombinação homóloga; Capítulo 4: Modulação da expressão gênica em plantas via tecnologia CRISPR/dCas9; Capítulo 5: Regulamentação da edição genômica em...
Preprint
Full-text available
Plant-parasitic nematodes cause expressive annual yield losses to worldwide agricultural production. Most cultivated plants have no known resistance against nematodes and the few bearing a resistance gene can be overcome by certain species. The chemical methods that have been deployed to control nematodes were largely banned from use due to their p...
Article
Full-text available
Gossypium hirsutum is the most important cotton species that is cultivated worldwide. Although cotton reproductive development is important for fiber production, since fiber is formed on the epidermis of mature ovules, cotton floral development remains poorly understood. Therefore, this work aims to characterize the cotton floral morphoanatomy by p...
Article
Full-text available
The sugarcane giant borer (SGB), Telchin licus licus, is a pest that has strong economic relevance for sugarcane producers. Due to the endophytic behavior of the larva, current methods of management are inefficient. A promising biotechnological management option has been proposed based on RNA interference (RNAi), a process that uses molecules of do...
Article
Full-text available
Plants are sessile organisms, which are vulnerable to environmental stresses. As such, plants have developed multiple molecular, physiological, and cellular mechanisms to cope with natural stressors. However, these environmental adversities, including drought, are sources of the main agribusiness problems since they interfere with plant growth and...
Article
Full-text available
The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Here...
Article
Full-text available
The sugarcane borer (Diatraea saccharalis, Fabricius, 1794) is a devastating pest that causes millions of dollars of losses each year to sugarcane producers by reducing sugar and ethanol yields. The control of this pest is difficult due to its endophytic behavior and rapid development. Pest management through biotechnological approaches has emerged...
Article
Full-text available
Rice blast disease is a major threat to rice production worldwide; the causative pathogenic fungus Magnaporthe oryzae induces rice (Oryza sativa) plants to undergo molecular changes that help them to circumvent this fungal attack. Transcriptome studies have demonstrated that many genes are involved in the defense response of rice to M. oryzae, but...
Article
Full-text available
Root-knot nematodes (RKNs, genus Meloidogyne) affect a large number of crops causing severe yield losses worldwide, more specifically in tropical and sub-tropical regions. Several plant species display high resistance levels to Meloidogyne, but a general view of the plant immune molecular responses underlying resistance to RKNs is still lacking. Co...
Article
Full-text available
Climate change and the exploration of new areas of cultivation have impacted the yields of several economically important crops worldwide. Both conventional plant breeding based on planned crosses between parents with specific traits and genetic engineering to develop new biotechnological tools (NBTs) have allowed the development of elite cultivars...
Preprint
Full-text available
Background: Meloidogyne incognita is the most frequently reported species from the root-knot nematode (RKN) complex responsible for causing damage in several different crops worldwide. The interaction between M. incognita and host plants involves the secretions of molecular factors from the nematode, which mainly suppress the defense response and p...
Article
Full-text available
Meloidogyne incognita is a plant-parasitic root-knot nematode (RKN, PPN) responsible for causing damage to several crops worldwide. In Caenorhabditis elegans, the DAF-16 and SKN-1 transcription factors (TFs) orchestrate aging, longevity, and defense responses to several stresses. Here, we report that MiDaf16-like1 and MiSkn1-like1, which are orthol...
Article
Full-text available
Setaria viridis, a C 4 monocot, was proposed as a model plant for studies on the response to stress conditions. Water-deficit ranks among the top three most devastating stresses and its importance will likely increase in the scenario of climate change. The aim of this work was to evaluate physiological and molecular water-deficit responses of S. vi...
Article
Mosses have long been recognized as powerful experimental tools for the elucidation of complex processes in plant biology. Recent increases in the availability of sequenced genomes and mutant collections, the establishment of novel technologies for targeted mutagenesis, and the development of viable protocols for large-scale production in bioreacto...
Article
Full-text available
Main conclusion The structure of the cotton uceA1.7 promoter and its modules was analyzed; the potential of their key sequences has been confirmed in different tissues, proving to be a good candidate for the development of new biotechnological tools. Abstract Transcriptional promoters are among the primary genetic engineering elements used to cont...
Article
Full-text available
The cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high...