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Marcelo F Gouveia Nogueira

Marcelo F Gouveia Nogueira
São Paulo State University (UNESP) · Department of Biological Science

PhD, MSc, DVM, Associate Professor
Associate Professor at School of Sciences and Languages in campus of Assis (São Paulo State University - UNESP).

About

158
Publications
28,847
Reads
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2,975
Citations
Citations since 2017
90 Research Items
1551 Citations
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2017201820192020202120222023050100150200250300
2017201820192020202120222023050100150200250300
Introduction
As Associate Professor, Marcelo F Gouveia Nogueira currently works at the Department of Biological Sciences, Campus Assis, São Paulo State University (UNESP). Marcelo does research in Developmental Biology, Cell Biology and Molecular Biology. His last project was 'GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle'.
Additional affiliations
May 2010 - present
São Paulo State University (UNESP)
Position
  • Professor (Associate)
Education
January 1984 - December 1988
São Paulo State University
Field of study
  • Veterinary medicine

Publications

Publications (158)
Article
Full-text available
Several opportunities for embryo development, stem cell maintenance, cell fate, and differentiation have emerged using induced pluripotent stem cells (iPSCs). However, the difficulty in comparing bovine iPSCs (biPSCs) with embryonic stem cells (ESCs) was a challenge for many years. Here, we reprogrammed fetal fibroblasts by transient expression of...
Article
Full-text available
Several opportunities for embryo development, stem cell maintenance, cell fate, and differentiation have emerged using induced pluripotent stem cells (iPSCs). However, the difficulty in comparing bovine iPSCs (biPSCs) with embryonic stem cells (ESCs) was a challenge for many years. Here, we reprogrammed fetal fibroblasts by transient expression of...
Conference Paper
Study question Is it possible to estimate blastocyst ploidy with an artificial intelligence (AI) algorithm built using embryo morphokinetics data? Summary answer AI was able to estimate blastocysts status in euploid or aneuploid with an accuracy of 0.99 (training) and 0.71 and 0.70 AUC (blind-test), respectively. What is known already Morphokinet...
Article
Full-text available
Giant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes or embryos during in vitro culture can induce the opening of pores in the membrane of these vesi...
Article
Full-text available
The aim of this study was to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in co-culture with in vitro-produced bovine embryos (IVPEs). The stability of five concentrations of MLVs (1.0, 1.25, 1.5, 1.75, and 2.0 mM) produced using ultrapure water or embryonic culture medium with 24 or 48...
Article
This is a feasibility study aimed to determine the effectiveness of a culture media designed to enhance embryonic cryopreservation based on laboratory-accessed quality phenotypes of in vitro produced bovine embryos. Recovered bovine oocytes were matured and fertilized in vitro and the presumptive zygotes that were obtained were randomly distributed...
Article
Full-text available
Despite the use of new techniques on embryo selection and the presence of equipment on the market, such as EmbryoScope® and Geri®, which help in the evaluation of embryo quality, there is still a subjectivity between the embryologist’s classifications, which are subjected to inter- and intra-observer variability, therefore compromising the successf...
Preprint
Full-text available
OBJECTIVE To study the application of image processing for segmentation of blastocysts images and extraction of potential variables for prediction of embryo fitness. DESIGN Retrospective study. SETTING Single reproductive medical center. IVI-RMA (Valencia, Spain) between 2017 and 2019. PATIENTS An initial dataset including 353 images from Embryo...
Article
The use of different sires influences in vitro embryo production (IVP) outcome. Paternal effects 26 are observed from the first cleavages until after embryonic genome activation (EGA). Little is 27 known about the mechanisms that promote in vitro fertility differences, even less about the 28 consequences on embryo development. Therefore, this st...
Article
Full-text available
The event of cellular reprogramming into pluripotency is influenced by several factors, such as in vitro culture conditions (e.g., culture medium and oxygen concentration). Herein, bovine iPSCs (biPSCs) were generated in different levels of oxygen tension (5% or 20% of oxygen) and supplementation (bFGF or bFGF + LIF + 2i—bFL2i) to evaluate the effi...
Article
Antral follicle count (AFC) was phenotypically and genetically evaluated in a Bos taurus cow population. A total of 257 Aberdeen Angus cows underwent an ovarian ultrasound evaluation and hormonal synchronization for timed artificial insemination (TAI). These animals were between 40 and 70 d postpartum with a body score condition (BSC) between 2.0 a...
Article
An artificial intelligence model was designed using proteomic and morphological data from blastocysts. The algorithm, based on artificial neural networks, is capable of discriminating with high accuracy between euploid embryos that lead to a live birth and those which do not. ABSTRACT Research question: The study aimed to develop an artificial inte...
Article
Full-text available
In several species, oocyte and embryo competence are improved by the addition of endoplasmic reticulum (ER) stress inhibitors to in vitro maturation (IVM) medium and/or in vitro culture medium. This study aimed to evaluate the effects of three concentrations of tauroursodeoxycholic acid (TUDCA; 50 μM, 200 μM, and 1,000 μM), a chemical chaperone for...
Patent
INPI CARTA PATENTE Nº BR 102012031953-5 O INSTITUTO NACIONAL DA PROPRIEDADE INDUSTRIAL concede a presente PATENTE DE INVENÇÃO, que outorga ao seu titular a propriedade da invenção caracterizada neste título, em todo o território nacional, garantindo os direitos dela decorrentes, previstos na legislação em vigor. (21) Número do Depósito: BR
Patent
Dito método para encapsulamento de antioxidantes acrescenta as vesículas unilamelares gigantes - GUVs - contendo antioxidantes em sistemas de cultivo in vitro de gametas ou embriões de mamíferos, para a liberação dos antioxidantes para combater o estresse oxidativo característico do ambiente de cultivo in vitro.
Patent
A presente invenção refere-se ao método de geração de embriões para desenvolver e multiplicar diferentes linhagens celulares e/ou indivíduos geneticamente iguais a partir da incorporação de células pluripotentes em trofectoderme. O presente método gera embriões quiméricos (preferencialmente mamíferos, incluindo de seres humanos), homólogos (contend...
Article
Full-text available
We evaluated the effect of the antral follicle count (AFC) on ovarian follicular dynamics, pregnancy rates, progesterone concentrations, and transcriptional patterns of genes in Nelore cattle (Bos taurus indicus) after a timed artificial insemination (TAI) programme. Cows were separated based on the AFC, and those with a high AFC showed a larger (P...
Article
The cryosurvival of embryos is a complex process involving dynamic and integrated morphological, functional, and molecular changes. Here, we evaluated the tran-scriptional profiling of bovine embryos possessing high and low cryotolerance (HC and LC, respectively) by assessing the resumption of development. Embryos were produced in vitro (N = 1137)...
Article
Research Question To develop an Artificial Intelligence (AI) model based on Artificial Neural Networks (ANNs) to predict the likelihood of achieving a live birth (LB) by using the proteomic profile of spent culture media and morphological data from time-lapse images. Design This retrospective cohort study included 212 patients who underwent single...
Article
Full-text available
Over the past years, the assisted reproductive technologies (ARTs) have been accompanied by constant innovations. For instance, intracytoplasmic sperm injection (ICSI), time-lapse monitoring of the embryonic morphokinetics, and PGS are innovative techniques that increased the success of the ART. In the same trend, the use of artificial intelligence...
Article
Full-text available
Previous studies have discussed the importance of an optimal range of metabolic activity during preimplantation development. To avoid factors than can trigger an undesirable trajectory, it is important to learn how nutrients and metabolites interact to help launching the correct developmental program of the embryo, and how much the in vitro culture...
Article
Full-text available
In many cell types, epigenetic changes are partially regulated by the availability of metabolites involved in the activity of chromatin-modifying enzymes. Even so, the association between metabolism and the typical epigenetic reprogramming that occurs during preimplantation embryo development remains poorly understood. In this work, we explore the...
Article
Full-text available
Autophagy is a physiological mechanism that can be activated under stress conditions. However, the role of autophagy during oocyte maturation has been poorly investigated. Therefore, this study characterized the role of autophagy on developmental competence and gene expression of bovine oocytes exposed to heat shock (HS). Cumulus-oocyte-complexes (...
Article
C-type natriuretic peptide (CNP) and its natriuretic peptide receptors subtype 2 (NPR2) are essential for the maintenance of oocyte meiotic arrest in different species. Extracellular vesicles (EVs) in bovine follicular fluid (FF) are important for cell communication within the ovarian follicle. This study investigated the involvement of EVs from FF...
Article
Full-text available
Abstract: Reproductive efficiency plays a major role in the long-term sustainability of livestock industries and can be improved through genetic and genomic selection. This study aimed to estimate genetic parameters (heritability and genetic correlation) and identify genomic regions and candidate genes associated with anti-Müllerian hormone levels...
Article
Full-text available
Based on growing demand for assisted reproduction technology, improved predictive models are required to optimize in vitro fertilization/intracytoplasmatic sperm injection strategies, prioritizing single embryo transfer. There are still several obstacles to overcome for the purpose of improving assisted reproductive success, such as intra-and inter...
Article
Sample types such as those from reproductive systems often yield scarce material, which limits RT-qPCR analysis to only a few targets. Recently developed high-throughput systems can potentially change this scenario, however, the nanoliter scale of such platforms requires extra processing, e.g., preamplification, which needs to be defined through ob...
Conference Paper
Full-text available
After the emergence of induced cell reprogramming, achieved through the addition of Yamanaka transcription factors (Oct4, Sox2, Klf4, and cMyc; OSKM) to somatic cells, the number of studies regarding induction and maintenance of pluripotency has increased greatly. The success of bovine iPSCs (biPSCs) was first described by Summer et al. (2011 J. An...
Chapter
We develop an online graphical and intuitive interface connected to a server aiming to facilitate access to professionals worldwide that face problems with bovine blastocysts classification. The interface Blasto3Q (3Q is referred to the three qualities of the blastocyst grading) contains a description of 24 variables that are extracted from the ima...
Article
The effectiveness of the use of natriuretic peptide C (NPPC) in the blocking of meiosis has already been proven in several species. However, there are no reports on the use of NPPC in the activation of metabolic processes in embryos. Whereas modulations of cAMP concentrations alter the lipid metabolism of bovine oocytes, the present study aims to e...
Article
Studies have shown that the use of equine chorionic gonadotropin (eCG), which binds both follicle stimulating hormone (FSH) and luteinizing hormone (LH) receptors, could modify the female reproductive tract. We, thus, aimed to quantify the messenger RNA (mRNA) abundance of genes related to cumulus‐oocyte complexes (COCs) and embryo quality in Nelor...
Article
Full-text available
In this study, we evaluated the modulation effect of long-chain Acyl-CoA synthetase during early embryo development. Bovine embryos were cultured in four groups: positive modulation (ACS+) with GW3965 hydrochloride, negative modulation (ACS-) with Triacsin C, association of both modulators (ACS±), and control. Embryo development rates were not alte...
Article
Insulin‐like growth factor 1 (IGF‐1) activity is established by the regulation of IGF binding protein activity, which blocks IGF‐1 functions, whereas pregnancy‐associated plasma protein‐A (PAPP‐A) improves IGF‐1 bioavailability and facilitates binding to IGF receptors. To further extend our understanding of the effect of exogenous PAPP‐A on bovine...
Article
The effect of heat stress (HS) on cattle reproduction is deleterious with respect to ovarian follicular development and oocyte quality. The objective of this study was to investigate the effect of follicular fluid extracellular vesicles (EVs) obtained from cows maintained in thermoneutral (TN) or HS conditions on in vitro oocyte maturation. Nonlact...
Article
Full-text available
Purpose Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently “primed” for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured,...
Article
The objective of this study was to evaluate the effects of forskolin supplementation of the culture medium on the development, lipid content, and transcript levels genes of in vitro-produced bovine embryos. Ovaries (n = 420) from Nelore cows were obtained from the slaughterhouse. On day 5 (D5) of in vitro culture, the embryos in the experimental gr...
Article
The epidermal growth factor receptor (EGFR) pathway is directly involved in oocyte meiotic resumption induced by a gonadotropic stimulus. Here, we used an EGFR inhibitor (AG1478) to inhibit spontaneous meiosis resumption in bovine oocytes (EGFR‐ group) during 8 hours prematuration and assessed the competence of such oocytes for embryonic developmen...
Article
Full-text available
In this study, we developed an online graphical and intuitive interface connected to a server aiming to facilitate professional access worldwide to those facing problems with bovine blastocysts classification. The interface Blasto3Q, where 3Q refers to the three qualities of the blastocyst grading, contains a description of 24 variables that were e...
Conference Paper
Full-text available
Abstracts of the 34th Annual Meeting of the European Society of Human Reproduction and Embryology
Article
Follicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus–oocyte complexes derived from these follicles were matured and...
Preprint
Full-text available
We develop an online graphical and intuitive interface connected to a server aiming to facilitate access to professionals worldwide that face problems with bovine blastocysts classification. The interface Blasto3Q (3Q is referred to the three qualities of the blastocyst grading) contains a description of 24 variables that are extracted from the ima...
Preprint
Full-text available
We develop an online graphical and intuitive interface connected to a server aiming to facilitate access to professionals worldwide that face problems with bovine blastocysts classification. The interface Blasto3Q (3Q is referred to the three qualities of the blastocyst grading) contains a description of 24 variables that are extracted from the ima...
Article
Mammalian oocytes resume meiosis spontaneously after removal from the ovarian follicle. We tested the effects of a 2-h prematuration treatment (Pre-IVM) with forskolin (FSK) and 3-isobutyl-1-methylxanthine (IBMX) in bovine cumulus-oocyte complexes (COCs) on the lipid content of oocytes and blastocysts, on the membrane lipid composition of blastocys...
Article
Full-text available
In our study, we added natriuretic peptide type C (NPPC) and/or sildenafil during in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs) followed by in vitro culture (IVC) of embryos with or without sildenafil. We evaluated the effects on the lipid content (LC) of oocytes and embryos and also verified the expression of 96 transcripts r...
Article
Full-text available
Background The timing of the first cell divisions may predict the developmental potential of an embryo, including its ability to establish pregnancy. Besides differences related to metabolism, stress, and survival, embryos with different speeds of development present distinct patterns of gene expression, mainly related to energy and lipid metabolis...
Article
The aim of present work was to assess the effects of pregnancy-associated serum protein A (PAPP-A) during oocyte in vitro maturation (IVM) on meiosis progression, DNA fragmentation, IGF-1 free bioavailability, as well as effects on embryo yield and transcriptional profile of matured cumulus–oocyte complexes (COC). First, the COC from a local abatto...
Poster
During in vitro production (IVP), blastocysts can be differentiated based on the kinetics of early cleavage. These groups present distinct patterns of global DNA methylation, an epigenetic characteristic generally responsible for suppression (presence of methylation) or activation (absence of methylation) of genes from different biological pathways...
Article
Epidermal growth factor receptor (EGFR) activation is an essential step in triggering LH-induced meiotic resumption. Here, we used an EGFR kinase inhibitor (AG1478) to assess the blockage and reversal of meiosis block in bovine oocytes, the competence of such oocytes for the embryonic development, and the gene expression of cumulus cells (CC). Cumu...
Article
The present study aimed to evaluate the effect of the follicular population from cull Nelore (Bos indicus) on the kinetics of in vitro embryonic development. At random stages of the oestrous cycle (Day –5), a total of 28 cull Nelore cows were synchronized with an intravaginal progesterone device associated with oestradiol benzoate (2.0 mg IM). At t...
Article
Full-text available
There is currently no objective, real-time and non-invasive method for evaluating the quality of mammalian embryos. In this study, we processed images of in vitro produced bovine blastocysts to obtain a deeper comprehension of the embryonic morphological aspects that are related to the standard evaluation of blastocysts. Information was extracted f...
Article
Full-text available
Morphological analysis is the standard method of assessing embryo quality; however, its inherent subjectivity tends to generate discrepancies among evaluators. Using genetic algorithms and artificial neural networks (ANNs), we developed a new method for embryo analysis that is more robust and reliable than standard methods. Bovine blastocysts produ...
Article
Early mammalian embryos derived from in vitro fertilization are exposed to conditions distinct from the native oviduct-uterine environment, including atmospheric oxygen that promotes cellular oxidative stress and alters gene expression. High oxygen partial pressure during embryo development is associated with low pregnancy rates and increased embry...
Article
Full-text available
High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O 2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an...