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Publications (37)
p>Modern mentality tends to minimize what is real to a physical world that is accessible to its senses, instruments, reasoning and equations, ignoring other states of reality that, clearly throughout humanity’s history have been known. Modern human believes that he is capable of dispensing all knowledge from what he has been taught in the past by s...
Modern mentality tends to minimize what is real to a physical world that is accessible to its senses, instruments, reasoning and equations, ignoring other states of reality that, clearly throughout humanity's history have been known. Modern human believes that he is capable of dispensing all knowledge from what he has been taught in the past by sta...
According to Mayan cosmology, the law of time is represented as the energy factored by time equals to art. Time is a form of biological information. Time in-forms life, in such a way that life forms processes time as information and externalize it specific forms into the three-dimensional world, then time is the principle ordering of life. Indeed,...
We grow up in a perspective of an existence, which we call reality. We identify ourselves with this reality as something absolute and we reject the experiences that do not belong to it. Each reality is valid only within certain limits (such as family's traditions, religious beliefs, culture etc). So, "this is how our own state of consciousness perc...
In this manuscript we describe a biological system that deals with herpes simplex virus in the context of an in vitro infection in the presence of carrageenan, from a novel point of view by comparing our system to the Ligeti's symphonic poem of 100 metronomes. Metronomes (from ancient Greek μέτρον-métron, "measure" and νέμω-némo, "I manage", "I lea...
A new paradigmatic understanding of evolution, genetic novelty, code-generating, genome-formatting factors, infectious RNA Networks, viruses and other natural genetic content operators
Our perspective on nature has changed throughout history and at the same time has affected directly or indirectly our perception of biological processes. In that sense, the “fluxus” of information in a viral population arises a result of a much more complex process than the encoding of a protein by a gene, but as the consequence of the interaction...
Natural polysaccharides known as carrageenans are potent and selective inhibitors of herpes simplex virus through blocking the interaction of the virus with the cellular receptor. Passaging the virus in vitro in the presence of carrageenans types k and ι enabled us to generate variants of herpes simplex type 2. At passage 22, four clones were selec...
The understanding of the processes occurring in Nature has been a continuing concern throughout the history of mankind. Intellectual tools employed towards this goal were specific for each period and have been largely based on the prevailing paradigms that reigned in the past. In this work we present evidence that supports the idea of viruses as ke...
Immune evasion strategies are important for the onset and the maintenance of viral infections. Many viruses have evolved mechanisms to counteract or suppress the host immune response. We have previously characterized two syncytial (syn) variants of Herpes simplex 1 (HSV-1) strain F, syn14-1 and syn17-2, obtained by selective pressure with a natural...
The structures of two sulfated xylomannans extracted from the red alga Nemalion helminthoides were determined. These two fractions plus a sulfated mannan, isolated from the same alga and whose structure was previously reported, were subjected to chemical modification. The mannan was oversulfated with SO(3)-pyridine in dimethyl sulfoxide at 60 °C du...
Antiviral therapy against herpes simplex virus based on sulfated polysaccharides, like carrageenans, represents a new alternative for genital herpes infections treatment and arises the concern about the appearance of resistant viral populations.
We characterized the F strain of herpes simplex virus-1 passaged in the presence of a natural carrageena...
Despite the advances in biomedical knowledge, there remain many challenging and significant unsolved problems among which are included viral pathogenesis and antiviral therapy, as main topics in human health. On this respect, for instance, our knowledge about human immunodeficiency virus and AIDS is still insufficient to deal with problems of immen...
Nemalion helminthoides, collected in the Argentine South Atlantic coast, was extracted with hot water and the crude product fractionated using cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration and seven fractions were separated and analyzed. Structural analysis of t...
In this study, we have analyzed water-extracted polysaccharides of Gracilaria corticata. The water extract (WE), a galactan-containing sub-fraction (F3) and their hyper sulfated derivatives (WES1, WES2, F3S1 and F3S2) had anti-HSV activity with inhibitory concentration 50% (IC50) from 1.1 to 27.4 microg/ml. Sub-fraction F3, which has a molecular ma...
Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fra...
The inhibitory action of polyanionic substances on virus replication was reported more than 50years
ago. Seaweeds, marine invertebrates, and higher plants represent abundant sources of novel compounds of
proved antiviral activity. Natural sulfated polysaccharides (SPs) are potent in vitro inhibitors of awide
variety of enveloped viruses, such as h...
The acidic vaginal milieu is presumed to inactivate pathogens but is neutralized by semen. This notion fostered the development
of acid-buffering products, such as ACIDFORM (developed by Program for Topical Prevention of Conception and Disease, Rush
University, and licensed by Instead), as microbicides. However, the extent and mechanism of protecti...
Prevention of genital herpes and other sexually transmitted infections (STI) is a critical health priority because of the
overwhelming impact on women and infants and the epidemiological association with human immunodeficiency virus (HIV)/AIDS.
Small animal models are essential for evaluating strategies for prevention or treatment of STI. Neither t...
Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage
(CVL) samples were obtained from 20 healthy women and evaluated for anti–herpes simplex virus (HSV) activity. CVL samples
reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the c...
Genital herpes is one of the most prevalent sexually transmitted infections worldwide and is the most common cause of genital ulcers. Despite increased public awareness and the initiation of efforts to prevent transmission, the prevalence of herpes simplex virus (HSV) type 2 continues to increase. What makes HSV so difficult to control is that most...
A sulfated polysaccharide fraction was isolated from the hot water extract of the green alga Caulerpa racemosa and designated HWE. This polymer, which contained galactose, glucose, arabinose and xylose as the major component sugars, had [alpha](D)(30) + 46.2 degrees in water and contained 9% sulfate hemiester groups. Sugar linkage analysis indicate...
In the present study, the protective effect of 1T1, a lambda-carrageenan extracted from the red seaweed Gigartina skottsbergii was evaluated in a murine model of herpes simplex virus type 2 (HSV-2) genital infection. Six to eight-week-old female BALB/c mice were intravaginally inoculated with a lethal dose of HSV-2 (MS strain) and pre- or post-infe...
Background: There is an urgent need to develop topical microbicides that protect against sexually transmitted infections (STI). The human vagina has a pH range of 3.5-4.5, which is presumed to inactivate STI pathogens. This acidity is neutralized by alkaline semen for up to 6 h following intercourse. The notion that acidic pH may be protective has...
The inhibitory effect of several thiosemicarbazones (TSCs), synthesized from aromatic ketones and terpenones, and their heterocyclic thiadiazoline (TDZ) derivatives, was investigated against Junin virus (JUNV), an arenavirus agent of Argentine haemorrhagic fever. From the 25 compounds tested, six compounds belonging to the TSC group were found to b...
Polysaccharides were sequentially extracted from the agarophyte Gracilaria corticata. Chemical analysis combined with infrared spectroscopy showed that the cold water extracted material consists mainly of a high molecular weight sulfated galactan. Most of the sulfate groups are alkali labile and are located at C-4 of the 1,3-linked D-galactose unit...
Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii were recently identified as potent and selective inhibitors of herpes simplex virus types 1 and 2 (HSV-1 and -2). The mu/nu-carrageenan 1C3 was tested in vitro for its ability to select resistant variants. After serial passages of HSV-1 strain F in...
A novel series of DL-galactan hybrids extracted from the red seaweed Gymnogongrus torulosus, was evaluated for its in vitro antiviral properties against herpes simplex virus type 2 (HSV-2) and dengue virus 2 (DEN-2). These compounds were very active against both viruses with inhibitory concentration 50% (IC50) values in the range 0.6-16 microg/ml f...
Carrageenans extracted from cystocarpic and tetrasporic Stenogramme interrupta were analysed by chemical and spectroscopic methods. The carrageenan from cystocarpic plants is composed predominantly of 0.5 M KCl-insoluble and 1 M KCl-soluble fractions. The insoluble fraction contained iota-carrageenan as the major component with alpha-carrageenan an...
The effects of the carrageenans 1T1 (lambda-type), 1C1 (kappa/iota-type) and 1C3 (upsilon/nu-type), isolated from the red seaweed Gigartina skottsbergii, on herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection of murine astrocytes were investigated. The three compounds were effective inhibitors of virus replication, as determined by a v...
The lambda-carrageenan 1T1, the kappa/iota-carrageenan 1C1 and the mu/nu-type 1C3, isolated from the red seaweed Gigartina skottsbergii, proved to be potent and selective inhibitors of herpes simplex virus (HSV) types 1 and 2. The antiviral IC50 values determined by virus yield inhibition assay in different cell lines ranged from 0.4 to 3.3 microg/...
A sulphated galactan (SG) with low molecular weight (app. 2800) was isolated from extracts of Cryptopleura ramosa, a red seaweed from the South American coasts. The compound was a selective inhibitor of HSV-1 and HSV-2 replication in Vero cells with 50% inhibitory concentrations (IC50) in the range 1.6-4.2 micrograms/ml and a 50% cytotoxic concentr...
The antiviral activity against herpes simplex virus types 1 and 2 of kappa/l-, partially cyclized mu/v-, and lambda-carrageenans isolated from the red seaweed Gigartina skottsbergii and their cyclized derivatives was analyzed. lambda-Carrageenans and the partially cyclized mu/v-carrageenan were the most potent inhibitors of herpes viruses (includin...
