Marek Pienkowski

Allergic Diseases Asthma and Immunology Clinic

W terminologii kosmetologicznej i medycznej stosowane są dwie definicje terminu „emolient”. Pierwsza dotyczy substancji hydrofobowej dodawanej do kosmetyku w celu natłuszczenia, zmiękczenia, wygładzenia i pośredniego nawilżenia skóry. Druga definiuje emolient jako gotowy preparat nawilżający i natłuszczający skórę. Badanie rynku zostało przeprowadz...

The advent of transgenic technology has provided methods for the production of pharmaceuticals by the isolation of these proteins from transgenic animals. The mammary gland has been focused on as a bioreactor, since milk is easily collected from lactating animals and protein production can be expressed at very high levels, including hormones and en...

A novel technique of chimeric somatic cell cloning was applied to produce a transgenic rabbit (NT20). Karyoplasts of transgenic adult skin fibroblasts with Tg(Wap-GH1) gene construct as a marker were microsurgically transferred into one, previously enucleated, blastomere of 2-cell non-transgenic embryos, while the second one remained intact. The re...

In this paper, the effect of osteoprotegerin (OPG) on slowing down the resorption process of heterotopically induced bone tissue is described. The induced ossicle is resorbed ex inactivitate. This system mimics osteoporosis in immobilised skeletal bones. Bone induction was achieved in BALB/c mice after the injection of the suspension of 3 x 10(6) H...

In this study we compared the effects of preculture storage of ovaries, IVM medium, a reduced O(2) atmosphere and duration of culture on in vitro maturation (IVM) of domestic cat oocytes. One randomly selected ovary of each pair (69 pairs) was stored in PBS at 10 degrees C for 16-24h before oocyte recovery. The second ovary from each pair was used...

The gene construct WAP(6xHisThr):hGH containing the entire human growth hormone gene (hGH) under the rat whey acidic protein (WAP) promoter regulating the expression in mammary glands of mammals was prepared. The 5' end of the gene was modified by the addition of a sequence encoding six histidine residues and a sequence recognized by thrombin. The...

This work was undertaken in order to study the developmental competence of nuclear transfer feline embryos with regard to the recipient-cytoplast's age and type of somatic cells used as donor nuclei. Oocytes were recovered by mincing the ovaries in HEPES-buffered TCM-199. Selected cumulus-oocyte complexes (COCs) with compact cumulus cell mass and a...

A penicillin-allergic patient who required therapy with beta-lactam antibiotics was desensitized with increasing parenteral doses of benzylpenicillin. After desensitization, the patient tolerated, without signs of allergic reaction, intravenous piperacillin. Immunologic studies were undertaken to investigate humoral and cellular changes accompanyin...

With a skin blister technique in which the mediators generated by the trauma of forming the blister are allowed to subside, we have collected human interstitial skin fluid during the course of allergic reactions to ragweed, and measured levels of histamine and prostaglandin D2 (PGD2). Of 18 ragweed-allergic individuals tested, 11 developed both an...

We have shown that fluids collected from antigen-challenged skin blisters during the late phase reaction cause the release of substantial amounts of histamine (means = 42%, n = 14) from human basophils in vitro. Control fluids collected either during the immediate phase or from an unchallenged blister released less than or equal to 10% histamine fr...

The cutaneous late-phase reaction (LPR) to ragweed was studied in untreated ragweed-allergic individuals and patients receiving 3 to 5 years of immunotherapy demonstrating clinical improvement. The magnitude of immediate skin reactions and the initial levels of the specific IgE and IgG antiragweed antibodies were similar in both groups. The LPR was...

The treatment of mouse blastocysts with cyclic AMP (cAMP) (1 mM) in vitro resulted in a 5-fold decrease in the percentage of embryos developing germ layers as compared with controls. However, outgrowth of trophoblastic cells from embryos was unaffected by cAMP. Theophylline (1 mM) was found to be synergistic to the inhibitory effect of cAMP on the...

Congenitally athymic homozygous (nu/nu) mice were shown to be significantly more susceptible to Trypanosoma cruzi infection than their thymus-bearing heterozygous (nu/+) littermates, as measured by increased parasitemia, mortality rate, and shortened survival time. In addition, transplantation of neonatal thymus into athymic mice reestablished norm...

Vaginal adenosis biopsy specimens from 10 patients exposed in utero to diethylstilbestrol were transplanted for 30 days into athymic (nude) mice. Almost all grafts were recovered, and they had morphologic features closely resembling those of the original biopsy specimens, i.e., cystic, complex, and simple occult glands covered mainly with an endoce...

A visual display board was designed to aid in the management of a barrier sustained athymic (nude) mouse colony. The board displayed pertinent information for breeding and weaning, including phenotype and age for each animal in the colony. In addition, the board showed the availability and current status of experimental groups. This system provided...

Each of 10 biopsy specimens of benign human breast tumors, obtained from 10 patients, was cut into slices (30 slices/specimen) and transplanted s.c. into 3 female athymic nude mice (30 mice, 10 grafts/mouse). Of the 3 mice in each of the 10 triads, 2 mice were intact and 1 mouse was ovariectomized 30 days before transplantation. Ten days after tran...

A microculture assay utilizing [3H]uridine incorporation was developed to test murine spleen lymphocyte immunoreactivity in vitro. Parameters of the culture technique which included cell density, doses of LPS, Con A, PHA, [3H]uridine levels, and length of culture time were investigated. Responses were detectable at 4 h for all 3 mitogens, with labe...

Mouse blastocysts were grown in vitro and the ultrastructure of resulting egg cylinders was compared with the ultrastructure of egg cylinders isolated from uterus. Egg cylinders grown in vitro had two or three germ layers and were attached to the bottom of the dish through the layer of trophoblastic cells. The entoderm was composed of two cell type...

A virus originally isolated from cell cultures obtained by lysolecithin-induced fusion of human multiple sclerosis brain cells with CV-1 cells has been analyzed for its antigenic, RNA, and polypeptide compositions, and for selective biological properties. Our findings establish that this isolate, designated 6/94 virus, contains a 50S RNA genome and...

Mouse blastocysts grown in a simple, easily controlled culture for 5 days developed into egg cylinder-like structures. The removal of zona pellucida and treatment with proteolytic enzymes promoted the development of blastocysts in vitro. Egg cylinders similarly cultured grew and showed signs of early organogenesis, i.e., beating heart, blood island...

Mouse eggs were parthenogenetically stimulated with hyaluronidase and osmotic shock and the resulting two-, four- and eight-cell stage embryos examined with the electron microscope. The ultrastructure of parthenogenones during cleavage showed numerous differences when compared with normal development. Cortical reaction was absent and there were no...

Unfertilized eggs and preimplantation mouse embryos had the same binding capacity for Concanavalin A. Concanavalin A in doses of 10 μg/ml agglutinated preimplantation embryos and the embryos derived from parthenogenetically stimulated eggs, but did not agglutinate unfertilized eggs. Unfertilized eggs became agglutinable with Concanavalin A in doses...

Unfertilized eggs and preimplantation mouse embryos had the same binding capacity for Concanavalin A. Concanavalin A in doses of 10 μg/ml agglutinated preimplantation embryos and the embryos derived from parthenogenetically stimulated eggs, but did not agglutinate unfertilized eggs. Unfertilized eggs became agglutinable with Concanavalin A in doses...

Two celled mouse embryos, blastocysts, and egg cylinders were exposed to simian virus 40 (SV40) and polyoma virus. Uptake of SV40 but not of polyoma was found when 2 celled embryos were exposed to virus. Blastocysts and egg cylinders exposed to SV40 or polyoma showed adsorption and uptake of virus particles. When infected blastocysts were grown in...

Electron microscope observations of 2 celled embryos from 8 strains of laboratory mice revealed an abundance of intracisternally located type A particles. A much smaller number of the same type particle was seen in blastocysts from the same strains. Blastocysts and egg cylinders from highly leukemogenic AKR mice also contained budding and extracell...

The effects of Concanavalin A (Con A) on the percentage of blast cells and 3H thymidine incorporating cells, as well as on DNA content and dry mass were investigated and compared with those of phytohaemagglutinin (PHA). In the presence of Con A, the percentage of blast cells in 72 hr cultures is much smaller than in those with PHA, whereas the perc...

The quantitative description of the morphology of chromatin of Feulgen-stained lymphocytes were done by means of the Image Analysing Computer Quantimet. The description consisted of the measurements of the areas of chromatin with different optical density. Three types of cells were analysed: resting lymphocytes, lymphocytes stimulated in vitro with...

Es wird gefunden, dass Penicillin zur RNS-Synthese whrend der ersten 4 h der Kultur stimuliert und 7–13% der Blutlymphozyten bei Menschen eine Sensibilisierung gegen dieses Antibioticum aufweisen. Der ermittelte Prozentsatz gleicht dem der unter Penicillineinfluss transformierenden Lymphozyten bei ein und derselben Person. Ein Einfluss von Penicill...

PHYTOHAEMAGGLUTININ is known to stimulate the human peripheral blood lymphocytes to enlarge and divide1. The process of the morphological transformation2 of these cells through nucleolated prolymphocytes into blastoid cells is accompanied by cytochemical3 changes and the acquisition of the ability to synthesize RNA and DNA4-6.