Marcelo Freire

J. Craig Venter Institute | JCVI · Genomic Medicine and Infectious Diseases

Mucosal surfaces such as the gut, vagina and oral cavity are colonized by microbiota that are an integral component of the healthy ecosystem. Recent molecular techniques make it feasible to correlate antimicrobial dosing levels with changes in microbiome composition. The objective of this study was to characterize the rat oral plaque microbiome com...

The objective of this study was to investigate marginal bone level (MBL) changes as the primary outcome of implants placed following healing of extraction sockets treated with ridge preservation, compared to implants placed following unassisted healing. A total of 268 patients (contributing 572 implants) treated from April 2005 to March 2013 were i...

Resolving macrophages counter osteolysis by anabolic actions on bone cells - Supplemental Information

Progression of inflammatory osteolytic diseases, including rheumatoid arthritis and periodontitis, is characterized by increased production of proinflammatory mediators and matrix-degrading enzymes by macrophages and increased osteoclastic activity. Phenotypic changes in macrophages are central to the healing process in virtually all tissues. Using...

The objective of this study was to examine wound healing in extraction sockets following ridge preservation and the outcome of implants placed in those sites. Extraction sockets (N = 31) were grafted with anorganic bovine bone mineral (ABBM) and covered with dense polytetrafluoroethylene membrane. Bone cores obtained during implant placement were e...

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A total of 68 extraction sockets were grafted with anorganic bovine bone mineral and covered by dense polytetrafluoroethylene membrane. Quantitative analysis of three-dimensional microcomputed tomography imaging of core samples retrieved after a mean of 21.0 ± 14.2 weeks revealed 40.1% bone volume fraction (bone volume [BV]/total volume [TV]) and 1...

Non-resolving inflammation is a central pathologic component of obesity, insulin resistance, type 2 diabetes and associated morbidities. The resultant hyperglycemia is deleterious to the normal function of many organs and its control significantly improves survival and quality of life for patients with diabetes. Macrophages play critical roles in b...

Dendritic cell-specific transmembrane protein (DC-STAMP) plays a key role in the induction of osteoclast (OC) cell fusion, as well as DC-mediated immune regulation. While DC-STAMP gene expression is upregulated in the gingival tissue with periodontitis, its pathophysiological roles in periodontitis remain unclear. To evaluate the effects of DC-STAM...

Unresolved inflammation is key in linking metabolic dysregulation and the immune system in type 2 diabetes. Successful regulation of acute inflammation requires biosynthesis of specialized proresolving lipid mediators, such as E-series resolvin (RvE) 1, and activation of cognate G protein-coupled receptors. RvE1 binds to leukotriene B4 (BLT-1) on n...

Staphylococcus aureus–human interactions result in a continuum of outcomes from commensalism to pathogenesis. S. aureus is a clinically important pathogen that asymptomatically colonizes ∼25% of humans as a member of the nostril and skin microbiota, where it resides with other bacteria including commensal Corynebacterium species. Commensal Coryneba...

Periodontal disease exemplifies a chronic and recurrent infection with a necessary biofilm component. Mucosal inflammation is a hallmark response of the host seen in chronic diseases, such as colitis, gingivitis and periodontitis (and the related disorder peri-implantitis). We have taken advantage of our recently developed rat model of human peri-i...

Recently, we have shown that anti-BMP2 monoclonal antibodies (mAbs) can trap endogenous osteogenic BMP ligands, which can in turn mediate osteodifferentiation of progenitor cells. The effectiveness of this strategy requires the availability of the anti-BMP-2 monoclonal antibodies antigen-binding sites for anti-BMP-2 monoclonal antibodies to bind to...

Periodontal diseases are highly prevalent and are linked to several systemic diseases.The goal of periodontal treatment is to halt the progression of the disease and regenerate the damaged tissue. However, achieving complete and functional periodontal regeneration is challenging because the periodontium is a complex apparatus composed of different...

Endogenous molecular and cellularmediators are essential to influence tissue repair andregeneration. We have recently reported the demonstration of a novel strategy for bioengineeringbone, referred to as “antibody mediated osseous regeneration” (AMOR). This entails applicationof specificantibodies capable of capturing endogenous BMP-2 molecules in...

Supplementary Figure S1. Protein homology between human and rabbit BMP-2. The primary amino acid sequences of human and rabbit BMP-2 were aligned and calculated the similarity of them using the CLUSTAL W algorithm in the MegAlign program (DNAStar LasergeneTM 8.0, DNAStar Inc., Madison, WI, USA). Pairwise comparison is represented in similarity perc...

Diabetic complications involve inflammation-mediated microvascular and macrovascular damage, disruption of lipid metabolism, glycosylation of proteins, and abnormalities of neutrophil-mediated events. Resolution of inflamed tissues to health and homeostasis is an active process mediated by endogenous lipid agonists, including lipoxins and resolvins...

Therapies to reverse tissue damage from osteolytic inflammatory diseases are limited by the inability of current tissue-engineering procedures to restore lost hard and soft tissues. There is a critical need for new therapeutics in regeneration. In addition to scaffolds, cells, and soluble mediators necessary for tissue engineering, control of endog...

Objective: Bioengineering strategies focus on delivering scaffold materials and growth factors to modulate cellular and tissue responses. However, early innate immune events, such as coagulation and local inflammation, that are crucial for healing have not been the target of therapeutic strategies. The goal of this investigation was to evaluate the...

Biomaterials are widely used as scaffolds for tissue engineering. We have developed a strategy for bone tissue engineering that entails application of immobilized anti-BMP-2 monoclonal antibodies (mAbs) to capture endogenous BMPs in vivo and promote antibody-mediated osseous regeneration (AMOR). The purpose of the current study was to compare the e...

Biomaterials are widely used as scaffolds for tissue engineering. We have developed a strategy for bone tissue engineering that entails application of immobilized anti-BMP-2 monoclonal antibodies (mAbs) to capture endogenous BMPs in vivo and promote antibody-mediated osseous regeneration (AMOR). The purpose of the current study was to compare the e...

http://www.quintpub.com/display_detail.php3?psku=B6508#.Uvnoe0JdX6Q In today's health care environment, the emphasis on disease prevention means that, more than ever before, dentists are expected to promote the general as well as oral health of their patients. Over the past several decades, an impressive body of knowledge has accumulated about the...

Inflammation is a protective response essential for maintaining human health and for fighting disease. As an active innate immune reaction to challenge, inflammation gives rise to clinical cardinal signs: rubor, calor, dolor, tumor and functio laesa. Termination of acute inflammation was previously recognized as a passive process; a natural decay o...

The posterior edentulous maxilla is a critical anatomic region for dental implant therapy. Because of severe alveolar bone resorption and maxillary sinus pneumatization, low bone volume is often presented clinically. Although maxillary sinus augmentation has been developed to promote bone reconstruction and oral rehabilitation, complications have b...

Inflammation is a protective response essential for maintaining human health and for fighting disease. As an active innate immune reaction to challenge, inflammation gives rise to clinical cardinal signs: rubor, calor, dolor, tumor and functio laesa. Termination of acute inflammation was previously recognized as a passive process; a natural decay o...

Mucosal surfaces such as the gut, vagina and oral cavity are colonized by microbiota that are an integral component of the healthy ecosystem. Recent molecular techniques make it feasible to correlate antimicrobial dosing levels with changes in microbiome composition. The objective of this study was to characterize the rat oral plaque microbiome com...

Objective: Loss of inflammation regulation is a biological event common to human chronic inflammatory diseases including Type II diabetes mellitus and periodontitis. Resolution of inflammation requires cellular activation by resolution agonists, such as RvE1, through its G protein-coupled receptor, ChemR23. The expression of chemR23 on various cell...

Aim: To evaluate the efficacy of a nonthermal plasma (NTP) at atmospheric pressure on ex vivo biofilm in root canals of extracted teeth. Methodology: Intracanal contents from three teeth with root canal infections were collected, pooled and grown in thirty-five microCT-mapped root canals of extracted and instrumented human teeth. One group of te...

Bone engineering strategies often exploit modulation of the extracellular environment, including delivery of exogenous growth factors to regenerate damaged tissues. We have recently reported a different approach, referred to as "antibody mediated osseous regeneration" (AMOR), which exploits the specificity of monoclonal antibodies (mAbs) to capture...

The antimicrobial activity of ursolic acid (UA) and oleanolic acid (OA), both triterpenoid compounds, against methicillin-resistant Staphylococcus aureus (MRSA) is controversial. We examined the antimicrobial effects of UA and OA against 19 strains of MRSA isolated from Koreans by determining minimum inhibitory concentrations (MIC) and minimum bact...

Nanosecond pulsed atmospheric-pressure plasma jet has been applied for bacterial biofilm disinfection as a potential supplement or alternative disinfection tool for root canal treatment. [1,2,3] We present an ex vivo study of the antimicrobial effect of the cold plasma jet on an endodontic biofilm model. Endodontic biofilms were grown in microCT-ma...

Sponsored by: Craniofacial Biology Description: Tissue Regeneration studies sit at the crossroads of cell biology and clinical medicine. Because of this unique position, uncovering the specific role of stem cells, their niches and signaling molecules that modulate the microenvironment is one of the most lively and diverse fields in basic biomedica...

Bone engineering strategies often exploit modulation of the extracellular environment, including delivery of cell and growth factors to repair and regenerate damaged tissues. During bone healing, the expression of endogenous bone morphogenetic proteins is an essential component of the healing response. However, in some situations, the inherent repa...

Linalool and α-terpineol exhibited strong antimicrobial activity against periodontopathic and cariogenic bacteria. However, their concentration should be kept below 0.4 mg/ml if they are to be used as components of toothpaste or gargling solution. Moreover, other compounds with antimicrobial activity against periodontopathic and cariogenic bacteria...

Objective :The purpose of this study was to characterize the biological and molecular mechanisms of tethered monoclonal antibodies (mAbs) in the extracellular microenvironment. Methods : In vivo analyses was performed by immobilizing Anti-BMP-2 mAb , isotype control Ab (25 ug/ml), were adsorbed in each of the scaffolds by incubation at room tempera...

Objectives: The purpose of the current study was to examine the efficacy of immobilized anti-BMP-2 antibodies on 4 different biomaterials in promoting Antibody Mediated Osseous Regeneration (AMOR). Methods: Four scaffold used included, Ti microbeads, alginate hydrogel (Alg), beta-TCP and absorbable collagen sponge (ACS). Anti-BMP-2 mAb (25 ug/ml),...

Research is vital to growth and knowledge. In a profession such as dentistry advancement made possible through research translates rather simply into better patient care. While the importance of individual research efforts in dentistry will always be significant, collaborative efforts have the potential to bring goals of individuals to new...

Bone regeneration often requires harvesting of autologous bone with significant potential morbidity and cost. Recombinant human bone morphogenetic protein (rhBMP)-2 has been approved by the U.S. Food and Drug Administration for specific regenerative indications. However, administration of exogenous growth factors has many drawbacks. The objective o...

Biofilm-induced inflammatory osteolytic oral infections, such as periodontitis and peri-implantitis, have complex etiology and pathogenesis. A significant obstacle to research has been the lack of appropriate animal models where the inflammatory response to biofilms can be investigated. The aim of this study is to develop a novel animal model to st...

Objectives:Biofilm-induced inflammatory osteolytic oral infections such as periodontitis and peri-implantitis have complex etiology and pathogenesis. The lack of appropriate animal models, where the inflammatory response to pathogenic biofilms can be investigated has been obstacle to research. The aim of this study was to develop a novel animal mod...

Objectives:Tissue engineering strategies often uses manipulation of the extracellular environment to change cell fate and guide tissue formation. Immunomodulation effects of the extracellular matrix by monoclonal antibodies to pormote bone regeneration are not known. The purpose of the study was to determine whether immobilized anti-BMP-2 antibodie...

Regeneration of bone in the craniofacial area is necessary for treatment of congenital anomalies, traumatic injuries, pathological osteolytic conditions, as well as for reconstruction of atrophic jaws. Approaches for bone regeneration often require harvesting of autologous bone from the iliac crest, tibia, ribs or craniofacial bones. Development o...

Bone Morphogenetic Protein 2 (BMP-2) plays an essential role in osteoblast differentiation, skeletal development, as well as in early embryonic development. Recombinant human BMP-2 (rhBMP-2) has been approved by FDA for a number of clinical applications, including reconstruction of maxillofacial defects. Objectives: The present study sought to deve...