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April 2001 - present
Education
September 1991 - September 1997
Publications
Publications (66)
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This review elucidates the transformative impact of artificial intelligence (AI) on viticulture, showcasing its practical applications in disease prediction, pest management, automated grape harvesting, and the optimization of water and nutrient management. The implementation of AI-driven technologies enables vineyard managers...
This review explores the transformative role of artificial intelligence (AI) in the entire winemaking process, from viticulture to bottling, with a particular focus on enhancing food safety and traceability. It discusses AI’s applications in optimizing grape cultivation, fermentation, bottling, and quality control, while emphasizing its critical ro...
Artificial intelligence (AI) is revolutionizing approaches in plant disease management and phytopathological research. This review analyzes current applications and future directions of AI in addressing evolving agricultural challenges. Plant diseases annually cause 10–16% yield losses in major crops, prompting urgent innovations. Artificial intell...
Artificial intelligence (AI) is revolutionizing approaches in plant disease management and phy-topathological research. This review analyzes current applications and future directions of AI in addressing evolving agricultural challenges. Plant diseases annually cause 10-16% yield losses in major crops, prompting urgent innovations. Artificial intel...
Agriculture currently confronts a multitude of challenges arising from the excessive utilization of chemical pesticides and the proliferation of phytopathogenic fungi strains that exhibit resistance to commonly employed active compounds in the field. Botrytis cinerea and Colletotrichum acutatum are phytopathogenic fungi that inflict substantial eco...
This manuscript presents a 4 years in-situ evaluation of TiO 2 /SiO 2-based product applied to a mortar seating belonging to the bleachers of the Cadiz Roman Theatre, affected by biofouling and organic deposits. Antifouling performance was studied by colorimetric measurements and ATP analyses. Surface sampling was performed for biofouling identific...
Polyhydroxyalkanoates (PHAs) production from lignocellulosic biomass using mixed microbial cultures (MMC) is a potential cheap alternative for reducing the use of petroleum-based plastics. In this study, an MMC adapted to acidogenic effluent from dark fermentation (DF) of exhausted sugar beet cossettes (ESBC) has been tested in order to determine i...
Building materials decay is caused by the combination of chemical, physical and biological aspects. Therefore, the development of innovative multifunctional building materials is an alternative to reduce their impact. This can be achieved by post-treatments or by direct changes in the fabrication of synthetic materials, such as engineered stone. Th...
The genus Phytophthora with more than 100 described species and 58 officially recognized, phylogenetically distributed in ten clades, are important pathogenic oomycete chromists that cause important diseases in agricultural crops, trees and forests worldwide. This genus is known as \"The Plant Destroyer” which causes great economic losses with cost...
During the infection of grapevine (Vitis vinifera) by the fungus Botrytis cinerea, the concentration of polyamines, which are toxic substances for the phytopathogen, increases in the grape. Nine NRPS genes have been identified in the genome of B. cinerea, yet the function of five of them remains unknown. For this reason, we have studied the express...
The authors wish to make it known that we mistakenly used some unpublished data from the Joint Genome Institute (JGI) database in some parts of this article in a way that violates the JGI usage agreement. We apologize to the principal investigators of the JGI Community Sequencing Projects (below) for this error.
Microbial fouling on the surface and water retention are common problems affecting porous ceramic materials (stone, concrete…) that can alter their properties, shorten their service life and even pose a health risk. Though proper maintenance (disinfection, drainage…) can mitigate these issues, it is a time and resource intensive process and prevent...
10.1. Introduction 157
10.1.1. Modern strawberry, a domesticated species for production 157
10.1.2. Economic importance of world strawberry production 158
10.1.3. Pathogen incidence in strawberry production 158
10.2. The Main Fungal Pathogens of Strawberry 159
10.2.1. Botrytis cinerea 162
10.2.2. Colletotrichum spp. 162
10.2.3. Fusarium oxysporium...
This book provides a broad, well-structured review of strawberries and their cultivation under current environmental conditions. Methods of strawberry cultivation have undergone many improvements, and this book covers aspects from plant propagation, architecture, genetic resources, breeding, abiotic stresses and climate change to evolving diseases...
Protective coatings with hydrophobic or biocide properties are commonly employed to prevent biofouling of building materials exposed to the environment. Although the factors affecting bioreceptivity of the base materials are well known, the influence of the coating and/or biocide agent surface properties on the interaction with the cell surface is...
Grapevine shoots, known to be a source of bioactive stilbenes, are considered one of the most important and abundant waste products from the vineyard. The objective of this study was to obtain a pure stilbene extract from grapevine shoots and test its properties, mainly antimicrobial ones, as a preservative in wine. A new extract with a purity of 9...
Controlled inoculations of non-Saccharomyces yeasts are becoming increasingly used to produce high-quality wines due to their enological potential. In this study, we evaluated the impact of sequential inoculation with the commercial non-Saccharomyces yeasts (Torulaspora delbrueckii and Metschnikowia pulcherrima) in combination with Saccharomyces ce...
The genus Colletotrichum is considered one of the most relevant plant pathogens insofar as it is capable of causing damage to a wide variety of herbaceous and woody plants. The genome of 25 species of Colletotrichum, including 28 different strains, has recently been published; 15 of these strains are still at the annotation stage and thirteen are n...
The alarming increase of pollution has significantly increased buildings maintenance. Nowadays, the economic figures associated to repairing activities are even more relevant than those corresponding to new construction works, especially on heritage buildings. Since the degradation of building materials is the result of a complex combination of phy...
The fungal genus Colletotrichum contains about 190 species, many of which are responsible for serious plant diseases including those of commercial crops. These species infect a wide range of crops in the tropical, sub-tropical and temperate regions of the world. The diseases caused by Colletotrichum species are known as “anthracnose diseases” and a...
A research on the production of lemon vinegar directly from lemon juice was conducted. For the alcoholic fermentation, two different strains of Saccharomyces cerevisiae, as well as two types of cultures (submerged culture and yeasts immobilized in alginate beads) were used. A microbiological control was also implemented during this stage. Acetic fe...
This study evaluates the impact on two varietal white wines from ‘Chardonnay’ and ‘Verdejo’ cultivars of different fermentative strategies: inoculation with Saccharomyces cerevisiae yeast (CT), sequential inoculation (Torulaspora delbrueckii / Saccharomyces cerevisiae) (SI), and spontaneous fermentation (SP). The wines’ chemical composition was cha...
Following a preliminary study to determine the possibility of using a grapevine shoot extract (VIN) as a sustainable alternative to sulfur dioxide (SO2), in this study, the chromatic features, phenolic composition, and sensory analysis of wines treated with VIN at two concentrations were studied during storage in bottle for the first time. The high...
The decay of building materials is generally caused by the combination of chemical, physical and biological agents. Therefore, the development of products that combine multiple protection mechanisms is desirable. We have developed, via a sol-gel route, CuO/SiO2 nanocomposites with application as a multifunctional protective treatment for building s...
Carbon capture and storage (CCS) in stable geological locations is one of the options to mitigate the negative effects of global warming produced by the increase in CO2 concentrations in the atmosphere. A CO2 leak is one of the risks associated with this strategy. Marine bacteria attached to the sediment may be affected by an acidification event. R...
Proteomics has become one of the most relevant high-throughput technologies. Several approaches have been used for studying, for example, tumor development, biomarker discovery, or microbiology. In this “post-genomic” era, the relevance of these studies has been highlighted as the phenotypes determined by the proteins and not by the genotypes encod...
Strawberry (Fragaria ananassa) is one of the world’s most commercially important fruit crops, and is grown in many countries The commercial viability of the crop is continually subject to various risks, one of the most serious of which is the diseases caused by phytopathogenic organisms. More than 50 different genera of fungi can affect this cultiv...
Pathogenic fungi in plants are now one of the most serious causes of crop losses in Europe. Phytopathogenic fungi are able to infect any tissue at any stage of plant growth. Most significantly, these organisms are able to maintain their infective capacity between seasons because sources of inoculum can remain present in soils and plant debris. The...
Botrytis cinerea is a phytopathogenic fungus infecting a number of crops (tomatoes, grapes and strawberries), which has been adopted as a model system in molecular phytopathology. B. cinerea uses a wide variety of infection strategies, which are mediated by a set of genes/proteins called pathogenicity/virulence factors. Many of these factors have b...
Colletotrichum acutatum is a major plant pathogen which infects a broad range of host plants. Extensive research has been carried out on C. acutatum populations affecting various hosts in different geographical locations, showing a considerable genotypic and phenotypic
diversity. Anthracnose, caused by Colletotrichum spp., is the major disease of c...
Real-time PCR (TaqMan®) assays were developed for the specific detection and discrimination of Colletotrichum spp., C. acutatum and C. gloeosporioides causing anthracnose in strawberry using the most divergent area of the internal transcribed spacers (ITS1 and ITS2) and 5·8S ribosomal RNA (rRNA) gene region. The specificity of the new assays was te...
Anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry, Fragaria × ananassa. This study identifies the Colletotrichum spp. which causes strawberry anthracnose in the southwest of Spain. A survey of the region was carried out, and the strains
isolated were identified as C. acutatum by using the polymerase chain react...
Phytopathogenic fungi are organisms responsible for several plant diseases in different crops around the world, causing very important economic losses to the farmers. Fungi have a complicated life cycle, normally with asexual and sexual reproduction that involves the formation of different reproductive structures. Moreover, during plant disease, fu...
Botrytis cinerea is a phytopathogenic fungus causing disease in a substantial number of economically important crops. In an attempt to identify putative fungal virulence factors, the two-dimensional gel electrophoresis (2-DE) protein profile from two B. cinerea strains differing in virulence and toxin production were compared. Protein extracts from...
Phytophthora spp. is one of the phytopathogenic Oomycete responsible for many important crop losses. Relevant species are P. infestans (causing potato late blight) and P. capsici (causing blight in pepper). In recent years, the use of conventional fungicides has favoured the appearance of different resistant strains. This study analyses the effect...
Botrytis cinerea is a phytopathogenic fungi causing disease in a number of important crops. It is considered a very complex species in which different populations seem to be adapted to different hosts. In order to characterize fungal virulence factors, a proteomic research was started. A protocol for protein extraction from mycelium tissue, with pr...
El cultivo de la vid es susceptible de sufrir numerosas enfermedades producidas por hongos filamentosos, las cuales ponen en serio peligro la producción de las cosechas y producen alteraciones en las características organolépticas del vino. Botrytis cinerea, causante de la enfermedad conocida como Podredumbre gris, es el hongo fitopatógeno más estu...
Botrytis cinerea is a pathogenic fungus that causes grey mould disease in a wide range of hosts, including grapevine (Vitis vinifera). Virulence of ten strains of the fungus was analysed through bioassays on detached leaves of two grapevine varieties growing in south-western Spain; one of them used for making Sherry wines (V. vinifera var. palomino...
Southwestern Spain is one of the main areas for strawberry culture in the world. Large losses are due to grey mould caused by Botrytis cinerea, a plant pathogen that affects a wide variety of crops. Resistance to synthetic fungicides is one of the more important problems that hamper control of grey mould on strawberry. We have characterized the rel...
The electrophoretic karyotype (EK) of 24 strains of Botryotinia fuckeliana was resolved to determine the extent of chromosomal polymorphism among them. Based on EK variation, nine different profiles with a number of bands ranging from 5 to 8 were found. Those profiles were not related to the origins of the strains, hosts, or year of isolation. Inhe...
Botrytis cinerea is a plant-pathogenic fungus that produces the disease known as grey mould in a wide variety of agriculturally important hosts in many countries. Ten strains from different locations collected on different years have been isolated and characterized by several methods (morphological, biochemical, genetical and molecular). Results sh...