Manuel Toledano

• Managing Director at University of Granada

Aim To evaluate in a laboratory setting the antimicrobial properties and the potential to inhibit biofilm formation of novel remineralizing polymeric nanoparticles (NPs) when applied to dentine surfaces and to ascertain the effect of the functionalization of these NPs with zinc, calcium or doxycycline. Methodology The antimicrobial activity and in...

Objective: The purpose of this in vitro study was to evaluate the antibacterial effect of a novel non-resorbable, bioactive polymeric nanostructured membrane (NMs), when doped with zinc, calcium and doxycycline. Methods: A validated in vitro subgingival biofilm model with six bacterial species (Streptococcus oralis, Actinomyces naeslundii, Veill...

The invention relates to a hydrophilic nonwoven nanofiber membrane based on acrylate and methacrylate copolymers and its process of preparation. Furthermore, the invention refers to its hydrolysed form further functionalised with a divalent cation selected from Zn+2, Ca+2, Mg+2 and Sr+2, an antibacterial agent and any of the combinations thereof. M...

Objective: Nanogels designing for effective treatment of eroded cervical dentin lesions. Materials and methods: Polymethylmetacrylate-based nanoparticles (NPs) were doxycycline (D), calcium, or zinc loaded. They were applied on eroded cervical dentin. Treated surfaces were characterized morphologically by atomic force and scanning electron micro...

Background The aim was to evaluate the effect of silver loaded nanoparticles (NPs) application on the triboscopic, crystallographic and viscoelastic properties of demineralized dentin. Polymethylmetacrylate-based NPs and Ag loaded NPs were applied on demineralized dentin. Material and Methods Treated and untreated surfaces were probed by a nanoind...

The present invention relates to a method of precipitation polymerization to obtain polymeric spherical particles of copolymer with a statistical topology and chemical structure (acid monomer selected from acrylic or methacrylic)-co-(cross-linking agent selected from acrylic or methacrylic)-co-(hydroxylated monomer selected from acrylic or methacry...

The aim of this research was to ascertain the mechanical and chemical behavior of sound and caries-affected dentin (CAD), after the placement of Zn-free vs containing amalgam restorations. Peritubular and intertubular dentin were evaluated using, a) nanoindenter in scanning mode; the load and displacement responses were used to perform the nano-Dyn...

Objectives: Biomaterials for treating dentin hypersensitivity and dentin wear were evaluated to efficiently occlude the dentinal tubules and to increase dentin resistance to abrasion. Materials and methods: Twenty-four dentin surfaces were treated with EDTA to expose dentinal tubules and were (1) non-brushed, (2) brushed with distilled water, or...

Background The purpose of this study was to evaluate nanohardness and viscoelastic behavior of dentin surfaces treated with two canal sealer cements for dentin remineralization. Material and Methods Dentin surfaces were subjected to: i) 37% phosphoric acid (PA) or ii) 0.5 M ethylenediaminetetraacetic acid (EDTA) conditioning prior to the applicati...

Objective: to study the antibacterial effect of polymeric PolymP-n Active nanoparticles using an in vitro subgingival biofilm model. Methods: Hydroxyapatite discs coated with five modalities of nanoparticles (NPs): NPs, NPs doped with zinc, calcium, silver and doxycycline, PBS as control, and Streptococcus oralis, Actinomyces naeslundii, Veillon...

Objective: This study aimed to evaluate the effect of silver loaded nanoparticles (NPs) application on dentin remineralization. Methods: Polymethylmetacrylate-based NPs and silver loaded NPs (Ag-NPs) were applied on demineralized dentin surfaces. Dentin was characterized morphologically by scanning electron microscopy, mechanically probed by a n...

Background: The inner structure of trabecular bone is a result of structural optimization provided by remodeling processes. Changes in hormonal status related to menopause cause bone tissue loss and micro-architectural deterioration with a consequent susceptibility to fracture. Accumulation of micro-damage in bone, as a function of the rate of pro...

Background: Improvements to the understating of the compositional contributions of bone mineral and organic components to the competence of trabecular bone are crucial. The purpose of this study was to propose a protocol to study biochemical composition of trabecular bone, based on two combined Raman analysis methodologies. Material and methods:...

Polymeric nanoparticles were modified to exert antimicrobial activity against oral bacteria. Nanoparticles were loaded with calcium, zinc and doxycycline. Ions and doxycycline release were measured by inductively coupled plasma optical emission spectrometer and high performance liquid chromatography. Porphyromonas gingivalis, Lactobacillus lactis,...

Objective: The aim of this study was to evaluate the induced changes in the chemical and mechanical performance at the glass-ionomer cement-dentin interface after mechanical load application. Methods: A conventional glass-ionomer cement (GIC) (Ketac Bond), and a resin-modified glass-ionomer cement (RMGIC) (Vitrebond Plus) were used. Bonded inter...

Statement of significance: Erosion from acids provokes dentin hypersensitivity (DH) which presents with intense pain of short duration. Open dentinal tubules and demineralization favor DH. Nanogels based on Ca-nanoparticles and Zn-nanoparticles produced an efficient reduction of fluid flow. Dentinal tubules were filled by precipitation of induced...

Aim: To investigate if crystallinity and ultrastructure is modified when cervical dentine is treated with four different nanogels-based solutions for remineralizing purposes. Methodology: Experimental nanogels based on polymeric nanoparticles (NPs) and zinc, calcium, or doxycycline-loaded NPs were applied on citric acid etched dentine to facilit...

Objective The objective of the study is characterization of novel calcium and zinc-loaded electrospun matrices to be used for periodontal regeneration. Materials and methodsA polymethylmetacrylate-based membrane was calcium or zinc loaded. Matrices were characterized morphologically by atomic force and scanning electron microscopy and mechanically...

Continuing tissue destruction in osteoarthrosis is maintained by molecular pathways related to an unbalanced chondrocyte metabolism, the loss of reactive oxygen species (ROS) homeostasis, increase catabolism in a degraded matrix and the limited response to growth factors due to cell aging. Rare deleterious gene variants driving relevant molecular p...

The possibility of diagnosis and prediction of multiple disorders in trabecular bone through nano-biomechanics and chemical analysis are summarized. Improvements to the understating of the compositional contributors of bone mineral and organic components to mechanical competence are crucial. Viscoelastic properties and Raman characterization have b...

Two types of PHEMA-based particles, exhibiting either carboxyl or tertiary ammine functional groups, were incorporated to fibrin-agarose hydrogels, and the effect of the addition of these synthetic particles on the viscoelastic and microstructural properties of the biomaterials was evaluated. Experimental results indicated that the incorporation of...

Aim: To evaluate the remineralization ability of two endodontic sealer cements. Methodology: Mid-coronal dentine surfaces were subjected to: i) 37% phosphoric acid (PA) or ii) 0.5 M ethylenediaminetetraacetic acid (EDTA) conditioning prior to the application of two experimental hydroxyapatite-based cements, containing sodium hydroxide (calcypati...

The aim of this study was to evaluate the changes in the mechanical behavior and bonding capability of Zn-doped resin-infiltrated caries-affected dentin interfaces. Dentin surfaces were treated with 37% phosphoric acid (PA) followed by application of a dentin adhesive, single bond (SB) (PA+SB) or by 0.5 M ethylenediaminetetraacetic acid (EDTA) foll...

The aim of this study was to ascertain the effect of Zn-doping of dental adhesives and mechanical load cycling on the micromorphology of the resin-dentin interdiffusion zone (of sound and caries affected dentin). The investigation considered two different Zn-doped adhesive approaches and evaluated the interface using a doubled dye fluorescent techn...

The aim of this study was to investigate if load cycling affects interfacial integrity of glass ionomer cements bonded to sound- or caries-affected dentin. A conventional glass ionomer, Ketac Bond, and a resin-modified glass ionomer (Vitrebond Plus), were applied to dentin. Half of the specimens were load cycled. The interfaces were submitted to dy...

The aim of this study was to evaluate changes in the mechanical and chemical behavior, and bonding ability at dentin interfaces infiltrated with polymeric nanoparticles (NPs) prior to resin application. Dentin surfaces were treated with 37% phosphoric acid followed by application of an ethanol suspension of NPs, Zn-NPs or Ca-NPs followed by the app...

Aesthetic and plastic restorative materials in dentistry are demanded. These materials are resin based, and require demineralization procedures on dentine and enamel, prior to resin application. Infiltration of hydrophilic monomers into demineralized tissue creates a hybrid layer that couples resinous materials to dentine. Resin infiltration is nev...

IntroductionDemineralized collagen fibers at the hybrid layer are susceptible to degradation. Remineralization may aid to improve bond longevity. Objectives The aim of the present study was to infiltrate zinc and calcium-loaded polymeric nanoparticles into demineralized dentin to facilitate hybrid layer remineralization. Materials and methodsZinc o...

Aims to design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease. Methods PolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy...

Excel file with Ca and Zn complexation data. (XLSX)

Excel file with Cell viability data. (XLSX)

The aim of this study was to assess the mechanical and chemical performance of sound and caries-affected dentin, after Zn-free versus containing amalgam restorations placement and thermocycling. Dentin surfaces were studied by Atomic Force Microscopy analysis for surface morphological characterization (including fibril diameter assessment), nano-in...

The aim of this research was to assess the ability of amalgam restorations to induce amorphous mineral precipitation at the caries-affected dentin substrate. Sound and caries-affected dentin surfaces were subjected to both Zn-free and Zn-containing dental amalgam restorations. Specimens were submitted to thermocycling (100,000 cycles/5°C–55°C, 3 mo...

The aim of this study was to ascertain the crystal morphology and to assess the ultrastructure and texture changes of sound (SD) and caries-affected dentin (CAD) after being restored with Zn-free and Zn-containing amalgam. Dentin surfaces were studied before amalgam placement and after amalgam removal by X-ray diffraction (μXRD2) and transmission e...

The aim of this study was to evaluate if mechanical cycling influences bioactivity and bond strength at the glass-ionomer cement-dentin interface, after load cycling. Microtensile bond strength (MTBS) was assessed with Ketac-Bond (conventional glass ionomer/GIC) or Vitrebond Plus (resin-modified/RMGIC), in sound dentin or in caries-affected dentin...

Objectives: The aim of this study was to evaluate the effect of bioactive glass–ceramic particles (Biosilicate®) addition on surface nanoroughness and topography of Resin-modified glass ionomer cements (RMGICs). Methods: Experimental materials were made by incorporating 2 wt% of Biosilicate® into Fuji II LC® (FL) and Vitremer® (VT) powders. Disks o...

The aim of this research was to assess the influence of mechanical loading on the ability of Zn-free versus Zn -containing amalgams to promote remineralization at the dentin interface. Sound and caries-affected dentin surfaces (CAD) were restored using Zn-free or Zn-containing dental amalgams. Midcoronal dentin surfaces were studied by (1) atomic f...

The purpose of this study was to investigate micro-morphology of the resin-dentin inter-diffusion zone using two different single-bottle self-etching dentin adhesives with and without previous acid-etching, after in vitro mechanical loading stimuli. Extracted human third molars were sectioned to obtain dentin surfaces. Two different single-bottle s...

Purpose: To evaluate changes in the physicochemical properties, water sorption (WS), solubility (SO), modulus of elasticity (E), ultimate tensile strength (UTS), and microhardness (MH) tests were undertaken in zinc-doped dental adhesives. Methods: Two bonding resins, Adper Single Bond Plus (SB) and Clearfil SE Bond (SEB), were zinc-doped by mixi...

The aim of this study was to evaluate the induced changes in mechanical behavior and bonding capability of resin-infiltrated dentine interfaces, after application of mechanical stimuli. Dentine surfaces were subjected to partial demineralization through 37% phosphoric acid etching followed by the application of an etch-and-rinse dentine adhesive, S...

The aim of this study was to evaluate if mechanical cycling influences bioactivity at the resin-carious dentin interface after bonding with Zn-doped self-etching adhesives. Caries-affected dentin surfaces were bonded with: Clearfil SE bond (SEB), and 10 wt. % ZnO nanoparticles or 2 wt. % ZnCl2 were added into the SEB primer or bonding components. B...

To ascertain if biomimetic phosphoprotein analogues (polyaspartic acid -PAS- and sodium trimetaphosphate -TMP-) improve bonding efficacy and dentine remineralization ability of a novel zinc-doped Portland-based resinous sealing cement. Bonding procedures were performed on phosphoric acid etched dentine and several groups were established regarding...

This chapter provides a tool for the integration of new concepts and biomaterials related with the resin-dentine interface. The principles of dentine demineralisation and remineralisation that shape modern restorative dentistry practices, as well as considerations for the selection of new materials for different restorative approaches, are emphasis...

The aim of this study was to evaluate if mechanical cycling influences bioactivity and bond strength of resin-dentine interface after bonding with Zn-doped self-etching adhesives. Sound dentine surfaces were bonded with Clearfil SE Bond (SEB), 10wt% ZnO microparticles or 2wt% ZnCl2 were added into the SEB primer (P) or bonding (Bd) for Zn-doping. B...

Bioactive glasses are surface-active and able to induce remineralization of dentin. Two resin-modified glass-ionomer cements (RMGICs) doped with bioactive glass (Biosilicate®) were used as restorative materials in dentin. Experimental powders were made by incorporating 2, 5, and 10 wt% of Biosilicate® in Vitremer® (VT) and Fuji II LC® (FL) powders....

Objective: The study aimed at evaluating the remineralization of acid-etched dentin pre-treated with primers containing biomimetic analogs and bonded using an ion-releasing light-curable resin-based material. Methods: An experimental etch-and-rinse adhesive system filled with Ca(2+), PO4(3-)-releasing Ca-Silicate micro-fillers was created along...

The purpose of this study was to evaluate if mechanical loading influences bioactivity and bond strength at the resin-dentin interface after bonding with Zn-doped etch-and-rinse adhesives. Dentin surfaces were subjected to demineralization by 37% phosphoric acid (PA) or 0.5 M ethylenediaminetetraacetic acid (EDTA). Single bond (SB) adhesive-3M ESPE...

The purpose of this study was to evaluate if different in vitro functional and parafunctional habits promote mineralization at the resin-dentin interface after bonding with three different adhesive approaches. Dentin surfaces were subjected to distinct treatments: demineralization by (1) 37% phosphoric acid (PA) followed by application of an etch-a...

Introduction Matrix metalloproteinase (MMP) inhibition may improve endodontic treatment prognosis. The purpose of this study was to determine if zinc incorporation into experimental resin cements containing bioactive fillers may modulate MMP-mediated collagen degradation of dentin. Methods Human dentin samples untreated and demineralized using 10%...

To obtain more durable adhesion to dentin, and to protect collagen fibrils of the dentin matrix from degradation, calcium- and phosphate-releasing particles have been incorporated into the dental adhesive procedure. The aim of the present study was to incorporate zinc-loaded polymeric nanocarriers into a dental adhesive system to facilitate inhibit...

Objectives: The purpose was to evaluate physico-mechanical and morphological changes of demineralized dentin at early stages of remineralization. Methods: Dentin surfaces were processed: (1) untreated dentin - UD, (2) 37% phosphoric acid application 15s (partially demineralized dentin-PDD) and (3) 10% phosphoric acid 12h (totally demineralized den...

Objective: to ascertain whether zinc may improve the repair ability of demineralized dentin. Methods: Dentin disks were demineralized by phosphoric acid during 15 s and immersed in artificial saliva (AS), remineralizing solution (RS), a zinc chloride solution and a zinc oxide solution. Dentin specimens were analyzed after 24 h and 1 month of stora...

Objective: This study evaluated the ability of different in vitro mechanical loading tests to promote new mineral formation at the bonded dentine interfaces created with a two-step self-etching resin adhesive. Methodology: Restored teeth were divided in the following groups: (1) unloaded, load cycling with (2) sine waveform, (3) square waveform,...

The effect of hydration on the biomechanical properties of fibrin and fibrin-agarose tissue-like hydrogels is reported. Native hydrogels with approximately 99.5% of water content and hydrogels with water content reduced until 90% and 80% by means of plastic compression (nanostructuration) were generated. The biomechanical properties of the hydrogel...

The purpose of this study was to evaluate the ability of two dentin adhesive systems to induce remineralization in the bonded dentin interface after in vitro thermo-cycling. Dentin surfaces were treated with two different adhesive approaches: (1) 37% phosphoric acid (PA) plus an "etch-and-rinse" dentin adhesive (single bond, SB) (PA+SB) or (2) appl...

Objectives: The purpose of this study was to evaluate if mechanical loading promotes bioactivity at the resin interface after bonding with three different adhesive approaches. Methods: Dentin surfaces were subjected to three different treatments: demineralisation by (1) 37% phosphoric acid (PA) followed by application of an etch-and-rinse dentin...

Objectives The purpose of this study was to evaluate some physical-mechanical and morphological changes of demineralised dentine at early stages of dentine remineralisation. Methods Extracted human third molars were sectioned to obtain dentine discs. After polishing the dentine surfaces, three groups were established: 1) untreated dentine-UD), 2)...

This study evaluated the ability of different in vitro mechanical loading tests to promote new mineral formation at bonded dentin interfaces. This research demonstrated a sequential transition in the dentin remineralizing procedure through the analysis of the mineral and matrix gradients. Mechanical loading in phosphoric acid (PA)-treated samples p...

The aim of this study was to ascertain whether zinc may improve the repair ability of demineralized dentin. Dentin disks were demineralized by phosphoric acid during 15 s and immersed in artificial saliva, remineralizing solution, a zinc chloride solution and a zinc oxide solution. Dentin specimens were analyzed after 24 h and 1 month of storage. S...

Unlabelled: Design of restorative materials should be focused on promoting not only adhesion but also dentine self-repair processes. Objective: To ascertain if ZnO and ZnCl2-doped resins are materials able to induce calcium (Ca) and phosphate (P) deposition. Methods: 48 resin disks were prepared with the following materials: (1) single bond -3...

Teeth are continuously subjected to mechanical loading during mastication, swallowing and parafunctional habits. The purpose of this study was to evaluate if mechanical loading is able to promote remineralization at etched and bonded dentin interfaces. Flat mid-coronal human dentin surfaces were subjected to different treatments: (1) demineralizati...

This study reports physical and chemical changes that occur at early dentin remineralization stages. Extracted human third molars were sectioned to obtain dentin discs. After polishing the dentin surfaces, three groups were established: (1) untreated dentin (UD), (2) 37% phosphoric acid application for 15 s (partially demineralized dentin-PDD), and...

Objective: To evaluate dentin caries removal effectiveness (CRE) and minimal invasiveness potential (MIP) of carbide burs. Method: Sectioned Carious molars were photographed, before and after caries removal, using a Digital Single Lens Reflex camera. The following regions of interest were measured using visual criteria: Residual Infected Dentin (...

Objective: to determine if zinc-doped dentin adhesives may facilitate preservation of the hybrid layer (HL) overtime. Method: Human acid-etched dentin was infiltrated with Single Bond Plus (SB) -3M/ESPE-, SB doped with 10wt% ZnO nanoparticles (ZnO-SB) or SB doped with 2wt% ZnCl2 (ZnCl-SB).AFM/nanoindentation was performed to evaluate nanohardness...

Objective: To test the ability of calcium silicate-based composites containing bioactive MTA-like fillers to enhance the remineralization of apatite-depleted dentin. Method: Dentin slices (5±2 mm side, 1.0±0.1 mm thickness) obtained from caries-free human molars were demineralized in EDTA 17% (2h, 37°C). Coronal dentin surface was restored with e...

This study aimed at evaluating the therapeutic remineralising effects of innovative light-curable materials (LCMs) containing two experimental calcium silicate-based micro-fillers (TCS) modified with β-TCP only or β-TCP, zinc oxide (ZnO)/polyacrylic acid (PAA) on mineral-depleted bonded-dentine interfaces in simulated body fluids (SBFS). Three expe...

Teeth are continuously subjected to stresses during mastication, swallowing and parafunctional habits, producing a significant reduction of the bonding efficacy in adhesive restorations. The purpose of this study was to evaluate the metalloproteinases (MMPs)-mediated dentin collagen degradation of hybrid layers created by using different deminerali...

Objective: The objective of the study was to determine if zinc-doped etch-and-rinse dentin adhesive may induce therapeutic effects within the resin-dentin interface. Methods: Human acid-etched dentin was infiltrated with Adper™ Single Bond Plus (SB, 3M ESPE, St. Paul, MN, USA), SB doped with 10wt.% ZnO nanoparticles (ZnO-SB) or SB doped with 2wt...

Innovative dental restorative materials should be highly bioactive to induce therapeutic effects at the bonding interface during intimate contact with biological fluids. This study aimed at evaluating the remineralisation properties of innovative light-curable resin-based dental materials containing bioactive calcium-phosphosilicates micro-fillers....

Aim: To characterize three radiopaque Magnesium Phosphate Cements (MPCs) developed for endodontic purposes. Methodology: Three experimental MPCs containing Bi2 O3 were formulated. The experimental cements, which consisted of mixtures of magnesium oxide with different phosphate salts, were characterized for setting time, injectability, porosity,...

Purpose: To evaluate the effect of dentin pretreatment with epigallocatechin-3-gallate solution on the preservation of the resin/dentin interface with etch-and-rinse adhesives. Materials and methods: Thirty extracted human molars were prepared to expose the dentin surface and divided into 5 groups according to the pretreatment solution. Dentin s...

Objectives: Matrix metalloproteinases contribute to collagen degradation in resin-dentine interfaces. Bioactive/resin-based materials may interact with dental hard tissues and induce therapeutic remineralising effects on mineral-depleted resin-dentine interfaces. The aim aimed at determining if the inclusion of bioactive fillers within the compos...

There are some studies where particles of bioative materials were incorporated into Glass-ionomer cements and Resin-modified glass-ionomer cements (RMGICs) trying to achieve bioactivity and dental remineralization. High roughness above 200 nm of restorative materials surfaces may contribute to staining, plaque accumulation and consequent gingival i...

Objective: To assess dentine caries removal effectiveness (CRE) and minimal invasiveness potential (MIP) of carbide and polymer burs. Methods: Sectioned carious molars were photographed. Digital images were taken, before and after caries removal, using a Digital Single Lens Reflex camera. The following regions of interest were measured using vis...

Objectives: To determine effect of ageing on deciduous dentine-resin interfaces bond strength and the metalloproteinases (MMPs) activity at the hybrid layer compared to permanent dentine. Methods: Microtensile bond strength (MTBS) tests were performed in human deciduous and permanent dentine after 24h, 3 and 6 months using an etch and rinse adhe...

The objective of this article is to evaluate the resistance to degradation of resin-dentin bonds formed with three one-step adhesives. Flat, mid-coronal dentin surfaces were bonded with the self-etching adhesives [Tokuyama Bond Force (TBF), One Up Bond F Plus (OUB), and G-Bond (GB)]. The bonded teeth were subjected to fatigue loading, chemical degr...

Objectives: To determine if the addition of bioactive fillers to a dental adhesive may favour remineralisation at the resin-dentine interface. Methods: Three experimental resin adhesives were applied onto phosphoric acid etched dentine: 1) neat resin, 2) resin with 90 wt% tri-calcium silicate mixed with 10 wt% β-Tri-Calcium phosphate (βTCS), 3) res...

Objectives: Matrix metalloproteinases contribute to collagen degradation in resin-dentin interfaces. To determine if collagen degradation at the resin-dentin hybrid layer is reduced by bioactive fillers. Methods: Dentin beams were untreated, demineralized with 10% phosphoric acid for 12 h (PA) or EDTA-treated for 6 d and submitted to infiltration...

Objectives: Professional dental air-polishing is particularly indicated when pigmented conditions are encountered and in those patients who may be not able to maintain an adequate oral hygiene. This study aimed to evaluate the roughness effects induced by air-polishing treatments using bioactive glass on the surface of artificial enamel-MACOR®. M...

This study aimed to evaluate the effect of four chemomechanical surface treatments on the surface average microroughness and profile of laser-sintered and vacuum-cast dental prosthetic structures. Square-shaped blocks (10 mm × 10 mm × 1.5 mm) were prepared as follows: (1) laser-sintered CoCr (L) (ST2724G); (2) cast Co-Cr (C) (Gemium-cn); and (3) c...

Collagen dentin matrix may represent a suitable scaffold to be remineralized in the presence of bioactive materials. The purpose of this study was to determine if experimental resin cements containing bioactive fillers may modulate matrix metalloproteinase-mediated collagen degradation of etched dentin. Human dentin beams demineralized using 10% ph...

The aim of this study was to evaluate the microtensile bond strength (μTBS) and confocal micropermeability of resin bonded-dentin specimens created using two representative two-step/self-etch adhesives submitted to short-term period degradation strategies such as simulated pulpal pressure, thermo- or mechanical-cycling challenges. Clearfil SE Bond...

Objectives: To ascertain whether the addition of zinc to Single Bond adhesive affect modulus of elasticity (ME), ultimate tensile strength (UTS) and water sorption (Wsp). Methods: Six groups were prepared: 1) Single Bond (SB); 2) SB plus ZnCl2 (1 wt%); 3) SB plus ZnCl2 (2 wt%); 4) SB plus ZnO particles (5 wt%); 5) SB plus ZnO (10 wt%); 6) SB plus Z...

Objectives: To evaluate the wettability and surface roughness of enamel surfaces and microtensile bond strength (MTBS) after two different surface treatments. Methods: flat (500grit) enamel surfaces of 2mm thick from bovine incisors were divided in 2 groups: 1) application of Prompt-L-Pop (PLP) and 2) 36% Phosphoric acid etching (PAE). After grindi...

The objective of this article was to investigate the effect of carbide and polymer burs caries removal methods on the bond strength of different adhesives to dentin. Resin restorations were performed in sound and caries-affected dentin, after using polymer or carbide burs and bonding with four different adhesive (Single bond, SB; Clearfil SE bond,...

To evaluate whether fiber post surface conditioning techniques would influence the ultimate retentive strength of self-adhesive resin cements into the root canal. 50 single-rooted premolars with one root canal were endodontically treated and prepared to receive a fiber post. Five groups were formed (n=10) according to the post surface pre-treatment...

Nanofillers are added to dental adhesives to improve mechanical properties of the hybrid layer. Ethanol or water added to the demineralized dentin to improve adhesive infiltration may produce filler aggregation. To assess the effect of 5 vol% water or ethanol addition on nanoparticles distribution in dental adhesives. Six available commercial adhes...