Marcio CostaUniversité de Montréal | UdeM · Faculty of Veterinary Medicine
Marcio Costa
DVM, PhD
About
70
Publications
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Introduction
Marcio Costa currently works at the Department of Veterinary Biomedical Sciences, University of Montreal. Marcio does research in intestinal microbiota of domestic animals.
Additional affiliations
April 2017 - January 2019
September 2010 - November 2013
July 2007 - August 2010
Publications
Publications (70)
Objectives
Clostridioides difficile (formerly Clostridium difficile) is associated with colitis in foals and mature horses. C. difficile exposes specific phosphorylated polysaccharides (PSs), named PS-I, PS-II and PS-III. These cell-surface PSs are potential vaccine targets, especially the hexasaccharide phosphate PS-II, that has been found in all...
Calves born on Eastern Canadian dairy farms that are not kept in the herds are traditionally sold through auction markets and are raised for meat purposes such as veal calves. Since February 2020, a new Canadian federal regulation has forbidden calves <9 d old to be sold through auction markets. However, in the absence of a real-time birth registry...
Alterations in the gastrointestinal microbiota after antimicrobial therapy in horses can result in loss of colonization resistance and changes in bacterial metabolic function. It is hypothesized that these changes facilitate gastrointestinal inflammation, pathogen expansion and the development of diarrhea. The objectives of this study were to deter...
Abstract Background Intestinal health remains a key factor in animal production because it is essential for digestion, absorption and bacterial fermentation. Feed additives have been used to attenuate the weaning stress such as Zinc Oxide (ZnO) and benzoic acid (C7H6O2). The objective of this study was to evaluate the impact of of benzoic acid and...
The purpose of this study was to characterize the fecal microbiota of horses with acute and chronic diarrhea before and after fecal microbiota transplantation (FMT). Six client-owned horses with acute and chronic diarrhea received FMT from 2 healthy donor horses. Microbiota analysis using next-generation sequencing was performed on fecal samples co...
All current studies have used Illumina short-read sequencing to characterize the equine intestinal microbiota. Long-read sequencing can classify bacteria at the species level. The objectives of this study were to characterize the gut microbiota of horses at the species level before and after trimethoprim sulfadiazine (TMS) administration and to com...
This study investigated the relationship between the bacterial communities of bulk tank milk (BTM) and water used in Brazilian dairy farms. We also explored the association between BTM microbiota and its quality parameters (i.e., somatic cell count [SCC] and total bacteria count [TBC]). Water and BTM samples were collected twice for high throughput...
The aim of the present study was to characterize the bacterial microbiota of anal sacs in healthy dogs using NGS. Swabs were used to sample the rectum and secretions from each anal sac in 15 healthy dogs. DNA was extracted from swabs and the V4 hypervariable region of the 16S rRNA gene was amplified and sequenced with Illumina MiSeq. Overall, 14 di...
The Canadian Food Inspection Agency is developing an Establishment-based Risk Assessment (ERA) model for commercial and on-farm mills involved in the manufacture, storage, packaging, labeling, or distribution of livestock feed (ERA–Feed Mill model). This model will help inform the allocation of inspection resources on the basis of feed safety risk,...
Le Québec produit 80% des veaux lourds au Canada. Les élevages de veaux lourds présentent: un taux de mortalité de 8-10% et un taux de morbidité jusqu'à 60%. L'objectif de l'étude était l'évaluation des caractéristiques et de la santé des veaux vendus lors de la vente aux encans. Deux visites aux encans de Saint-Hyacinthe et de Saint Isidore ont ét...
The ruminal microbiota is a complex and rich community that is stable and difficult to manipulate. Transfaunation is considered a useful method to re-stablish a dysbiotic community, such as in cases of ruminal acidosis. The objective of this study was to evaluate the consequences of transfaunation on the ruminal bacteria of healthy sheep receiving...
The safety of livestock feed is recognized worldwide as a prerequisite for the protection of animal and human health. In this context, the Canadian Food Inspection Agency is developing an Establishment-based Risk Assessment model for livestock feed mills (ERA-Feed Mill) to assist in the allocation of inspection resources based on feed safety risk....
Simple Summary: Acute diarrhea (colitis) is a major problem in adult horses and the role of the intestinal bacteria (microbiota) is still poorly understood in this species. The aim of this study was to compare the mucosal and luminal content microbiota of the cecum and colon of healthy and diarrheic horses. We concluded that microbial dysbiosis (ch...
Background:
Gastrointestinal microbiota can be influenced by several factors, including diet and systemic inflammation, and in turn could act as a modulator of the allergic response. Fecal microbiota of horses with asthma has not been described.
Hypothesis/objectives:
Analyze the bacterial fecal microbiota of horses with and without asthma under...
New insights into the host-microbiota relationship have recently emerged with the advancement of molecular technologies such as next-generation sequencing. This article presents the current knowledge regarding the interaction between bacteria and the immune system of the gut, the uterus, and the mammary gland of cattle.
Understanding gut microbiota similarities and differences across breeds in horses has the potential to advance approaches aimed at personalized microbial modifications, particularly those involved in improving sport athletic performance. Here, we explore whether faecal microbiota composition based on faecal 16S ribosomal RNA gene sequencing varies...
There has been a marked increase in interest regarding complex microbial populations in recent years. The methodology used for microbial assessment has drastically changed over the last two decades and continues to advance at a rapid pace. Culture-based studies have been superseded by those based upon molecular methods, which have been largely used...
Objectives
To use next generation sequencing to characterize the microbiota of horses during healing of skin wounds in two anatomical locations (body and limb) known to present different healing patterns; and to investigate the impact of bandaging on bacterial communities of skin wounds located on the limbs of horses.
Methods
Full-thickness skin w...
This article provides readers with the basic concepts necessary to understand studies using recent molecular methods performed in intestinal microbiome assessment, with special emphasis on the high throughput sequencing. This review also summarizes the current knowledge on this topic and discusses future insights on the interaction between the inte...
Background:
Diarrhea associated with parvovirus infection is common in dogs. Supportive care is the mainstay of treatment, but recovery may be prolonged and mortality rate can be high. Modification of the intestinal bacterial microbiota has been promising in human and veterinary medicine as an adjunctive treatment of various enteric diseases.
Obj...
Aflatoxin B1 (AFB1), a mycotoxin found in food and feed, exerts harmful effects on humans and animals. The liver is the earliest target of AFB1, and its effects have been evaluated in animal models exposed to acute or chronic doses. Considering the possibility of sporadic ingestion of AFB1-contaminated food, this study investigated the impact of a...
The liver and the gastrointestinal tract are the earliest target for the harmful effects of aflatoxin B1 (AFB1). This study investigated the impact of a single oral administration of AFB1 (663 μg of AFB1/kg of body weight) on the gut microbial community of C57Bl/6 mice. Sequencing of the V4 region of the 16S rRNA gene was performed with an Illumina...
Several factors are known to affect the intestinal microbiota of cattle. However, how these changes occur over time is poorly understood. This study aimed to investigate the consequences of entrance into a feedlot and the effects of virginiamycin used as a growth promoter on the bovine fecal microbiota. Two batches of beef cattle (B1, n = 50 and B2...
Background:
Neonatal diarrhea accounts for more than 50% of total deaths in dairy calves. Few population-based studies of cattle have investigated how the microbiota is impacted during diarrhea.
Objectives:
To characterize the fecal microbiota and predict the functional potential of the microbial communities in healthy and diarrheic calves.
Met...
Antimicrobials are sometimes given to food animals at low doses in order to promote faster growth. However, the mechanisms by which those drugs improve performance are not fully understood. This study aimed to investigate the impact of zinc bacitracin (55g/ton), enramycin (10g/ton); halquinol® (30g/ton); virginiamycin (16,5g/ton) and avilamycin (10...
Recent studies performed in humans and rats have reported that exercise can alter the intestinal microbiota. Athletic horses perform intense exercise regularly, but studies characterizing horse microbiome during aerobic conditioning programs are still limited. Evidence has indicated that this microbial community is involved in the metabolic homeost...
Background:
Duodenitis-proximal jejunitis (DPJ) is an acute sporadic gastrointestinal disorder of horses of unknown cause.
Hypothesis/objectives:
We hypothesize that Clostridium difficile toxins are involved in the pathogenesis of DPJ in horses. The objective of this study was to determine whether experimentally delivered C. difficile toxins cau...
O Transplante de Microbiota Fecal (TMF) tem sido utilizado com altas taixas de sucesso em humanos. A gastrenterite hemorrágica está entre os problemas mais comuns que acometem os cães. Assim sendo, o objetivo desse trabalho foi investigar o efeito do TMF na recuperação clínica de cães com gastrenterite hemorrágica. No presente trabalho foram avalia...
O Transplante de Microbiota Fecal (TMF) tem sido utilizado com altas taxas de sucesso em humanos. A gastrenterite hemorrágica está entre os problemas mais comuns que acometem os cães. Assim sendo, o objetivo desse trabalho foi testar a segurança de um protocolo de TMF e sua eficácia na recuperação clínica de cães com gastrenterite hemorrágica. Fora...
As bactérias intestinais formam um ecossistema complexo e exercem enorme importância na manutenção da homeostase do hospedeiro. Por vezes, intervenções graves podem levar à degradação deste ecossistema, como por exemplo, através de doenças e/ou pelo uso de antibióticos. Este relato tem por objetivo demonstrar o uso do Transplante de Microbiota Feca...
Reasons for performing study:
The intestinal microbiota is a complex polymicrobial ecosystem that exerts extremely important roles in the development and maintenance of health. Recently, as new sequencing technologies have become more available, there has been a revolution in the understanding of the equine intestinal microbiota. However, studies...
The objective of this study was to characterize H1N1 and H1N2 influenza A virus isolates
detected during outbreaks of respiratory disease in pig herds in Ontario (Canada) in 2012.
Six influenza viruses were included in analysis using full genome sequencing based on the
454 platform. In five H1N1 isolates, all eight segments were genetically related...
The advance of new sequencing technologies has allowed more comprehensive characterization of complex microbial communities, including the ones inhabiting the intestinal tract. The presence of extreme environmental filters, such as low pH, digestive enzymes and anaerobic conditions along the tract, acts on the selection of unique bacteria in each c...
Background
The intestinal tract is a rich and complex environment and its microbiota has been shown to have an important role in health and disease in the host. Several factors can cause disruption of the normal intestinal microbiota, including antimicrobial therapy, which is an important cause of diarrhea in horses. This study aimed to characteriz...
Background
Data about molecular diversity of commonly circulating type A influenza viruses in Ontario swine are scarce. Yet, this information is essential for surveillance of animal and public health, vaccine updates, and for understanding virus evolution and its large-scale spread.Methods
The study population consisted of 21 swine herds with clini...
The healthy feline oral cavity harbours a rich assemblage of microorganisms, which have not previously been well characterized using modern sequencing technology. The goal of this study was to accurately describe the oral microbiota of 11 healthy cats using next-generation sequencing.
Sequencing generated a total of 10,177 operational taxonomic uni...
IntroductionRepeated monitoring of faecal pH may be useful in detecting the effects of exercise and conditioning on the fermentative activity within the large intestine. This study investigated the effect of a maximal incremental exercise test (MIET) and aerobic conditioning on the faecal pH of untrained horses.Methods
Fourteen equines were randoml...
Previous studies showed that Nelore calves' active production of immunoglobulins is delayed compared to Canchim and Limousin calves, characterizing a possible physiologic difference between taurine and Zebu calves. In this study we compared serum proteins of Nelore and Holstein calves from birth to six months old. Blood samples were taken from 50 h...
Twenty-six isolates of Clostridium perfringens of different MLST types from chickens with necrotic enteritis (NE) (15 netB-positive) or from healthy chickens (6 netB-positive, 5 netB-negative) were found to contain 1-4 large plasmids, with most netB-positive isolates containing 3 large and variably sized plasmids which were more numerous and larger...
Amino acid alignments of proteins encoded by different C. perfringens plasmids. Plasmid names and their respective orf number (plasmid name orf#) are described for each protein. Identical residues (*), conservative amino acid substitutions (:), and semi-conservative amino acid substitutions (.) are shown below the aligned sequences. (MUSCLE −3.7)....
PFGE analyses of plasmids from transconjugants C. perfringens strains. Agarose plugs containing DNA from each specified isolate were digested with NotI and subjected to PFGE and staining with ethidium bromide. Lines indicate: CW504 recipient strain (plasmid free); T98 (transconjugant carrying the plasmid pCpb2); T117 (transconjugant carrying three...
List of primers. (A) Primers used for PCR DIG labelling and mutation (B) Primers used for overlapping PCR reactions of the three Pathogenicity loci characteristic of necrotic enteritis C. perfringens isolates.
(DOCX)
Comparison of NE C. perfringens plasmids. (A) Comparison of coding sequences pNetB-NE10 and pJIR3535 NE C. perfringens plasmids by means of BLASTn analyses. Open reading frames are labeled according to the annotation of plasmid pNetB-NE10 (B) Comparison of open reading frames pCpb2-CP1 and pJIR3844 NE C. perfringens plasmids by means of BLASTn anal...
Overlapping PCR analysis
of NE locus in C. perfringens. PCR reactions were performed using DNA from C. perfringens strains described on Table 1. Healthy and NE C. perfringens isolates H26, H34, NE04, NE09, NE10, NE14, NE20, NE23, NE28, NE30, NE42, respectively. Genetic organization of NE loci. (A) Overlapping PCR analysis of NE locus 1. (B) Overlap...
Repeats found on the upstream region of parM gene. Possible tandem repeats found on the upstream region of parM gene next to rep gene from C. perfringens plasmids using etandem (http://emboss.bioinformatics.nl/cgi-bin/emboss/etandem).
(DOCX)
PFGE and Southern blot analyses of plasmids from healthy C. perfringens poultry strains. (A) PFGE analyses of plasmids from healthy C. perfringens poultry strains. Agarose plugs containing DNA from each specified isolate were digested with NotI and subjected to PFGE and staining with ethidium bromide. See Table1 and 2 for isolate features. Line num...
Efficient characterization of the canine oral microbiome is critical for understanding the role of oral bacteria in health and for providing insight into early diagnosis and treatment strategies against periodontal disease. To date, characterization has been limited to cloning-based sequencing and conventional culture-based studies, which generally...
Metagenomic analysis is a very rich area for understanding the microbiology of organisms. Once the data has been assembled mathematical and statistical methods can be applied providing insights into biological properties that created the data in the first place. The foundations however, require some knowledge of microbiology which is not usually pa...
The intestinal tract houses one of the richest and most complex microbial populations on the planet, and plays a critical role in health and a wide range of diseases. Limited studies using new sequencing technologies in horses are available. The objective of this study was to characterize the fecal microbiome of healthy horses and to compare the fe...
The equine intestinal tract contains a complex microbial population (microbiota) that plays an important role in health and disease. Despite the undeniable importance of a 'normal' microbiota, understanding of the composition and function of this population is currently limited. As methods to characterize the microbiota and its genetic makeup (the...
The presence of indistinguishable strains of Clostridium difficile in humans, food animals and food, as well as the apparent emergence of the food-animal-associated ribotype 078/toxinotype V as a cause of community-associated C. difficile infection have created concerns about the potential for foodborne infection. While studies have reported C. dif...
This study demonstrated that bovine herpesvirus 5 (BoHV)-5 infected calves can develop encephalitis and remain asymptomatic. Seven calves were infected intranasally and monitored for 30 days. Cerebrospinal fluid (CSF) analysis was performed from the onset of neurological signs. Multiple sections of brain and the trigeminal ganglion were submitted t...
Concern has been raised about the potential for Clostridium difficile to be a bovine and foodborne pathogen, yet limited study has been performed in cattle, and none in veal calves. This study evaluated the epidemiology and microbiology of C. difficile on one veal farm. Rectal swabs were obtained from calves within 48 h of arrival and at one, 17 an...
Clostridium difficile is an important gastrointestinal pathogen of humans and animals. It has been isolated from various foods, including meat and ready-to-eat salads, and concern has been expressed regarding food as a possible source of human C. difficile infection (CDI).
We sought to isolate C. difficile from a variety of vegetables obtained from...
The objective of this study was to evaluate the reproductive performance of ewes mated in the spring when given nutritional supplements to enhance energy levels. Ewes were assessed for weight and body condition at the beginning and end of flushing periods. Lambing rates and rates of proliferation were also evaluated. Crossbred woolly ewes (n = 46),...
Evaluation and observation was carried out with 24 equidae put on oxytetracycline hydrochloride medication, a drug incriminated as potential cause of severe colitis with diarrhea manifestations for this species. The animals of this study were natural carriers of affections that required antimicrobial use. During 4 to 19 uninterrupted days intraveno...
Com o objetivo de investigar alguns aspectos relacionados à transferência de imunidade passiva em bovinos de corte, foram selecionados 90 bezerros aparentemente sadios, 45 da raça Nelore e 45 da raça Limousin, distribuídos em 3 grupos (com 15 bezerros cada) de acordo com o número de parições de suas mães: primeira cria; segunda cria; e terceira ou...
To study some defence mechanisms of beef calves kept on range conditions, 90 healthy calves, 45 Nelore and 45 Limousin, were bled at 24 to 36 hours of life, 15, 30, 60, 90 and, 120 days. Leukogram and neutrophil oxidative burst through the non-stimulated and the stimulated NBT tests were determined. The parasitemia with Anaplasma marginale, Babesia...
To study the passive transfer of immunity, 90 healthy calves, 45 Nelore and 45 Limousin, were grouped (n=15) according to their dam's parity: first, second or third or more calvings. Blood samples were draw from each calf with 24 to 36 hours of life and 15, 30, 60, 90, and 120 days. The total serum (TSP) and plasma (TPP) proteins, gamaglutamiltrans...
Evaluation and observation was carried out with 24 equidae put on oxytetracycline hydrochloride medication, a drug incriminated as potential cause of severe colitis with diarrhea manifestations for this species. The animals of this study were natural carriers of affections that required antimicrobial use. During 4 to 19 uninterrupted days intraveno...
Projects
Projects (3)
1-To compare the bacterial signature of the vaginal and uterine ecosystem of high performance cows with and without the different post uterine diseases.
2- To compare the profile of local mediators of inflammation in high performance cows with and without different post uterine diseases.
The journal Animals is currently inviting submissions for a Special Issue entitled "Equine Microbiota"
The objective of this special issue is to collect studies evaluating the importance of the microbiota in horses, including not only the intestinal, but also ocular, cutaneous, vaginal, respiratory tract and other microbial communities. Thus, we invite original research papers and reviews bringing new relevant insights into the equine microbiota.
The submission deadline is 31 Dec 2020.
https://www.mdpi.com/journal/animals/special_issues/Equine_Microbiota