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Maria Cristina Gagliano

Maria Cristina Gagliano
Wetsus · Department of Microbiology

PhD in Environmental Science
Scientific project manager and Theme Coordinator

About

34
Publications
14,869
Reads
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558
Citations
Introduction
Maria Cristina Gagliano currently works at Wetsus, european centre of excellence for sustainable water technology. Her research focus on many aspects of Water Treatment and Environmental Micriobiology, with particular attention on Anaerobic Microbiology, EPS production and Biofilm growth. Main methods involve fluorescence and confocal microscopy investigations to explore the function of complex microbial communities in several lab-scale setup. At Wetsus, she is also Theme Coordinator for the topic Biofilms, making connections between water companies and universities to build-up effective solutions for problems related to the the drinking water distribution networks.
Additional affiliations
October 2009 - June 2014
Italian National Research Council
Position
  • Research Associate

Publications

Publications (34)
Article
Thermophilic bacteria have recently attracted greater attention because of their potential application in improving different biochemical processes like anaerobic digestion of various substrates, wastewater treatment or hydrogen production. In this study we report on the design of a specific 16S rRNA-targeted oligonucleotide probe for detecting mem...
Article
Thermal hydrolysis pretreatment coupled with Thermophilic Anaerobic Digestion (TAD) for Waste Activated Sludge (WAS) treatment is a promising combination to improve biodegradation kinetics during stabilization. However, to date there is a limited knowledge of the anaerobic biomass composition and its impact on TAD process performances. In this stud...
Article
Full-text available
For the anaerobic biological treatment of saline wastewater, Anaerobic Digestion (AD) is currently a possibility, even though elevated salt concentrations can be a major obstacle. Anaerobic consortia and especially methanogenic archaea are very sensitive to fluctuations in salinity. When working with Upflow Sludge Blanket Reactor (UASB) technology,...
Article
Full-text available
Anaerobic granulation at elevated salinities has been discussed in several analytical and engineering based studies. They report either enhanced or decreased efficiencies in relation to different Na+ levels. To evaluate this discrepancy, we focused on the microbial and structural dynamics of granules formed in two upflow anaerobic sludge blanket (U...
Article
Full-text available
In the recent years anaerobic sludge granulation at elevated salinities in upflow anaerobic sludge blanket (UASB) reactors has been investigated in few engineering based studies, never addressing the microbial community structural role in driving aggregation and keeping granules stability. In this study, the combination of different techniques was...
Article
Full-text available
Recently, methanogenic archaea belonging to the genus Methanothrix were reported to have a fundamental role in maintaining stable ecosystem functioning in anaerobic bioreactors under different configurations/conditions. In this study, we reconstructed three Methanothrix metagenome-assembled genomes (MAGs) from granular sludge collected from saline...
Article
Full-text available
Soluble Extracellular Polymeric Substances (sEPS) are a mixture of microbial soluble polymers produced during aerobic or anaerobic treatment of wastewater. Gel layers mainly consisting of sEPS are usually present in the fouling layers of membrane bioreactors (MBRs) and in the dynamic membranes (DMs) of dynamic membrane bioreactors (DMBRs), and thei...
Article
Full-text available
Reverse electrodialysis (RED) is a process to harvest renewable energy from the salinity gradient obtained by the controlled mixing of river and seawater. When using natural waters, (bio)fouling is an inevitable process which has a negative impact on the obtained power density. Specific characteristics of RED do not allow the direct transfer of kno...
Article
Full-text available
This paper aims to elucidate the role of extracellular polymeric substances (EPS) in regulating anion and cation concentrations and toxicity towards microorganisms in anaerobic granular sludges adapted to low (0.22 M of Na⁺) and high salinity (0.87 M of Na⁺). The ion exchange properties of EPS were studied with a novel approach, where EPS were enta...
Article
The potential effect of different Ca2+ additions (150, 300, 450, 600 and 1000 mg/L) on microbial activity and aggregation, during anaerobic digestion at moderate (8 g/L Na+) and high salinity (20 g/L Na+) has been investigated. Batch tests were carried out in duplicate serum bottles and operated for 30 days at 37 °C. At 8 g/L Na+, methanogenic acti...
Article
Full-text available
Microbial extracellular polymeric substances (EPS)were produced in two membrane bioreactors, each separately treating fresh and saline synthetic wastewater (consisting of glycerol and ethanol), with the purpose of applying them as sustainable bioflocculants. The reactors were operated under nitrogen-rich (COD/N ratios of 5 and 20)and limited (COD/N...
Article
Full-text available
Increasing amounts of saline (waste)water with high concentrations of organic pollutants are generated globally. In the anaerobic (waste)water treatment domain, high salt concentrations are repeatedly reported to inhibit methanogenic activity and strategies to overcome this toxicity are needed. Current research focuses on the use of potential osmol...
Article
A system using a two-phase anaerobic configuration (mesophilic/thermophilic) was tested by feeding waste activated sludge (WAS). The first acidogenic stage presented a hydraulic retention time (HRT) of 3 days, while the second methanogenic stage had an HRT of 10 days. Both raw and ultrasonically pretreated WAS samples were utilized for the experime...
Article
Full-text available
It is commonly accepted that high salt concentrations negatively affect microbial activity in biological wastewater treatment reactors such as upflow anaerobic sludge blanket (UASB) reactors. Microbial aggregation in such reactors is equally important. It is well documented that anaerobic granules, when exposed to high salinity become weak and disi...
Poster
Full-text available
A system using an original two-phase anaerobic configuration (mesophilic/thermophilic) was implemented to study the effects of volatile fatty acids (VFAs) accumulation under thermophilic conditions; the system was fed with waste activated sludge (WAS), raw and ultrasonically pre-treated. The first acidogenic stage presented a hydraulic retention ti...
Article
Full-text available
Methanogenic biomass plays a key role with regards methane production during anaerobic bioconversion of organic substrates. In this study, the effect of two different acclimated inocula on digestion performances was investigated by means of anaerobic batch tests on untreated and sonicated waste activated sludge. Organics solubilisation and removal...
Article
This work aimed to isolate and characterize organisms able to store polyhydroxyalkanoates (PHAs), from a mixed microbial culture (MMC) selected under feast and famine conditions, using hardwood sulfite spent liquor (HSSL), a by-product of the pulp and paper industry, as the carbon source. The MMC was able to accumulate PHA from the different HSSL c...
Research
Full-text available
Novel processing routes for effective sewage sludge management - Enhanced stabilization processes
Article
Full-text available
Thermophilic bacteria have been isolated from several terrestrial, marine and industrial environments. Anaerobic digesters treating organic wastes are often an important source of these microorganisms, which catalyze a wide array of metabolic processes. Moreover, organic wastes are primarily composed of proteins, whose degradation is often incomple...
Article
Anaerobic digestion (AD) is one of the few sustainable technologies that both produce energy and treat waste streams. Driven by a complex and diverse community of microbes, AD may be affected by different factors, many of which also influence the composition and activity of the microbial community. In this study, the biodiversity of microbial popul...
Article
The requirement for enhanced stabilization processes to obtain a more stable, pathogen-free sludge for agricultural use is an increasing challenge to comply with in the waste hierarchy. With this in mind, the Routes European project ('Novel processing routes for effective sewage sludge management') is addressed to assess innovative solutions with t...
Article
Full-text available
Ultrasounds pretreatment aims to convert waste activated sludge, characterized by recalcitrant particulate organics, into a soluble, more biodegradable form, maximizing anaerobic stabilization and biogas recovery. Objective of this work was the assessment of the efficiency of high frequency ultrasounds, as 200 kHz, to improve sludge anaerobic diges...
Article
In this work the potential of high frequency ultrasounds as pretreatment for sludge anaerobic digestion has been assessed. Irradiation with 200kHz ultrasounds was efficient in disintegrating the floc structure increasing the available fraction of soluble organic matter (up to seven times at 25,000kJ/kgTS). Batch anaerobic digestion tests were carri...

Questions

Question (1)
Question
Hello everyone,
I had multiple PCR products after doing the colony PCR on white colonies (primers SP6-T7, annealing temp 55 degrees) which are supposed to contain only one insert. I cloned a bacterial 16S (about 1500 bp), into a Promega pGEM vector (about 3000 bp). The previous analysis about the 16S cloned insert (gel visualization, purification, concentration measurement) were ok.  The cloning procedure was ok (as usual). 
As you can see in the photo, in lane 4, 5 and 8, besides the 1500 bp insert there is a smaller one at 1000 bp and a bigger insert above 3000 bp.In lane 8 the bigger inserts were three... (The marker is Generuler 1 kb)
I have some ideas about the reason but I'm curious to know your experience about this.
Thank you for your replies
Cristina

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Projects

Projects (4)
Project
1. Improve the performance of Lt-AD on lipids-rich wastewater 2. Understand the microbial ecology of the Lt-AD lipids process