Maria Beatrice Bitonti

Maria Beatrice Bitonti
Università della Calabria | Università della Calabria · Department of Biology, Ecology and Earth Science - DiBEST

About

120
Publications
25,550
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,352
Citations
Citations since 2017
20 Research Items
935 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150

Publications

Publications (120)
Article
Peaches (Prunus persica L. Batsch) are appreciated for their distinctive aroma, and e.g. sweetness, color, and texture, which vary across different cultivars. Peaches are produced commercially in warmer temperate regions such as southern Europe, and exported to northern Europe. But being perishable fruits, they are transported cooled to delay ripen...
Article
Full-text available
Introduction Peach ( Prunus persica (L.) Batsch,) and nectarine fruits (Prunus persica (L.) Batsch, var nectarine), are characterized by a rapid deterioration at room temperature. Therefore, cold storage is widely used to delay fruit post-harvest ripening and extend fruit commercial life. Physiological disorders, collectively known as chilling inju...
Article
Full-text available
Cold storage is used to extend peach commercial life, but can affect quality. Quality changes are assessed through the content of nutritionally relevant compounds, aroma, physical characters and/or sensorially. Here, six peach and nectarine cultivars were sampled at commercial harvest and after 7 days of 1 °C storage. A trained panel was used to ev...
Article
Full-text available
Nectarines are perishable fruits grown in Southern Europe, valued for their sensorial properties. Chilling is used in the supply chain for Northern European consumers, while Southern European consumers can access fresh, locally grown fruit or cold-stored supermarket fruit. Cold storage and fruit ripening affect texture and flavour. Here a consumer...
Article
The ddc mutant of Arabidopsis thaliana is characterized by pleiotropic phenotypic alterations including a curl-shaped leaf, previously explained by disturbed auxin metabolism and transport. The present study was aimed at further explore the molecular bases underlying the abnormal phenotype of the ddc leaf. We demonstrated that genes specifically re...
Article
Full-text available
Background: There is increasing consumer demand for traceability of olive oil. However, genotype, environmental factors and stage of maturity, all affect both the flavour and composition of both the olives and olive oil. Few studies have included all three variables. Key metabolites include lipids, phenolics and a wide range of volatile organic co...
Article
Full-text available
DNA methylation plays an important role in modulating plant growth plasticity in response to stress, but mechanisms involved in such control need further investigation. We used drm1 drm2 cmt3 mutant of Arabidopsis thaliana, defective in DNA methylation, to explore metabolic pathways downstream epigenetic modulation under cadmium (Cd) stress. To thi...
Article
Full-text available
Abstract Peaches have a short shelf life and require chilling during storage and transport. Peach aroma is important for consumer preference and determined by underlying metabolic pathways and gene expression. Differences in aroma (profiles of volatile organic compounds, VOCs) have been widely reported across cultivars and in response to cold stora...
Article
Full-text available
Cynara cardunculus subsp. cardunculus, belonging to Asteraceae family, and widely distributed in the Mediterranean area, exhibits high nutraceutical and nutritional potential, as compared to the cultivated varieties. This work reports, for the first time, on genetic and metabolic characteristics of three natural populations of C. cardunculus presen...
Article
Full-text available
Background: Olive (Olea europaea L.) is an emblematic oil tree crop in the Mediterranean basin. Currently, despite olive features as a moderately thermophilic species, its cultivation is worldwide spreading due to the health-related impact of olive products on human nutrition. A point of concern for the expanding olive cultivation is related to th...
Article
Full-text available
Background: The ripening process of olive fruits is associated with chemical and/or enzymatic specific transformations, making them particularly attractive to animals and humans. In olive drupes, including 'Cassanese' ones, ripening is usually accompanied by progressive chromatic change, resulting in a final red-brown colourization of both epiderm...
Article
Full-text available
WEE1 regulates the cell cycle by inactivating cyclin dependent protein kinases (CDKs) via phosphorylation. In yeast and animal cells, CDC25 phosphatase dephosphorylates the CDK releasing cells into mitosis, but in plants, its role is less clear. Expression of fission yeast CDC25 (Spcdc25) in tobacco results in small cell size, premature flowering a...
Article
In this investigation, we assessed the effects of Cu and/or Cd excess on physiological and metabolic processes of the widespread seagrass Zostera marina. Adult were exposed to low Cd and Cu (0.89 and 0.8 µM, respectively) and high Cd and Cu (8.9 and 2.4 µM, respectively) for 6 d at: Control conditions; low Cu; high Cu; low Cd; high Cd; low Cd and l...
Article
Farnesene is a sesquiterpene with semiochemical activity involved in interspecies communication. This molecule, known for its phytotoxic potential and its effects on root morphology and anatomy, caused anisotropic growth, bold roots and a "left-handedness" phenotype. These clues suggested an alteration of auxin distribution, and for this reason, th...
Article
Full-text available
Cadmium is one of the most widespread pollutant in both terrestrial and marine environment, and its inhibitory effect on plant growth has been largely demonstrated. However, the molecular mechanisms underlying Cd toxicity in plant and mainly in root, as the first organ sensing soil heavy metals, need to be better investigated. To this aim, in the p...
Article
Full-text available
A new highly sensitive whole-mount in situ hybridization method, based on tyramide signal amplification (TSA-MISH) was developed and a combined GFP detection and TSA-MISH procedure was applied for the first time in plants, to precisely define the spatial pattern of AtGUS1 and AtGUS2 expression in the root apex. β-glucuronidases (GUSs) belonging to...
Article
Full-text available
Dehydrins belong to a protein family whose expression may be induced or enhanced by developmental process and environmental stresses that lead to cell dehydration. A dehydrin gene named OesDHN was isolated and characterized from oleaster (Olea europaea L. subsp. europaea, var. sylvestris), the wild form of olive. To elucidate the contribution of Oe...
Article
Previous investigations have revealed that Ectocarpus siliculosus strain Es524, isolated from a highly copper (Cu)-polluted location in Chile, displays higher activities of the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) under Cu excess. This partly explains its enhanced tolerance compared with othe...
Article
The KNOTTED-like transcription factors (KNOX) contribute to plant organ development. The expression patterns of peach KNOX genes showed that the class 1 members act precociously (S1-S2 stages) and differentially during drupe growth. Specifically, the transcription of KNOPE1 and 6 decreased from early (cell division) to late (cell expansion) S1 sub-...
Article
Full-text available
Xyloglucan oligosaccharides (XGOs) are breakdown products of XGs, the most abundant hemicelluloses of the primary cell walls of non-Poalean species. Treatment of cell cultures or whole plants with XGOs results in accelerated cell elongation and cell division, changes in primary root growth, and a stimulation of defence responses. They may therefore...
Article
Germins and germin-like proteins (GLPs) constitute an ubiquitous family of plant proteins that seem to be involved in many developmental and stress related processes. A novel GLP cDNA was isolated from Citrus limon and structural features and genomic organization were investigated by in silico and Southern blots analysis. In lemon, the ClGLP1 encod...
Article
Full-text available
The brown seaweed Ectocarpus siliculosus is an emerging model species distributed worldwide in temperate coastal ecosystems. Over 1500 strains of E. siliculosus are available in culture from a broad range of geographic locations and ecological niches. To elucidate the molecular mechanisms underlying its capacity to cope with different environmental...
Article
Full-text available
The nuclear histone content has been estimated in the 2C (DNA- presynthesis phase) meristematic nuclei of Triticum durum embryo from the fertilization to the ripening time. The apparison of histone heterogeneity and its clear increase during the last step of embryo morphogenesis compelled us to suppose that a certain amount and/or quality of histon...
Article
Full-text available
The duration of the mitotic cycle and its phases has been determined in the root tips of seedlings from brown and black caryopses of Haynaldia villosa, by autoradiographic technique after labelling with m3H-thymidine. The total duration of the mitotic cycle was found to be 12h in brown caryopses and 9.5h in black caryopses. As to the time length of...
Article
Full-text available
The nuclei of the first node embryo of Triticum durum show the same amount of histone bound to DNA in young and in aged seeds in line with earlier findings of remaining metabolic activity. Furthermore the explants of the first node from the old seeds analyzed, are still able to generate calli and plantlets and might be usefull to recover embryos fr...
Article
Full-text available
Genomic changes in Pinus laricio were observed affecting a terminal bud which stops its growth thereby stimulating supernumerary lateral bud production. The changes which occur in the bud meristem results in a compact branch able to produce small ovulate cones, but it never produces pollen cones. Nevertheless sexual reproduction occurs and this gen...
Article
Full-text available
Secale cereale produces, in the same ear, brown and black caryopses, which differ widely in size and morphology. These phenotypic differences are correlated to different variations in both germination and viability during seeds lifespan. Spontaneous mutagenesis analysis demonstrates that the two kinds of seeds display a different nuclear damage dur...
Article
Full-text available
A more abundant production of lateral roots in Vicia faba seedlings was observed after treatment with ascorbic acid, an effect which was primarily due to enhanced pericycle cells activity. The histochemical localization, as revealed by the reduction of silver nitrate into metallic silver, demonstrated that a wide stain intensity is detectable in th...
Article
Full-text available
The histone/DNA ratio has been estimated independently for each embryonic area in young and old caryopses of Triticum durum. As the ratio is already quite different in the single embryo areas of over dormant young seeds, it can be suggested that nuclear conditions at ripening time influence the response of the cell populations which undergo senesce...
Article
Full-text available
Columnar epithelium and parenchyma cells of the scutellum of Triticum durum caryopses show different quiescence models: while epithelium cells display a DNA content per nucleus of 2C, the parenchyma cells undergo poli- ploidization. Furthermore, in old seeds, the distribution of the histones/DNA ratio shifts much more in parenchyma cells than in co...
Article
Full-text available
The histones/DNA ratio has been calculated in the 2C nuclei from the primary root meristems of young and old caryopses of Triticum durum. In a first experiment young and old caryopses taken from different crops were considered in one and the same trial, at the same time. In a second experiment young and old caryopses taken from the very same crop w...
Article
Full-text available
The pericycle cells in Allium cepa root tip undergo differentiation at 2C level. The present study indicates two clear effects of ascorbic acid treatment on pericycle cell line: i) the extension of the upper limit of mitosis from the tip; ii) the increase of the labelling index. In a previous paper we have just found that, in Allium cepa, treatment...
Article
Full-text available
Olea europaea L. subsp. europaea var. sylvestris, also named oleaster, is the wild form of olive and it is used as rootstock and pollen donor for many cultivated varieties. An efficient procedure for in vitro propagation of oleaster was established in this study. A zeatin concentration of 2.5 mg/L was effective to induce an appreciable vegetative g...
Data
Full-text available
In yeasts and animals, premature entry into mitosis is prevented by the inhibitory phosphorylation of cyclin-dependent kinase (CDK) by WEE1 kinase, and, at mitosis, WEE1 protein is removed through the action of the 26S proteasome. Although in higher plants WEE1 function has been confirmed in the DNA replication checkpoint, Arabidopsis wee1 insertio...
Article
Full-text available
In yeasts and animals, premature entry into mitosis is prevented by the inhibitory phosphorylation of cyclin-dependent kinase (CDK) by WEE1 kinase, and, at mitosis, WEE1 protein is removed through the action of the 26S proteasome. Although in higher plants WEE1 function has been confirmed in the DNA replication checkpoint, Arabidopsis wee1 insertio...
Article
Full-text available
ABSTRACT: Growth and distribution of seagrasses is closely related to undersea light quality and intensity. Currently, several factors linked to anthropogenic pressure, such as increased sediment runoff, resuspension of bottom sediments, and algae accumulations, reduce sub-surface light availability in most Mediterranean coastal areas, and are caus...
Article
Full-text available
Background and AimsHow plant cell-cycle genes interface with development is unclear. Preliminary evidence from our laboratory suggested that over-expression of the cell cycle checkpoint gene, WEE1, repressed growth and development. Here the hypothesis is tested that the level of WEE1 has a dosage effect on growth and development in Arabidospis thal...
Article
Full-text available
The KNOTTED-like (KNOX) genes encode homeodomain transcription factors and regulate several processes of plant organ development. The peach (Prunus persica L. Batsch) genome was found to contain 10 KNOX members (KNOPE genes); six of them were experimentally located on the Prunus reference map and the class 1 KNOPE1 was found to link to a quantitati...
Article
Full-text available
Silene nicaeensis is a wild Mediterranean grass often restricted to sandy sea shore and exhibiting an excellent tolerance to drought and salinity. Within Silene genus, several heavy metal-tolerant ecotypes have been identified, but information on molecular basis of such metal tolerance is still limited. Conceivably, salt-tolerant plants may represe...
Article
Full-text available
Entry into mitosis is regulated by cyclin dependent kinases that in turn are phosphoregulated. In most eukaryotes, phosphoregulation is through WEE1 kinase and CDC25 phosphatase. In higher plants a homologous CDC25 gene is unconfirmed and hence the mitotic inducer Schizosaccharomyces pombe (Sp) cdc25 has been used as a tool in transgenic plants to...
Data
Figure S2. Null effect of Spcdc25 expression on cell length in mature regions of primary root. DIC/Nomarski images of regions of primary roots exhibiting young lateral root primordia (YLRP) in WT and Spcdc25. Mean (± SE) cortical cell length. WT = 75.14 ± 10.10 Spcdc25 = 56.86 ± 11.61 μm. n = 25. Bar scale = 40 μm.
Data
Table showing genes up-or down-regulated by 2-fold or more (Spcdc25/WT).
Data
Expression levels of Spcdc25 in transgenic lines of Arabidopsis. RT-PCR of BTX::Spcdc25 lines 9 and 10 showing expression of the transgene in both lines (lower band is primer dimer).
Data
Expression of Spcdc25 induces increased rooting in cultured hypocotyls at NAA/Kin: 50/100, 50/200, 25/50, 25/200. Hypocotyls of 14 d old plants of (A) wild type (WT) (B) BTX::Spcdc25 (line 9) cultured on two-way concentration gradients of increasing concentrations (ng ml-1) of naphthyl acetic acid (NAA) and kinetin (Kin) for 30 d; representative gr...
Data
Real time PCR verification of the microarray results. Above each histogram bar (Spcdc25/WT) is the microarray result (Spcdc25/WT). n = 3.
Article
Full-text available
In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylation level and pattern were analysed in actively grow...
Article
Full-text available
Aside from those on Arabidopsis, very few studies have focused on spatial expression of cyclin-dependent kinases (CDKs) in root apical meristems (RAMs), and, indeed, none has been undertaken for open meristems. The extent of interfacing between cell cycle genes and plant growth regulators is also an increasingly important issue in plant cell cycle...
Article
Full-text available
In yeasts and animals, cyclin-dependent kinases are key regulators of cell cycle progression and are negatively and positively regulated by WEE1 kinase and CDC25 phosphatase, respectively. In higher plants a full-length orthologue of CDC25 has not been isolated but a shorter gene with homology only to the C-terminal catalytic domain is present. The...
Article
Full-text available
In situ RNA-RNA hybridization (ISH) is a molecular method for localization of gene transcripts at the cellular level and is widely used to provide spatial and temporal information regarding gene expression. However, standard protocols are complex and laborious to implement, restricting analysis to one or a few genes at any one time, each one observ...
Chapter
Full-text available
In higher plants, growth and development relies on the spatiotemporal regulation of gene expression, which is under the control of both endogenous signals and external stimuli. In this chapter, recent advances in defining signaling machinery and genetic frameworks that underlie RAM patterning and maintenance are reviewed, with a focus on the interp...
Article
Full-text available
In past decades, meadows of Posidonia oceanica (L) Delile have declined extensively in the Mediterranean basin mainly due to disturbances associated with reduced light availability. To investigate the molecular mechanisms of photoacclimation in this seagrass, we selected the CHL P gene as a marker of plant response to light availability because it...
Article
Full-text available
Current and predicted climate changes caused by global warming compel greater understanding of the molecular mechanisms that plants use to survive drought. The desiccation-tolerant fern Polypodium polypodioides exhibits extensive cell wall folding when dried to less than 15% relative water content (RWC) and rapidly (within 24 h) rehydrates when exp...