Margarita Chirkina

N.M. Emanuel Institute of Biochemical Physics RAS · quantitative oncology laboratory

The applicability of beam-plasma chemical reactors generating cold hybrid plasma for the production of noncytotoxic polymeric surfaces with high hydrophilicity and good biocompatibility with human fibroblast culture and human red blood cells was studied. Oxygen hybrid plasma was excited by the joint action of a continuous scanning electron beam and...

The combination of two or more medications is increasingly more common in the development of new treatment guidelines for common diseases. Thus, the use of combinations of histone deacetylase inhibitors with chemotherapeutic agents is a current trend in solid tumor treatment. The aim of this study was to investigate an effective vorinostat (SAHA) t...

Metal–organic Frameworks (MOFs) are widely used in the biomedical industry. In this study, we developed a new method for obtaining a metal-organic structure of strontium and terephthalic acid, Sr(BDC), and...

Biocompatible, biodegradable polymers are actively used as drug delivery systems and as tissue engineering scaffolds. The hydrothermal aging of polymers, including degradation of the microstructure and changes of mechanical properties directly depends on environmental conditions. In vitro studies in a model media cannot contain and describe all the...

The effect of the hemin (Hmi) on the structure and properties of nanocomposite electrospun materials based on poly-3-hydroxybutyrate (PHB) is discussed in the article. The additive significantly affected the morphology of fibers allowed to produce more elastic material and provided high antimicrobial activity. The article considers also the impact...

Previous studies have demonstrated the applicability of alpha-fetoprotein or its receptor-binding domain fused to the 6-histidine tag at the C-terminus (rAFP3D-His6) as vectors for targeted delivery of antitumor agents. The His6 tag is undesirable for further preclinical trials. Therefore, we designed a recombinant protein rAFP3D without any affine...

While histone deacetylase inhibitors, such as vorinostat, demonstrate significant effect against hematological cancers, its application for solid tumors treatment is limited. However, there is strong evidence that combinatorial administration of vorinostat and genotoxic agents (e.g., doxorubicin) enhance the antitumoral action of both drugs against...

Currently, molecular dynamics simulation is being widely applied to predict drug-polymer interaction, and to optimize drug delivery systems. Our study describes a combination of in silico and in vitro approaches aimed at improvement in polymer-based nanoparticle design for cancer treatment. We applied the PASS service to predict the biological acti...

Background: Serious side effects caused by paclitaxel formulation, containing toxic solubilizer Cremophor ® EL, and its nonspecific accumulation greatly limit clinical paclitaxel application. Aim: To design paclitaxel-loaded copolymer of lactic and glycolic acids nanoparticles decorated with alpha-fetoprotein third domain (rAFP3d-NP) to increase pa...

The conventional targeted delivery of chemotherapeutic and diagnostic agents utilizing nanocarriers is a promising approach for cancer theranostics. Unfortunately, this approach often faces hindered tumor access that decreases the therapeutic index and limits the further clinical translation of a developing drug. Here, we demonstrated a strategy of...

Pheophorbide a 17-diethylene glycol ester (XL-8), is a promising high-active derivative of known photosensitizer chlorin e6 used in photodynamic therapy. However, high lipophilicity and poor tumor accumulation limit XL-8 therapeutic application. We developed a novel XL-8 loaded with poly(D,L-lactide-co-glycolide) nanoparticles using the single emul...

Background Difficult to express peptides are usually produced by co-expression with fusion partners. In this case, a significant mass part of the recombinant product falls on the subsequently removed fusion partner. On the other hand, multimerization of peptides is known to improve its proteolytic stability in E. coli due to the inclusion of body f...

Introduction. The use of the anticancer drug paclitaxel is limited due to its high toxicity and lipophilicity. A new polymer composition of paclitaxel has been proposed, which provides targeted transport of the drug into tumor cells and improves its safety. Aim. Method development for preparation of a novel paclitaxel formulation consisting of a co...

The selection of technological parameters for nanoparticle formulation represents a complicated development phase. Therefore, the statistical analysis based on Box–Behnken methodology is widely used to optimize technological processes, including poly(lactic-co-glycolic acid) nanoparticle formulation. In this study, we applied a two-level three-fact...

Previous studies have demonstrated the applicability of alpha-fetoprotein or its receptorbinding domain fused to the 6-histidine tag at the C-terminus (rAFP3D-His6) as vectors for targeted delivery of antitumor agents. This tag is undesirable for further preclinical trials. Therefore, we designed a recombinant protein rAFP3D without any affine tags...

Relevance. A chemotherapeutic agentpaclitaxel (Ptx) has limited usage because of the pronounced side effects of excipients and the non-specificity of Ptx action. We proposed a novel Ptx formulation for targeted therapy of malignant neoplasms and reducing the level of side effects. Aim of the study was to develop a normative documentation for standa...

Objectives: The goal of this study was to develop sample preparation method and validate the HPLC method for precise determination of paclitaxel (Ptx) in PLGA submicron particles conjugated with protein vector molecule. Methods: Ptx loaded PLGA submicron particles were formulated by a single emulsification method. PLGA submicron particles were conj...

The paper describes the expression, purification and isolation of native sequence human alpha-fetoprotein recombinant receptor-binding domain and evaluation its functional activity for specific targeting to tumor cells.

A UV spectrophotometric method for quantitative determination of paclitaxel in a conjugate of poly(lac-tic-glycolic acid) (PLGA) copolymer nanoparticles and a protein vector (recombinant third domain of alpha -fetoprotein) was developed. Paclitaxel was determined at wavelength 260 ± 2 nm. The proposed method showed specificity, linearity (R 2 = 0.9...

The history of metalloporphyrins dates back more than 200 years ago. Metalloporphyrins are excellent catalysts, capable of forming supramolecular systems, participate in oxygen photosynthesis, transport, and used as contrast agents or superoxide dismutase mimetics. Today, metalloporphyrins represent complexes of conjugated π-electron system and met...

Разработан метод количественного определения паклитаксела в составе конъюгата наночастиц на основе сополимера молочной и гликолевой кислот и белковой векторной молекулы (рекомбинантным третьим доменом альфа-фетопротеина) с помощью УФ-спектрометрии. Определение паклитаксела проводили при длине волны (260 ± 2) нм. Доказаны специфичность, линейность (...

FIELD: medicine. SUBSTANCE: invention relates to an anti-tumour agent which is a ready dosage form in the form of a lyophilizate for parenteral administration for targeted delivery of particlesinto the affected organs and tissues, as well as for prolonged action. Anti-tumour agent contains an alpha-hydroxybenzole propane acid derivative, a lactic a...

New high-molecular-weight contrast agents based on polyamidoamine (PAMAM) dendrimers for targeted imaging of malignant tumors characterized by overexpression of human epidermal growth factor receptor (EGFR) and human alpha-fetoprotein receptor (RECAF) were designed. Conjugates of second (G2) and third (G3) generation polyamidoamine dendrimers with...

Разработаны новые высокомолекулярные контрастные агенты на основе полиамидоаминовых (PAMAM) дендримеров для селективной визуализации злокачественных новообразований, характеризующихся гиперэкспрессией рецептора эпидермального фактора роста (REGF) и рецептора альфа-фетопротеина (RECAF). Получены конъюгаты полиамидоаминовых дендримеров второго (G2) и...

Reactive oxygen species are generated by the redox reaction involving metalloporphyrin and ascorbic acid (AA) and lead to oxidative stress followed by cancer cell death. Polymer particles based on the copolymer of lactic and glycolic acid (PLGA) containing FeIIICl-tetraphenylporphyrin (FeClTPP) were prepared and characterized. These particles in co...

В результате окислительно-восстановительной реакции с участием металлопорфирина и аскорбиновой кислоты (АК) образуются активные формы кислорода, приводящие к окислительному стрессу в опухолевых клетках и последующей их гибели. Получены и охарактеризованы полимерные частицы на основе сополимера молочной и гликолевой кислот (ПМГК), содержащие FeIIICl...

The mechanisms of binary catalyst therapy (BCT) and photodynamic therapy (PDT) are based on the formation of reactive oxygen species (ROS). This ROS formation results from specific chemical reactions. In BCT, light exposure does not necessarily initiate ROS formation and BCT application is not limited to regions of tissues that are accessible to il...

Silybin (Slb) and ursodeoxycholic acid (UDCA) are hepatoprotectors used in the pathogenetic therapy of liver and biliary tract. However, low bioavailability of these drugs restricts their application. In order to solve this problem, Slb and UDCA were incorporated into polymer carriers based on polylactic acid and poly(lactic-co-glycolic acid) by na...

To the article “Antitumor activity of carboplatin in the composition of a copolymer of lactic and glycolic acids,” by E. D. Nikolskaya, O. A. Zhunina, N. G. Yabbarov, V. A. Zenin, O. G. Tereshchenko, M. V. Fomicheva, M. B. Sokol, A. V. Lobanov, and E. S. Severin, Vol. 66, No. 10, pp. 1867–1872, October, 2017. In the original international version o...

Разработана полимерная форма джозамицина путем включения данного антибиотика в частицы сферической формы субмикронного размера из сополимера молочной и гликолевой кислот (ПМГК 50/50). Средний диаметр частиц не превышает 150 нм, а значение дзета-потенциала составляет -35 мВ. Полимерная форма обладает антибактериальной активностью в отношении грампол...

Polymer particles containing carboplatin (CPt) were developed by the inclusion of this antitumor agent in a copolymer of lactic and glycolic acids (PLGA-COOH 50/50). The polymer particles were found to have a spherical form with an average diameter not exceeding 200 nm, the ζ-potential is equal to–32.2±1 mV. The CPt-loaded polymer particles possess...