Marc Stadler

Marc Stadler
Helmholtz Centre for Infection Research | HZI · Department Microbial Drugs (MWIS)

Ph. D.
President-elect, International Mycological Association (IMA) Co-ordinator, EU H2020-MSCA-RISE project Mycobiomics

About

521
Publications
310,776
Reads
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16,595
Citations
Citations since 2017
280 Research Items
12366 Citations
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201720182019202020212022202305001,0001,5002,0002,5003,000
201720182019202020212022202305001,0001,5002,0002,5003,000
201720182019202020212022202305001,0001,5002,0002,5003,000
Introduction
Our mission is to exploit the global fungal and microbial biodiversity in order to discover novel useful secondary metabolites like antibiotics and anticancer agents and make these metabolites available for evaluation in preclinical development. We are combining skills in taxonomy, phylogeny, microbiology, mycology, analytical chemistry, biotechnology and pharmacology in an international interdisciplinary scenario involving young scientists from all over the world. .
Additional affiliations
January 2014 - present
Helmholtz Centre for Infection Research
Position
  • Biotechnologiy/Fermentation course
Description
  • Course for BSc Biotechnology Topics Fermentation, Downstream processing and isolation of biologically active secondary metabolites from fungi, actinobacterial and myxobacteria
January 2013 - present
Deutsches Zentrum für Infektionsforschung DZIF
Deutsches Zentrum für Infektionsforschung DZIF
Position
  • Task leader
August 2012 - present
Chinese Academy of Sciences
Position
  • Professor
Education
June 2009 - January 2012
University of Bayreuth
Field of study
  • Mycology
January 1991 - June 1993

Publications

Publications (521)
Article
Full-text available
A multigene phylogeny was constructed including a significant number of representative species of the main lineages in the Xylariaceae and four DNA loci – the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA; the second largest subunit of the RNA polymerase II (RPB2) and beta-tubulin (TUB2). Specimens were selec...
Article
The diversity of secondary metabolites in the fungal order Xylariales is reviewed with special emphasis on correlations between chemical diversity and biodiversity as inferred from recent taxonomic and phylogenetic studies. The Xylariales are arguably among the predominant fungal endophytes, which are the producer organisms of pharmaceutical lead c...
Article
The Basidiomycota constitutes the second largest higher taxonomic group of the Fungi after the Ascomycota and comprises over 30.000 species. Mycelial cultures of Basidiomycota have already been studied since the 1950s for production of antibiotics and other beneficial secondary metabolites. Despite the fact that unique and selective compounds like...
Article
Full-text available
The Hypoxylaceae (Xylariales, Ascomycota) is a diverse family of mainly saprotrophic fungi, which commonly occur in angiosperm-dominated forests around the world. Despite their importance in forest and plant ecology as well as a prolific source of secondary metabolites and enzymes, genome sequences of related taxa are scarce and usually derived fro...
Article
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Fungi are an understudied resource possessing huge potential for developing products that can greatly improve human well-being. In the current paper, we highlight some important discoveries and developments in applied mycology and interdisciplinary Life Science research. These examples concern recently introduced drugs for the treatment of infectio...
Article
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Chemical investigation of an endophytic fungus herein identified as Diaporthe cf. ueckeri yielded four known compounds, named cytochalasins H and J and dicerandrols A and B. Reports of acid sensitivity within the cytochalasan family, inspired an attempt of acid-mediated conversion of cytochalasins H and J resulting in the acquisition of five polycy...
Presentation
Full-text available
The Hypoxylaceae are one of the largest families of Xylariales (Ascomycota) and have been a main subject of our polyphasic taxonomic studies and bioprospecting approaches over the past decade. Several hundreds of strains have been isolated from ascomata collected in the field, subjected to fermentation, and the cultures were extracted with organic...
Article
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Methicillin-resistant Staphylococcus aureus (MRSA) is a World Health Organization's high priority pathogen organism, with an estimated > 100,000 deaths worldwide in 2019. Thus, there is an unmet medical need for novel and resistance-breaking anti-infectives. The natural product Corallopyronin A (CorA), currently in preclinical development for filar...
Article
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Protein microarray screenings identified fungal natural products from the azaphilone family as potent inhibitors of SARS-CoV-2 spike protein binding to host ACE2 receptors. Cohaerin F, as the most potent substance from the cohaerin group, led to more than 50% less binding of ACE2 and SARS-CoV-2 spike protein. A survey for structurally related azaph...
Poster
Full-text available
The MYCOBIOMICS project is funded by the Horizon 2020 Marie Skłodowska Curie Research and Innovation Staff Exchange ( With partners specializing in basic and applied mycology, the project researches beneficial secondary metabolites for antibiotic development and identified potential candidates for the development of eco friendly fungal biocontrol a...
Article
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Neurotrophins such as nerve growth factor (ngf) and brain-derived neurotrophic factor (bdnf) play important roles in the central nervous system. They are potential therapeutic drugs for the treatment of neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. In this study, we investigated the neurotrophic properties of tr...
Preprint
Full-text available
Neurotrophins such as nerve growth factor (ngf) and brain derived neurotrophic factor (bdnf) play important roles in the central nervous system. They are potential therapeutic drugs for treatment of neurodegerative diseases including Alzheimer's disease and Parkinson's disease. In this study, we investigated the neurotrophic properties of triterpen...
Article
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In a survey of xylarialean fungi in northern Iran, some specimens attributable to the genus Nemania were collected, cultured and sequenced. Morphological evidence and phylogenetic analyses of a combined ITS, LSU, RPB2 and TUB2 gene dataset confirmed the presence of Nemania diffusa and N. serpens in Iran for the first time. Furthermore, the new spec...
Article
Monascus species are traditional edible and medicinal filamentous fungi with a very long history in East Asia, but their sparse production of asexual spores (conidia) limits their large-scale industrial application. Previous studies have revealed that the flbA ortholog plays a negative role in the conidiation of Monascus ruber M7. FlbA, together wi...
Article
Full-text available
Corallopyronin A (CorA), a natural product antibiotic of Corallococcus coralloides, inhibits the bacterial DNA-dependent RNA polymerase. It is active against the essential Wolbachia endobacteria of filarial nematodes, preventing development, causing sterility and killing adult worms. CorA is being developed to treat the neglected tropical diseases...
Article
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The monotypic "bulbilliferous hyphomycete" genus Taxomyces was erected in 1993 for a fungal endophyte isolated from the Yew tree Taxus brevifolia and named Taxomyces andreanae. This fungus was reported to produce the plant-derived anti-cancer drug taxol. The original description of the fungus was not conclusive as to its taxonomic position because...
Article
Seven sesquiterpenoids, named nebucanes A-G (1-7), featuring a rare alliacane scaffold with unprecedented furan or pyrrole functions, were isolated from the fermentation broth of Clitocybe nebularis. Their structures were established on the basis of 1D/2D NMR spectroscopic analyses, HR-(+)-ESIMS spectra, and comparison of measured and calculated CD...
Presentation
Full-text available
Over the past decades, fungi have already yielded numerous block-buster drugs and commercial agrochemicals but many fungal species still remain to be explored systematically. However, the full potential of filamentous fungi as sources for innovative chemistry is by far not exploited exhaustively.Our mission is the search for novel anti-infective ag...
Presentation
Full-text available
Bacterial and fungal infections pose new challenges for medicine. In addition to the marked increase in the antibiotic resistance, it is the growth in so-called biofilms, which lead to chronic courses and make antibiotic treatment significantly more difficult or ineffec-tive. According to the National Institutes of Health (NIH), among all microbial...
Article
Full-text available
Five new drimane-type sesquiterpenoids were isolated from cultures of the tropical basidiomycetes, Perenniporia centrali-africana (originating from Kenya) and Cerrena sp. nov. (originating from Thailand). A new pereniporin A derivative (1), a new drimane-type sesquiterpene lactam (2), and the new 6,7-Dehydro-isodrimenediol (3) were isolated from P....
Article
Full-text available
The high global incidence of gonorrhea, the lack of a protective vaccine, and the emergence of N. gonorrhoeae strains expressing resistance to currently used antibiotics demand that new treatment options be developed. Accordingly, we investigated whether corallopyronin A, an antibiotic which is effective against other pathogens, including C. tracho...
Presentation
Full-text available
This is our poster that we presented at the AvH-DAAD conference “International Scientific Cooperation for the World of Tomorrow: Global Centres for Health and Climate”. It illustrates our joint efforts to do capacity building in Africa in terms of biodiversity reseach and drug discovery. We are very grateful for funding from the EU, the DAAD and th...
Article
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Hericium is a well-known genus that comprises edible and medicinal mushrooms with fleshy, distinctive white spines that hang from a tough, unbranched clump, and grows on dying or dead wood. In preparation for the artificial cultivation of these mushrooms in Thailand, an optimization of mycelial growth on different agar culture media, for various co...
Article
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Dicephalospora is a genus of Helotiaceae (Helotiales) that presently comprises 14 species, all of which were collected from Asia. The current study describes three additional species and a collection from Chiang Rai and Chiang Mai Provinces, Thailand. The new fungi were identified based on morphological characteristics coupled with phylogenetic ana...
Article
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In vivo studies in mice provide a valuable model to test novel active pharmaceutical ingredients due to their low material need and the fact that mice are frequently used as a species for early efficacy models. However, preclinical in vitro evaluations of formulation principles in mice are still lacking. The development of novel in vitro and in sil...
Article
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In our continued search for biologically active metabolites from cultures of rare Basidiomycota species, we found eight previously undescribed cyathane-xylosides from submerged cultures of Dentipellis fragilis, which were named dentifragilins A–H. In addition, the known cyathane deriv-atives striatal D and laxitextine A were isolated. All compounds...
Article
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The synthesis of novel disorazole C1 analogs is described and their biological activity as cytotoxic compounds is reported. Based on our convergent entry to the disorazole core we wish to present a flexible and robust strategy to construct a variety of interesting new analogs. In particular, two regions of the molecules were examined for structural...
Article
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Corallopyronin A (CorA) is active against Gram-positive bacteria and targets the switch region of RNA polymerase. Because of the high frequency of mutation (FoM) leading to rifampicin resistance, we determined the CorA FoM in S. aureus using fluctuation analysis at 4 � minimum inhibitory concentration (MIC). Resistant mutants were characterized. S....
Article
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The xylariaceous genus Dematophora has recently been resurrected and segregated from Rosellinia based on a molecular phylogeny and morphological characters. This was an important taxonomic change because Dematophora in the current sense contains several important pathogens, while Rosellinia is limited to mainly saprotrophic species that have an end...
Poster
Full-text available
Endophytes is ecolocical group of fungi which live inside plant tissues. They are actively studied as a promising source of new active secondary metabolites. In a recent study, we have isolated new bisnaphthazarins, perylenequinones, and napthopyranones derivatives from various endophytic fungi.
Article
Full-text available
Covering: August 1984 up to January 2022Worldwide, increasing morbidity and mortality due to antibiotic-resistant microbial infections has been observed. Therefore, better prevention and control of infectious diseases, as well as appropriate use of approved antibacterial drugs are crucial. There is also an urgent need for the continuous development...
Article
Full-text available
During the course of a study on the biodiversity of endophytes from Cameroon, a fungal strain was isolated. A multigene phylogenetic inference using five DNA loci revealed that this strain represents an undescribed species of Diaporthe , which is introduced here as D. breyniae . Investigation into the chemistry of this fungus led to the isolation o...
Article
Full-text available
A mycelial culture of the African basidiomycete Echinochaete cf. brachypora was studied for biologically active secondary metabolites, and four compounds were isolated from its crude extract derived from shake flask fermentations, using preparative high-performance liquid chromatography (HPLC). The pure metabolites were identified using extensive n...
Article
Full-text available
A mycelial culture of the African basidiomycete Echinochaete cf. brachypora was studied for biologically active secondary metabolites, and four compounds were isolated from its crude extract derived from shake flask fermentations, using preparative high-performance liquid chromatography (HPLC). The pure metabolites were identified using extensive n...
Article
Full-text available
Laying the groundwork on preliminary structure–activity relationship study relating to the disruptive activity of cytochalasan derivatives on mammalian cell actin cytoskeleton, we furthered our study on the cytochalasans of the Dothideomycetes fungus, Sparticola triseptata. A new cytochalasan analog triseptatin (1), along with the previously descri...
Article
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Intragenomic variation is the molecular variation within the genome among repetitive DNA. As a multigene family, nuclear ribosomal DNA (rDNA) has been widely used in fungal taxonomy for their ease in amplification and suitable variability to attain various levels of taxonomic resolution. At the intraspecific level, rDNA is believed to be under conc...
Article
Full-text available
The rise of antimicrobial resistance poses a severe threat to public health. The natural product chlorotonil was identified as a new antibiotic targeting multidrug resistant Gram-positive pathogens and Plasmodium falciparum. Although chlorotonil shows promising activities, the scaffold is highly lipophilic and displays potential biological instabil...
Article
Full-text available
The rise of antimicrobial resistance poses a severe threat to public health. The natural product chlorotonil was identified as a new antibiotic targeting multidrug resistant Gram‐positive pathogens and Plasmodium falciparum. Although chlorotonil shows promising activities, the scaffold is highly lipophilic and displays potential biological instabil...
Article
The genus Morchella has gone through turbulent taxonomic treatments. Although significant progress in Morchella systematics has been achieved in the past decade, several problems remain unresolved and taxonomy in the genus is still in flux. In late 2019, a paper published in the open-access journal Scientific Reports raised serious concerns about t...
Data
This is a point-by-point rebuttal of the paper entitled “High diversity of Morchella and a novel lineage of the esculenta clade from the north Qinling Mountains revealed by GCPSR-based study” by Phanpadith et al. (2019), published in the Open-Access journal Scientific Reports (2019, 9:19856). The present critique is published as supplementary to th...
Article
Full-text available
This paper provides an updated classification of the Kingdom Fungi (including fossil fungi) and fungus-like taxa. Five-hundred and twenty-three (535) notes are provided for newly introduced taxa and for changes that have been made since the previous outline. In the discussion, the latest taxonomic changes in Basidiomycota are provided and the class...
Article
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This is an editorial and does not have an abstract
Article
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An investigation of the chemical components of the fermentation extract of two cultures of Amylosporus cf. graminicola and Amylosporus cf. campbelii from Cuba and Zimbabwe, respectively, led to the isolation of seven previously undescribed secondary metabolites for which we proposed the trivial names amylosporanes A−G (1−7) along with the known com...
Article
inconspicuous stromatic xylarialean taxa from China, Italy, Russia, Thailand and the United Kingdom. Detailed morphological descriptions, illustrations and combined ITS-LSU-rpb2-tub2-tef1 phylogenies revealed 39 taxa from our collections belonging to Amphisphaeriales and Xylariales. A new family (Appendicosporaceae), five new genera (Magnostiolata,...
Article
Full-text available
Hericium is a genus of edible mushroom with proven medicinal efficacy. The mycelium and basidiomata contain many nutrients and bioactive compounds with therapeutic uses. Recent and emerging evidence has shown that Hericium is helpful to various diseases with medicinal properties, such as anti-oxidant, anti-cancer, anti-diabetic, anti-hyperglycemic,...
Article
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The genus Agaricus contains many edible and medicinal mushrooms; and some of them are used for commercial purposes. Most Agaricus species have been described from temperate geographic areas, but their occurrence in the tropfuics is still underexplored. The present study describes the successful cultivation of two wild strains from Thailand, A. floc...
Poster
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A small fungal bank establishment and the chemical investigation of Simplicillium subtropicum harboured in a Cameroonian medicinal plant, Duguetia staudtii
Article
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In the search for novel anti-infectives from natural sources, fungi, in particular basid-iomycetes, have proven to still harbor so much potential in terms of secondary metabolites diversity. There have been numerous reports on isolating numerous secondary metabolites from genus Laetiporus. This study reports on two new triterpenoids, laetiporins C...
Article
Full-text available
Axenic fermentation on solid rice of the saprobic fungus Sparticola junci afforded two new highly oxidized naphthalenoid polyketide derivatives, sparticatechol A (1) and sparticolin H (2) along with sparticolin A (3). The structures of 1 and 2 were elucidated on the basis of their NMR and HR-ESIMS spectroscopic data. Assignment of absolute configur...
Preprint
Full-text available
Xylariomycetidae ( Ascomycota ) is a highly diversified group with variable stromatic characters. Our research focused on inconspicuous stromatic xylarialean taxa from China, Italy, Russia, Thailand and the United Kingdom. Detailed morphological descriptions, illustrations and combined ITS-LSU- rpb 2- tub 2- tef 1 phylogenies revealed 38 taxa from...
Article
Full-text available
To date little is known about the genetic background that drives the production and diversification of secondary metabolites in the Hypoxylaceae. With the recent availability of high-quality genome sequences for 13 representative species and one relative (Xylaria hypoxylon) we attempted to survey the diversity of biosynthetic pathways in these orga...
Article
Full-text available
Eight analogues of the bioherbicides macrocidin A (1) and Z (2) with structural variance in the size of the macrocycle, its para-or meta-cyclophane character, and its functional groups were synthesized on two modular routes and tested for herbicidal, antibiotic, and antibiofilm activities. Apart from the lead compounds 1 and 2, the structurally sim...
Article
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Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera in the terminal fusarioid clade of the family Nectriaceae. Thus, an alternate hypothesis, namely a very broad concept of the genus Fusarium was proposed. In doing so, however, a significant body of data that supports distin...
Article
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Biodiversity loss from disturbances caused by human activities means that species are disappearing at an ever increasing rate. The high number of species that have yet to be described have generated extreme crisis to the taxonomist. Therefore, more than in any other era, effective ways to discover and delimitate species are needed. This paper revie...
Article
An ever-increasing demand for novel antimicrobials to treat life-threatening infections caused by the global spread of multidrug-resistant bacterial pathogens stands in stark contrast to the current level of investment in their development, particularly in the fields of natural-product-derived and synthetic small molecules. New agents displaying in...
Article
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The unambiguous application of fungal names is important to communicate scientific findings. Names are critical for (clinical) diagnostics, legal compliance, and regulatory controls, such as biosafety, food security, quarantine regulations, and industrial applications. Consequently, the stability of the taxonomic system and the traceability of nome...
Preprint
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The racemic total synthesis of nitrabirine (5) together with its previously undescribed epimer 2-epi nitrabirine (5’) is accomplished via a six-step route based on a biomimetic late-stage heterocyclization. This allowed the assignment of the relative configuration of nitrabirine by the lanthanide-induced shifts (LIS) experiment, later on confirmed...
Preprint
The racemic total synthesis of nitrabirine (5) together with its previously undescribed epimer 2-epi nitrabirine (5’) is accomplished via a six-step route based on a biomimetic late-stage heterocyclization. This allowed the assignment of the relative configuration of nitrabirine by the lanthanide-induced shifts (LIS) experiment, later on confirmed...
Preprint
Full-text available
-Accepted for publication in Studies in Mycology- To date little is known about the genetic background that drives the production and diversification of secondary metabolites in the Hypoxylaceae. With the recent availability of high-quality genome sequences for 13 representative species and one relative (Xylaria hypoxylon) we attempted to survey t...
Article
Full-text available
Background The dieback epidemic decimating common ash ( Fraxinus excelsior ) in Europe is caused by the invasive fungus Hymenoscyphus fraxineus . In this study we analyzed the genomes of H. fraxineus and H. albidus , its native but, now essentially displaced, non-pathogenic sister species, and compared them with several other members of Helotiales....
Article
Sparticols A (1) and B (2), two catechol-bearing naphthalenedioxy derivatives, were isolated from the submerged culture of the Spanish broom inhabiting Dothideomycetes fungus, Sparticola junci. The structures of 1 and 2 were established by NMR spectroscopic analysis and high-resolution mass spectrometry. The 8S absolute configuration of their β-hyd...
Article
Full-text available
Morphological characters and multi-gene phylogenetic analyses were used to identify Retiboletus specimens collected in northern Thailand. Retiboletus brevibasidiatus is described as new to science, whereas R. fuscus and R. nigrogriseus are reported for the first time from Thailand. Retiboletus brevibasidiatus produces medium-sized basidiomes, with...
Article
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A recent find of a Marasmius species in Northern Thailand led to the isolation of five unprecedented derivatives of the carotane antibiotic fulvoferruginin (1), fulvoferruginins B-F (2-6). The structures of these sesquiterpenoids were elucidated using HRESIMS, 1D and 2D NMR, as well as CD spectroscopy. Assessing the bioactivity, fulvoferruginin eme...
Article
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The new genus Morinagamyces is introduced herein to accommodate the fungus Apiosordaria vermicularis as inferred from a phylogenetic study based on sequences of the internal transcribed spacer region (ITS), the nuclear rDNA large subunit (LSU), and partial fragments of ribosomal polymerase II subunit 2 (rpb2) and β‐tubulin (tub2) genes. Morinagamyc...
Article
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This mini-review is dedicated to the summary of results of the EU-funded Project “Golden Mycological Triangle” (acronym GoMyTri), which was carried out in collaboration of three research infrastructures in Germany, the Netherlands and Thailand during the years 2014-2018. The cooperation explored the mycological and microbiological biodiversity of E...
Article
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Submerged mycelial cultures of the ascomycete Colpoma quercinum CCTU A372 were found to produce five previously undescribed tetramic acids, for which we propose the trivial names colposetins A–C (1–3) and colpomenoic acids A and B (4 and 5), along with the known compounds penicillide (6) and monodictyphenone (7). The planar structures of 1–5 were d...
Article
The fungal macrolide berkeleylactone A was synthesised in 13 steps and 24% yield using (R)-propylene oxide and an asymmetric Noyori hydrogenation of a β-ketoester to install the stereogenic centres. A domino addition-Wittig olefination of a 13-hydroxytetradecanal intermediate with the cumulated ylide Ph3PCCO closed the macrocyle by establishing the...
Article
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It is now a decade since The International Commission on the Taxonomy of Fungi (ICTF) produced an overview of requirements and best practices for describing a new fungal species. In the meantime the International Code of Nomenclature for algae, fungi, and plants (ICNafp) has changed from its former name (the International Code of Botanical Nomencla...