Margaret Frank

Margaret Frank
Donald Danforth Plant Science Center · Chitwood Lab

Doctor of Philosophy

About

23
Publications
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558
Citations

Publications

Publications (23)
Article
Color patterning contributes to important plant traits that influence ecological interactions, horticultural breeding, and agricultural performance. High-throughput phenotyping of color is valuable for understanding plant biology and selecting for traits related to color during plant breeding. Here we present ColourQuant, an automated high-throughp...
Article
Full-text available
Coleus (Coleus scutellarioides) is a popular ornamental plant that exhibits a diverse array of foliar color patterns. New cultivars are currently hand selected by both amateur and experienced plant breeders. In this study, we reimagine breeding for color patterning using a quantitative color analysis framework. Despite impressive advances in high-t...
Article
Full-text available
Premise: As a leaf expands, its shape dynamically changes. Previously, we documented an allometric relationship between vein and blade area in grapevine leaves. Larger leaves have a smaller ratio of primary and secondary vein area relative to blade area compared to smaller leaves. We sought to use allometry as an indicator of leaf size and plastic...
Preprint
Coleus is a popular ornamental plant that exhibits a diverse array of foliar color patterns. New cultivars are currently hand selected by both amateur and experienced plant breeders. In this study, we reimagine coleus breeding using a quantitative color analysis framework. Despite impressive advances in high-throughput data collection and processin...
Article
Full-text available
Premise: Leaf morphology is dynamic, continuously deforming during leaf expansion and among leaves within a shoot. Here, we measured the leaf morphology of more than 200 grapevines (Vitis spp.) over four years and modeled changes in leaf shape along the shoot to determine whether a composite leaf shape comprising all the leaves from a single shoot...
Preprint
Full-text available
Premise of study: Leaf morphology is dynamic, continuously deforming during leaf expansion and among leaves within a shoot. We measured leaf morphology from over 200 vines over four years, and modeled changes in leaf shape along the shoot to determine if a composite shape of shapes can better capture variation and predict species identity compared...
Preprint
Full-text available
Colour patterning contributes to important plant traits that influence ecological interactions, horticultural breeding, and agricultural performance. High-throughput phenotyping of colour is valuable for understanding plant biology and selecting for traits related to colour during plant breeding. Here we present ColourQuant, an automated high-throu...
Article
Full-text available
Current morphometric methods that comprehensively measure shape cannot compare the disparate leaf shapes found in seed plants and are sensitive to processing artifacts. We explore the use of persistent homology, a topological method applied as a filtration across simplicial complexes (or more simply, a method to measure topological features of spac...
Article
Full-text available
Thicker leaves allow plants to grow in water-limited conditions. However, our understanding of the genetic underpinnings of this highly functional leaf shape trait is poor. We used a custom-built confocal profilometer to directly measure leaf thickness in a set of introgression lines (ILs) derived from the desert tomato species Solanum pennellii, a...
Preprint
Full-text available
Thicker leaves allow plants to grow in water-limited conditions. However, our understanding of the genetic underpinnings of this highly functional leaf shape trait is poor. We used a custom-built confocal profilometer to directly measure leaf thickness in a set of introgression lines (ILs) derived from the desert tomato species Solanum pennellii ,...
Preprint
Full-text available
Current morphometric methods that comprehensively measure shape cannot compare the disparate leaf shapes found in seed plants and are sensitive to processing artifacts. We explore the use of persistent homology, a topological method applied across the scales of a function, to overcome these limitations. The described method isolates subsets of shap...
Preprint
Full-text available
Genetic contributions to plant morphology are not partitioned between shoots and roots. Yet, shoot and root architectures are rarely measured in the same plants. Even if shoot and root architectures are both studied, the application of mathematical methods flexible enough to accommodate the disparate topologies and shapes within a plant, and across...
Article
Full-text available
Leaf shape varies spectacularly among plants. Leaves are the primary source of photoassimilate in crop plants, and understanding the genetic basis of variation in leaf morphology is critical to improving agricultural productivity. Leaf shape played a unique role in cotton improvement, as breeders have selected for entire and lobed leaf morphs resul...
Preprint
Full-text available
Leaf shape is spectacularly diverse. As the primary source of photo-assimilate in major crops, understanding the evolutionary and environmentally induced changes in leaf morphology are critical to improving agricultural productivity. The role of leaf shape in cotton domestication is unique, as breeders have purposefully selected for entire and lobe...
Article
Chimeras – organisms that are composed of cells of more than one genotype – captured the human imagination long before they were formally described and used in the laboratory. These organisms owe their namesake to a fire-breathing monster from Greek mythology that has the head of a lion, the body of a goat, and the tail of a serpent. The first desc...
Preprint
Chimeras – organisms that are composed of cells of more than one genotype – captured the human imagination long before they were formally described and used in the laboratory. These organisms owe their namesake to a fire-breathing monster from Greek mythology that has the head of a lion, the body of a goat, and the tail of a serpent. The first desc...
Article
Full-text available
Grafting is an ancient agricultural practice that joins the root system (rootstock) of one plant to the shoot (scion) of another. It is most commonly employed in woody perennial crops to indirectly manipulate scion phenotype. While recent research has focused on scions, here we investigate rootstocks, the lesser-known half of the perennial crop equ...
Article
Haploid moss gametophytes harbor distinct stem cells types, which include tip cells that divide in single planes to generate filamentous protonemata and bud cells that divide in three planes to yield axial gametophore shoots. This transition from filamentous to triplanar growth occurs progressively, during the moss life cycle, and is thought to mir...
Article
Shoot apical meristem (SAM) structure varies markedly within the land plants. The SAMs of many seedless vascular plants contain a conspicuous inverted, pyramidal cell called the apical cell (AC), which is unidentified in angiosperms. In this study, we use transcriptomic sequencing with precise laser microdissections of meristem subdomains to define...
Article
Full-text available
Alternation of generations, in which the haploid and diploid stages of the life cycle are each represented by multicellular forms that differ in their morphology, is a defining feature of the land plants (embryophytes). Anciently derived lineages of embryophytes grow predominately in the haploid gametophytic generation from apical cells that give r...

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Projects

Projects (3)
Archived project
Coleus leaves are the butterfly wings of the plant world. This decorative plant exhibits tremendous color patterning diversity. In 2015, we scanned >34,000 different Coleus leaves, with the goal of quantitatively describing color patterning, connecting color features with the commercial value of Coleus varieties, and approximating the heritability of patterns. Click here to participate in our citizen science survey "Coleus Colors": goo.gl/w5Prqn
Archived project
We are linking the anatomical reconnection of xylem and phloem cells during graft junction formation with the functional restoration of water and sugar transport (measured by Positron Emission Tomography). The goals of this project are three-fold: (1) to establish a precise timeline for vascular reformation during graft junction formation, (2) understand how anatomical features are linked with function, (3) explore PET imaging technology for plant research. This project has an associated outreach video that was made by ScienceIRL: https://www.youtube.com/watch?v=ANjIuTnQxEc
Archived project
I'm investigating transcriptomic, ionomic, and morphological changes that are triggered by grafting genotypically and architecturally distinct root and shoot systems together.