About
70
Publications
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Introduction
Additional affiliations
January 2018 - July 2018
Position
- PostDoc Position
Description
- Impact of warming and chlorpyrifos on coral-reef fish larvae fitness
September 2014 - October 2017
Position
- PhD Student
Description
- Importance of metamorphosis in coral-reef fish larval recruitment facing anthropogenic stressors
July 2018 - July 2020
Education
September 2012 - June 2014
September 2010 - June 2011
September 2008 - August 2010
Lycée Saint-Louis, Paris, France
Field of study
- Classe préparatoire BCPST (Biologie Chimie Physique et Science de la Terre)
Publications
Publications (70)
A large majority of coral reef fish species have a biphasic life cycle, which starts with a planktonic larval phase in the open ocean followed by larval recruitment within the reef, with a subsequent development to the juvenile and adult stage. This ecological transition is associated with profound morphological, physiological, and behavioral chang...
Biodiversity faces unprecedented threats from rapid global change¹. Signals of biodiversity change come from time-series abundance datasets for thousands of species over large geographic and temporal scales. Analyses of these biodiversity datasets have pointed to varied trends in abundance, including increases and decreases. However, these analyses...
Temperature change affects biological systems in multifaceted ways, including the alteration of species interaction strengths, with implications for the stability of populations and communities. Temperature-dependent changes to antipredatory responses are an emerging mechanism of destabilization and thus there is a need to understand how prey speci...
Temperature is a fundamental driver of species' vital rates and thus coexistence, extinctions and community composition. While temperature is neither static in space nor in time, little work has incorporated spatiotemporal dynamics into community‐level investigations of thermal variation.
We conducted a microcosm experiment using ciliate protozoa t...
The present study reports the first experimental microplastic-mediated transfer of a key PCB congener into adult specimens of the sea urchin Paracentrotus lividus. Three experiments were conducted to assess whether 14C-PCB- 153 adsorbed onto negatively buoyant microplastics (MPs) (500–600 μm) is bioavailable to the sea urchin: (1) exposure to a low...
In marine environments, mutualisms such as those between corals or sea anemones and their algal symbionts (Symbiodiniaceae) play a key role for supporting surrounding biodiversity. However, as the breakdown of the mutualism between corals and/or anemones and Symbiodiniaceae (i.e. bleaching) become increasingly frequent and severe, the risk of losin...
In the face of rapid global change and an uncertain fate for biodiversity, it is vital to quantify trends in wild populations. These trends are typically estimated from abundance time series for suites of species across large geographic and temporal scales. Such data implicitly contain phylogenetic, spatial, and temporal structure which, if not pro...
High‐resolution monitoring is fundamental to understand ecosystems dynamics in an era of global change and biodiversity declines. While real‐time and automated monitoring of abiotic components has been possible for some time, monitoring biotic components—for example, individual behaviours and traits, and species abundance and distribution—is far mo...
The fate and toxicity of ingested marine microplastics (MPs) has been a major concern in aquatic ecotoxicology for the last decade. Although their ingestion by a wide range of marine organisms has been proven, the uptake of MPs within organs is not yet fully understood and relies on the ability of ingested microplastics to transfer from the gut to...
LINK TO THE PEER-REVIEWED & PUBLISHED ARTICLE:
https://www.researchgate.net/publication/364472513_Towards_the_fully_automated_monitoring_of_ecological_communities
High resolution monitoring is fundamental to understand and predict the dynamics of ecological communities in an era of global change and biodiversity declines. While real-time and fully...
Environmental microplastic particles (MPs) represent a potential threat to many aquatic animals, and experimental exposure studies, when done well, offer a quantitative approach to assess this stress systematically and reliably. While the scientific literature on MP studies in aquatic environments is rapidly growing, there is still much to learn, a...
Plastic pollution has become a major environmental and societal concern in the last decade. From larger debris to microplastics (MP), this pollution is ubiquitous and particularly affects aquatic ecosystems. MP can be directly or inadvertently ingested by organisms, transferred along the trophic chain, and sometimes translocated into tissues. Howev...
Advancement and diversification of the aquaculture industry is reliant on the development of captive breeding and rearing protocols for novel fish species. Orbicular batfish (Platax orbicularis), a major emerging species in Polynesian aquaculture, live in brackish and marine waters around coral reefs, and are highly prized by Pacific Island communi...
Larval metamorphosis and recruitment represent critical life-history transitions for most teleost fishes. While the detrimental effects of anthropogenic stressors on the behavior and survival of recruiting fishes are well-documented, the physiological mechanisms that underpin these patterns remain unclear. Here, we use pharmacological treatments to...
Plastic pollution has become a major environmental concern worldwide, and marine ecosystems have become polluted with ubiquitous microplastic particles (MP). MP can contain chemical additives and can also scavenge pollutants from the surrounding environment, and these co-contaminants may threaten the marine biota when MP become inadvertently ingest...
French Polynesia exhibits a wide diversity of islands and coral-reef habitats, from urbanized high islands to remote atolls. Here, we present a geographically extensive baseline survey that examine the concentrations of nine metals (Ag, Cd, Co, Cu, Fe, Mn, Ni, Pb and Zn) and one metalloid (As) in superficial sediments from 28 sites spread over thre...
Changes in seawater pH can alter the chemical speciation of waterborne chemical elements, affecting their bioavailability and, consequently, their bioaccumulation in marine organisms. Here, controlled environmental conditions and a 210 Pb radiotracer were used to assess the effect of five distinct pH conditions (pH T ranging from 7.16 to 7.94) on t...
How vocal organisms share acoustic space has primarily received attention in terrestrial environments. Comparable studies in marine environments, however, remain rare. By recording sounds on a coral reef in French Polynesia for 48 h and 24 h, this study provides first insights on how different sound types are distributed within the acoustic space a...
Marine environments are far from being silent and coral reefs in particular house a large variety of vocal fishes. How can the different species communicate efficiently and avoid cacophony? Fishes from French Polynesia may optimize their communication by selecting specific time and spectral windows to insert their signals within the acoustic space....
Environmental plastic pollution is a major ecological and societal concern today. Over the last decade, a broad range of laboratory and experimental studies have complemented field observations in the hope of achieving a better understanding of the fate and impact of micro- and/or nano-plastics (MP/NP) on diverse organisms (e.g. birds, fish and mam...
Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance gene...
Artificial light at night (ALAN) is a recently acknowledged form of anthropogenic pollution of growing concern to the biology and ecology of exposed organisms. Though ALAN can have detrimental effects on physiology and behaviour, we have little understanding of how marine organisms in coastal areas may be impacted. Here, we investigated the effects...
Microplastics (MP) are ubiquitous in the marine environment and have been shown to alter the behaviour of some species due to potential neurotoxic effect. However, very little is known on the effect of this stressor on behavioural responses of early and more vulnerable life stages. This study explores the effects of polystyrene MP (90 µm diameter)...
Mutualisms are important ecological interactions that underpin much of the world's biodiversity. Predation risk has been shown to regulate mutualism dynamics in species‐specific case studies; however, we lack studies which investigate whether predation can also explain broader patterns of mutualism evolution. We report that fish‐anemone mutualisms...
Understanding the relationship between coral reef condition and recruitment potential is vital for the development of effective management strategies that maintain coral cover and biodiversity. Coral larvae (planulae) have been shown to use certain sensory cues to orient towards settlement habitats (e.g. the odour of live crustose coralline algae -...
Dory, the animated surgeonfish created by the Pixar Animation studios, famously suffered from short-term memory loss leading to many adventures. In reality, many fishes have excellent cognitive abilities and are able to learn and retain important information such as the identity of predators. However, if and how cognition can be affected by anthrop...
Herein, we describe the isolation and spectroscopic identification of eight new tetrabrominated tyrosine alkaloids 2-9 from the Polynesian sponge Suberea ianthelliformis, along with known major compound psammaplysene D (1), N,N-dimethyldibromotyramine, 5-hydroxy xanthenuric acid, and xanthenuric acid. Cytotoxicity and acetylcholinesterase inhibitio...
Herein, we describe the isolation and spectroscopic identification of eight new tetrabrominated tyrosine alkaloids 2-9 from the Polynesian sponge Suberea ianthelliformis, along with known major compound psammaplysene D (1), N,N-dimethyldibromotyramine, 5-hydroxy xanthenuric acid, and xanthenuric acid. Cytotoxicity and acetylcholinesterase inhibitio...
Teleost fish represent the most diverse of the vertebrate groups and play important roles in food webs, as ecosystem engineers, and as vectors for microorganisms. However, the microbial ecology of fishes remains underexplored for most host taxa and for certain niches on the fish body. This is particularly true for the gills, the key sites of respir...
Color polymorphism is widespread in animals and can be associated with temporary adjustments to environmental variables (phenotypic plasticity). In teleost fishes, one of the most remarkable examples of color plasticity is background adaptation in flatfishes. However, such rapid and complete changes in body color and pattern remain relatively unrep...
Acoustic recording has been recognized as a valuable tool for non-intrusive monitoring of the marine environment, complementing traditional visual surveys. Acoustic surveys conducted on coral ecosystems have so far been restricted to barrier reefs and to shallow depths (10–30 m). Since they may provide refuge for coral reef organisms, the monitorin...
Larval recruitment, the transition of pelagic larvae into reef-associated juveniles, is a critical step for the resilience of marine fish populations but its molecular control is unknown. Here, we investigate whether thyroid-hormones (TH) and their receptors (TR) coordinate the larval recruitment of the coral-reef-fish Acanthurus triostegus. We dem...
Acanthurus triostegus weight at different developmental stages.
Acanthurus triostegus intestine length at different developmental stages.
Acanthurus triostegus T4 levels in larvae and juveniles.
TR and Klf9 expression levels in Acanthurus triostegus at different developmental stages (qPCR).
T3 levels in external slope relocated Acanthus triostegus.
TR and Klf9 expression levels in external slope relocated Acanthurus triostegus (qPCR).
Acanthurus triostegus grazing activity under pharmacological treatments or relocation.
Grazing activity of Acanthurus triostegus under different chlorpyrifos exposure conditions.
Transactivation assay of Acanthurus triostegus TR for several thyroid hormone derivatives.
T3 levels in Acanthurus triostegus under different chlorpyrifos exposure conditions.
Acanthurus triostegus T3 levels in larvae and juveniles.
Transactivation assay of Acanthurus triostegus TR for the competition of T3 and NH3.
List of primers used in this study.
T4 levels in external slope relocated Acanthus triostegus.
Quantity of grazed algae by Acanthurus triostegus under different chlorpyrifos exposure conditions.
T4 levels in Acanthurus triostegus under different chlorpyrifos exposure conditions.
List of TR used for phylogenetic reconstruction.
Acanthurus triostegus intestine length under pharmacological treatments or relocation.
T4 and T3 levels of various coral reef fishes at different development stages.
Intestine length of Acanthurus triostegus under different chlorpyrifos exposure conditions.
Marine sponges of the genus Suberea (order: verongida) are known to be prolific source of a large array of structurally diverse bromotyrosine alkaloids. These alkaloids are exceedingly abundant in verongida order.[1-3] In this work, eight new tetrabrominated tyrosine alkaloids were isolated from the French Polynesian sponge Suberea ianthelliformis,...
Lateralization, i.e. the preferential use of one side of the body, may convey fitness benefits for organisms within rapidly-changing environments, by optimizing separate and parallel processing of different information between the two brain hemispheres. In coral reef-fishes, the movement of larvae from planktonic to reef environments (recruitment)...
Sponges of the marine genus Suberea (order: verongidea) are known to produce a large array of structurally diverse brominated tyrosine alkaloids, which considered chemotaxonomical markers. eight new tetrabrominated tyrosine alkaloids were isolated from the French Polynesian sponge Suberea ianthelliformis, along with the known natural products psamm...
For marine fishes with a bipartite life cycle, pelagic larval dispersal can shape the distribution, connectivity, composition and resilience of adult populations. Numerous studies of larval dispersal, and associated settlement and recruitment processes, have examined the relationship between population connectivity and oceanographic features. Howev...
Collected larval fishes and percentage of the moon that was illuminated.
Raw data of the larval fishes that were collected during the study and data of the percentage of the moon that was illuminated.
(XLSX)
The replenishment and persistence of marine species is contingent on dispersing larvae locating suitable habitat and surviving to a reproductive stage. Pelagic larvae rely on environmental cues to make behavioural decisions with chemical information being important for habitat selection at settlement. We explored the sensory world of crustaceans an...
In the European eel (Anguilla anguilla, L.), the steep decline of reproductive silver eels is partly due to disorientation and mortality during their downstream migration, when facing turbines, but also reservoirs and dams. In the Frémur, an obstructed river in Brittany, which is representative of the western coastal hydrosystem of France, five hyd...
In vertebrates, brain functional asymmetries are widespread and increase brain performance. Some species of fishes are known to have brain asymmetries; however, little information is available on brain lateralization in coral reef fishes and the impact this could have during the recruitment phase. In this study, soldierfish, Myripristis pralinia, a...
As it is unlikely that successful settlement is solely a matter of chance (i.e. to find a suitable habitat), one of the greatest challenges facing the fish larvae is how to locate the relatively rare patches of coral reef habitat on which they settle and ultimately reside as adults. The answer must lie partly in the sensory modalities of fishes at...
River management is known to have severely fragmented river continuity. The steep decline of temperate eels is caused by a set of factors including dams that reduce accessibility to growth habitats and induce turbine mortality. We studied effects of dams and reservoir on downstream migration behaviour of silver European eels. We used an acoustic te...
Questions
Questions (3)
Hi,
I'm looking for the proper way to analyze some of my data.
I studied predation in fish, by looking at survival rates of different groups of larval fishes when facing predators.
In the first trial of the experiment, I put 10 larval fishes of the group A, 10 larval fishes of the group B, and 10 larval fishes of the group C, all together in a predation arena.
After 2 hours:
Group A had 10 survivors
Group B had 8 survivors
Group C had 6 survivors
In the second trial, same procedure.
Group A had 5 survivors
Group B had 4 survivors
Group C had 3 survivors
etc, multiple times.
The issue here is that predators are sometimes very hungry (see the second trial) and sometimes not that much (see thr first trial) so calculating a "survival mean" for each group, among all the trials, makes no sense to me (lot of variability).
So what I did is calculating the "overall survival rate" in each trial.
Overall survival in trial 1 : 0.8
Overall survival in trial 2 : 0.4
Then I substracted this "overall survival" to each group survival, in order to create some sort of an index that "normalizes" each survival rate according to the "overall predation" in each trial.
So this gives me the following:
In the 1st trial
Group A survival index = 1 - 0.8 = 0.2
Group B survival index = 0.8 - 0.8 = 0
Group C survival index = 0.6 - 0.8 = -0.2
In the second trial
Group A survival index = 0.5 - 0.4 = 0.1
Group B survival index = 0.4 - 0.4 = 0
Group C survival index = 0.3 - 0.4 = -0.1
So when I calculate the mean of these indexes for each fish groups among all the trials, I end up with something nice : group A survival index is always positive, group B around 0, and group C always negative, so low variability and nice means that suggest that group A survived more than group B, and group B survived more than group C.
But now I would like to test this statistically, and I can't find out how to compare multiple paired data of that type. I usually do my stats in R.
If anyone has suggestions I'll be more than happy.
Thank you very much for those who read me up to here, and thanks in advance for those who'll be able to help :)
Cheers
Marc
Hi everyone,
I'm looking for a little help in the analysis of my qPCR raw results. I hope you'll be able to help me. Thanks in advance.
Here is my example:
I have 3 genes: 1 target gene, and 2 housekeeping genes.
I have estimated the PCR efficiency for these genes and their associated primers: E(target gene) = 2.1, E(HK gene 1) = 1.9, E(HK gene 2) = 2.2
I have Ct raw results for 2 technical replicates each time
I have a Treatment condition, and a Control condition, for all of the 3 genes (target gene and the 2 housekeeping genes)
Finally I have these data for 3 biological replicates
(see the attach Excel document).
From these data I have calculated the normalized expression of my target gene (using the control condition as a calibrator, and the housekeeping genes for final normalization). All the calculations are in the Excel document.
In the end I have a mean of the normalized expression for my target gene, which is great. However, I don't know yet how to obtain the standard error for this mean. I can calculate the standard error from the 3 normalized expressions from my 3 biological replicate (SE of 1.39, 1.55 and 1.26, which is easy in R or Excel), but from I what I read on internet (but I didn't understand it yet) there's apparently a way to calculate the SE value from the different SE obtained all along the calculation process (SE from the mean of the technical replicate values of CT, then SE from the relative quantity calculation, and finally SE from the normalization process).
Does anyone is familiar with that and could provide a little help or at least a little look at the attached document to confirm that my calculations are correct. From then you'll probably be able to lead me towards the correct calculations for my SE value.
Thanks a lot in advance
Regards
Marc
I'd like to observe fish lateral line and more specifically neuromasts and hair cells. For that I can use SEM Hitashi TM3030.
Usually I use the following protocol: 1/ fixation in glutaraldehyde, 2/ post-fixation in osmium, 3/ dehydration in EtOH, 4/ drying in HMDS and 5/ metallization, before SEM observation.
Apparently, with SEM Hitashi TM3030, there is no need of post-fixation in osmium and metallization. Has anyone already performed tissue obervation with such device? Which protocol did you use to prepare your sample?
Thanks!
