María del Rocío Bustillos

• PhD
• Meritorious Autonomous University of Puebla

Many bacteria have the ability to survive in challenging environments; however, they cannot all grow on standard culture media, a phenomenon known as the viable but non-culturable (VBNC) state. Bacteria commonly enter the VBNC state under nutrient-poor environments or under stressful conditions. This review explores the concept of the VBNC state, p...

Many bacteria have the ability to survive in challenging environments; however, they cannot grow on standard culture media, a phenomenon known as the Viable but Non-Culturable (VBNC) state. Bacteria commonly go into the VBNC state under nutrient-poor environments or under stressful conditions. This review explores the concept of the VBNC state, pro...

Actividades de la APCM en 2022 y visibilidad de sus conferencias; un quehacer que tiene que ser reforzado Editado y revisado por: América Paulina Rivera-Urbalejo (Facultad de Estomatología, Benemérita Universidad Autónoma de Puebla, Puebla, México). Resumen La divulgación del conocimiento es imperante para el crecimiento de una sociedad y es uno de...

Rare-earth elements (REEs) are a group of metallic chemical elements that share some properties and that despite their name are not too rare in the Earth’s crust. Until recently, we did not know of any REEs that were necessary for any living organism. Now we know that there are some bacteria that use REEs to perform reactions that let them consume...

The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize (“Rojo Criollo”) and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production of antimicrobial compounds like 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin,...

In northern Mexico, aridity, salinity and high temperatures limit areas that can be cultivated. To investigate the nature of nitrogen-fixing symbionts of Phaseolus filiformis, an adapted wild bean species native to this region, their phylogenies were inferred by MLSA. Most rhizobia recovered belong to the proposed new species Ensifer aridi. Phyloge...

Munguía-Pérez R., ¿Es funcional la biorremediación en la eliminación de níquel en un suelo contaminado? Artículo Original Alianzas y Tendencias-BUAP. 2019, 4 (14): 30-xx. Recibido: 30 mayo 2019. Aceptado: 12 junio 2019. RESUMEN La contaminación por metales pesados representa un problema creciente en la cultura industrializada. Representan una amena...

Heavy metal accumulation in mesquite trees (Prosopis laevigata) growing in aluminum, titanium, chromium and zirconium-polluted soils of a semi-arid region in Mexico was investigated using wavelength dispersive X-ray fluorescence analysis. The results showed that P. laevigata trees can hyper accumulate up to 4100 mg/kg of Al, 14000 mg/kg of Fe, 1600...

La contaminación por metales pesados representa un problema creciente en la cultura industrializada. Representan una amenaza biológica, pues no son biodegradables, aunque existen mecanismos que poseen los organismos para acumular, traslocar, transformar, inmovilizar o estabilizarlos. El níquel es un metal pesado presente en descargas mineras, en la...

A random transposition mutant library of B. gladioli UAPS07070 was analyzed for searching mutants with impaired microbial antagonism. Three derivates showed diminished antimicrobial activity against a sensitive strain. The mutated loci showed high similarity to the quorum sensing genes of the AHL-synthase and its regulator. Another mutant was affec...

A random transposition mutant library of B. gladioli UAPS07070 was analyzed for searching mutants with impaired microbial antagonism. Three derivates showed diminished antimicrobial activity against a sensitive strain. The mutated loci showed high similarity to the quorum sensing genes of the AHL-synthase and its regulator. Another mutant was affec...

Plant growth-promoting rhizobacteria (PGPR) increase plant growth and crop productivity. The inoculation of plants with a bacterial mixture (consortium) apparently provides greater benefits to plant growth than inoculation with a single bacterial strain. In the present work, a bacterial consortium was formulated containing four compatible and desic...

Bacterial growth in SYP or PY-Ca liquid media for bacteria grown individually or all together. (PDF)

Physicochemical characteristics of the soils used for support in the desiccation experiments. (PDF)

Siderophore production of the four strains chosen for the bacterial consortium. (PDF)

Sequence identity of 16S rRNA from Acinetobacter sp. strain EMM02. (DOCX)

Number of bacteria present in suspensions used for the inoculation of maize. (DOCX)

Colonization of the rhizosphere of maize plants by the bacterial strains. (DOCX)

Flow chart illustrating the identification of Acinetobacter sp. EMM02 by the amplification and sequencing of the gene encoding 16S rRNA. (PDF)

Restriction patterns of the amplified gene encoding the 16S rRNA of the bacterial strains isolated from plants. (PDF)

Adhesion of strains to maize seeds sown in vermiculite. (DOCX)

Strains used in this study. (DOCX)

We describe a method to evaluate bacterial tolerance to air desiccation at 30 °C and 50% relative humidity.

Here we describe two methods for assessing the ability of one bacteria to inhibit the growth of another through the production of antimicrobial compounds. In the first assay, the double agar layer method, only the secreted substances but not the candidate antagonist bacteria interact with the sensitive microorganisms. In the second assay, the simul...

Methanol-consuming culturable bacteria were isolated from the plant surface, rhizosphere, and inside the stem of Neobuxbaumia macrocephala. All 38 isolates were facultative methylotrophic microorganisms. Their classification included the Classes Actinobacteria, Sphingobacteriia, Alpha-, Beta-, and Gammaproteobacteria. The deduced amino acid sequenc...

Mexican food is made with various natural products. The objective of the present work was to make a reflection on the natural components used to prepare the typical Mexican food and to make known which of them contain compounds with antimicrobial capacity. It was noticed that many of the natural ingredients of the typical Mexican food have compound...

The present invention contemplates the development and application of a multi- species inoculant formulation consisting of the following beneficial bacterial species: (1) Microbacterium sp. UAPS01200 (RhPf3); (2) Microbacterium sp. UAPS01203 (4MePi8) and (3) Pseudomonas putida KT2440; to improve growth of plants in semi-desert zones such as corn an...

Among the dominant woody legumes in arid and semiarid regions throughout the world are the 45 species of Prosopis. The woody legume mesquite is well adapted to warm and dry tropical climates. It grows well in areas receiving 250-600 mm annual rainfall. It is a fast growing tree and has a deep to very deep, well meshed root system. It is capable of...

Among the dominant woody legumes in arid and semiarid regions throughout the world are the 45 species of Prosopis. The woody legume mesquite is well adapted to warm and dry tropical climates. It grows well in areas receiving 250-600 mm annual rainfall. It is a fast growing tree and has a deep to very deep, well meshed root system. It is capable of...

Several rhizospheric bacteria with potential to stimulate the plant growth have been isolated from different places and regions of the world. The mechanisms by which rhizospheric bacteria have the ability to stimulate plant growth include phytohormone production, volatiles compounds, antimicrobial compounds (lytic enzymes production, siderophores),...

Advances in science have opened up new options for farmers responding to market needs and environmental challenges. These techniques are included in what is often referred to as “biotechnology”. Appropriate use of science and technology helps to meet agricultural challenges and consumer needs of the 21st century. Farmers have been improving wild pl...

130 Abstract: An alternative for increasing the yield of crops is the use of PGPR, for this purpose the colonization is a critical process. In this work the chemotaxis toward tomato plants and bacteria colonization were evaluated for P. putida KT2440 and mutant strains in specific chemoreceptors for GABA and succinato. Axenic seedlings of three day...

Echinocactus platyacanthus es una cactácea globosa, endémica de México, forma parte de la flora de la Reserva de la Biósfera "Tehuacán-Cuicatlán". Actualmente este cactus se encuentra amenazado debido a sus diversos usos, entre los cuales destaca la elaboración de un dulce. El crecimiento de la planta es muy lento, puede llegar a alcanzar una edad...

Azotobacter vinelandii is a soil bacterium that produces the polysaccharide alginate. The two-component system GacS/GacA is required for alginate synthesis since a mutation in gacS or gacA significantly reduced the level of transcripts of algD, the gene encoding GDP-mannose dehydrogenase, a key enzyme of the alginate biosynthetic pathway. In many γ...

Pink disease is a major problem in the pineapple canning industry. Affected fruit acquire a brownish pigment after pasteurization and can contaminate non-affected fruit before they are released to the consumer. In the last few years, Pantoea citrea has been described as the causative agent of pink disease. In this study, over 300 bacterial isolates...

Bacterial isolates from maize plants showing a leaf spot disease were identified through molecular and phenotypic traits, showing that the isolates belong to Pantoea ananatis. Maize plants inoculated with those isolates showed a pathogenic reaction. This is the first report of a disease of Mexican maize caused by P. ananatis. Additional keywordsEn...

La asociación planta-bacteria es esencial para la supervivencia de esta última durante la desecación, sin embargo, no todos los exudados de la planta son útiles para la resistencia bacteriana. Será interesante determinar la presencia y concentración de disacáridos, monosacáridos y polialcoholes en exudados de plantas de maíz y correlacionarlos con...

Rhizosphere and endophytic Azospirillum brasilense isolates recovered from sugarcane plants and the reference strains Sp7 and Cd were analyzed for plasmid occurrence. All of the 26 A. brasilense isolates analyzed harbored from five to eight replicons. Several strains contained small plasmids from 45 to 70 kb, but all of the isolates harbored other...

The presence of endophytic Acetobacter diazotrophicus was tested for pineapple plants (Ananas comosus [L.] Merr.) grown in the field. Diazotrophic bacteria were isolated from the inner tissues of surface sterilized roots, stems, and leaves of pineapple plants. Phenotypic tests permitted the selection of presumptive nitrogen-fixing A. diazotrophicus...