Margarita Gabrashanska

Bulgarian Academy of Sciences | BAS · Parasitology

The effect of dietary manganese from two different sources on chicks (uninfected and infected with Ascaridia galli ) was studied. Chick diet was supplemented with 0.9 g Mn ²⁺ kg ⁻¹ food either in the form of MnSO 4 .H 2 O or 2Gly. MnCl 2 .2H 2 O for 20 days. Chicks were divided into six groups: group 0, control; group 1, control + MnSO 4 .H 2 O; gr...

The action of mixed salts of copper and zinc (basic and neutral) on Hisex chickens experimentally infected with Ascaridia galli has been studied. The data show that the lowest host mortality and decrease in body weight gain and the highest reduction in nematode loading occurs in infected chickens treated with basic salts (in comparison with infecte...

Background Coal and coal processing industries provoke trace metal pollution, which has a negative effect on the water – soil – vegetation - small mamals eco-system, constituting part of the food chain and exerting a serious impact on human health. Objectives Assessment of the environmental impact of Maritza iztok coal complex, situated east of Ra...

The aim of our study was to investigate parameters of oxidative/antioxidative status in rats experimentally infected with Fasciola hepatica and treated with a copper salt. The experiment was carried out on 24 male Wistar albino rats, divided into 4 groups with 6 animals in each: group 1 – healthy untreated animals, group 2 – rats orally infected wi...

Rat intestinal parasites have attracted great interest as bioindicators for environmental quality since they can bioconcentrate several heavy metals to much higher concentrations than the tissues of the hosts. Our aim was to assess the potential of the cestode Hymenolepis spp. as a bioindicator for heavy metal pollution in an industrial area of the...

Background: The impact of chemical elements on the biosphere is a function of their concentration and chemical form. Elucidation and prognosing of the latters in water basins and soil extracts is of particular significance for the assessment of their bioaccumulation in plants and animals. Objectives: Trace metals dynamics in the system water - s...

Polyzwitterions (PZI) recently emerged as biomaterials with excellent bio- and haemo-compatibility, demonstrating lower protein adsorption on their surfaces even compared to the golden standard in the field – poly(ethylene glycol) (PEG). Although PZI combine many beneficial for chronic wound treatment properties as nonfouling ability and high capac...

Antiproliferative effects of biochemically isolated from Trichinella spiralis muscle larvae, livers and spleens from healthy and T. spiralis infected rats heat-sensitive bioactive substances (HSBSes) were studied in vitro on nonactivated and mitogen activated with phytohemagglutinin (PHA) lymphocytes and bone marrow precursor cells. Stronger antipr...

The current study was carried out to study the impact of disparate geographical regions on genetic structures variability of Haemonchus species populations among small ruminants from Egypt and Bulgaria using mtDNA markers and to diagnose the predominant Haemonchus species isolated from the naturally infected sheep and goats through studying the gen...

The structural, functional and metabolic changes in host tissues caused during penetration, migration and localization of helminths in the host body, are associated with specific changes in trace element levels in body tissues, including these of liver and spleen. The aim of the present study is determination of the quantities of trace elements zin...

Calcium-phosphate (Ca-P) cements are widely used as bone substitutes in orthopedic, reconstructive and maxillofacial surgery because they have good biocompatibility and extensive bone conductivity. Many bivalent trace metallic ions have demonstrated their beneficial effects in bones tissues engineering applications. A bone-related enzyme alkaline p...

Application and development of biocompatible zwitterionic polymers and composite polymer-reinforced calcium phosphate pastes and cements in combination with specific drugs, have been considered as a promising strategy in biomedicine. In bone tissue engineering and dental medicine with beneficial effects are used calcium-phosphate (Ca-P) cements and...

Hydrogels are polymeric materials distinguished by high water content and diverse physical and chemical properties. Polymer hydrogels are widely used as scaffolds and materials for biomedical application due to the close mechanical behavior that they possess as compared to the body tissues. The aim of our study was to investigate the biocompatibil...

40 delivery has been documented for decades, some questions regarding the CD/drug interactions still wait to be answered in the quest for more operative and precise CD-based drug delivery systems. CD/drug interactions are quite intriguing when CD serves as an excipient for drug formulation of Pt-based anticancer drugs. Aim of our study is to elucid...

The application of zinc as a compound of 2Gly.ZnCI2.2H20 improves growth, normalize antioxidant and zinc status in the infected with Trichinella mice and reduce the number of Trichinella muscle larvae without development or manifestation of toxicity. In such a context the use of the highly bioavailable 2Gly.ZnCI2.2H20 helps to increase the security...

The current study was carried out to assess in vitro and in vivo effects of Moringa oleifera seed methanolic extract on Fasciola hepatica to develop an alternative source of treatment. The in vitro ovicidal effect of M. oleifera seed extract on immature F. hepatica eggs has provided evidence of inhibitory activity on the vitality and hatchability o...

This study was aimed to evaluate content of heavy metals in Lepus europeas Pallas 1778 and its helminth Trichuris sylvilagi (Nematoda) in Kardzhali region, Bulgaria. The contents of Cd, Pb, Zn, Mo, Mn, and Co were determined in liver, kidney, muscle and helminths. A metal bioaccumulation factors were calculated. Significant differences in the conce...

The contents of spleen trace elements copper (Cu), zinc (Zn), iron (Fe), and cobalt (Co) were determined after basic Cu-Zn salt application in rats with experimental chronic fasciolosis. The Cu-Zn salt treatment of healthy animals caused statistically significant increased quantity of spleen Zn and nearly unchanged levels of Co, Cu and Fe compared...

Contents of spleen trace elements copper (Cu), zinc (Zn), iron (Fe), and cobalt (Co) were investigated after mixed Cu-Zn basic salt treatment in rats with acute fasciolosis. The application of Cu-Zn salt on healthy rats caused increasing of spleen Zn level. Significant decreasing of spleen Fe quantity and slight decreasing of Zn and Cu levels were...

The present study was carried out for evaluation of some blood biochemical markers in rats with artificially created calvarial defects, implanted with modified calcium phosphates cements. Cements were synthesized on the bases of TTCP/DCPA, carboxylic acids, xanthan gum and glycerin. Rat blood biochemical parameters alanine aminotransferase (ALT), a...

Haemonchus contortus (H. contortus) remains important nematode that infecting sheep all over the world. Truthful diagnosis of haemonchosis needs reliable Enzyme linked immune sorbent assay test as well as the immuno-reactive protein profile of different prepared H. contortus antigens; larval (L), excretory secretory product (ESP) and adult somatic...

Abstract The present study aims to examine the status of trace elements copper (Cu), zinc (Zn), manganese (Mn), iron (Fe) and cobalt (Co) in the livers of rats with acute fasciolosis after treatment with mixed Zn-Cu basic salt {Zn5-xCux(OH)8Cl2.H20}. After the treatment of the experimental animals with Zn-Cu basic salt, the quantities of Cu and Zn...

The present study aims to examine the status of trace elements copper (Cu), zinc (Zn), manganese (Mn), iron (Fe) and cobalt (Co) in the livers of rats with acute fasciolosis after treatment with mixed Zn-Cu basic salt {Zn5-xCux(OH)8Cl2.H20}. After the treatment of the experimental animals with Zn-Cu basic salt, the quantities of Cu and Zn are signi...

The contents of toxic elements molybdenum (Mo), arsenic (As) and strontium (Sr) were investigated in mature nematodes Haemonchus contortus and in livers, kidneys and muscles of healthy and experimentally infected with H. contortus lambs. We found that the concentrations of these toxic components were significantly lower in the helminthes than in th...

This study aimed to evaluate the effects of intestinal Eimeria spp. on the levels of trace elements (zinc, copper, selenium and iron) in the serum and liver of rabbits naturally infected with Eimeria spp. The results showed that blood Se, Fe and Zn concentrations were decreased and Cu concentration was increased. The levels of Zn, Fe and Se were in...

The aim of the present study was to investigate the trace element content in the liver tissue after the combined effect of chronic Fasciola hepatica infection and diethylnitrosamine (DENA) intoxication. The contents of zinc (Zn), iron (Fe), copper (Cu), cobalt (Co), molybdenum (Mo), chrome (Cr), selenium (Se), rubidium (Rb) and bromine (Br) were in...

Thermostable biologically active substances (BAS), inhibitors of cell proliferation, are isolated from livers of healthy and Trichinella spiralis infected rats. Their effects are investigated on cell viability of primary Graffi myeloid tumor cell cultures. The inhibiting effect of BAS isolated from livers of T. spiralis infected rats on tumor cell...

The impact of experimental infection with Ttichinella spiralis on oxidative / antioxidant status in mice was traced. The activity of the enzyme superoxide dismutase (SOD), glutathione peroxidase (GPx), the level of vitamins A, C and E, and the content of malondialdehyde (MDA) were studied. It was found that MDA, SOD and GPx in infected mice have be...

The contents of vitamins A, C, E and B12 in liver of lambs uninfected and infected with gastrointestinal nematode Haemonchus contortus was determined. The results showed a decrease in the values of the four vitamins of the group of infected lambs compared to the control group. Lower levels of these vitamins has been associated with the development...

Оглавление книги: Современные тенденции развития биогеохимии (ред. В.В. Ермаков), 2016. https://www.researchgate.net/publication/315718354;

Abstract: the Author's team. Part of the text in English. ISBN 978-5-905049-15-6. Ed. by V. V. Ermakov. Among the co - authors of the book are members of RAS, employees of several institutes of RAS, employees of MSU. Lomonosov's. Part of the book includes articles syso A. I., Alekseenko V. A., E. A. Romankevich etc..... THE QUESTION OF THE MANIFEST...

Contents of some liver trace elements, antioxidants and quantitative parameters of the state of body health were investigated in chicks after experimental Ascaridia galli infection and basic zinc-copper (Zn-Cu) salt application. The levels of trace elements Zn and Cu and antioxidants vitamin C and vitamin E were decreased in the livers of A. galli...

The study was carried out on the biochemical indices (oxidant/ antioxidant status) and histological response after implantation of modified hydroxyapatite in rat calvarial defects. Three groups of rats were used in the experiment: 1st group – control – healthy animals, 2nd group – rats received critical size skull defect (CSD) with no scaffold impl...

The study compares in vivo the behavior of Zn-beta TCP in a past form implanted in bone of rabbits. Biochemical hematological parameters were studied. Their levels were in the physiological limits. No sign effects were marked in all animals after operation with autologous and Zn-beta TCP implants. No significant changes in the markers of bone metab...

Copper, zinc, iron, manganese and selenium are essential trace elements playing roles in various metabolic processes. The aim of the present study was to assess the concentration of Cu, Zn, Fe, Mn and Se in the whole blood of Ovis aries experimentally infected with Haemonchus contortus (Nematoda). Lower blood levels of Cu, Zn, Fe, Co and Se in the...

Glycosyltransferases are present in the Golgi apparatus in a membrane- bound form and are released from cells after cleavage by certain proteases. N-acetylglucosaminyltransferase catalyzes branching of N-acetylglucosamine on asparagine-linked oligosaccharides of the cell proteins. Their expressions are elevated during malignant transformation in va...

The present study was carried out on the biochemical indices (oxidant/ antioxidant status) and histological response after implantation of modified cements based on DCPA and TTCP as solid phase and tartaric or ascorbic acids as liquid phase in rat calvarial defects. Three groups of rats were used in the experiment: 1st gr.- control (received a crit...

The aim of this study was to investigate the levels of antioxidant trace elements (zinc, copper, selenium) and the activity of related metalloenzymes (superoxide dismutase: SOD and glutathione peroxidase, GPx) in the blood of rabbits experimentally infected with Eimeria stiedae. The results showed that blood Se and serum Zn concentrations, the GPx...

This study is a review of investigating on biochemical markers of bone metabolism in various experimental animals. The biochemical markers are investigated in animal serum, soft tissues around bone defects and bone implants. Alkaline phosphatise is investigated the most frequently as a biochemical marker of bone metabolism in experimental animals.

Thermolable biologically active substances (BASes) were isolated from healthy and Fasciola hepatica infected rat spleens. The effect of the newly isolated BASes was studied in vitro on nonactivated lymphocyte cell cultures. An inhibiting immunomodulating effect of the BAS isolated from F. hepatica infected rat spleens was demonstrated.

The study was carried out on the biochemical indices (oxidant/ antioxidant status) and histological response after implantation of modified hydroxyapatite in rat calvarial defects. Three groups of rats were used in the experiment: 1st group – control – healthy animals, 2nd group – rats received critical size skull defect (CSD) with no scaffold impl...

Gastrointestinal nematodoses are among the most destructive pathologies affecting ovine production, given high prevalence in flocks and serious consequences of infection. The gastrointestinal nematodes from the Haemonchns species are major blood feeding parasite of ruminants worldwide. Infection can lead to anaemia, emaciation, oedema and gastric d...

In the system investigated “Cyprinus carpio—invaded with Khawia sinensis and uninvaded” - the content of Cr, Cu and Se is higher in the parasite in comparison with that of C. carpio. In the host (invaded and uninvaded) the Fe level is similar to that of K. sinensis. The content of Zn and Co is lower in K. sinensis in comparison with that of C. carp...

Sialic acids a large family of neuraminic acid derivatives, are acidic monosaccharides, present as terminal component of oligosaccharide chains of many glycoproteins and glycolipids. They play an important role in many physiological and pathological processes like cell-cell communications, cell-substrate interaction, adhesion, maintenance of serum...

Eimeriosis is one of the most common parasitic diseases in domestic rabbit (Oryctolagus cuniculus) causing high mortality. In vitro culturing in cells culture of Eimeria allows more accurate studies in the absence of a complex immune response of the host to decode a complex host - parasite interactions. The present study describes a methodology for...

The aim of this study was to investigate the levels of antioxidant trace elements (zinc, copper, selenium) and the activity of related metalloenzymes (superoxide dismutase: SOD and glutathione peroxidase, GPx) in the blood of rabbits experimentally infected with Eimeria stiedae. The results showed that blood Se and serum Zn concentrations, the GPx...

Two types of calcium phosphate materials prospective as bone implants were prepared in the shape of granules and their biochemical behavior was tested by in vivo studies: (i) composite materials consisting of gelatin and bi-phase ion modified calcium phosphate Mg,Zn-(HA + β-TCP); and (ii) ceramics of ion modified calcium phosphate Mg,Zn-(HA + β-TCP...

It is known that helminthological status of animals depends on the content of heavy metals in the environment and feed [12]. But in some cases, salts of heavy metals (copper) are effective therapeutic agents at some parasitic infestations. This applies to birds helminthoses. Avian eimeriosis is one of the most common diseases in countries with indu...

Eimeria tenella (простейшие). Состояние организма, степень инвазии паразитом, содержание в печени витамина Е, меди и концентрации в плазме малондиальдегида (МДА) бьши оценени. Результатм показали улучшение веса тела, снижение конверсии корма, повишение в печени витамина Е и меди, а также повьшенное содержание МДА. Данни е зтого зксперимента показьш...

Biochemical blood indices (Ca, P, total and bone alkaline phosphatase and osteocalcin) in rats with implanted in tibia hydroxyapatite-gelatine-xanthan gum composite materials were studied. Two types of composites with high temperature treated ceramic powder and additionally high energy treated ceramic powder were applied. The result showed reductio...

The effect o f an organic compound 2 Gly.MnCl2.2H2O on broilers challenged with Eimeria tenella was investigated. Antioxidant imbalance and reduced body weight was found in the infect? ed broilers. They were with improved weight and the oocyst index was reduced after Mn supple? mentation. Diet supplementation with 2 Gly.MnCl2.2H2O could be benefici...

The effect of tribasic copper chloride (TBCC) on broiler chickens experimentally infected with Eimeria tenella (Protozoa) was investigated. Body performance, parasite burden, liver and plasma vitamin E and copper level were measured. The results showed improved body weight gain, reduced feed conversion ratio, elevated liver vitamin E and Cu content...

The large stomach worm, Haemonchus contortus, commonly known as “the barber’s pole worm”, is a blood-sucking nematode found in the abomasa of sheep and goats. Haemonchosis is a serious health problem which causes lower production due to high morbidity, mortality, and cost of treatment and control measures. A range of hematological measures were sig...

Calcium phosphate ceramics, cements and pastes are suitable bone materials in orthopedic, reconstructive and maxillofacial surgery. Composite scaffolds on the base of Zn-modified-tricalcium phosphate and mixtures of xanthan gum, caragenan, saccharose and gelatin were studied as possible implants. The in vivo behaviour of them was tested in experime...

The objective of this study was to determine the efficacy of bone scaffolds of composite calcium phosphate/poly L-Lactide acid (PLLA) in vivo (in a rat model). Nano-hydroxyapatite/poly l-lactide acid (HAP/PLLA) composite scaffolds were synthesized and applied in a calvarial bone defects. They were created in rat calvarias. The reactions of bone hea...

Parasitic diseases seem to be a causative source of oxidative stress, as, several studies have reported on the presence of oxidative stress in humans and animals infected with parasites as well as antioxidant defense systems that exist between parasites and their hosts. Ruminant gastrointestinal nematode infections are common and widespread, howeve...

Human activities have altered natural concentrations of many substances in the environment and added numerous new chemicals. In general there is a relationship between metal concentrations among several species. Bioaccumulation capacity has been well documented for some groups of organisms, as well as for some metals. Parasites are ubiquitous. Ther...

The effect of experimental infection with Haemonchus contortus on trace element levels was studied in lambs. The levels of Cu, Fe, Zn, and Se was determined in serum, liver and muscle of lambs infected with Haemonchus contortus and uninfected ones. The serum trace element levels were changed but they were not significantly changed in liver and musc...

The development of orthopedic and dental implants has taken place for many years. For establishment whether a new biomaterial conforms to the requirements of biocompatibility and stability prior to clinical application, it must be test initially in vitro and in vivo. Animal models are essential for evaluating biocompatibility, tissue response and m...

In our in vitro studies we have been testing (including comparatively) Earthworms (as a model) and nematodes (ascaris of pigs and poultry), which were clinically extracted, and have been setting them on Engelman's shift in temperature-controlled physiological fluid and recording their spontaneous or toxic induced movements (from convulsions to deat...

The purpose of our study is to examine the lung oxidative stress status in mouflons naturally infected with the nematode Diciyocaulus viviparus by determination of the malondyaldehyde (MDA) concentration as a biomarker of lipid peroxidation and its relation to the antioxidant status (glutathione, vitamin A, vitamin E, vitamin C, enzymes SOD, CAT, G...

The oxidative-antioxidant and zinc status of mice infected with Trichinella spiralis and supplemented/unsupplemented with 2Gly.ZnCl2.2H2O was studied. The markers were malondialdehyde content, glutathione peroxidase and Cu,Zn- superoxide dismutase activity. Zinc levels were determined in liver and plasma. The parasite burden in mice was studied. Tr...

Our study was to assess the accumulation of heavy metals in hares (Lepus europeaus Pallas, 1778) infected with endoparasites. Ihe effect of cesfodes Mosgovayia pectinata or nematodes Tichostrcngylus retortaefonnis on Cd, Pb, Co, Hg, Zn and Cu concentrafions uvas esfab/rshed. Hosfs infected with cestodes had lower Cd and Pb levels than hosts infecte...

The effect of sodium selenite on the antioxidant status in broiler chickens infected with Eimeria acervulina was studied. Antioxidant status was measured via blood plasma malonyl dialdehyde (MDA), serumglutathione peroxidase (GPx), erythrocyte catalase (CAT) and superoxide dismutase (SOD) activities and plasma levels of selenium (Se), vitamins A, C...

The study compares the in vivo behavior of three calcium phosphates (mono-phase Zn-f$ Mcaicium phosphate. Mg—$ tricalcium phosphate and a bi-phase mixture of hydroxyapatite and B-tricaidum phosphate) in a paste form and implanted in rabbits. Radiological, histological, and biochemical studies were done. The phase composition and ion {Zn and Mg) mod...

The structural, functional and metabolic changes in host tissues caused during penetration, migration and localization of helminths in the host body, are associated with specific changes in trace element levels in body tissues, in- cluding these of liver and spleen. The aim of the present study is determination of the quantities of trace elements z...

Parasitic diseases seem to be a causative source of oxidative stress, indeed, several studies have reported on the presence of oxidative stress in humans and animals infected with parasites as well as antioxidant defense mechanism that exist between parasites and their hosts. Ruminant gastrointestinal nematode infections are common and widespread,...