Mari F. C. M. van den Hout

Mari F. C. M. van den Hout
Maastricht Universitair Medisch Centrum · Pathology

Doctor of Medicine - PhD

About

32
Publications
16,643
Reads
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474
Citations
Additional affiliations
April 2016 - present
Maastricht Universitair Medisch Centrum
Position
  • Pathologist
January 2016 - March 2016
Massachusetts General Hospital
Position
  • visiting fellow
December 2010 - March 2016
Leiden University Medical Centre
Position
  • Medical Doctor
Education
August 1998 - July 2005
KU Leuven
Field of study
  • Medicine

Publications

Publications (32)
Article
Full-text available
Radical resection for patients with oral cavity cancer remains challenging. Rapid evaporative ionization mass spectrometry (REIMS) of electrosurgical vapors has been reported for real-time classification of normal and tumor tissues for numerous surgical applications. However, the infiltrative pattern of invasion of oral squamous cell carcinomas (OS...
Article
Full-text available
This study aimed to assess the prognostic value of intratumoral CD57+ cells in head and neck squamous cell carcinoma (HNSCC) and to examine the reproducibility of these analyses using QuPath. Pretreatment biopsies of 159 patients with HPV-negative, stage III/IV HNSCC treated with chemoradiotherapy were immunohistochemically stained for CD57. The nu...
Article
Full-text available
PURPOSE Under common therapeutic regimens, the prognosis of human papillomavirus (HPV)–positive squamous oropharyngeal carcinomas (OPCs) is more favorable than HPV-negative OPCs. However, the prognosis of some tumors is dismal, and validated prognostic factors are missing in clinical practice. The present work aimed to validate the prognostic signi...
Article
Full-text available
Objective: To describe a case of complete spontaneous regression of a middle ear melanoma. Patient: We present a case of a 68-year-old man with complaints of unilateral hearing loss and an ipsilateral facial nerve paresis. Radiological and histopathological examination revealed a cT4bN0M0 mucosal melanoma of the middle ear. Interventions: The...
Article
Full-text available
Sclerosing polycystic adenoma (SPA) is a rare salivary gland neoplasm originally thought to represent a non-neoplastic lesion. Recently we have encountered an index case of apocrine intraductal carcinoma of parotid gland of 62-year-old man with invasive salivary duct carcinoma component arising from SPA, a combination of tumor entities that has nev...
Article
Full-text available
YAP1-TFE3-fused hemangioendothelioma is an extremely rare malignant vascular tumor. We present the largest multi-institutional clinicopathologic study of YAP1-TFE3-fused hemangioendothelioma to date. The 24 cases of YAP1-TFE3-fused hemangioendothelioma showed a female predominance (17 female, 7 male) across a wide age range (20-78 years old, median...
Article
Full-text available
Background We previously reported CpG-B injection at the primary tumor excision site prior to re-excision and sentinel node biopsy to result in immune activation of the sentinel lymph node (SLN), increased melanoma-specific CD8 ⁺ T cell rates in peripheral blood, and prolonged recurrence-free survival. Here, we assessed recruitment and activation o...
Article
Full-text available
Real-time tissue classifiers based on molecular patterns are emerging tools for fast tumor diagnosis. Here, we used rapid evaporative ionization mass spectrometry (REIMS) and multivariate statistical analysis (principal component analysis–linear discriminant analysis) to classify tissues with subsequent comparison to gold standard histopathology. W...
Article
Full-text available
Objective Determine the presence and prognostic value of human papillomavirus (HPV), Epstein-Barr virus (EBV), Merkel cell polyomavirus (MCPyV), and cell cycle proteins in head and neck squamous cell carcinoma (HNSCC) of non-smokers and non-drinkers (NSND). Methods Clinical characteristics and tumors of 119 NSND with HNSCC were retrospectively col...
Article
Full-text available
Expression of programmed cell death-ligand 1 (PD-L1) is being used as predictive biomarker for immunotherapy in head and neck squamous cell carcinoma (HNSCC). Several antibodies are available for PD-L1 testing and multiple staining and scoring methods are used. This study aimed to compare the performance of two PD-L1 standardized assays (SP263 and...
Article
Full-text available
Primary rhabdomyosarcoma (RMS) of the adult prostate is a very rare tumor with only 45 cases published to date. The clinical course of RMS of the prostate is very aggressive, and prognosis is very poor. Here we describe two cases of primary RMS of the prostate of adult patients and discuss the differential diagnosis of RMS with other mesenchymal tu...
Poster
e18538 Background: The local recurrence rate of oral squamous cell carcinomas (OSCC) hardly decreases despite the best possible treatment. Partly, this is caused by the presence of (pre)malignant cells in the surrounding tissue, which can develop into a new malignancy in time. Histological recognition of these cells in resection margins appears to...
Article
Full-text available
Whereas TLR9 agonists are recognized as powerful stimulators of antitumor immunity, GM-CSF has had mixed reviews. In previously reported randomized trials we assessed the effects of local immune modulation in early-stage melanoma with CpG-B alone or with GM-CSF. Here we discuss the added value of GM-CSF and show sex-related differences.
Poster
Full-text available
The local recurrence rate of oral SCCs (OSCCs) hardly decreases. This is partly due to the presence of (pre)malignant cells in the remaining tissue after resection, which may lead to the development of a new tumour in time. Because histopathologic detection of (pre)malignant cells in tumour resection margins is unreliable to recognize patients at r...
Article
Full-text available
p> Purpose: Although risk of recurrence after surgical removal of clinical stage I–II melanoma is considerable, there is no adjuvant therapy with proven efficacy. Here, we provide clinical evidence that a local conditioning regimen, aimed at immunologic arming of the tumor-draining lymph nodes, may provide durable protection against disease recurre...
Article
Full-text available
Melanoma exerts immune suppressive effects to facilitate tumor progression and metastatic spread. We studied these effects on dendritic cell (DC) and T-cell subsets in 36 melanoma sentinel lymph nodes (SLN) from 28 stage I-III melanoma patients and determined their clinical significance. Four conventional DC subsets; plasmacytoid DCs; and CD4+, CD8...
Article
Introduction: Currently, there is no widely used adjuvant treatment available to improve survival after surgical excision of localized melanoma. Here, we present the clinical outcome of patients who participated in three randomized phase-II trials and received low-dose local immunotherapy, which was shown to be safe and to boost loco-regional and s...
Article
Full-text available
Impaired immune effector functions in the melanoma sentinel lymph node (SLN) may allow for early metastatic events. In an effort to determine the optimal way to strengthen immune defenses, 28 clinical stage I–II melanoma patients were randomized in a 3-arm Phase II study to receive, prior to excision and sampling of the SLN, i.d. injections of sali...
Article
Although 5-year overall survival rates in patients with Stage I-II melanoma range from 95% to as low as 45%, currently there is no adjuvant treatment available that has been shown to reduce the chances of recurrence after surgical excision of localized melanoma. Between 2004 and 2007 we conducted two randomized and placebo controlled phase II clini...
Article
Full-text available
Pathologic staging of oncologic specimens includes the identification of the accurate lymph node status. Retrieving more lymph nodes leads to a more reliable N0 status in the TNM classification. The aim of this prospective study was to evaluate whether more lymph nodes can be retrieved from oncologic resection specimens when more time is invested i...
Article
Full-text available
Melanoma-induced suppression of Dendritic Cells (DC) in the Sentinel Lymph Node (SLN) interferes with the generation of protective anti-tumor immunity. In an effort to strengthen immune defenses against metastatic spread, 28 Stage I-III melanoma patients were randomized in a 3-arm Phase-II study to receive i.d. injections around the primary tumor e...
Conference Paper
Full-text available
Melanoma cutis is virtually incurable when it has metastasized to distant organs. Unfortunately, there is no safe and effective adjuvant treatment available for early stage patients that may prevent the development of metastasis. Since early melanoma development is accompanied by impaired immune effector functions in the sentinel lymph node (SLN),...
Article
Full-text available
The exact role of Langerhans cells (LCs) in the induction and regulation of T-cell immunity is an important focus of research with possible implications for the immunotherapy of cancer.[1][1][⇓][2]–[3][3] Recently we reported on the phenotypic and functional characterization of dendritic cell (
Article
Full-text available
To increase (tumor) vaccine efficacy, there is an urgent need for phenotypic and functional characterization of human dendritic cell (DC) subsets residing in lymphoid tissues. In this study we identified and functionally tested 4 human conventional DC (cDC) subsets within skin-draining sentinel lymph nodes (SLNs) from early-stage melanoma patients....
Article
Full-text available
Prognosis in patients with sentinel node (SN)-positive melanoma correlates with several characteristics of the metastases in the SN such as size and site. These factors reflect biologic behavior and may separate out patients who may or may not need additional locoregional and/or systemic therapy. Between 1993 and 2008, 1,080 patients (509 women and...
Article
Full-text available
We have been studying the re-activation of tumor-associated antigen (TAA)-specific CD8(+) T cells in sentinel lymph nodes (SLN) of melanoma patients upon intradermal administration of the CpG-B oligodeoxynucleotide PF-3512676. To facilitate functional testing of T cells from small SLN samples, high-efficiency polyclonal T cell expansion is required...
Article
The sentinel lymph node (SLN) procedure has proven a useful prognostic tool for the assessment of melanoma relapse and mortality risk, but is also of great value for the assessment of immunological interventions. Since early melanoma development is accompanied by a suppressed immune state of the SLN, there is a strong rationale for therapeutic immu...

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