Marcelo Beltrão Molento

Universidade Federal do Paraná | UFPR · Department of Veterinary Medicine

RNA interference has been widely used in parasites. In Haemonchus contortus, reproducible silencing has been reported; however, in this species an altered phenotype has been observed for only a few genes silenced by RNA interference. The aim of this work was to evaluate the effect of silencing Dim-1, a member of the disorganized muscle family on th...

Background: Honey bees and honeycomb bees are very valuable for wild flowering plants and economically important crops due to their role as pollinators. However, these insects confront many disease threats (viruses, parasites, bacteria and fungi) and large pesticide concentrations in the environment. Varroa destructor is the most prevalent disease...

The frequency of gastrointestinal parasites with an emphasis on Strongylus vulgaris was investigated among the Brazilian Pony breed kept on farms in the municipality of Teresópolis, state of Rio de Janeiro. Fecal samples were collected in three stud farms: A (n= 22 animals), B (n= 3), and C (n= 2). Fecal samples were subjected to the quantitative M...

In June 2022, at the XXXII Conference of the Italian Society of Parasitology, the parallels of the main endoparasitic infections of horses and donkeys were discussed. Although these 2 species are genetically different, they can be challenged by a similar range of parasites (i.e. small and large strongyles, and Parascaris spp.). Although equids can...

The aims of this study were to determine the prevalence of helminths in Thoroughbred horses in Rio de Janeiro; make correlations with risk factors for these infections; and compare the efficiency of three floatation solutions applied in the quantitative Mini-FLOTAC technique. Fecal samples from 520 horses were collected from six training centers be...

Thoroughbred horses have been intensely raised for their athletic potential that is correlated with morphological parameters (i.e., body weight - BW, and withers height - WH). Optimum and consistent body development is aimed, but excessive growth rates may lead to the development of orthopedic diseases. This study aimed to generate growth rate curv...

Breast cancer is one of the most common types of cancer in the world and current therapeutic strategies present severe drawbacks. l-carvone (CRV), a monoterpene found in Mentha spicata (spearmint), has been reported to have potent anti-inflammatory activity. Here, we examined the role of CRV in breast cancer cell adhesion, migration and invasion in...

Interest in host epigenetic changes during apicomplexan infections increased in the last decade, mainly due to the emergence of new therapies directed to these alterations. This review aims to carry out a bibliometric analysis of the publications related to host epigenetic changes during apicomplexan infections and to summarize the main studied pat...

Infections caused by Haemonchus spp. and Trichostrongylus spp. are major health problems for sheep and cattle. The objective of this study was to determine the efficacy of copper chloride (CuCl2), and copper sulphate (CuSO4) at 2.0, 7.0, 30.0, 125.0, 500.0, and 2000.0 µM formulations, and nitroxynil 34% (NTX) at 0.235 mM against gastrointestinal ne...

Aims: This study aimed to characterize and compare the antioxidative potential ofextracts obtained by infusion, decoction, tincture, aqueous extraction, and hydroethanolicmaceration from the aerial parts of Polygonum acre H.B.K., which hasbeen traditionally used in herbal preparations, for different purposes. The therapeuticbenefits are attributed...

The genus Polygonum exhibits a variety of biological and therapeutic properties. The goal of this study was to determine the in vitro anthelmintic activity of Polygonum acre extracts against the nematode Haemonchus contortus, characterizing the phenolic profile of these extracts. Aerial parts of P. acre were subjected to hot aqueous and hydroethano...

Strategic helminth control in adult cattle would hardly impose sufficient selection pressure to parasite populations but reports of resistance against macrocyclic lactone (ML) based-products have been confirmed worldwide. The objective of this study was to evaluate the scientific literature of ML resistance (< 90.0% efficiency) in helminths of catt...

The infestation of Rhipicephalus microplus (Acari: Ixodidae) (Canestrini, 1887) represents a significant health problem for cattle. Up until today, no consistent control has been recommended for the free-living larval stage of R. microplus. The objective of the present study was to determine the larvicidal effect of an Allium sativum (garlic) aqueo...

Antecedentes: La tuberculosis es la pandemia que más muertes ha causado a la humanidad. Objetivo. alertar sobre incongruencias que limitan la aproximación al conocimiento real de la tuberculosis y pueden favorecer su expansión durante la COVID-19. Desarrollo: seis incongruencias limitan el conocimiento real de la enfermedad: a) su subestimación, b...

Cyathostomins are considered one of the most important parasites of horses. A group of horses within a herd can be responsible for eliminating the majority of parasite eggs. This phenotype might be explained by genetic factors. This study aimed to identify genomic regions associated with fecal egg count (FEC) and hematological parameters by perform...

Tick control represent a great challenge to animal health. The objective of this study was to evaluate the efficacy of acaricidal compounds against Rhipicephalus microplus from dairy cattle systems in Paraná State, Brazil. Six farms (PR1-PR6) were selected, where anti-tick products were applied at fixed intervals. Two other farms that adopted alter...

Rhipicephalus microplus est l’un des parasites les plus dangereux pour le bétail. De nombreux produits acaricides sont utilisés pour contrôler son infestation. Ceci a engendré une sélection rapide de parasites résistants. Le traitement ciblé sélectif a été proposé à la place du traitement systématique, permettant ainsi à une population sensible de...

The new coronavirus disease 2019 (COVID-19) pandemic has caused a significant impact on mental health worldwide. In South America, COVID-19 has imposed an alarming effect, being the stage of political scandals and the dangerous covid-kit panacea therapy. The objective of the present opinion paper is to give an overview of the impact of COVID-19 on...

Gastrointestinal parasite control has been a major challenge to livestock due to the failure of anthelmintic treatments. Monepantel (MNT) was introduced in 2009 as an alternative treatment option showing a new mechanism of action against nematode parasites. To study the response of MNT in a suppressive regime, 45-Suffolk and White Dorper naturally...

AB S T RA C T Leishmaniasis is a protozoan disease caused by Leishmania spp. and transmitted by sand-flies to different hosts. In Colombia, Leishmania has been reported in dogs from rural and urban areas. The aims of the investigation were to define the risk factors of Leishmania infantum in canine shelters in municipalities of the Bucaramanga metr...

The aim of this study was to determine the prevalence and risk of Fasciola hepatica infection in water buffaloes (Bubalus bubalis) during peripartum in southeastern Mexico. An observational study was designed, in which 94 pregnant buffalo cows near calving and naturally exposed to F. hepatica were included. Farm visits were made on days 60, 30, 15...

Cyathostomins are the most prevalent nematode parasites of horses worldwide and the SLC11a1 gene has been found to modulate the host immune response against parasite infections. The objective of this work was to determine the DNA methylation profile of the SLC11a1 gene in naturally infected horses (n = 6) and correlate it with the cyathostomin feca...

Ivermectin, repositioning, environment, parasites, multi-target drug, COVID-19

O descobrimento, o sucesso comercial e uso social da ivermectina (IVM) sempre foram mundialmente reconhecidos e deu aos seus autores o prêmio Nobel de Medicina/Fisiologia em 2015. Sua exclusiva ação endectocida e segurança, permitiram sua imediata integração em vários protocolos sanitários para praticamente todas as espécies animais, incluindo huma...

O bem-estar de ruminantes, bovinos, ovinos, caprinos e bubalinos, incluindo animais selvagens, depende de um estado de equilíbrio entre nutrição, ambiente/ambiência e a condição sanitária dos animais. Para que estas condições sejam atendidas, temos a responsabilidade de suprir estes animais com a ausência de doença, utilizando os melhores protocolo...

Sheep and goats suffer heavy losses due to the high prevalence of gastrointestinal nematodes, mainly from the nematode Haemonchus contortus. The parasite causes high morbidity and mortality, even though, the indiscriminate use of chemicals to control H. contortus has favored the selection of multidrug resistance parasites. Molecular diagnosis of re...

This study evaluated the in vitro anthelmintic activity of a liquorice ( Glycyrrhiza glabra ) root aqueous extract and of glycyrrhetinic acid at 30, 10, 5, 1, and 0.5 mg/mL against sheep gastrointestinal nematodes (GINs), using the egg hatch test (EHT), the larval development test (LDT), and the larval migration inhibition test (LMIT). The compound...

This study was performed to assess the possible relationship between gastrointestinal strongylid infections and milk quantity and quality in donkeys used for milk production. Individual fecal and milk samples were collected from 16 multiparous lactating jennies of Amiatina breed at their 6th month of lactation. Statistical analysis was carried out...

Sheep farming is of great importance in generating income to smallholders in semi-arid areas worldwide. Gastrointestinal helminths cause important damage to the animals with poor welfare conditions, impacting in local economies. The objective of this work was to evaluate the FAMACHA method (FMC) in a herd of 60 Santa Inês from Paraíba, Brazil, and...

Donkeys, mules, and asses are domestic animals of worldwide distribution, found in all regions of Brazil. Although more health data is needed from these species, some sectors of today’s society are exploring alternatives to use these animals for milk production and the use of donkeys in social events and onotherapy. Donkeys (Equus asinus) are used...

Haemonchus contortus, a blood-sucking parasite of small ruminants, produces very important economic losses in the productive sector. This abomasum parasite has become resistant to most commercial drugs worldwide, and alternatives to fight this problem are urgently needed. Essential oils (EO) are a complex mixture of volatile secondary metabolites,...

The anthelmintic properties and composition of an Italian traditional anthelmintic remedy based on a red algae mixture (RAE) was assessed using the egg hatch test (EHT). The ability of different dilutions (1.0, 5.0, 50, or100%) of RAE was determined and compared with the positive and negative controls against gastrointestinal nematode (GIN) of donk...

Parasitic diseases caused by gastrointestinal nematodes (GIN) are responsible for a major impact on ruminant welfare. Although the available anthelmintics have a safe margin of toxicity to the animals, their indiscriminate use has increased the selection of resistant parasite populations. In this scenario, essential oils (EO) stand out as a promisi...

A ovinocultura é uma considerável fonte de renda para pequenos produtores, como também importante para a produção e exportação de produtos como carne, leite e seus derivados, e lã. Considerando que as helmintoses gastrintestinais em pequenos ruminantes estão listadas como uma das principais causas de diminuição na produção, elas também são responsá...

Os helmintos gastrintestinais de cães são uma das principais fontes de contaminação ambiental e agressão aos animais. O controle dessas parasitoses é importante tanto para a saúde animal como humana. Helmintos dos gêneros Ancylostoma e Toxocara estão envolvidos em casos de zoonoses por exemplo, causando respectivamente a larva migrans cutânea e vis...

Equinos são hospedeiros de uma grande variedade de parasitos. O tratamento supressivo dessas infecções reforçou a ocorrência do fenômeno de resistência parasitária, como pode ser observado neste estudo. Sugere-se a adoção de práticas mais seletivas e estratégicas, visando a preservação do bem estar e sanidade dos animais.

A criação de pequenos ruminantes, possui importância econômica e social no mundo todo. Nesse aspecto, as helmintoses gastrintestinais estão listadas como uma das principais causas de impacto negativo na saúde e bem-estar de ovinos e caprinos. O presente trabalho teve como objetivo determinar a prevalência de helmintos que acometem ovinos. Foram col...

A variety of endo and ectoparasites can affect livestock, causing poor animal performance and low welfare conditions. Haemonchus contortus (Trichostrongyloidae), Rhipicephalus microplus (Ixodidae), Cochliomyia sp. and Lucilia sp. (Calliphoridae) are some of the most important parasites to livestock in Brazil and in many other tropical and subtropic...

RESUMO Essa revisão apresenta considerações acerca da relevância das infecções de parasitos gastrintestinais (PGI) em ruminantes, abordando a importância de fármacos antiparasitários no controle das helmintoses e demais alternativas. Foi realizada uma abordagem conceitual e histórica sobre o controle de PGI e o processo de desenvolvimento da resist...

Background The zoonotic worm parasite Fasciola hepatica secretes an abundance of cathepsin L peptidases that are associated with virulence, invasiveness, feeding and migration. The peptidases are produced as inactive zymogens that activate at low pH by autocatalytic removal of their N-terminal pro-domain or propeptide. Propeptides bind to their cog...

Intestinal strongyles are common helminths of donkeys, in which they may be responsible for disease and poor performance. This study aimed to identify intestinal strongyle genera in 55 naturally infected donkeys from three different farm typologies in Tuscany, central Italy, using morphological and metrical analysis of third stage larvae (L3) obtai...

Nanotechnology has given the tools to human and non-human medicine to improve treatments and to develop effective immune responses against infectious agents. This technology involves the production and use of physical, chemical or biological systems at submicron scales. The funding for nanotechnology research has reached the house of hundreds of mi...

Fasciolosis is a food-borne disease that causes great distress to a range of hosts, including humans. The objectives of this study were to (1) evaluate the liver damage and carcass weight of cattle naturally infected with Fasciola hepatica from the state of Rio Grande do Sul (RS), Brazil, and to (2) determine the distribution of adult flukes in 12,...

A presença do carrapato Rhipicephalus microplus (Canestrini, 1887) em praticamente todo o território nacional e suas graves consequências, permite dizer que este é um dos principais parasitos de bovinos. Muito embora estes animais apresentem algum nível de tolerância ao carrapato, os mesmos podem sofrer com sua presença, quando ocorre um desequilíb...

Fascioliasis is a food-borne parasitic disease that affects a range of animals, including humans caused by Fasciola hepatica. The present study aimed to determine the spatial distribution of bovine fasciolosis and to assess the correlation between the high Positivity Index (PI) and climate data and land altitude, from 2004 to 2008 and 2010 in Santa...

Objetivo. Determinar las pérdidas económicas producidas por el decomiso de hígados afectados y la prevalencia de Fasciola hepatica en bovinos sacrificados en las provincias de Holguín y Camagüey, Cuba. Materiales y métodos. Fueron utilizados los resultados del diagnóstico anatomopatológico realizado en los mataderos Felipe Fuentes y César Escalante...

La fasciolosis es una enfermedad parasitaria que afecta no solo al ganado bovino y ovino sino también al humano, presenta distribución global, con mayor incidencia en zonas tropicales y subtropicales. Las evidencias obtenidas como resultado del proceso investigativo han determinado la inclusión del parásito dentro del grupo de las enfermedades emer...

Fasciola hepatica is a trematode parasite that affects mammals, including humans. In Brazil, fascioliasis, a disease caused by the parasite, is of great importance. The disorder affects the welfare of the Brazilian population through impairing the agricultural production of cattle, where the disease causes weight loss as a result of liver damage. T...

Fascioliasis is a food-borne anthropozoonotic disease caused by Fasciola hepatica that affects multiple hosts, including humans. We herein report the first case of human fascioliasis in the state of Santa Catarina, Brazil. A 57-year-old female patient complaining of abdominal pain was admitted to the hospital for a clinical investigation. The diagn...

In South America, fascioliasis caused by the trematode Fasciola hepatica is an anthropozoonosis disease associated with significant economic losses and poor animal welfare. The objective of this study was to determine the prevalence of F. hepatica in the liver of buffaloes slaughtered from 2003 to 2017 in Brazil, and to perform a forecast analysis...

Morphological biomarkers can be used to establish a diagnosis of fly larvae structural damage and toxicity to target cells by biopesticide candidates. Insecticide activity of natural compounds such as Curcuma longa essential oil (CLLEO) extracted from leaves, and its major constituent α-phellandrene have proven to be a novel biopesticide candidate...

O resultado conjugado desses esforços permitiu traçar o Perfil da Sustentabilidade Industrial do Paraná. Na continuidade, foi investida energia e inteligência na busca de entendimento sobre os fatores que influenciam a conservação e a ampliação da competitividade industrial paranaense, dando origem ao Master Plan de Competitividade 2031. Do desdobr...

Como nos preparamos para o futuro? Como preparamos o futuro? Essas são questões de fundo que vêm, há mais de uma década, norteando a estratégia do Sistema Federação das Indústrias do Estado do Paraná (Sistema Fiep). Provocados por esses questionamentos, temos trabalhado com o firme propósito de melhorar a vida das pessoas a partir de uma atuação co...

The activity of Tagetes minuta essential oil (TMEO) was tested against third instar larvae (L3) of the Australian blowfly Lucilia cuprina. We have determined the potential of the T. minuta EO as a new biopesticide candidate. To test this, groups of 20 L3 were placed on filter paper impregnated with ranging concentrations (from 0.19 to 6.36 μL/cm2)...

The objective of this study was to better understand farmers’ perceptions regarding sheep welfare and procedures concerning the tail docking of sheep in the state of Parana, Southern Brazil. The study was carried out via telephone interviews or personally with 146 sheep farmers. Twenty-eight farmers (19.2%) did not tail dock; the main reasons given...

To determine the existence of seasonal patterns for the economic losses induced by Fasciola hepatica in cattle slaughtered in Camagüey province, Cuba, the monthly records of the 13,059 cattle slaughters were used, carried out in Chacuba slaughterhouse from the Unidad Empresarial Básica Comercializadora ganadera de Terso, belonging to Maraguán Cattl...

The blowfly Lucilia cuprina has great medico-sanitary and veterinary importance due to the ability of its larval form to develop in decaying organic matter, parasitizing vertebrates. Fly eradication is challenging and the essential oil (EO) of Tagetes minuta (TMEO) have been reported to have therapeutic properties. This study aimed to determine the...

The objective of this study was to evaluate the productivity and carcass traits of unweaned Suffolk lambs finished on pasture and subjected to three strategies for endoparasite control: prophylactic treatment of all animals every 28 days; treatment of animals with a cut off number of nematode fecal egg count (FEC) ≥ 700; and treatment of animals us...

El objetivo del estudio fue evaluar la capacidad de protección en ovinos contra Haemonchus contortus de un péptido que mimifica epítopos de gliceraldehído 3-fosfato deshidrogenasa (GAPDH) y Desorganización Muscular (DIM-1) de H. contortus. Se hizo una búsqueda en una biblioteca de fagos (7 aa-GGG) con el empleo de un anticuerpo policlonal (IgG) ant...

Morphological biomarkers as the histopathological assessment and scanning electron microscopy can be used to establish a diagnosis of structure damage and intoxication of target cells by new biopesticide candidate. In this sense, cuticle damage caused by active substances in larvae exposed to biopesticides can help to elucidate the mode action. Thu...

Lucilia cuprina, known as the Australian blowfly, is of high medico-sanitary and veterinary importance due to its ability to induce myiasis. Synthetic products are the most frequent form of fly control, but their indiscriminate use has selected for resistant populations and accounted for high levels of residues in animal products. This study aimed...

Opuntia ficus-indica (L.) Mill. is a xerophylous plant that originated in tropical and subtropical America. This plant is popularly known in Brazil as “palma forrageira” (cactus pear) and plays a fundamental role in animal nutrition, mainly in the Northeastern semi-arid region of the country. The plant has several uses since it presents bioactive c...

Cyathostomins comprise around 50 parasite species of equids, offering a great challenge regarding their individual identification. The objective of our work was to improve identification of infective third stage larvae (L3) with a morphological key supplemented with detailed scientific illustrations based on our research and available literature. T...

The aim of this study was to evaluate phage display technology for mapping Haemonchus contortus mimotopes. We screened the PhD-7 Phage Display Peptide Library Kit with a sheep polyclonal antibody against H. contortus. After four rounds of selection, 50 phage peptide clones were selected by biopanning and sequenced. Two clones displaying peptide mim...