Marcel Polling

Marcel Polling
Wageningen University & Research | WUR · Wageningen Enviromental Research

Combining AI and DNA metabarcoding for quantification and smart sampling strategies

About

19
Publications
4,145
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236
Citations
Citations since 2016
11 Research Items
217 Citations
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060

Publications

Publications (19)
Article
Pollen classification is considered an important task in palynology. In the Netherlands, two genera of the Urticaceae family, named Parietaria and Urtica, have high morphological similarities but induce allergy at a very different level. Therefore, distinction between these two genera is very important. Within this group, the pollen of Urtica membr...
Article
Full-text available
Biological particles suspended in the atmosphere have a crucial role in the dynamics of the biosphere underneath. Although much attention is paid for the chemical and physical properties of these particles, their biological taxonomic identity, which is relevant for ecological research, remains little studied. We took air samples at 300 meters above...
Article
Full-text available
The study of faecal samples to reconstruct the diets and habitats of extinct megafauna has traditionally relied on pollen and macrofossil analysis. DNA metabarcoding has emerged as a valuable tool to complement and refine these proxies. While published studies have compared the results of these three proxies for sediments, this comparison is curren...
Article
Full-text available
Airborne pollen monitoring is of global socio-economic importance as it provides information on presence and prevalence of allergenic pollen in ambient air. Traditionally, this task has been performed by microscopic investigation, but novel techniques are being developed to automate this process. Among these, DNA metabarcoding has the highest poten...
Article
Full-text available
Monitoring of airborne pollen concentrations provides an important source of information for the globally increasing number of hay fever patients. Airborne pollen is traditionally counted under the microscope, but with the latest developments in image recognition methods, automating this process has become feasible. A challenge that persists, howev...
Preprint
Full-text available
Monitoring of airborne pollen concentrations provides an important source of information for the globally increasing number of hay fever patients. Airborne pollen are traditionally counted under the microscope, but with the latest developments in image recognition methods, automating this process has become feasible. A challenge that persists, howe...
Article
Full-text available
Several studies indicate that North Atlantic Deep Water (NADW) formation might have initiated during the globally warm Eocene (56–34 million years ago; Ma). However, constraints on Eocene surface ocean conditions in source regions presently conducive to deep‐water formation are sparse. Here we test whether ocean conditions of the middle Eocene Labr...
Poster
Full-text available
What combination of DNA metabarcoding primers works best to reconstruct changing species composition of Arctic vegetation over time? How do pollen, macro-fossil and DNA results from Pleistocene coprolites compare?
Conference Paper
Full-text available
Recent data shows increasing numbers of hay fever patients, with approximately 10-30% of the population affected worldwide (Pawankar et al. 2011). This increase is most likely caused by prolonged and intensified pollen seasons which in turn have been linked to increased CO 2 concentrations (Ziska et al. 2003, D'Amato et al. 2007, Albertine et al. 2...
Conference Paper
Hydrocarbon exploration in the deep-water GoM (Gulf of Mexico) has fuelled increased investment into the potential for palynology to answer fundamental stratigraphic and depositional problems offshore especially since the calcareous nanno- and microfossils are sparse in these plays. Pollen and spore floras are abundant and rich in the Midway, Wilco...
Article
Full-text available
Recent studies have shown that the Early Eocene Climatic Optimum (EECO) was preceded by a series of short-lived global warming events, known as hyperthermals. Here we present high-resolution benthic stable carbon and oxygen isotope records from ODP Sites 1262 and 1263 (Walvis Ridge, SE Atlantic) between ~ 54 and ~ 52 million years ago, tightly cons...
Data
Recent studies have shown that the Early Eocene Climatic Optimum (EECO) was preceded by a series of short-lived global warming events, known as hyperthermals. Here we present high-resolution benthic stable carbon and oxygen isotope records from ODP Sites 1262 and 1263 (Walvis Ridge, SE Atlantic) between ~54 and ~52 million years ago, tightly constr...
Article
Full-text available
The Paleocene–Eocene Thermal Maximum (PETM) represents a ~170 kyr episode of anomalous global warmth ~56 Ma ago. The PETM is associated with rapid and massive injections of 13C-depleted carbon into the ocean–atmosphere system reflected as a prominent negative carbon isotope excursion (CIE) in sedimentary components. Earth's surface and deep ocean w...
Conference Paper
The Late Paleocene- Early Eocene warming trend (55-50 Ma) is marked by a series of short-lived global warming events, known as hyperthermals. The Paleocene-Eocene Thermal Maximum, (PETM), is the most pronounced, followed by the ETM2 (Elmo) and the ETM3 ("X-event") up to the Early Eocene Climatic Optimum (EECO). These transient greenhouse episodes a...
Article
Full-text available
The Paleocene-Eocene thermal maximum (PETM) represents a ~170 kyr episode of anomalous global warmth ~56 Ma ago. The PETM is associated with rapid and massive injections of 13C-depleted carbon into the ocean-atmosphere system reflected as a prominent negative carbon isotope excursion (CIE) in sedimentary components. Earth's surface and deep ocean w...
Poster
Full-text available
The Paleocene-Eocene thermal maximum (PETM) represents a ~170kyr episode of anomalous global warmth ~55.8 Myr ago, with the onset being marked by a geologically abrupt negative carbon isotope excursion (CIE) in both marine and continental sedimentary components. Reflecting the massive release of 13C-depleted carbon into the ocean and atmospheric sy...
Conference Paper
Full-text available
Increasingly high resolution isotope- and novel organic geochemical proxy records have revealed that the long-term cooling trend of the middle Eocene was interrupted by a warming phase designated the Middle Eocene Climatic Optimum (MECO). It is suggested to represent an increase in sea surface temperatures of about 4°C, lasting approximately 400 k...

Questions

Question (1)
Question
Hello all,
My question relates to pollen that have been collected by a Burkhard type pollensampler. I have access to a historic collection of daily samples dating back to 1969 (we have all pollen counts). We are currently investigating the possibilities to utilize this amazing record and find new trends that cannot be studie with Light Microscopy alone.
One idea is to try and perform metabarcoding/metagenomics on the samples. One possible issue is that the pollen have been stained in safranin (mounted in glycerin, gelatin and phenol). Does anyone know whether it would still be possible to extract DNA from these pollen?
Very curious to hear your thoughts,
Thanks a lot,
Marcel

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