Maria Coronada Fernández Calderón

Maria Coronada Fernández Calderón
Universidad de Extremadura | UNEX · Department of Biomedical sciences

PhD

About

38
Publications
3,822
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548
Citations
Introduction
My research activity is focused mainly on biofilm-based healthcare-associated infections and the strategies to detect and prevent the development of microbial biofilms in biomaterials for medical devices.
Additional affiliations
July 2012 - present
Universidad de Extremadura
Position
  • Professor (Associate)
January 2010 - present
Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN)
Position
  • Researcher

Publications

Publications (38)
Article
Full-text available
Bacterial infection represents a major cause of implant failure in dentistry. A common approach to overcome this issue and treat peri-implant infection consists in the use of antibiotics. However, the rise of multidrug resistant bacteria poses serious concerns to this strategy. A promising alternative is the use of antimicrobial peptides due to the...
Article
Full-text available
Polylactic acid/Mg composites have been recently proposed for biodegradable osteosynthesis devices because, with regards to the neat polymer, they combine an enhanced biocompatibility and bioactivity with better mechanical properties, particularly creep strength. A question still arises about their bacterial behavior. For this purpose, composites o...
Article
In this study, the influence of nanometer scale roughness on bacterial adhesion and subsequent biofilm formation has been evaluated using spatially organized microtopographic surface patterns for four major opportunistic pathogens of the genus Staphylococcus (S. epidermidis and S. aureus) responsible for associated-biofilm infections on biomedical...
Article
New biocompatible and bioabsorbable materials are currently being developed for bone regeneration. These serve as scaffolding for controlled drug release and prevent bacterial infections. Films of polylactic acid (PLA) polymers that are Mg-reinforced have demonstrated they have suitable properties and bioactive behavior for promoting the osseointeg...
Article
Magnesium is currently increasing interest in the field of biomaterials. An extensive bibliography on this material in the last two decades arises from its potential for the development of biodegradable implants. In addition, many researches, motivated by this progress, have analyzed the performance of magnesium in both in vitro and in vivo assays...
Article
El objetivo de este trabajo ha sido estudiar si los tratamientos térmicos y de conversión química mejoran la biocompatibilidad de la aleación TiAlV y reducen el crecimiento bacteriano. En primer lugar, se modificó la aleación de TiAlV mediante tratamiento térmico a 650 ºC durante 1 hour. Luego, se llevó a cabo la conversión química en una solución...
Article
Full-text available
Increased bacterial resistance to traditional antimicrobial agents has prompted the use of natural products with antimicrobial properties such as propolis, extensively employed since ancient times. However, the chemical composition of propolis extracts is extremely complex and has been shown to vary depending on the region and season of collection,...
Article
In this paper, a simple numerical procedure is presented to monitor the growth of Streptococcus sanguinis over time in the absence and presence of propolis, a natural antimicrobial. In particular, it is shown that the real-time decomposition of growth curves obtained through optical density measurements into growth rate and acceleration can be a po...
Article
Background Hypercholesterolemia is one of the principal causes of the development of cardiovascular diseases. Recently, probiotics consumption has been also proposed as a non-pharmacological intervention to control cholesterol concentrations. Objective To evaluate in vitro assimilation of cholesterol by Bifidobacterium animalis subsp. lactis (BPL1...
Article
Diabetes is a widely spread disease affecting the quality of life of millions of people around the world and is associated to a higher risk of developing infections in different parts of the body. The reasons why diabetes enhances infection episodes are not entirely clear; in this study our aim was to explore the changes that one of the most freque...
Article
Full-text available
As part of our ongoing studies related with the coordination chemistry of 2-thiazoline derivatives with transition ions, we report the synthesis of a new class of structurally novel complexes containing in their structures a 2-aminobenzimidazole ring. The resulting compound, 2-(2-aminobenzimidazole-1-yl)-2-thiazoline (BzTn), and two cobalt(II) comp...
Article
Full-text available
Background Resistance to traditional antifungal agents is a considerable health problem nowadays, aggravated by infectious processes related to biofilm formation, usually on implantable devices. Therefore, it is necessary to identify new antimicrobial molecules, such as natural products, to develop new therapeutic strategies to prevent and eradicat...
Article
In the present study, an effort has been made to understand the interaction mode of propolis, a natural substance produced by honey bees, with gram-positive and gram-negative bacterial cells by measuring alterations in cell surface physico-chemical properties following the incubation of the cells with different sub-inhibitory concentrations of this...
Article
Full-text available
Propolis is a natural product obtained from hives. Its chemical composition varies depending on the flora of its surroundings, but nevertheless, common for all types of propolis, they all exhibit remarkable biological activities. The aim of this study was to investigate the chemical composition and antimicrobial activity of a novel Spanish Ethanoli...
Article
Laser shock processing (LSP) is increasingly applied as an effective technology for improving the properties of different metallic components. This is done principally to enhance their corrosion and fatigue life behaviour, stress corrosion cracking resistance, etc. In this paper, LSP has been applied to a commercially pure Mg and a Mg-1Zn alloy (wt...
Article
Full-text available
Dental implantology allows replacement of failing teeth providing the patient with a general improvement of health. Unfortunately not all reconstructions succeed, as a consequence of the development of infections of bacterial origin on the implant surface. Surface topography is known to modulate a differential response to bacterial and mammalian ce...
Article
Full-text available
Candida tropicalis is an emergent pathogen with a high rate of mortality associated with it; however, less is known about its pathogenic capacity. Biofilm formation (BF) has important clinical repercussions, and it begins with adherence to a substrate. The adherence capacity depends principally on the cell surface hydrophobicity (CSH) and, at a lat...
Article
TaN(Ag) composited coatings are being investigated to improve biocompatibility of different biomedical devices due to the mechanical and chemical stability of TaN and bactericidal effect of silver nanoparticles. However, controlling the size, density, shape and especially the release of silver ions (Ag) into the surrounding medium becomes a challen...
Poster
Many dental implants fail due to the infection and inflammation that walk hand in hand with poor healing and soft tissue integration. Therefore, the development of antibacterial, anti-inflammatory and tissue-regenerative surface modifications constitutes a major challenge to guarantee implant success. In previous studies, we screened among differen...
Article
Myo-inositol hexaphosphate, also called phytic acid or phytate (IP6), is a natural molecule abundant in vegetable seeds and legumes. Among other functions, IP6 inhibits bone resorption. It is adsorbed on the surface of hydroxyapatite, inhibiting its dissolution, and decreasing the progressive loss of bone mass. We present here a method to directly...
Article
Full-text available
Many dental implants fail due to the infection and inflammation that walk hand in hand with poor healing and soft tissue integration. Titanium surfaces were nanocoated with quercitrin, a natural flavonoid, with the aim to improve soft tissue integration and increase dental implants success. Streptococcus mutans attachment and biofilm formation was...
Article
Full-text available
Aim: The aim of the present study was to report main topographical and chemical changes of a failing 18-year in function retrieved acid etching implant in the micro- and nano-scales.Material and methods: A partially edentulous 35-year old male rehabilitated eighteen years ago with dental implant in the upper maxilla exhibit implant mobility. After...
Poster
Background: Endosseous dental implants based on titanium are increasingly used in clinical dentistry due to their excellent biocompatibility in contact with bone and resistance to corrosion. Surface properties of this kind of implants are known as essential factors for successful osseointegration. Therefore, implant chemical composition and implant...
Poster
Improvement of the functionality of metallic implants is achieved by the coverage of its surface with active molecules that enhances its biocompatibility. However, because of the low reactivity of the Ti6Al4V surface, a strategy for an efficient anchoring of these molecules to the surface is its silanization. One of the most serious problems that c...
Article
Full-text available
Objective: To report the main topographical features in the micro- and nano-scales and to assess implant chemical changes of the surface of a 3-year clinically retrieved oxidized titanium dental implant, and compare them with a similar, unused implant. Materials and methods: The surface of the oxidized titanium dental implants was assessed by su...
Conference Paper
Full-text available
INTRODUCTION Bacterial infection represents a major cause of implant failure in dentistry1. A common strategy to treat peri-implant infection is the use of antibiotics; however, the rise of multidrug resistant bacteria poses serious concerns to this strategy2. A promising alternative is the use of antimicrobial peptides (AMP) due to their broad-spe...
Poster
Full-text available
Laser peening improves the surface of stainless steel 316LVM against S. epidermidis ATCC 35983. However if bacteria are able to generate a high amount of slime (S. epidermidis ATCC 35984), the behaviour of treated surfaces is similar to the control one. Ti6Al4V behaves quite similarly to steel, suggesting that the modification of the surface produc...
Article
Formation of thin films on titanium alloys incorporating bioactive small molecules or macromolecules is a route to improve their biocompatibility. Aminoalkylsilanes are commonly employed as interface reagents that combine good adhesion properties with an amino tail group susceptible of further functionalization. This article introduces a reproducib...
Poster
Full-text available
Titanium and titanium alloys are widely employed in biomedical implants due to their chemical inertia and biocompatibility.[1] The latter is generally improved by forming thin films of bioactive small molecules on the metal surface, thus preventing bacterial adhesion which represents a major concern. A common chemical functionalization of such allo...
Article
The new ligand 2-acetyl-2-thiazoline thiosemicarbazone (HATtsc) and the complexes [Co(ATtsc)(2)](2)[CoCl(4)]·2H(2)O (1), [Co(ATtsc)(2)]NO(3)·H(2)O (2), [Ni(HATtsc)(2)](NO(3))(2) (3), [ZnCl(2)(HATtsc)]·CH(3)CN (4), [{CdCl(HATtsc)}(2)(μ-Cl)(2)]·2H(2)O (5) and [{Cd(NO(3))(HATtsc)}(2)(μ-NO(3))(2)] (6) were isolated and characterized by a variety of phy...
Article
TiO(2)-coated biomaterials that have been excited with UV irradiation have demonstrated biocidal properties in environmental applications, including drinking water decontamination. However, this procedure has not been successfully applied towards the killing of pathogens on medical titanium-based implants, mainly because of practical concerns relat...
Poster
Full-text available
INTRODUCTION The austenitic stainless steel 316LVM is a biomaterial that combines good mechanical properties with a reasonable biocompatibity. However, different surfaces treatments are developed for improving such mechanical properties, by also considering their possible effects in the biological response of the implant. One of the main biological...
Article
Full-text available
Secretion of hydrolytic enzymes is considered a virulence factor in Candida spp. Extracellular enzymatic activities in 29 clinical isolates of Candida tropicalis were analyzed by plate assays. C. tropicalis, similar to Candida albicans, showed elevated hemolytic and esterase activities. However, unlike C. albicans, low aspartyl protease and very lo...
Poster
Full-text available
Introduction. Ti6Al4V is one of the most commonly used orthopaedic implants due to its good biocompatibility. However, bacterial adhesion to its surface and the subsequent biofilm growing is one of the main reasons for its failure after surgery due to the development of infections. By this reason, the objective of this study is to design an experim...
Article
Full-text available
We have evaluated the cellular surface hydrophobicity (CSH) determination as an additional criterion to differentiate Candida albicans from Candida dubliniensis. Our results show that C. albicans when grown at 37 degrees C in Sabouraud is always hydrophilic, and C. dubliniensis presents high CSH levels.

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Projects

Projects (5)
Project
The increasing resistance to antibiotics, which is considered by the WHO to be one of the greatest challenges to global health, food security and development. This resistance to antibiotics is accelerated by the misuse and abusive use of these drugs and by failures to prevent and control infections. For these reasons, there is an emergency to search antibacterial natural substances that do not generate resistance and that are capable of eliminating or preventing infections related to the biofilm production, either individually or in synergy with other compounds.
Archived project
Project
Oral cavity infections associated with polymicrobial biofilms are a serious problem nowadays. The microorganisms adhere to the surfaces of the cavity and can form biofilms that protect them from host defenses and antimicrobial treatment. This peculiar mode of bacteria growth within biolayers results in the development of infectious processes that are difficult to eradicate. The project goal is focused on preventing these infections using natural substances and determine its potential clinical utility.