Marcello Mele

Università di Pisa | UNIPI · Department of Agriculture, Food and Environment (DAFE)

We compared the effect of two different kinds of steam oven—a standard (SO) and a superheated steam (SHS) oven—on four different kinds of samples: hamburgers, bovine steaks, pork steaks, and salmon fillets. Ten samples of each meat/fish were divided into three parts. Then samples were analyzed as (i) raw, (ii) cooked with SO, and (iii) cooked with...

Although increasingly used in animal feeding, the black soldier fly (Hermetia illucens - HI) and mealworm (Tenebrio molitor - TM) larvae and pupae require the characterization and modulation of their fat profile for use in the feed industry. In this study, we provide a comprehensive fatty acid profile of the total lipid triglycerides and phospholip...

Robust Animal-Based Measures (ABMs) are fundamental in order to assess animal welfare, however in semi-extensive sheep farming systems is not easy to collect ABMs without inducing additional stress in the animals. Heart rate variability (HRV) is a non-invasive technique of assessing stress levels related to animal welfare. It is considered a sensit...

Tannins and essential oils (EOs) have been previously described for their properties to mitigate ruminal methane and ammonia. Their combination might be even more efficient, as they have different modes of action on rumen pathways. This study aimed to screen in vitro the mitigating properties of variously combining tannins with EO in a total of 48...

Tuscany belongs to the Mediterranean area which has unique characteristics related to its climate and cultural conditions; in this geographical area, sheep are primarily bred in semi-extensive or extensive systems. The present study aimed to survey the raw sheep milk produced in sheep farms of Tuscany and to explore the role of different management...

This study aimed to assess consumer knowledge, attitudes, and perceptions toward dairy products from sheep and goats. A web-based survey was conducted in Latin America (Mexico and Chile), Europe (Italy, Spain, Greece, and Denmark), and Asia (Bangladesh). From March to June 2021, adult participants answered an online survey available in 5 languages....

The aims of the present study were to evaluate the preventive and the therapeutic effect of Stodi ® as phytogenic feed additive rich in phenolic substances on the calf diarrhea, during the first 24 days of life. A total of 40 calves were included and randomly divided into Group C (control group) and Group T (treated group) with placebo or treatment...

Dairy cows have high incidences of metabolic disturbances, which often lead to disease, having a subsequent significant impact on productivity and reproductive performance. As the milk fatty acid (FA) profile represents a fingerprint of the cow’s nutritional and metabolic status, it could be a suitable indicator of metabolic status at the cow level...

The rumen is characterised by a complex microbial ecosystem, which is particularly active in lipid metabolism. Several studies demonstrated a role of diet and breed on bacterial community profile, with the effect on metabolic pathways. Despite the knowledge achieved on metabolism and the bacterial profile, little is known about the relationship bet...

A multivariate analysis was used to investigate the fatty acid (FA) profile in three different Italian sheep breeds: Comisana, Massese, and Sarda. A sample of 852 animals was considered: 118 Massese, 303 Comisana, 431 Sarda. Sarda sheep were divided into two groups, based on their breeding origin (298 and 133 reared in Sardinia and Tuscany, respect...

Pink discoloration defect can cause economic losses for cheese producers due to the impossibility to sell the defected cheese, but few knowledge is currently available on the causes of this defect. To gain more insight on the causes that lead to the formation of pink discoloration in Pecorino Toscano cheese with the Protected Designation of Origin...

Epidemiological studies have demonstrated a positive relationship between dietary fat intake and the onset of several metabolic diseases. This association is particularly evident in a diet rich in saturated fatty acids, typical of animal foods, such as dairy products. However, these foods are the main source of fatty acids with a proven nutraceutic...

Dairy sheep systems are relevant for the economy of many rural areas of the Mediterranean Basin and the optimisation of their productive factors is necessary to improve their competitiveness and to reduce their environmental impact. The objective of this study was to assess, through a farm-scale life cycle assessment (LCA) approach, the potential o...

The global consumption, notably in developing countries, and production of beef are increasing continuously, and this requires the industry to improve performance and to reduce the environmental impact of the production chain. Since the improvement in efficiency and the highest impacts occur at farm level, it is appropriate to focus on the profitab...

Several dietary strategies were adopted to reduce saturated fatty acids and increase beneficial fatty acids (FA) for human health. Few studies are available about the pathways/genes involved in these processes. Illumina RNA-sequencing was used to investigate changes in the ovine mammary gland transcriptome following supplemental feeding with 20% ex...

Traditional fermented foods are obtained by a complex consortium of autochthonous microorganisms producing a wide variety of bioactive compounds, thus representing a reservoir of strains with new functional properties. Here, doughs obtained using five different wholegrain flours were singly fermented with selected yeast strains, which were evaluate...

The livestock sector constitutes 14.5% of global green-house gas (GHG) emissions and soil and water pollution due to nitrogen excretion. Methane and nitrogen excretions in ruminants can be mitigated by specific feeding strategies, and tannins reduce methanogenesis and ammonia syntheses. In our study, two kinds of condensed tannins (Mimosa and Gambi...

Body measurements could be used to estimate body weight (BW) with no need for a scale. The aim was to estimate heifers weight based on their body dimension characteristics. Twenty-five Holstein heifers represent the study group (SG); another 13 animals were evaluated as a validation group (VG). All the heifers were weighed (BW) and their wither hei...

In the past 10 years, the average demand for meat and milk across the world has significantly increased, especially in developing countries. Therefore, to support the production of animal-derived food products, a huge quantity of feed resources is needed. This paper does not present original research, but rather provides a conceptual strategy to im...

We evaluated the preventive effects of the oral administration of chestnut tannins (Castanea sativa) together with its potential metabolic effect on calf diarrhoea. Forty Italian Friesian female calves were included and divided into Group C (control group) and Group T (tannin-treated group). From the third day of life (T0) for the following 56 days...

The use of rustic cattle is desirable to face challenges brought on by climate change. Maremmana (MA) and Aubrac (AU) are rustic cattle breeds that can be successfully used for sustainable production. In this study, correlations between two rearing systems (feedlot and grazing) and the rumen microbiota, the lipid composition of rumen liquor (RL), a...

A meta-analysis was conducted to analyze the effects of different dietary vegetable sources rich in unsaturated FA (UFA) on sheep cheese FA profile. This study also quantified the overall effect of feeding sheep with vegetable sources rich in UFA (linseed, flaxseed, sunflower seed, canola, olive oil, bran oil, and olive cake), on milk yield (MY) an...

This study aimed to characterise the fibre composition of Triceps brachii (TB) and Semimembranosus (SM) muscles from 20 Maremmana (MA) and 20 Aubrac (AU) steers, and the effect of grazing activity in comparison with feedlot system. The histochemical method was performed with the m-ATPase method with an acid pre-incubation, thus allowing to distingu...

Cropping among trees with perennial legumes is one option for increasing agro-ecosystem services, such as improving the nitrogen supply and increasing soil protection by herbaceous vegetation. Moreover, cropping under the canopy of olive trees should diversify the farm production, compared to the traditional fallow management. Among perennial legum...

Genomic selection (GS) reports on milk fatty acid (FA) profiles have been published quite recently and are still few despite this trait represents the most important aspect of milk nutritional and sensory quality. Reasons for this can be found in the high costs of phenotype recording but also in issues related to its nature of complex trait constit...

This study determined production performance, nutrient digestibility, and milk composition of dairy ewes supplemented with crushed sunflower seeds (Helianthus annuus) and sunflower seed silage in corn silage-based diets. Six ewes were grouped in a double 3 × 3 Latin square design with three periods of 21 days. All treatments were based on ad libitu...

The omega-3 fatty acid (FA) enrichment of yolk is a key means one of the main objec- tives to improve the nutraceutical properties of eggs. We evaluated the effect of the dietary inclusion of extruded linseed fed to laying hens on the fatty acid composition of the polar and non-polar lipid classes of the eggs. Two groups of 36 Lohmann White Leghorn...

In the Mediterranean area, semi-extensive mixed farming systems characterise the inland landscape and guarantee the maintenance of several agroecosystem services. Rationed and rotational grazing have been proposed as efficient agroecological practices for sustainable intensification. Previous studies have highlighted that, during spring, restricted...

Poplar (Populus L. spp.) Short Rotation Coppice systems (SRCs) for bioenergy production are being converted back to arable land. Transitioning to Alley Cropping Systems (ACSs) could be a suitable strategy for integrating former tree rows and arable crops. A field trial (Pisa, Central Italy) was set up with the aim of assessing the C storage of an A...

Immunoglobulin type G (IgG) concentration both in jennies' colostrum and in serum of donkey foals are mostly unknown in the first 24 h after delivery. The aims of the present study were to evaluate the IgG concentrations of colostrum during the first 24 h of lactation of Amiata jennies, the absorption of colostrum and the weekly body weight gain of...

Milk fatty acid (FA) profile is a clear example of complex and multiple correlated traits whose genetic basis is difficult to assess. Although genome-wide association (GWA) studies have been successful in the identification of significant genetic variants for complex traits, when correlated phenotypes are analysed separately, the outcomes are diffi...

The present study investigated whether the fatty acid composition of milk changes in relation to an increase in the milk somatic cell count (SCC) of separate udder quarters. We investigated the potential of multivariate factor analysis to extract metabolic evidence from data on the quantity and quality of milk of quarters characterized by different...

Donkey placenta does not allow the passage of immunoglobulins, thus foals are born hypo-gammaglobulinemic and an adequate intake of high-quality colostrum in the first 24 hours of life is crucial for the surviving. The study aims to assess the relation between colostrum IgG concentration evaluated by the single radial immunodiffusion (SRID) test an...

Kiwifruit is an interesting alternative to chymosin for milk coagulation. Although the clotting properties of actinidin (the proteolytic agent present in kiwi) have been widely investigated, little is known about the nutraceutical and organoleptic effects of kiwifruit on the characteristics of cheese. We investigated kiwifruit pulp, compared to cal...

Calves are born almost agammaglobulinemic and thus need an adequate amount of good quality colostrum to avoid failure of transfer of passive immunity. The quality of colostrum is influenced by several factors such as the volume of colostrum produced, collection time, the concentration of immunoglobulins, breed, the age of dam, and mastitis events i...

Agroforestry is indicated as a farming practice suited to enhance ecosystem services generated by cropping systems. However, farmers are often reluctant to implement agroforestry systems due to the potential yield loss of crops. In a field trial, soybean was intercropped with poplar short-rotation-coppice rows in an alley-cropping system with 13.5...

Aging is characterized by dynamic changes at metabolic level that lead to modifications in the composition of the metabolome. Since the identification of biomarkers that can discriminate people of different age and health status has recently attracted a great interest, we wondered whether age-specific changes in the metabolome could be identified a...

The successful promotion of agroforestry in Italy depends on both a recognition of tradition and the opportunities for innovation. In Italy, agroforestry has traditionally been a key component of landscape management. Complex systems, based on the integration among crops–livestock–fruit/forest trees, provided a wide variety of products (e.g. food,...

Ruminant fats are characterized by different levels of conjugated linoleic acid (CLA) and α-linolenic acid (18:3n-3, ALA), according to animal diet. Tissue fatty acids and their N-acylethanolamides were analyzed in male obese Zucker rats fed diets containing lamb meat fat with different fatty acid profiles: (A) enriched in CLA; (B) enriched in ALA...

Feeding is an important factor that affects the quality of animal products and also has a major impact on regional and local dairy products in terms of origin and quality. This study investigated the effects of pasture on nutritional characteristics and volatile components of sheep's milk, yoghurt and cheese produced in central Italy. During two gr...

The addition of polyphenol extracts in ruminant diets is an effective strategy to modulate rumen microflora. The aim of this in vitro trial was to study the effects of chestnut tannin extract (CHT), vescalagin (VES) and gallic acid (GAL) on dietary fibre degradability and on the dimethyl acetals (DMA) profile and microbial community composition of...

Flaxseed is well known for its nutritional/nutraceutical value and it is commonly cultivated for oil production. This process generates considerable amounts of by-products, such as oil cake. This study aimed at quantifying the nutrients and nutraceuticals content of cake and at checking their maintenance during storage in paper or polypropylene bag...

The objective of the study was to test the associations between backfat fatty acid composition (FAC)in a sample of 536 Italian Large White pigs and single nucleotide polymorphisms (SNPs)located in candidate genes, using univariate and multivariate approaches. The strongest associations were identified for the SNP AY183428 c.265T>C in Fatty acid syn...

Agroforestry and ecological intensification. The agricultural and forestry sectors are currently facing the dramatic tri-lemma of producing food, energy and wood for a growing world population, while preserving the environment at the same time. Agroforestry systems are able to address those issues by integrating crops and/or grazing animals with wo...

The aim of this study was to perform genetic, genome-wide association (GWAS), and gene-set enrichment analyses with latent variables related to milk fatty acid profile (i.e., fatty acids factor scores; FAF), milk composition , and udder health in a cohort of 1,158 Italian Brown Swiss cows. The phenotypes under study were 12 FAF previously identifie...

The interest of the scientific community in the effects of plant polyphenols on animal nutrition is greatly increasing. These compounds, in fact, are ubiquitous in the plant kingdom, especially in some spontaneous plants exploited as feeding resources alternative to cultivated crops and in several agro-industry by-products. Polyphenols interact wit...

Figure S1. Representative immunoblot showing the specificity of Plin2 downregulation by in vivo RNAi technique in adult Tibialis anterior (TA) muscle fibers. TA muscles were transfected with vectors expressing two different short harpin RNAs (shRNAs) specific for Plin2. The expression of Plin3 and Plin5 (two Plins present in skeletal muscle fibers)...

Figure S2. A‐B: Cross Sectional Area (CSA) of TA muscles upon knockdown of Plin2 expression. A: CSA of adult TA muscle fibers transfected with either shPlin2 or scramble. Left columns: *p = 0.001; right columns: the same, plus treatment with rapamycin: *p = 0.02. B: CSA of adult TA muscle fibers transfected with shPlin2 or scramble and undergone de...

Figure S3. A: Representative images of succinate dehydrogenase (SDH) from Tibials anterior (TA) transversal sections isolated from scramble and shPlin2 mice analyzed by SDH staining. B: Fluorescence microscope images (in 5x magnification) of a whole TA section of mouse transfected with shPlin2, and the SDH respectively staining.

Figure S4. A: Western blot analysis showing the reduction in S6 phosphorylation, demonstrating the corrected mTORC1 inhibition by rapamycin treatment; CMC: carboxymethylcellulose. B: Schematic representation of Plin2 downregulation and denervation experiments performed in the same mouse.

Climate change alters the thermal environment of animals, affecting animal health, reproduction, and the feed conversion efficiency. Environmental stress and, more specifically, thermal stress directly affect productivity and health of livestock resulting in significant economic losses. Agroforestry is a land-use strategy to cope with climate chang...

Twenty young Maremmana bulls were randomly allotted to two dietary groups during a finishing period (~90 days): C diet (without lipid supplementation), and L diet (with linseed supplementation, 20% concentrate feed). The aim was to investigate the effects of dietary linseed supplementation on the intramuscular fat composition, and the shelf-life of...

Objectives of this study were to evaluate effects of grazing tropical forage species and level of supplementation with grain on characteristics of lamb meat. Ninety-day-old lambs (n = 36) (22.54 ± 2.72 kg) were randomly assigned to a 2 x 3 factorial arrangement of pasture types (Aruana and Marandu) and levels of concentrate supplementation (0%, 1.5...

Background Perilipin2 (Plin2) belongs to a family of five highly conserved proteins, known for their role in lipid storage. Recent data indicate that Plin2 has an important function in cell metabolism and is involved in several human pathologies, including liver steatosis and Type II diabetes. An association between Plin2 and lower muscle mass and...

Bovine milk is important for human nutrition, but its fat content is often criticized as a risk factor in cardiovascular disease. Selective breeding programs could be used to alter the fatty acid (FA) composition of bovine milk to improve the healthiness of dairy products for human consumption. Here, we performed a genome-wide association study (GW...

Subcutaneous fat thickness and fatty acid composition (FAC) play an important role on seasoning loss and organoleptic characteristics of seasoned hams. Dry-cured ham industry prefers meats with low contents of polyunsaturated fatty acids (PUFA) because these negatively affect fat firmness and ham quality, whereas consumers require higher contents i...

Background: Neonatal calf diarrhea is generally caused by infectious agents and is a very common disease in bovine practice, leading to substantial economic losses. Tannins are known for their astringent and anti-inflammatory properties in the gastro-enteric tract. The aim of this study was to evaluate the effect of the oral administration of ches...