Marco Cristancho

centro de bioinformatíca y biología computacional · Research Department

Coffee leaf rust is the most damaging disease for coffee cultivation around the world. It is caused by a fungal pathogen, Hemileia vastatrix (Hva), belonging to the phylum Basidiomycota. Coffee leaf rust causes significant yield losses and increases costs related to its control, with evaluated losses of USD 1–2 billion annually. It attacks both the...

Microorganisms represent nearly 90% of ocean biomass and are fundamental for the functioning and health of marine ecosystems due to their integral contribution to biogeochemical cycles and biological processes. In marine environments, microorganisms exist as microbial communities in the water column, benthonic substrates, and macroorganisms, where...

Nunca antes se han tenido tantos datos de secuenciación disponibles y la posibilidad de contar con tecnologías que se actualizan constantemente, que permiten estudiar de forma masiva y simultánea cientos de especies para diferentes objetivos, entre los cuales se destacan los estudios de taxonomía molecular, evolución y la producción de compuestos p...

Coffee rust is a leaf disease caused by the fungus, Hemileia vastatrix. Coffee rust epidemics, with intensities higher than previously observed, have affected a number of countries including: Colombia, from 2008 to 2011; Central America and Mexico, in 2012–13; and Peru and Ecuador in 2013. There are many contributing factors to the onset of these e...

Coffee supports the livelihood of millions of families in producing countries, and enriches the life of millions of coffee consumers worldwide. However, the most globally devastating insect pest of coffee: Hypothenemus hampei, the Coffee Berry Borer (CBB), threatens the sustainability of the coffee industry in the context of climate change, as incr...

Coffee leaf rust caused by the fungus Hemileia vastatrix is the most damaging disease to coffee worldwide. The pathogen has recently appeared in multiple outbreaks in coffee producing countries resulting in significant yield losses and increases in costs related to its control. New races/isolates are constantly emerging as evidenced by the presence...

The coffee rust, caused by the fungus Hemileia vastatrix, is the most serious disease of this crop worldwide. In Colombia the pathogen causes a reduction in production of up to 30% in susceptible varieties of Coffea arabica, if not controlled mild epidemics can occur and complete crop losses in strong epidemics. We applied genomics to study the evo...

This volume compiles accepted contributions for the 2nd Edition of the Colombian Computational Biology and Bioinformatics Congress CCBCOL, after a rigorous review process in which 54 papers were accepted for publication from 119 submitted contributions. Bioinformatics and Computational Biology are areas of knowledge that have emerged due to advance...

Coffee leaf rust, caused by the fungus Hemileia vastatrix, is the most damaging disease of coffee worldwide. The pathogen has caused yield reductions of up to 30% in susceptible varieties of Coffea arabica in recent epidemics associated with climate change in Colombia and several Central American countries. We have sequenced de novo the genome and...

Since 2008, Colombia has been experiencing an epidemic of the coffee rust Hemileia vastatrix. The altitude range of the disease has expanded, and nursery and young plants that were usually not attacked by the disease are now significantly affected. To determine whether this new epidemic has been caused by a new pathogenic isolate, the molecular div...

In Colombia, coffee cultivation covers 850,000 ha, of which 41% comprises cultivars of Coffea arabica that are susceptible to leaf rust (CLR) caused by Hemileia vastatrix Berk. et Br.CLR, first recorded in Colombia in 1983, is the most…

Background / Purpose: The fungi Hemileia vastatrix is the causal agent of coffee leaf rust. Here, we studied its genome and searched for pathogenic factors. Main conclusion: We assembled the coffee leaf rust genome and assessed its quality. Although some work is needed to complete the genome, this draft genome is a useful starting place in the...

The coffee berry borer (CBB; Hypothenemus hampei) is a major pest of coffee responsible for significant crop losses worldwide. The entomopathogenic fungus Beauveria bassiana represents a natural means of controlling this insect pest; however, little is known concerning the molecular determinants that contribute to the virulence of the fungus toward...

The genomes of nine isolates of Hemileia vastatrix , the causal agent of coffee leaf rust were sequenced by Illumina and 454. Quality control, cleaning and de novo assemblies of data were performed. Since isolates were obtained from the field and it is not possible to produce axenic cultures of H. vastatrix , MEGAN software was used to evaluate con...

The coffee berry borer (CBB) is the most prevalent pest of coffee plantations. Within the Coffea genus, C. arabica is susceptible to CBB and C. liberica shows a lower susceptibility. Two EST libraries were constructed from the total RNA of C. arabica and C. liberica fruits artificially infested with CBBs for 24 h. Using 6000 clones sequenced per li...

Los principales y más revolucionarios avances de la biología en este siglo se han derivado de la información proveniente de genomas completos de diferentes organismos. Los descubrimientos que se derivan de la genómica están generando un nuevo paradigma en la biología, sustituyendo la era de la biología centrada en los genes por aquella centrada en...

The main, most revolutionary advances in biology during this century have arisen from information being provided from revealing different organisms’ complete genomes. The discoveries deriving from genomics are leading to a new paradigm in biology, the era of gene-centred biology being substituted for that centred on genomes. Such new concepts areas...

[This corrects the article on p. e1000535 in vol. 5, PMID: 19876381.].

High-throughput techniques are somewhat restricted in developing countries. However, computational resources have evolved in recent years to become available to the general public, with greater ability to solve intense computational problems at low cost. Therefore, the vast amount of information that is currently being generated and the need for fi...

High-throughput techniques are somewhat restricted in developing countries. However, computational resources have evolved in recent years to become available to the general public, with greater ability to solve intense computational problems at low cost. Therefore, the vast amount of information that is currently being generated and the need for fi...

This study reports the implementation of three strategies for the development of genetic markers and their evaluation in both progenitors of an F2 population used for the construction of a genetic map of Coffea arabica. The strategies were Cleaved Amplified Polymorphic Sequences (CAPS), Single Strand Conformational Polymorphism (SSCP), and sequence...

Simple sequence repeat (microsatellite) loci in coffee were identified in clones isolated from enriched andrandom genomic libraries. It was shown that coffee is a plant species with low microsatellite frequency. However, the averagedistance between two loci, estimated at 127kb for poly (AG), is one of the shortest of all plant genomes. In contrast,...

The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 2...

We have implemented a web-based Bioinformatics platform that functions as a genomics information resource for coffee and other organisms studied at the Colombia National Coffee Research Center -CENICAFE. The Bioinformatics platform includes a Laboratory Integrated Management System (LIMS), the implementation of wEMBOSS, home-developed perl tools fo...

The genetic diversity of 50 wild and semi-wild accessions of the Coffea arabica L. germplasm collection, gathered by the FAO and ORSTOM missions to Ethiopia, and maintained in Colombia by CENICAFE, was evaluated with RAPD markers. The evaluation was carried out in two phases: In phase one, the polymorphism of 8 Ethiopian accessions of different geo...

Ardila: Sistema de información para el manejo de datos moleculares en café: 1. Desarrollo y uso de herramientas. Rev. Acad. Colomb. Cienc. 32(124): 317-324, 2008. ISSN 0370-3908. Con la gran cantidad de información que en la actualidad se obtiene en los proyectos de estudio de genomas, es necesario crear una estrategia para que los datos sean almac...

Sistema de información para el manejo de datos moleculares en café: II. desarrollo de bases de datos. Rev. Acad. Colomb. Cienc. 32(124): 325-330, 2008. ISSN 0370-3908. Cenicafé ha realizado el desarrollo de una plataforma de Bioinformática para el almacenamien-to, análisis y fácil accesibilidad de los datos del proyecto de estudio del genoma del ca...

Coffee Berry Borer (CBB), Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae) is the most important insect pest of coffee worldwide. No sources of natural resistance have been identified in Coffea species. However, Coffea liberica has shown some level of antibiosis against the insect. In order to understand the interactions between C. arabica...

RESUMEN ESCOBAR O., C.; CRISTANCHO A., M. Estudio de metodologías para la conservación de urediniosporas de la roya del cafeto. Cenicafé 58(4): 324-332.2007. La roya anaranjada del cafeto (Hemileia vastatrix) es un parásito biotrófico que no ha sido posible cultivar in vitro, lo cual obliga al mantenimiento de aislamientos puros mediante ciclos con...