Marco Vergani

Flex | FLEXTRONICS

Down scaling of microfluidic cell culture and detection devices for electrochemical monitoring is mostly focused on the miniaturization of the microfluidic chips which are often designed for specific applications and therefore they lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with...

In this paper we demonstrate the fabrication and electrochemical characterization of a microchip with 12 identical but individually addressable electrochemical measuring sites, each consisting of a set of interdigitated electrodes acting as a working electrode as well as two circular electrodes functioning as a counter and reference electrode in cl...

A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. These widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10 μm interdigitated microelectrodes using a versatile custom-made monitoring platf...

A surface modification of interdigitated gold microelectrodes (IDEs) with a doped polypyrrole (PPy) film for detection of dopamine released from populations of differentiated PC12 cells is presented. A thin PPy layer was potentiostatically electropolymerized from an aqueous pyrrole solution onto electrode surfaces. The conducting polymer film was d...

Since the use of impedance measurements for label-free monitoring of cells has become widespread but still the choice of sensing configuration is not unique though crucial for a quantitative interpretation of data, we demonstrate the application of a novel custom multipotentiostat platform to study optimal detection strategies. Electrochemical Impe...

A novel potentiostat containing 54 current amplifiers matched to an array of custom-fabricated 5μm microelectrodes for electrochemical imaging of released neurotransmitters is presented. The board is integrated with a programmable microfluidic cell culture system and the whole assembly is thin and compact enough to be placed under the objective of...

Arrays of microelectrodes for electrochemical biosensing are commonly fabricated on standard silicon wafers. This choice positively profits from the optimized microelectronic technology, offering micrometric spatial resolution and the possibility to integrate electronic processing capabilities directly on the sensing chips. This paper analyzes the...

An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data a...

The increase in the understanding of the physical and functional properties of the biological material, from the cellular level down to single molecules, owes its success to the development of suitable high-sensitivity platforms to image the biomaterial and analyze its response to specific stimuli. Imaging has indeed reached molecular capabilities,...

A versatile microfluidic, multichamber cell culture and analysis system with an integrated electrode array and potentiostat suitable for electrochemical detection and microscopic imaging is presented in this paper. The system, which allows on-line electrode cleaning and modification, was developed for real-time monitoring of cellular dynamics, exem...

A versatile 4-channel bipotentiostat system for biochemical sensing is presented. A 1pA current resolution and 8 kHz bandwidth are suited for amperometric detection of neurotransmitters released by cells, monitored in a smart microfluidic culture chamber. Multiple electrochemical measurements can be carried out on arrays of microelectrodes. Key des...