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Marc Debrunner currently works at the Institut für Molekulare Biologie , University of Zurich. Marc does research in Developmental Biology, Cell Biology and Neuroscience. Their most recent publication is 'CRISPR-induced double-strand breaks trigger recombination between homologous chromosome arms'.
CRISPR–Cas9–based genome editing has transformed the life sciences, enabling virtually unlimited genetic manipulation of genomes: The RNA-guided Cas9 endonuclease cuts DNA at a specific target sequence and the resulting double-strand breaks are mended by one of the intrinsic cellular repair pathways. Imprecise double-strand repair will introduce ra...
Background Axons navigate to their future synaptic targets with the help of choice points, intermediate targets that express axon guidance cues. Once they reach a choice point, axons need to switch their response from attraction to repulsion in order to move on with the next stage of their journey. The mechanisms underlying the change in axonal res...
Figure S1. Dorsal electroporation of dsRabGDI results in the same phenotype as one-sided electroporation. Targeting dsRNA derived from RabGDI only into the dorsal spinal cord reproduced the phenotype seen after electroporation of one side of the spinal cord (A). A majority of growth cones stalled in the floor plate (arrows in B). Aberrant axonal pa...
Figure S2. Downregulation of RabGDI does not interfere with spinal cord patterning. Transverse sections of HH23 embryos were stained with Pax3 (A and B), Nkx2.2 (C and D), and Isl-1 (E and F) to assess spinal cord patterning. The electroporation of dsRabGDI did not change the expression of Pax3 (B), Nkx2.2 (D), or Isl-1 (F). Inserts in B,D, and F s...
Extracellular Slits and their transmembrane receptors, the Robos, represent a well characterized repulsive guidance system which is involved in different processes of axon guidance and migration of cells in invertebrates and vertebrates. In my dissertation, we dissected the role of Slits and Robos in axon guidance of sensory and motoneurons and the...