Manuel Aureliano

Manuel Aureliano
Universidade do Algarve | UALG · DCBB Faculty of Sciences and Technology

PhD Biochemistry, MSc Cell Bio

About

104
Publications
11,518
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3,392
Citations
Citations since 2016
26 Research Items
2050 Citations
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
Introduction
Dr. Manuel Aureliano, Associate Professor (Aggregation) of Biochemistry, University of Algarve, Faro, Portugal. Actually, he is member of the Direction of the Portuguese Society of Biochemistry (SPB). Also belongs to the Portuguese Bioinorganic Group (BIOIN Portugal). Editor of the book “Vanadium Biochemistry”, Research SignPost, 2007, Kerala, India. Main research topics : i) The role of decavanadate in biochemistry and biology; ii) Metals oxidative stress iii) muscle contraction and stress.
Additional affiliations
January 2010 - June 2010
University of Gothenburg
Position
  • PhD co-supervision
January 1998 - December 2012
Universidade NOVA de Lisboa
January 1998 - December 2013
Universidade do Algarve

Publications

Publications (104)
Article
Decavanadate is a polyoxometalate (POMs) that has shown extensive biological activities, including antidiabetic and anticancer activity. Importantly, vanadium-based compounds as well as antidiabetic biguanide drugs, such as metformin, have shown to exert therapeutic effects in melanoma. A combination of these agents, the metformin-decavanadate comp...
Article
Full-text available
Plasma membrane calcium ATPases (PMCA) and sarco(endo) reticulum calcium ATPases (SERCA), are key proteins in the maintenance of calcium homeostasis. Herein, we compare for the first time the inhibition of SERCA and PMCA calcium pumps by several polyoxotungstates (POTs), namely by Wells-Dawson phosphotungstate anions [P2W18O62]⁶⁻ (intact, {P2W18}),...
Article
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The increase in bacterial resistance to antibiotics has led researchers to find new compounds or find combinations between different compounds with potential antibacterial action and with the ability to prevent the development of antibiotic resistance. Polyoxotungstates (POTs) are inorganic clusters that may fulfill that need, either individually o...
Article
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In this contribution, we provide an overview of gold compound applications against viruses or parasites during recent years. The special properties of gold have been the subject of intense investigation in recent years, which has led to the development of its chemistry with the synthesis of new compounds and the study of its applicability in variou...
Article
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Polyoxidovanadates (POVs, previously named polyoxovanadates) are a subgroup of polyoxidometalates (POMs, previously named polyoxometalates) with interesting pharmacological actions that have been tested as potential antidiabetic, antibacterial, antiprotozoal, antiviral, and anticancer drugs. They contain mainly vanadium and are able to interact wit...
Article
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Polyoxometalates (POMs) are clusters of units of oxoanions of transition metals, such as Mo, W, V and Nb, that can be formed upon acidification of neutral solutions. Once formed, some POMs have shown to persist in solution, even in the neutral and basic pH range. These inorganic clusters, amenable of a variety of structures, have been studied in en...
Article
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Plasma membrane calcium ATPases (PMCA) are key proteins in the maintenance of calcium (Ca2+) homeostasis. Dysregulation of PMCA function is associated with several human pathologies, including neurodegenerative diseases, and, therefore, these proteins are potential drug targets to counteract those diseases. Gold compounds, namely of Au(I), are well...
Article
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Polyoxovanadates (POVs) are a subclass of a larger family of polyanionic group V and VI metal-oxo clusters that are known as polyoxometalates (POMs). POMs have been found to have antidiabetic, antibacterial, antiprotozoal, antiviral and anticancer activities, which have sparked interest in their use as bioinorganic drugs. Among POVs, decavanadate (...
Article
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The application of metals in biological systems has been a rapidly growing branch of science. Vanadium has been investigated and reported as an anticancer agent. Melanoma is the most aggressive type of skin cancer, the incidence of which has been increasing annually worldwide. It is of paramount importance to identify novel pharmacological agents f...
Article
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The experimental data collected over the past 15 years on the interaction of decavanadate(V) (V10O286-; V10), a polyoxometalate (POM) with promising anticancer and antibacterial action, with G-actin, were rationalized by using several computational approaches (docking, density functional theory (DFT), and molecular dynamics (MD)). Moreover, a compa...
Article
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The therapeutic applications of gold are well-known for many centuries. The most used gold compounds contain Au(I). Herein, we report, for the first time, the ability of four Au(I) and Au(III) complexes, namely dichloro (2-pyridinecarboxylate) Au(III) (abbreviated as 1), chlorotrimethylphosphine Au(I) (2), 1,3-bis(2,6-diisopropylphenyl) imidazole-2...
Article
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Polyoxometalates (POMs) are of increasing interest due to their proven anticancer activities. Aquaporins (AQPs) were found to be overexpressed in tumors bringing particular attention to their inhibitors as anticancer drugs. Herein, we report for the first time the ability of polyoxotungstates (POTs), such as of Wells–Dawson P2W18, P2W12, and P2W15,...
Article
Full-text available
Polyoxometalates (POMs) are promising inorganic inhibitors for P-type ATPases. The experimental models used to study the effects of POMs on these ATPases are usually in vitro models using vesicles from several membrane sources. Very recently, some polyoxotungstates, such as the Dawson anion [P2W18O62]6−, were shown to be potent P-type ATPase inhibi...
Article
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Recently, a global analysis of the structure-activity-relationship of a series of polyoxometalates (POMs) referred that the most active POMs were ascribed to be polyoxovanadates (POVs), especially decavanadate (V10), which was very active against certain bacteria (Bijelic et al, Chem Comm, 2018). The present study explores this observation and comp...
Article
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Polyoxometalates (POMs) represent an emerging class of inorganic metal oxides, which over the last decades demonstrated promising biological activities by the virtue of their great diversity in structures and properties. They possess high potential for the inhibition of various tumor types, however, their unspecific interactions with biomolecules a...
Article
Full-text available
Polyoxometalates (POMs) represent an emerging class of inorganic metal oxides, which over the last decades demonstrated promising biological activities by the virtue of their great diversity in structures and properties. They possess high potential for the inhibition of various tumor types, however, their unspecific interactions with biomolecules a...
Article
In many diseases as well as during biological aging peroxynitrite (ONOO⁻) reacts with sarcomeric proteins, such as myosin and actin, in skeletal muscle (Steinberg, Circ. Res. 2013). We have previously demonstrated that exposure of purified myosin subfragment-1 (S1) to submicromolar fluxes of peroxynitrite produces strong inhibition of the actomyosi...
Article
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Polyoxometalates (POMs) are, mostly anionic, metal oxide compounds that span a wide range of tunable physical and chemical features rendering them very interesting for biologal purposes, an continously emerging but little explored field. Due to their biological and biochemical effects, including antitumor, -viral and –bacterial properties, POMs and...
Article
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Decavanadate−actin interactions were studied by EXAFS and XANES, allowing us to simultaneously probe, for the first time, all vanadium species in the system. V10 interacts with G-actin (at its ATP binding site), a V−SCys coordination having been verified along with oxidovanadium formation. V10’s interplay with F-actin was found to be weak and occur...
Article
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Anticancer drugs are designed to inhibit tumor cell proliferation by interacting with DNA and altering cellular growth factors. When released into the waterbodies of municipal and hospital effluents these pharmaceutical compounds may pose a risk to non-target aquatic organisms, due to their mode of action (cytotoxic, genotoxic, mutagenic and terato...
Article
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Polyoxometalates are transition metal complexes that exhibit a broad diversity of structures and properties rendering them promising for biological purposes. They are able to inhibit a series of biologically important enzymes including various phosphatases, thus affecting many biochemical processes. In the present study, we analyzed and compared th...
Article
The decaniobate ion, (Nb10=[Nb10O28]6-) being isoelectronic and isostructural with the decavanadate ion (V10=[V10O28]6-), but chemically and electrochemically more inert, has been useful in advancing the understanding of V10 toxicology and pharmacological activities. In the present study, the solution chemistry of Nb10 and V10 between pH 4 and 12 i...
Article
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This review covers recent advances in the understanding of decavanadate toxicology and pharmacological applications. Toxicological in vivo studies point out that V 10 induces several changes in several oxidative stress parameters, different from the ones observed for vanadate (V 1 ). In in vitro studies with mitochondria, a particularly potent V 10...
Chapter
Anatoxin‐a (ANTX‐a) is a toxin well known to induce neurotoxicity. There have been several synthetic pathways to obtain ANTX‐a, and these are briefly described in this chapter. It is possible to separate into categories the different total synthesis approaches to ANTX‐a, according to the methodology used: (a) Synthesis of ANTX‐a via ring expansion...
Article
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Although the number of papers about "decavanadate'' has increased in the last decade in vivo or/and cellular toxicological studies involving vanadium that takes in account the contribution of decavanadate (V-10) ion species to biological responses are scarce. These innovative in vivo studies are supported by the fact that decavanadate interact with...
Article
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Context: Immunosuppressive agents are associated with profound metabolic side effects including new-onset diabetes and dyslipidemia after organ transplantation. Objective: To investigate the effects of cyclosporine A (CsA) and tacrolimus on glucose uptake and insulin signaling in human adipocytes and their impact on the regulation of cellular tr...
Article
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Decavanadate is a polyoxometalate consisting of 10 octahedral vanadium centers, which has been found to exert biological effects and has been observed in vivo. Biological activity implies that a material is taken up into a cell or that the material interacts with membrane receptors. Because of the large size and the high molecular charge, it is non...
Article
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The putative applications of poly-, oligo- and mono-oxometalates in biochemistry, biology, pharmacology and medicine are rapidly attracting interest. In particular, these compounds may act as potent ion pump inhibitors and have the potential to play a role in the treatment of e.g. ulcers, cancer and ischemic heart disease. However, the mechanism of...
Article
Cyclosporin A (CsA), tacrolimus and rapamycin are immunosuppressive agents (IA) associated with insulin resistance and dyslipidemia, although their molecular effects on lipid metabolism in adipose tissue are unknown. We explored IAs effects on lipolysis, lipid storage and expression of genes involved on lipid metabolism in human isolated adipocytes...
Article
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Recently we demonstrated that the decavanadate (V(10)) ion is a stronger Ca(2+)-ATPase inhibitor than other oxometalates, such as the isoelectronic and isostructural decaniobate ion, and the tungstate and molybdate monomer ions, and that it binds to this protein with a 1 : 1 stoichiometry. The V(10) interaction is not affected by any of the protein...
Article
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The studies about the interaction of actin with vanadium are seldom. In the present paper the effects of vanadyl, vanadate, and decavanadate in the actin structure and function were compared. Decavanadate clearly interacts with actin, as shown by 51V-NMR spectroscopy. Decavanadate interaction with actin induces protein cysteine oxidation and vanady...
Article
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Rapamycin is an immunosuppressive agent used after organ transplantation, but its molecular effects on glucose metabolism needs further evaluation. We explored rapamycin effects on glucose uptake and insulin signalling proteins in adipocytes obtained via subcutaneous (n=62) and omental (n=10) fat biopsies in human donors. At therapeutic concentrati...
Article
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Although the number of papers about "vanadium" has doubled in the last decade, the studies about "vanadium and actin" are scarce. In the present review, the effects of vanadyl, vanadate and decavanadate on actin structure and function are compared. Decavanadate (51)V NMR signals, at -516 ppm, broadened and decreased in intensity upon actin titratio...
Article
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Recebido em 16/9/11; aceito em 2/12/11; publicado na web em 23/1/12 METALLOIDS IN ORIGIN AND EVOLUTION OF LIFE. Metalloids have characteristics between metals and non-metals which give them, in some cases, specific properties. At least two of this chemical elements, boron and silicon, are essential to a significant number of living organisms and si...
Article
Over the last few decades there has been increasing interest in oxometalate and polyoxometalate applications to medicine and pharmacology. This interest arose, at least in part, due to the properties of these classes of compounds as anti-cancer, anti-diabetic agents, and also for treatment of neurodegenerative diseases, among others. However, our u...
Article
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The number of papers about decavanadate has doubled in the past decade. In the present review, new insights into decavanadate biochemistry, cell biology, and antidiabetic and antitumor activities are described. Decameric vanadate species (V(10)) clearly differs from monomeric vanadate (V(1)), and affects differently calcium pumps, and structure and...
Article
Oxidovanadium(IV), a cationic species (VO(2+)) of vanadium(IV), binds to several proteins, including actin. Upon titration with oxidovanadium(IV), approximately 100% quenching of the intrinsic fluorescence of monomeric actin purified from rabbit skeletal muscle (G-actin) was observed, with a V(50) of 131 μM, whereas for the polymerized form of acti...
Article
A trial was undertaken to evaluate Differential Scanning Calorimetry (DSC) as a fast analytical tool to differentiate gilthead seabream subjected to variable conditions of slaughter stress and post-mortem storage. Fish were subjected to different harvesting stress conditions: profound anaesthesia (PA, low stress) and net crowding (NC, high stress)....
Article
ATP prevents G-actin cysteine oxidation and vanadyl formation specifically induced by decavanadate, suggesting that the oxometalate-protein interaction is affected by the nucleotide. The ATP exchange rate is increased by 2-fold due to the presence of decavanadate when compared with control actin (3.1×10(-3) s(-1)), and an apparent dissociation cons...
Article
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Abstract The peroxynitrite-induced functional impairment of myosin was studied in different reaction conditions, known to alter the oxidative chemistry of peroxynitrite, to better understand the molecular mechanisms of this interaction. It is shown that peroxynitrite is able to enhance the basal MgATPase activity up to 2-fold while inhibiting the a...
Article
The effects of different vanadium compounds namely pyridine-2,6-dicarboxylatedioxovanadium(V) (V5-dipic), bis(maltolato) oxovanadium(IV) (BMOV) and amavadine, and oligovanadates namely metavanadate and decavanadate were analysed on basal and insulin stimulated glucose uptake in rat adipocytes. Decavanadate (50 microM), manifest a higher increases (...
Conference Paper
Full-text available
Texture is considered to be one of the most important quality attributes in fish. Flesh texture in fish is a complex set of intrinsic traits such as the muscle chemical composition (fat content and fatty acid profile, glycogen stores) and muscle cellularity and is strongly influenced by a variety of extrinsic factors such as pre- and post-slaughter...
Article
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Currently, efforts have been directed towards using decavanadate as a tool for the understanding of several biochemical processes such as muscle contraction, calcium homeostasis, in vivo changes of oxidative stress markers, mitochondrial oxygen consumption, mitochondrial membrane depolarization, actin polymerization and glucose uptake, among others...
Article
Full-text available
Incubation of actin with decavanadate induces cysteine oxidation and oxidovanadium(IV) formation. The studies were performed combining kinetic with spectroscopic (NMR and EPR) methodologies. Although decavanadate is converted to labile oxovanadates, the rate of deoligomerization can be very slow (half-life time of 5.4 h, at 25 degrees C, with a fir...
Article
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The decameric vanadate species V(10)O(28)(6-), also referred to as decavanadate, impact proteins, lipid structures and cellular function, and show some effects in vivo on oxidative stress processes and other biological properties. The mode of action of decavanadate in many biochemical systems depends, at least in part, on the charge and size of the...
Article
The general affinity of the sarcoplasmic reticulum (SR) Ca (2+)-ATPase was examined for three different classes of vanadium coordination complexes including a vanadium(V) compound, pyridine-2,6-dicarboxylatodioxovanadium(V) (PDC-V(V)), and two vanadium(IV) compounds, bis(maltolato)oxovanadium(IV) (BMOV), and an analogue of amavadine, bis( N-hydroxy...
Article
Full-text available
Vanadium, a trace metal known to accumulate in bone and to mimic insulin, has been shown to regulate mammalian bone formation using in vitro and in vivo systems. In the present work, short- and long-term effects of metavanadate (containing monomeric, dimeric, tetrameric and pentameric vanadate species) and decavanadate (containing decameric vanadat...
Article
We recently reported proliferative and anti-mineralogenic effects of vanadate on fish chondrocytes and here we investigate the signalling pathways associated with these effects. Our data show that vanadate stimulates chondrocyte proliferation through the MAPK pathway, using signalling mechanisms similar to those used by IGF-1, while it inhibits cho...