Manuel Perez-Mateos

Manuel Perez-Mateos
Universidad de Burgos | UBU · Department of Biotechnology and Food Science

About

21
Publications
3,750
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800
Citations
Citations since 2017
1 Research Item
382 Citations
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20172018201920202021202220230204060
20172018201920202021202220230204060

Publications

Publications (21)
Article
Full-text available
In this work, barley seed coating with urease and phosphatase, free and immobilized in humic acids, was studied as a tool to enhance the use efficiency of N and P by plants. The seeds were encapsulated in gels of Ca-alginate by a method that allows gelation of the polymer, containing enzymes, from the surface of seeds outward. The coating process d...
Article
BACKGROUND Most of the N fertilizers added to the soil are not efficiently used by plants and are lost to the atmosphere or leached from the soil, causing environmental pollution and increasing cost. The barley seed encapsulation in calcium alginate gels containing free or immobilized urease was investigated to enhance plant utilization of soil N.R...
Article
A large portion of the soluble P fertilizers introduced into soil reacts with soil components forming insoluble P products, which are not efficiently taken up by plants. Barley seed (Hordeum vulgare L. 'Volley') encapsulation in Ca-alginate gels containing phosphatase free or immobilized with soil humates (humate-phosphatase complexes, HPC) was inv...
Article
Full-text available
Coating barley seeds with free and immobilized alkaline phosphatase was investigated as a potential means to enhance plant utilization of accumulated soil phosphorus (P). Two coating techniques were studied: film-coating and pelleting. The highest phosphatase activity retention in the coating layer, ranging from 0·48 to 0·67, was observed when seed...
Article
This study was designed to evaluate and compare antioxidant capacity and radical scavenging activity of naringin and its aglycone by different in vitro assays. The effects of flavanones on lipid peroxidation, glutathione (GSH) oxidation and DNA cleavage were also assessed. The results showed that naringenin exhibited higher antioxidant capacity and...
Article
An alkaline phosphatase (EC 3.1.3.1) from Escherichia coli ATCC27257 was immobilized by copolymerization with resorcinol. The phosphatase-polyresorcinol complex synthesized retained about 74% of the original enzymatic activity. The pH and temperature profile of the immobilized and free enzyme revealed a similar behavior. Kinetic parameters were det...
Article
Neutrase, a commercial preparation of Bacillus subtilis , was covalently immobilized on alginate-glutaraldehyde beads. Immobilization conditions and characterization of the immobilized enzyme were investigated. Central composite design and response surface methods were employed to evaluate the effects of immobilization parameters, such as glutarald...
Article
In this paper, a long-term investigation of the enzyme hydrolysis of pectin, using a free enzyme membrane reactor (FEMR), is presented. Production of crude enzymes (polygalacturonase and pectin lyase), in Aspergillus niger CECT 2088 cultures grown on an agricultural subproduct (apple pomace) as a low cost inducer, was also studied. Observing the re...
Article
Optimisation of pectin hydrolysis using pectolytic enzymes produced by Aspergillus niger CECT 2088 was carried out. The effect of enzyme concentration (0.018–0.072%, w/v), substrate concentration (0.004–0.896%, w/v), pH (4.49–5.91), temperature (32.2–67.8 °C) and reaction time (18.2–71.8 min) on the enzymatic process was studied. The experiments we...
Article
Capillary electrophoresis using an untreated fused-silica capillary and a low-pH buffer containing urea and hydroxypropyl methyl cellulose was used to follow the proteolytic action of neutral protease of Bacillus subtilis on isolated casein fractions and whole milk casein (CN) samples. Electrophoretic analysis showed that this protease caused inten...
Article
The effect of the ripening time on the proteolytic process in cheeses made from ewe's milk during a 139-day ripening period was monitored by the use of capillary electrophoresis of pH 4.6 insoluble fraction. Totals of 18 and 21 peaks were recognized and matched in the electropherograms obtained with a fused-silica capillary and a neutral capillary...
Article
The effect of the ripening time on the proteolytic process in cheeses manufactured from mixtures of cow's and ewe's milk during a 167-day ripening period was monitored by capillary electrophoresis of the pH 4.6-insoluble fraction. Totals of 21 and 16 peaks were recognized and matched in the electropherograms obtained with a fused-silica capillary a...
Article
Kinetic properties and thermal inactivation of pectinlyase (PL) were assayed in commercial pectinase preparations (Rapidase C80, Pectinase CCM, Pectinex 3XL and Grindamyl 3PA) by using apple pectin as substrate. The PL activity of Rapidase C80 showed substrate inhibition, while the other enzyme preparations followed typical Michaelis–Menten kinetic...
Article
Alkaline phosphatase (EC 3.1.3.1) extracted from Escherichia coli ATCC27257 was immobilised by co-flocculation with soil humates in the presence of Ca2+. The effects of time, temperature, pH and concentration of enzyme and support on immobilisation were studied. Between 58 and 92% of the added phosphatase was strongly bound to the humates, dependin...
Article
In order to optimize the enzymatic hydrolysis of three celluloses (carboxymethyl cellulose, powdered cellulose and microgranular cellulose), the effect of varying enzyme and substrate concentration, temperature and pH was studied. The best experimental conditions found to degrade the celluloses were 48 h of incubation, an enzyme concentration of fo...
Article
Three methods of wheat straw treatment (with NaOH, H2O2 and butylamine) and its subsequent saccharification by Trichoderma reesei cellulases and Aspergillus niger β-glucosidase are reported. The treatment of straw with NaOH for 1 h in the autoclave (120°C) caused a great loss of the hemicellulose content and a partial removal of lignin, provoking a...
Article
A method for determining urease activity in soil columns is described. It is based on a systematic study of factors affecting the release of ammonium when soil columns are perfused with a buffered urea solution (containing KCl to release NH4+) under steady-state conditions at a low flow velocity. Effluent emerging from the column is quickly mixed w...
Article
The soil fraction of structural microunits with diameters <50 mu m, which contain organic matter of a higher grade of humification associated with enzymes, showed the most inhibited biological activity (80.1% inhibition).-from Authors

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